(Circular) (Six-base) MAPSORT of: pFN21AB5805.seq Check: 9853 from: 1 to: 6118 pFN21AB5805 6118 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2954 3928 278 Size: 53 83 186 936 1418 974 2468 Fragments arranged by size: 2468 1418 974 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3461 1937 Size: 1524 4594 Acc65I G'GTAC_C Cuts at: 3439 3439 Size: 6118 AclI AA'CG_TT Cuts at: 1563 4245 4618 1563 Size: 2682 373 3063 Fragments arranged by size: 3063 2682 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4181 5565 5833 1632 Size: 2549 1384 268 1917 Fragments arranged by size: 2549 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5921 AflIII A'CryG_T Cuts at: 1203 3156 5017 5994 1203 Size: 1953 1861 977 1327 Fragments arranged by size: 1953 1861 1327 977 AgeI A'CCGG_T Cuts at: 3333 4949 3333 Size: 1616 4502 AhdI GACnn_n'nnGTC Cuts at: 4847 4847 Size: 6118 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6118 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6118 AlwNI CAG_nnn'CTG Cuts at: 3861 5433 3861 Size: 1572 4546 ApaI G_GGCC'C Cuts at: 2548 3044 2548 Size: 496 5622 ApaLI G'TGCA_C Cuts at: 4175 5331 4175 Size: 1156 4962 ApoI r'AATT_y Cuts at: 1493 3427 3653 3805 5914 1493 Size: 1934 226 152 2109 1697 Fragments arranged by size: 2109 1934 1697 226 152 AscI GG'CGCG_CC Cuts at: 2961 2961 Size: 6118 AseI AT'TA_AT Cuts at: 160 1032 4672 160 Size: 872 3640 1606 Fragments arranged by size: 3640 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 AvaI C'yCGr_G Cuts at: 1870 1943 2149 2548 2903 3043 3320 3340 3443 Size: 73 206 399 355 140 277 20 103 Cuts at: 3443 1870 Size: 4545 Fragments arranged by size: 4545 399 355 277 206 140 103 73 20 BamHI G'GATC_C Cuts at: 3448 3815 3448 Size: 367 5751 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2035 2291 2514 2836 2881 Size: 325 217 627 248 256 223 322 45 Cuts at: 2881 3097 3439 4894 618 Size: 216 342 1455 1842 Fragments arranged by size: 1842 1455 627 342 325 322 256 248 223 217 216 45 BanII G_rGCy'C Cuts at: 1391 2132 2156 2198 2548 2765 2897 3044 3360 Size: 741 24 42 350 217 132 147 316 Cuts at: 3360 3437 1391 Size: 77 4072 Fragments arranged by size: 4072 741 350 316 217 147 132 77 42 24 BbeI G_GCGC'C Cuts at: 2039 2295 2518 2840 2885 2039 Size: 256 223 322 45 5272 Fragments arranged by size: 5272 322 256 223 45 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5147 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4309 4343 1903 Size: 34 2372 34 3678 Fragments arranged by size: 3678 2372 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4010 5220 4010 Size: 1210 4908 BclI T'GATC_A Cuts at: 1548 1548 Size: 6118 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2168 3061 3083 Size: 107 122 71 631 605 496 893 22 Cuts at: 3083 4729 136 Size: 1646 1525 Fragments arranged by size: 1646 1525 893 631 605 496 122 107 71 22 BglII A'GATC_T Cuts at: 6113 6113 Size: 6118 BlpI GC'TnA_GC Cuts at: 2891 3535 2891 Size: 644 5474 Bme1580I G_kGCm'C Cuts at: 1790 2066 2548 2636 3044 3100 4179 5335 1790 Size: 276 482 88 408 56 1079 1156 2573 Fragments arranged by size: 2573 1156 1079 482 408 276 88 56 BmgBI CAC'GTC Cuts at: 1318 3015 3159 1318 Size: 1697 144 4277 Fragments arranged by size: 4277 1697 144 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3849 4807 442 Size: 416 53 83 399 24 2432 958 1753 Fragments arranged by size: 2432 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2456 1056 Size: 1400 4718 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2813 4778 1239 Size: 1574 1965 2579 Fragments arranged by size: 2579 1965 1574 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2375 2504 3008 4243 5108 5406 2375 Size: 129 504 1235 865 298 3087 