(Linear) (Six-base) MAPSORT of: KIBB5805.seq Check: 7851 from: 1 to: 1413 KIBB5805.orf With 182 enzymes: SgfI * July 3, 2012 16:07 .. AatII G_ACGT'C Cuts at: 0 949 1413 Size: 949 464 AflIII A'CryG_T Cuts at: 0 1151 1413 Size: 1151 262 AgeI A'CCGG_T Cuts at: 0 1328 1413 Size: 1328 85 ApaI G_GGCC'C Cuts at: 0 543 1039 1413 Size: 543 496 374 Fragments arranged by size: 543 496 374 AscI GG'CGCG_CC Cuts at: 0 956 1413 Size: 956 457 AvaI C'yCGr_G Cuts at: 0 144 543 898 1038 1315 1335 1413 Size: 144 399 355 140 277 20 78 Fragments arranged by size: 399 355 277 144 140 78 20 BanI G'GyrC_C Cuts at: 0 30 286 509 831 876 1092 1413 Size: 30 256 223 322 45 216 321 Fragments arranged by size: 322 321 256 223 216 45 30 BanII G_rGCy'C Cuts at: 0 127 151 193 543 760 892 1039 1355 Size: 127 24 42 350 217 132 147 316 Cuts at: 1355 1413 Size: 58 Fragments arranged by size: 350 316 217 147 132 127 58 42 24 BbeI G_GCGC'C Cuts at: 0 34 290 513 835 880 1413 Size: 34 256 223 322 45 533 Fragments arranged by size: 533 322 256 223 45 34 BglI GCCn_nnn'nGGC Cuts at: 0 163 1056 1078 1413 Size: 163 893 22 335 Fragments arranged by size: 893 335 163 22 BlpI GC'TnA_GC Cuts at: 0 886 1413 Size: 886 527 Bme1580I G_kGCm'C Cuts at: 0 61 543 631 1039 1095 1413 Size: 61 482 88 408 56 318 Fragments arranged by size: 482 408 318 88 61 56 BmgBI CAC'GTC Cuts at: 0 1010 1154 1413 Size: 1010 144 259 Fragments arranged by size: 1010 259 144 BmtI G_CTAG'C Cuts at: 0 451 1413 Size: 451 962 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 808 1413 Size: 808 605 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 370 499 1003 1413 Size: 370 129 504 410 Fragments arranged by size: 504 410 370 129 BsaI GGTCTCn'nnnn_ Cuts at: 0 486 628 1222 1413 Size: 486 142 594 191 Fragments arranged by size: 594 486 191 142 BsaAI yAC'GTr Cuts at: 0 669 1413 Size: 669 744 BsaHI Gr'CG_yC Cuts at: 0 31 118 212 287 371 510 832 877 Size: 31 87 94 75 84 139 322 45 Cuts at: 877 928 946 1397 1413 Size: 51 18 451 16 Fragments arranged by size: 451 322 139 94 87 84 75 51 45 31 18 16 BsaWI w'CCGG_w Cuts at: 0 1328 1413 Size: 1328 85 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 115 826 1413 Size: 115 711 587 Fragments arranged by size: 711 587 115 BseYI C'CCAG_C Cuts at: 0 1131 1413 Size: 1131 282 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 649 1246 1362 1413 Size: 649 597 116 51 Fragments arranged by size: 649 597 116 51 BsiEI CG_ry'CG Cuts at: 0 209 701 1070 1241 1413 Size: 209 492 369 171 172 Fragments arranged by size: 492 369 209 172 171 BsiHKAI G_wGCw'C Cuts at: 0 407 697 1413 Size: 407 290 716 Fragments arranged by size: 716 407 290 BsiWI C'GTAC_G Cuts at: 0 305 1413 Size: 305 1108 BsmBI CGTCTCn'nnnn_ Cuts at: 0 953 1413 Size: 953 