(Circular) (Six-base) MAPSORT of: pFN21AE4222.seq Check: 5877 from: 1 to: 7024 pFN21AE4222 7024 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2953 2953 Size: 7024 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4834 278 Size: 53 83 186 936 3298 2468 Fragments arranged by size: 3298 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2847 3799 4221 4367 1937 Size: 910 952 422 146 4594 Fragments arranged by size: 4594 952 910 422 146 Acc65I G'GTAC_C Cuts at: 4345 4345 Size: 7024 AclI AA'CG_TT Cuts at: 1563 5151 5524 1563 Size: 3588 373 3063 Fragments arranged by size: 3588 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2216 2541 2553 2560 2836 2897 3032 3126 Size: 584 325 12 7 276 61 135 94 Cuts at: 3126 4109 4148 5087 6471 6739 1632 Size: 983 39 939 1384 268 1917 Fragments arranged by size: 1917 1384 983 939 584 325 276 268 135 94 61 39 12 7 AfeI AGC'GCT Cuts at: 2144 2144 Size: 7024 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6827 AflIII A'CryG_T Cuts at: 1203 5923 6900 1203 Size: 4720 977 1327 Fragments arranged by size: 4720 1327 977 AgeI A'CCGG_T Cuts at: 5855 5855 Size: 7024 AhdI GACnn_n'nnGTC Cuts at: 3910 5753 3910 Size: 1843 5181 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7024 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7024 AlwNI CAG_nnn'CTG Cuts at: 2342 2430 3149 3248 4767 6339 2342 Size: 88 719 99 1519 1572 3027 Fragments arranged by size: 3027 1572 1519 719 99 88 ApaI G_GGCC'C Cuts at: 2242 2641 2242 Size: 399 6625 ApaLI G'TGCA_C Cuts at: 5081 6237 5081 Size: 1156 5868 ApoI r'AATT_y Cuts at: 1493 2748 3193 4093 4119 4333 4559 4711 6820 Size: 1255 445 900 26 214 226 152 2109 Cuts at: 6820 1493 Size: 1697 Fragments arranged by size: 2109 1697 1255 900 445 226 214 152 26 AseI AT'TA_AT Cuts at: 160 1032 3416 3617 5578 160 Size: 872 2384 201 1961 1606 Fragments arranged by size: 2384 1961 1606 872 201 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7024 AvaI C'yCGr_G Cuts at: 1870 1943 2252 2640 2657 4145 4349 1870 Size: 73 309 388 17 1488 204 4545 Fragments arranged by size: 4545 1488 388 309 204 73 17 BamHI G'GATC_C Cuts at: 4354 4721 4354 Size: 367 6657 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2264 2351 4345 5800 618 Size: 325 217 627 477 87 1994 1455 1842 Fragments arranged by size: 1994 1842 1455 627 477 325 217 87 BanII G_rGCy'C Cuts at: 1391 2242 2364 2641 4343 1391 Size: 851 122 277 1702 4072 Fragments arranged by size: 4072 1702 851 277 122 BbeI G_GCGC'C Cuts at: 2268 2355 2268 Size: 87 6937 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6053 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5215 5249 1903 Size: 34 3278 34 3678 Fragments arranged by size: 3678 3278 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4916 6126 4916 Size: 1210 5814 BclI T'GATC_A Cuts at: 1548 1548 Size: 7024 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2645 2711 5635 Size: 107 122 71 631 605 973 66 2924 Cuts at: 5635 136 Size: 1525 Fragments arranged by size: 2924 1525 973 631 605 122 107 71 66 BglII A'GATC_T Cuts at: 7019 7019 Size: 7024 BlpI GC'TnA_GC Cuts at: 2920 4441 2920 Size: 1521 5503 Bme1580I G_kGCm'C Cuts at: 1790 2242 2415 2641 5085 6241 1790 Size: 452 173 226 2444 1156 2573 Fragments arranged