Fragments arranged by size: 3087 1235 865 504 298 129 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2491 2633 3227 4781 882 Size: 617 992 142 594 1554 2219 Fragments arranged by size: 2219 1554 992 617 594 142 BsaAI yAC'GTr Cuts at: 493 1204 2674 3909 493 Size: 711 1470 1235 2702 Fragments arranged by size: 2702 1470 1235 711 BsaBI GATnn'nnATC Cuts at: 6112 6112 Size: 6118 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2036 2123 2217 2292 Size: 53 83 186 936 503 87 94 75 Cuts at: 2292 2376 2515 2837 2882 2933 2951 3402 3925 Size: 84 139 322 45 51 18 451 523 Cuts at: 3925 4307 4942 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 635 523 503 451 382 322 186 139 94 87 84 83 75 53 51 45 18 BsaWI w'CCGG_w Cuts at: 3333 4551 4949 5223 5370 5781 3333 Size: 1218 398 274 147 411 3670 Fragments arranged by size: 3670 1218 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5893 Fragments arranged by size: 5893 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2120 2831 1186 Size: 934 711 4473 Fragments arranged by size: 4473 934 711 BseYI C'CCAG_C Cuts at: 3136 5321 5817 3136 Size: 2185 496 3437 Fragments arranged by size: 3437 2185 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2654 3251 3367 1163 Size: 573 918 597 116 3914 Fragments arranged by size: 3914 918 597 573 116 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2214 2706 3075 3246 3568 4329 Size: 275 186 213 492 369 171 322 761 Cuts at: 4329 5357 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 492 369 322 275 213 186 171 BsiHKAI G_wGCw'C Cuts at: 2412 2702 4179 4264 5335 2412 Size: 290 1477 85 1071 3195 Fragments arranged by size: 3195 1477 1071 290 85 BsiWI C'GTAC_G Cuts at: 2310 2310 Size: 6118 BsmI GAATG_Cn' Cuts at: 3629 3722 3629 Size: 93 6025 BsmBI CGTCTCn'nnnn_ Cuts at: 2958 2958 Size: 6118 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2066 2132 2156 2198 2412 2548 2636 Size: 399 276 66 24 42 214 136 88 Cuts at: 2636 2702 2765 2897 3044 3100 3360 3437 4179 Size: 66 63 132 147 56 260 77 742 Cuts at: 4179 4264 5335 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 276 260 214 147 136 132 88 85 77 66 66 63 56 42 24 BspEI T'CCGG_A Cuts at: 5781 5781 Size: 6118 BspHI T'CATG_A Cuts at: 4007 5737 4007 Size: 1730 4388 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3473 844 Size: 2629 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2827 3319 4005 1394 Size: 429 1004 492 686 3507 Fragments arranged by size: 3507 1004 686 492 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4613 4787 65 Size: 1183 3365 174 1396 Fragments arranged by size: 3365 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2183 2234 2279 2288 2511 2870 3082 Size: 110 373 51 45 9 223 359 212 Cuts at: 3082 3333 4762 4949 1700 Size: 251 1429 187 2869 Fragments arranged by size: 2869 1429 373 359 251 223 212 187 110 51 45 9 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6118 BssHII G'CGCG_C Cuts at: 1925 2114 2507 2961 3309 1925 Size: 189 393 454 348 4734 Fragments arranged by size: 4734 454 393 348 189 BssSI C'ACGA_G Cuts at: 2059 2695 4178 5190 2059 Size: 636 1483 1012 2987 Fragments arranged by size: 2987 1483 1012 636 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6118 BstBI TT'CG_AA Cuts at: 4922 4922 Size: 6118 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6118 BstYI r'GATC_y Cuts at: 1972 3448 3815 4200 4217 5669 5755 5767 6113 Size: 1476 367 385 17 1452 86 12 346 Cuts at: 6113 1972 Size: 1977 Fragments arranged by size: 1977 1476 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2379 2917 3069 3246 3387 513 Size: 552 1314 538 152 177 141 3244 Fragments arranged by size: 3244 1314 552 538 177 152 141 