460 Bsp1286I G_dGCh'C Cuts at: 0 61 127 151 193 407 543 631 697 Size: 61 66 24 42 214 136 88 66 Cuts at: 697 760 892 1039 1095 1355 1413 Size: 63 132 147 56 260 58 Fragments arranged by size: 260 214 147 136 132 88 66 66 63 61 58 56 42 24 BsrBI CCG'CTC Cuts at: 0 822 1314 1413 Size: 822 492 99 Fragments arranged by size: 822 492 99 BsrFI r'CCGG_y Cuts at: 0 178 229 274 283 506 865 1077 1328 Size: 178 51 45 9 223 359 212 251 Cuts at: 1328 1413 Size: 85 Fragments arranged by size: 359 251 223 212 178 85 51 45 9 BssHII G'CGCG_C Cuts at: 0 109 502 956 1304 1413 Size: 109 393 454 348 109 Fragments arranged by size: 454 393 348 109 109 BssSI C'ACGA_G Cuts at: 0 54 690 1413 Size: 54 636 723 Fragments arranged by size: 723 636 54 BtgI C'CryG_G Cuts at: 0 374 912 1064 1241 1382 1413 Size: 374 538 152 177 141 31 Fragments arranged by size: 538 374 177 152 141 31 EaeI y'GGCC_r Cuts at: 0 173 206 377 698 918 995 1067 1238 Size: 173 33 171 321 220 77 72 171 Cuts at: 1238 1280 1413 Size: 42 133 Fragments arranged by size: 321 220 173 171 171 133 77 72 42 33 EagI C'GGCC_G Cuts at: 0 206 698 1067 1238 1413 Size: 206 492 369 171 175 Fragments arranged by size: 492 369 206 175 171 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 808 1413 Size: 808 605 EcoNI CCTnn'n_nnAGG Cuts at: 0 25 475 517 1413 Size: 25 450 42 896 Fragments arranged by size: 896 450 42 25 EcoO109I rG'GnC_Cy Cuts at: 0 540 738 825 1257 1413 Size: 540 198 87 432 156 Fragments arranged by size: 540 432 198 156 87 HaeII r_GCGC'y Cuts at: 0 34 49 290 513 835 880 1204 1413 Size: 34 15 241 223 322 45 324 209 Fragments arranged by size: 324 322 241 223 209 45 34 15 HindIII A'AGCT_T Cuts at: 0 718 1413 Size: 718 695 KasI G'GCGC_C Cuts at: 0 30 286 509 831 876 1413 Size: 30 256 223 322 45 537 Fragments arranged by size: 537 322 256 223 45 30 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 686 992 1413 Size: 686 306 421 Fragments arranged by size: 686 421 306 MslI CAynn'nnrTG Cuts at: 0 277 463 1413 Size: 277 186 950 Fragments arranged by size: 950 277 186 MspA1I CmG'CkG Cuts at: 0 195 882 914 1066 1128 1243 1413 Size: 195 687 32 152 62 115 170 Fragments arranged by size: 687 195 170 152 115 62 32 NaeI GCC'GGC Cuts at: 0 231 508 1079 1413 Size: 231 277 571 334 Fragments arranged by size: 571 334 277 231 NarI GG'CG_CC Cuts at: 0 31 287 510 832 877 1413 Size: 31 256 223 322 45 536 Fragments arranged by size: 536 322 256 223 45 31 NgoMIV G'CCGG_C Cuts at: 0 229 506 1077 1413 Size: 229 277 571 336 Fragments arranged by size: 571 336 277 229 NheI G'CTAG_C Cuts at: 0 447 1413 Size: 447 966 NotI GC'GGCC_GC Cuts at: 0 1067 1238 1413 Size: 1067 171 175 Fragments arranged by size: 1067 175 171 PasI CC'CwG_GG Cuts at: 0 933 1413 Size: 933 480 PflMI