by size: 2573 2444 1156 452 226 173 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7024 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2724 4755 5713 Size: 416 53 83 399 24 1307 2031 958 Cuts at: 5713 442 Size: 1753 Fragments arranged by size: 2031 1753 1307 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7024 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2138 2228 2338 3046 5684 1239 Size: 899 90 110 708 2638 2579 Fragments arranged by size: 2638 2579 899 708 110 90 Bpu10I CC'TnA_GC Cuts at: 2393 3388 2393 Size: 995 6029 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3533 4056 5149 6014 6312 3533 Size: 523 1093 865 298 4245 Fragments arranged by size: 4245 1093 865 523 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2780 4305 5687 882 Size: 617 1281 1525 1382 2219 Fragments arranged by size: 2219 1525 1382 1281 617 BsaAI yAC'GTr Cuts at: 493 1204 4815 493 Size: 711 3611 2702 Fragments arranged by size: 3611 2702 711 BsaBI GATnn'nnATC Cuts at: 7018 7018 Size: 7024 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2265 2352 4831 5213 Size: 53 83 186 936 732 87 2479 382 Cuts at: 5213 5848 275 Size: 635 1451 Fragments arranged by size: 2479 1451 936 732 635 382 186 87 83 53 BsaWI w'CCGG_w Cuts at: 3901 5457 5855 6129 6276 6687 3901 Size: 1556 398 274 147 411 4238 Fragments arranged by size: 4238 1556 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3577 3607 1178 Size: 30 165 30 2174 30 4595 Fragments arranged by size: 4595 2174 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2354 1186 Size: 1168 5856 BseYI C'CCAG_C Cuts at: 2059 2145 2182 2311 6227 6723 2059 Size: 86 37 129 3916 496 2360 Fragments arranged by size: 3916 2360 496 129 86 37 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2654 1163 Size: 573 918 5533 Fragments arranged by size: 5533 918 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2130 2540 2649 4474 5235 6263 Size: 275 186 129 410 109 1825 761 1028 Cuts at: 6263 1540 Size: 2301 Fragments arranged by size: 2301 1825 1028 761 410 275 186 129 109 BsiHKAI G_wGCw'C Cuts at: 3175 5085 5170 6241 3175 Size: 1910 85 1071 3958 Fragments arranged by size: 3958 1910 1071 85 BsmI GAATG_Cn' Cuts at: 4535 4628 4535 Size: 93 6931 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2242 2364 2415 2641 3175 4343 5085 Size: 399 452 122 51 226 534 1168 742 Cuts at: 5085 5170 6241 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1168 1071 742 534 452 399 226 122 85 51 BspEI T'CCGG_A Cuts at: 6687 6687 Size: 7024 BspHI T'CATG_A Cuts at: 4054 4913 6643 4054 Size: 859 1730 4435 Fragments arranged by size: 4435 1730 859 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2953 4192 4379 844 Size: 2109 1239 187 3489 Fragments arranged by size: 3489 2109 1239 187 BsrBI CCG'CTC Cuts at: 1394 1823 2635 2759 4911 1394 Size: 429 812 124 2152 3507 Fragments arranged by size: 3507 2152 812 429 124 BsrDI GCAATG_nn' Cuts at: 65 1248 5519 5693 65 Size: 1183 4271 174 1396 Fragments arranged by size: 4271 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2079 2295 2354 2401 2484 2648 5668 Size: 110 269 216 59 47 83 164 3020 Cuts at: 5668 5855 1700 Size: 187 2869 Fragments arranged by size: 3020 2869 269 216 187 164 110 83 59 47 