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5379 Fragments arranged by size: 5379 683 56 BtsI GCAGTG_nn' Cuts at: 3634 4428 4448 3634 Size: 794 20 5304 Fragments arranged by size: 5304 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6118 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6083 DraI TTT'AAA Cuts at: 3422 3422 Size: 6118 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5125 809 Size: 507 3809 1802 Fragments arranged by size: 3809 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2178 2211 2382 Size: 54 1425 50 275 18 348 33 171 Cuts at: 2382 2703 2923 3000 3072 3243 3285 3565 4454 Size: 321 220 77 72 171 42 280 889 Cuts at: 4454 6083 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 348 321 280 275 220 171 171 77 72 54 50 43 42 33 18 EagI C'GGCC_G Cuts at: 1537 1812 2211 2703 3072 3243 3565 1537 Size: 275 399 492 369 171 322 4090 Fragments arranged by size: 4090 492 399 369 322 275 171 EarI CTCTTCn'nnn_ Cuts at: 1353 4048 1353 Size: 2695 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4705 5079 5225 387 Size: 1064 138 3116 374 146 1280 Fragments arranged by size: 3116 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2813 4181 4778 5565 5833 1239 Size: 393 1181 1368 597 787 268 1524 Fragments arranged by size: 1524 1368 1181 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2030 2480 2522 2030 Size: 450 42 5626 Fragments arranged by size: 5626 450 42 EcoO109I rG'GnC_Cy Cuts at: 2545 2743 2830 3262 2545 Size: 198 87 432 5401 Fragments arranged by size: 5401 432 198 87 EcoRI G'AATT_C Cuts at: 3427 3805 3427 Size: 378 5740 FspI TGC'GCA Cuts at: 4624 4624 Size: 6118 HaeII r_GCGC'y Cuts at: 1678 2039 2054 2295 2518 2840 2885 3209 5265 Size: 361 15 241 223 322 45 324 2056 Cuts at: 5265 1678 Size: 2531 Fragments arranged by size: 2531 2056 361 324 322 241 223 45 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4759 4791 4833 4865 1373 Size: 32 135 32 3187 32 42 32 2626 Fragments arranged by size: 3187 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3462 3708 669 Size: 988 281 1524 246 3079 Fragments arranged by size: 3079 1524 988 281 246 HindIII A'AGCT_T Cuts at: 748 2723 748 Size: 1975 4143 HpaI GTT'AAC Cuts at: 3708 3708 Size: 6118 KasI G'GCGC_C Cuts at: 2035 2291 2514 2836 2881 2035 Size: 256 223 322 45 5272 Fragments arranged by size: 5272 322 256 223 45 KpnI G_GTAC'C Cuts at: 3443 3443 Size: 6118 MfeI C'AATT_G Cuts at: 3717 3717 Size: 6118 MluI A'CGCG_T Cuts at: 5994 5994 Size: 6118 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2691 2997 5232 5416 1305 Size: 1386 306 2235 184 2007 Fragments arranged by size: 2235 2007 1386 306 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4296 Fragments arranged by size: 4296 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2282 2468 4076 4435 4594 518 Size: 648 1116 186 1608 359 159 2042 Fragments arranged by size: 2042 1608 1116 648 359 186 159 MspA1I CmG'CkG Cuts at: 1606 2200 2887 2919 3071 3133 3248 3534 4211 Size: 594 687 32 152 62 115 286 677 Cuts at: 4211 5359 5604 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 687 677 594 286 245 152 115 62 32 NaeI GCC'GGC Cuts at: 2236 2513 3084 2236 Size: 277 571 5270 Fragments arranged by size: 5270 571 277 NarI GG'CG_CC Cuts at: 2036 2292 2515 2837 2882 2036 Size: 256 223 322 45 5272 Fragments arranged by size: 5272 322 256 223 45 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5566 NdeI CA'TA_TG Cuts at: 387 387 Size: 6118 NgoMIV G'CCGG_C Cuts at: 2234 2511 3082 2234 Size: 277 571 5270 Fragments arranged by size: 5270 571 277 NheI G'CTAG_C Cuts at: 1052 2452 1052 Size: 1400 4718 NotI GC'GGCC_GC Cuts at: 3072 3243 3565 3072 Size: 171 322 5625 Fragments arranged by size: 5625 322 171 