CCAn_nnn'nTGG Cuts at: 0 986 1413 Size: 986 427 PmlI CAC'GTG Cuts at: 0 669 1413 Size: 669 744 PpuMI rG'GwC_Cy Cuts at: 0 738 1413 Size: 738 675 PshAI GACnn'nnGTC Cuts at: 0 605 1413 Size: 605 808 PspOMI G'GGCC_C Cuts at: 0 539 1035 1413 Size: 539 496 378 Fragments arranged by size: 539 496 378 PspXI vC'TCGA_Gb Cuts at: 0 1315 1413 Size: 1315 98 PstI C_TGCA'G Cuts at: 0 200 1208 1413 Size: 200 1008 205 Fragments arranged by size: 1008 205 200 SacII CC_GC'GG Cuts at: 0 915 1067 1244 1413 Size: 915 152 177 169 Fragments arranged by size: 915 177 169 152 SanDI GG'GwC_CC Cuts at: 0 738 1413 Size: 738 675 ScaI AGT'ACT Cuts at: 0 202 1413 Size: 202 1211 SexAI A'CCwGG_T Cuts at: 0 487 1413 Size: 487 926 SfcI C'TryA_G Cuts at: 0 196 1204 1413 Size: 196 1008 209 Fragments arranged by size: 1008 209 196 SfoI GGC'GCC Cuts at: 0 32 288 511 833 878 1413 Size: 32 256 223 322 45 535 Fragments arranged by size: 535 322 256 223 45 32 SgrAI Cr'CCGG_yG Cuts at: 0 506 1413 Size: 506 907 SmaI CCC'GGG Cuts at: 0 146 1040 1337 1413 Size: 146 894 297 76 Fragments arranged by size: 894 297 146 76 SmlI C'TyrA_G Cuts at: 0 385 514 1018 1315 1413 Size: 385 129 504 297 98 Fragments arranged by size: 504 385 297 129 98 SrfI GCCC'GGGC Cuts at: 0 1040 1413 Size: 1040 373 StuI AGG'CCT Cuts at: 0 391 1413 Size: 391 1022 StyI C'CwwG_G Cuts at: 0 428 1413 Size: 428 985 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 490 1413 Size: 490 923 TatI w'GTAC_w Cuts at: 0 200 1413 Size: 200 1213 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 676 708 730 762 1315 1347 1413 Size: 676 32 22 32 553 32 66 Fragments arranged by size: 676 553 66 32 32 32 22 Tth111I GACn'n_nGTC Cuts at: 0 852 1413 Size: 852 561 XhoI C'TCGA_G Cuts at: 0 1315 1413 Size: 1315 98 XmaI C'CCGG_G Cuts at: 0 144 1038 1335 1413 Size: 144 894 297 78 Fragments arranged by size: 894 297 144 78 ZraI GAC'GTC Cuts at: 0 947 1413 Size: 947 466 Enzymes that do cut: AatII AflIII AgeI ApaI AscI AvaI BanI BanII BbeI BglI BlpI Bme1580I BmgBI BmtI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BsrBI BsrFI BssHII BssSI BtgI EaeI EagI Eco57MI EcoNI EcoO109I HaeII HindIII KasI MmeI MslI MspA1I NaeI NarI NgoMIV NheI NotI PasI PflMI PmlI PpuMI PshAI PspOMI PspXI PstI SacII SanDI ScaI SexAI SfcI SfoI SgrAI SmaI SmlI SrfI StuI StyI TaqII TatI TstI Tth111I XhoI XmaI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AcuI AfeI AflII AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BmrI BplI Bpu10I BsaBI BsaXI BsmI BspEI BspHI BspMI BsrDI BsrGI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HpaI KpnI MfeI MluI MscI NcoI NdeI NruI NsiI NspI PacI PciI PfoI PmeI PpiI PsiI PsrI PvuI PvuII RsrII SacI SalI SapI SbfI SfiI SgfI SnaBI SpeI SphI SspI SwaI XbaI XcmI XmnI