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7024 BssHII G'CGCG_C Cuts at: 1925 2122 2202 1925 Size: 197 80 6747 Fragments arranged by size: 6747 197 80 BssSI C'ACGA_G Cuts at: 5084 6096 5084 Size: 1012 6012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7024 BstBI TT'CG_AA Cuts at: 3790 4111 5828 3790 Size: 321 1717 4986 Fragments arranged by size: 4986 1717 321 BstEII G'GTnAC_C Cuts at: 1184 2404 1184 Size: 1220 5804 BstYI r'GATC_y Cuts at: 1972 4354 4721 5106 5123 6575 6661 6673 7019 Size: 2382 367 385 17 1452 86 12 346 Cuts at: 7019 1972 Size: 1977 Fragments arranged by size: 2382 1977 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3800 3800 Size: 7024 Bsu36I CC'TnA_GG Cuts at: 2841 2841 Size: 7024 BtgI C'CryG_G Cuts at: 513 1065 2130 2164 2233 2305 2534 513 Size: 552 1065 34 69 72 229 5003 Fragments arranged by size: 5003 1065 552 229 72 69 34 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2545 2889 487 Size: 683 56 1319 344 4622 Fragments arranged by size: 4622 1319 683 344 56 BtsI GCAGTG_nn' Cuts at: 3981 4540 5334 5354 3981 Size: 559 794 20 5651 Fragments arranged by size: 5651 794 559 20 ClaI AT'CG_AT Cuts at: 1554 3420 1554 Size: 1866 5158 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6989 DraI TTT'AAA Cuts at: 2788 2808 3228 3954 4328 2788 Size: 20 420 726 374 5484 Fragments arranged by size: 5484 726 420 374 20 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6031 809 Size: 507 4715 1802 Fragments arranged by size: 4715 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2107 2127 2188 Size: 54 1425 50 275 18 277 20 61 Cuts at: 2188 2244 2419 2537 2550 2604 2646 2697 4471 Size: 56 175 118 13 54 42 51 1774 Cuts at: 4471 5360 6989 8 Size: 889 1629 43 Fragments arranged by size: 1774 1629 1425 889 277 275 175 118 61 56 54 54 51 50 43 42 20 18 13 EagI C'GGCC_G Cuts at: 1537 1812 2127 2537 2646 4471 1537 Size: 275 315 410 109 1825 4090 Fragments arranged by size: 4090 1825 410 315 275 109 EarI CTCTTCn'nnn_ Cuts at: 1353 3075 4954 1353 Size: 1722 1879 3423 Fragments arranged by size: 3423 1879 1722 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2150 2291 5611 5985 6131 387 Size: 1064 138 561 141 3320 374 146 1280 Fragments arranged by size: 3320 1280 1064 561 374 146 141 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2138 2216 2228 2338 2541 2553 2560 Size: 393 506 78 12 110 203 12 7 Cuts at: 2560 2836 2897 3032 3046 3126 4109 4148 5087 Size: 276 61 135 14 80 983 39 939 Cuts at: 5087 5684 6471 6739 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 983 939 787 597 506 393 276 268 203 135 110 80 78 61 39 14 12 12 7 EcoNI CCTnn'n_nnAGG Cuts at: 3153 3153 Size: 7024 EcoO109I rG'GnC_Cy Cuts at: 2028 2238 2239 2692 2028 Size: 210 1 453 6360 Fragments arranged by size: 6360 453 210 1 EcoRI G'AATT_C Cuts at: 4333 4711 4333 Size: 378 6646 FspI TGC'GCA Cuts at: 5530 5530 Size: 7024 HaeII r_GCGC'y Cuts at: 1678 2066 2146 2183 2228 2268 2343 2355 2683 Size: 388 80 37 45 40 75 12 328 Cuts at: 2683 2717 6171 1678 Size: 34 3454 2531 Fragments arranged by size: 3454 2531 388 328 80 75 45 40 37 34 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3074 3106 3629 3661 3896 Size: 32 135 32 1502 32 523 32 235 Cuts at: 3896 3928 4024 4056 5665 5697 5739 5771 