NspI r_CATG'y Cuts at: 1132 1229 3476 5021 1132 Size: 97 2247 1545 2229 Fragments arranged by size: 2247 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2938 1348 Size: 1590 4528 PciI A'CATG_T Cuts at: 5017 5017 Size: 6118 PflMI CCAn_nnn'nTGG Cuts at: 1108 2991 1108 Size: 1883 4235 PmeI GTTT'AAAC Cuts at: 3422 3422 Size: 6118 PmlI CAC'GTG Cuts at: 2674 2674 Size: 6118 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4187 4219 5700 5732 4187 Size: 32 1481 32 4573 Fragments arranged by size: 4573 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2743 2743 Size: 6118 PshAI GACnn'nnGTC Cuts at: 1613 2610 1613 Size: 997 5121 PsiI TTA'TAA Cuts at: 3688 3688 Size: 6118 PspOMI G'GGCC_C Cuts at: 2544 3040 2544 Size: 496 5622 PspXI vC'TCGA_Gb Cuts at: 3320 3320 Size: 6118 PstI C_TGCA'G Cuts at: 830 2205 3213 3470 830 Size: 1375 1008 257 3478 Fragments arranged by size: 3478 1375 1008 257 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6118 SacII CC_GC'GG Cuts at: 2920 3072 3249 2920 Size: 152 177 5789 Fragments arranged by size: 5789 177 152 SalI G'TCGA_C Cuts at: 1936 3460 1936 Size: 1524 4594 SanDI GG'GwC_CC Cuts at: 2743 2743 Size: 6118 SbfI CC_TGCA'GG Cuts at: 3470 3470 Size: 6118 ScaI AGT'ACT Cuts at: 2207 4366 6078 2207 Size: 2159 1712 2247 Fragments arranged by size: 2247 2159 1712 SexAI A'CCwGG_T Cuts at: 2492 2492 Size: 6118 SfcI C'TryA_G Cuts at: 826 1045 2201 3209 3466 4601 5282 5473 826 Size: 219 1156 1008 257 1135 681 191 1471 Fragments arranged by size: 1471 1156 1135 1008 681 257 219 191 SfoI GGC'GCC Cuts at: 2037 2293 2516 2838 2883 2037 Size: 256 223 322 45 5272 Fragments arranged by size: 5272 322 256 223 45 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 SgrAI Cr'CCGG_yG Cuts at: 1700 2511 1700 Size: 811 5307 SmaI CCC'GGG Cuts at: 1872 2151 3045 3342 3445 1872 Size: 279 894 297 103 4545 Fragments arranged by size: 4545 894 297 279 103 SmlI C'TyrA_G Cuts at: 820 1017 1943 2390 2519 3023 3320 4222 5123 Size: 197 926 447 129 504 297 902 901 Cuts at: 5123 5385 820 Size: 262 1553 Fragments arranged by size: 1553 926 902 901 504 447 297 262 197 129 SnaBI TAC'GTA Cuts at: 493 3909 493 Size: 3416 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6118 SphI G_CATG'C Cuts at: 1132 3476 1132 Size: 2344 3774 SrfI GCCC'GGGC Cuts at: 3045 3045 Size: 6118 SspI AAT'ATT Cuts at: 5 52 4042 5 Size: 47 3990 2081 Fragments arranged by size: 3990 2081 47 StuI AGG'CCT Cuts at: 1517 2396 1517 Size: 879 5239 StyI C'CwwG_G Cuts at: 513 1065 2433 3557 513 Size: 552 1368 1124 3074 Fragments arranged by size: 3074 1368 1124 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2495 4145 4162 4315 4500 4945 325 Size: 1255 915 1650 17 153 185 445 1498 Fragments arranged by size: 1650 1498 1255 915 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2205 4364 6076 Size: 274 80 33 51 1443 228 2159 1712 Cuts at: 6076 96 Size: 138 Fragments arranged by size: 2159 1712 1443 274 228 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2681 2713 2735 2767 3320 3352 6078 6110 2681 Size: 32 22 32 553 32 2726 32 2689 Fragments arranged by size: 2726 2689 553 32 32 32 32 22 Tth111I GACn'n_nGTC Cuts at: 1316 2857 1316 Size: 1541 4577 XbaI T'CTAG_A Cuts at: 3454 3454 Size: 6118 XhoI C'TCGA_G Cuts at: 1943 3320 1943 Size: 1377 4741 XmaI C'CCGG_G Cuts at: 1870 2149 3043 3340 3443 1870 Size: 279 894 297 103 4545 Fragments arranged by size: 4545 894 297 279 103 XmnI GAAnn'nnTTC Cuts at: 4247 4247 Size: 6118 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2952 3926 276 Size: 53 83 186 936 1418 974 2468 Fragments arranged by size: 2468 1418 974 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PsrI PvuII RsrII SacI SapI SfiI SwaI XcmI