################################## # NO CUT ENZYMES ( 88 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI Alw44I ApaLI AscI Asp718I AspI AviII AvrII BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BpuAI BsaAI BsaBI BseAI BsiWI BsmBI BsmI Bsp1407I BspEI BspLU11I BsrDI BsrGI BssSI BstAPI BstXI BstYI CpoI DraIII DrdI EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HpaI KpnI MamI MfeI MflI MluI MroI MunI NcoI NheI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacI SalI SapI SbfI SexAI SnaBI SpeI SphI Sse8387I SspBI StyI Tth111I XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AhdI 1900 GACGGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 2131 GGATC GGATC(4/5) AocI 835 CCTGAGG CC^T[ACGT]AGG Aor51HI 137 AGCGCT AGC^GCT AspEI 1900 GACGGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1166 GTGCTC G[AT]GC[AT]^C BalI 414 TGGCCA TGG^CCA BlpI 914 GCTTAGC GC^T[ACGT]AGC Bpu1102I 914 GCTTAGC GC^T[ACGT]AGC BsaWI 1896 ACCGGA [AT]^CCGG[AT] BseRI 358 CTCCTC GAGGAG(10/8) BsgI 664 CTGCAC GTGCAG(16/14) BsiHKAI 1166 GTGCTC G[AT]GC[AT]^C BspDI 1414 ATCGAT AT^CGAT BspHI 2049 TCATGA T^CATGA Bst1107I 1793 GTATAC GTA^TAC BstEII 399 GGTGACC G^GT[ACGT]ACC BstF5I 1305 GGATG GGATG(2/0) BstPI 399 GGTGACC G^GT[ACGT]ACC BstZ17I 1793 GTATAC GTA^TAC Bsu36I 835 CCTGAGG CC^T[ACGT]AGG CelII 914 GCTTAGC GC^T[ACGT]AGC ClaI 1414 ATCGAT AT^CGAT Eam1105I 1900 GACGGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1075 GAAGAG CTCTTC(1/4) Eco47III 137 AGCGCT AGC^GCT Eco81I 835 CCTGAGG CC^T[ACGT]AGG EcoNI 1144 CCTGTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 399 GGTGACC G^GT[ACGT]ACC FokI 1305 GGATG GGATG(9/13) HgaI 581 GCGTC GACGC(5/10) HincII 2268 GTTGAC GT[CT]^[GA]AC HindII 2268 GTTGAC GT[CT]^[GA]AC Ksp632I 1075 GAAGAG CTCTTC(1/4) MaeII 1883 ACGT A^CGT MluNI 414 TGGCCA TGG^CCA MscI 414 TGGCCA TGG^CCA MslI 1028 CATTACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeI 761 CATATG CA^TATG NspI 1820 GCATGT [GA]CATG^[CT] PpuMI 22 AGGACCT [GA]G^G[AT]CC[CT] PstI 2073 CTGCAG CTGCA^G RcaI 2049 TCATGA T^CATGA ScaI 861 AGTACT AGT^ACT SfiI 633 GGCCCGGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 348 CGCCGGCG C[GA]^CCGG[CT]G SmaI 635 CCCGGG CCC^GGG SspI 1707 AATATT AAT^ATT StuI 1002 AGGCCT AGG^CCT SwaI 800 ATTTAAAT ATTT^AAAT XbaI 2302 TCTAGA T^CTAGA XmaCI 635 CCCGGG C^CCGGG XmaI 635 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AcyI 259 GGCGCC G[GA]^CG[CT]C 346 GGCGCC ApaI 233 GGGCCC GGGCC^C 632 GGGCCC AseI 1410 ATTAAT AT^TAAT 1611 ATTAAT AsnI 1410 ATTAAT AT^TAAT 1611 ATTAAT Asp700I 1240 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2152 GAAAACCTTC BanI 259 GGCGCC G^G[CT][GA]CC 346 GGCGCC BbeI 259 GGCGCC GGCGC^C 346 GGCGCC BglI 634 GCCCGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 700 GCCTGCGAGGC BsaHI 259 GGCGCC G[GA]^CG[CT]C 346 GGCGCC BsaI 2306 GAGACC GGTCTC(1/5) 769 GGTCTC BsmFI 476 GGGAC GGGAC(10/14) 520 GGGAC BspMI 939 ACCTGC ACCTGC(4/8) 2178 ACCTGC BsrBI 628 GAGCGG CCGCTC(-3/-3) 752 GAGCGG BssHII 117 GCGCGC G^CGCGC 197 GCGCGC BstBI 1784 TTCGAA TT^CGAA 2105 TTCGAA DpnII 1522 GATC ^GATC 2132 GATC Hin1I 259 GGCGCC G[GA]^CG[CT]C 346 GGCGCC HindIII 1155 AAGCTT A^AGCTT 1554 AAGCTT KasI 259 GGCGCC G^GCGCC 346 GGCGCC MboI 1522 GATC ^GATC 2132 GATC NaeI 349 GCCGGC GCC^GGC 643 GCCGGC NarI 259 GGCGCC GG^CGCC 346 GGCGCC NdeII 1522 GATC ^GATC 2132 GATC NgoMIV 349 GCCGGC G^CCGGC 643 GCCGGC NotI 121 GCGGCCGC GC^GGCCGC 531 GCGGCCGC NspV 1784 TTCGAA TT^CGAA 2105 TTCGAA PflMI 105 CCATCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 300 CCACGGGCTGG PshBI 1410 ATTAAT AT^TAAT 1611 ATTAAT Sau3AI 1522 GATC ^GATC 2132 GATC SfcI 2073 CTGCAG C^T[GA][CT]AG 2213 CTGTAG SfoI 259 GGCGCC GGC^GCC 346 GGCGCC SfuI 1784 TTCGAA TT^CGAA 2105 TTCGAA SmlI 1507 CTTGAG C^T[CT][GA]AG 2066 CTCAAG Van91I 105 CCATCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 300 CCACGGGCTGG XmnI 1240 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2152 GAAAACCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwNI ApoI AvaI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaJI BsiEI BsiYI BslI BsmAI BsoBI Bsp1286I BsrFI BsrI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DraI DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI KspI MaeI MaeIII MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PleI PvuII RsaI SacII Sau96I ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I