1373 Size: 32 96 32 1609 32 42 32 2626 Fragments arranged by size: 2626 1609 1502 523 235 135 96 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4275 4368 4614 669 Size: 988 281 2337 93 246 3079 Fragments arranged by size: 3079 2337 988 281 246 93 HindIII A'AGCT_T Cuts at: 748 3160 3559 748 Size: 2412 399 4213 Fragments arranged by size: 4213 2412 399 HpaI GTT'AAC Cuts at: 4614 4614 Size: 7024 KasI G'GCGC_C Cuts at: 2264 2351 2264 Size: 87 6937 KpnI G_GTAC'C Cuts at: 4349 4349 Size: 7024 MfeI C'AATT_G Cuts at: 4623 4623 Size: 7024 MluI A'CGCG_T Cuts at: 6900 6900 Size: 7024 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2118 3588 3910 6138 6322 1305 Size: 813 1470 322 2228 184 2007 Fragments arranged by size: 2228 2007 1470 813 322 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2421 10 Size: 54 1425 343 589 4613 Fragments arranged by size: 4613 1425 589 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3037 4982 5341 5500 518 Size: 648 1871 1945 359 159 2042 Fragments arranged by size: 2042 1945 1871 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2106 2132 2166 2235 2439 2466 2536 3604 Size: 500 26 34 69 204 27 70 1068 Cuts at: 3604 4083 4237 4440 5117 6265 6510 1606 Size: 479 154 203 677 1148 245 2120 Fragments arranged by size: 2120 1148 1068 677 500 479 245 204 203 154 70 69 34 27 26 NaeI GCC'GGC Cuts at: 2356 2650 2356 Size: 294 6730 NarI GG'CG_CC Cuts at: 2265 2352 2265 Size: 87 6937 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6472 NdeI CA'TA_TG Cuts at: 387 2767 387 Size: 2380 4644 NgoMIV G'CCGG_C Cuts at: 2354 2648 2354 Size: 294 6730 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7024 NotI GC'GGCC_GC Cuts at: 2127 2537 4471 2127 Size: 410 1934 4680 Fragments arranged by size: 4680 1934 410 NspI r_CATG'y Cuts at: 1132 1229 3829 4382 5927 1132 Size: 97 2600 553 1545 2229 Fragments arranged by size: 2600 2229 1545 553 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7024 PciI A'CATG_T Cuts at: 5923 5923 Size: 7024 PflMI CCAn_nnn'nTGG Cuts at: 1108 2116 2311 1108 Size: 1008 195 5821 Fragments arranged by size: 5821 1008 195 PfoI T'CCnGG_A Cuts at: 3023 3023 Size: 7024 PmeI GTTT'AAAC Cuts at: 4328 4328 Size: 7024 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5093 5125 6606 6638 5093 Size: 32 1481 32 5479 Fragments arranged by size: 5479 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2028 2028 Size: 7024 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7024 PsiI TTA'TAA Cuts at: 4594 4594 Size: 7024 PspOMI G'GGCC_C Cuts at: 2238 2637 2238 Size: 399 6625 PstI C_TGCA'G Cuts at: 830 4082 4376 830 Size: 3252 294 3478 Fragments arranged by size: 3478 3252 294 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7024 PvuII CAG'CTG Cuts at: 3604 4083 4237 3604 Size: 479 154 6391 Fragments arranged by size: 6391 479 154 SacII CC_GC'GG Cuts at: 2133 2167 2236 2537 2133 Size: 34 69 301 6620 Fragments arranged by size: 6620 301 69 34 SalI G'TCGA_C Cuts at: 1936 4366 1936 Size: 2430 4594 SbfI CC_TGCA'GG Cuts at: 4376 4376 Size: 7024 ScaI AGT'ACT Cuts at: 2868 5272 6984 2868 Size: 2404 1712 2908 Fragments arranged by size: 2908 2404 1712 SfcI C'TryA_G Cuts at: 826 1045 4078 4218 4372 5507 6188 6379 826 Size: 219 3033 140 154 1135 681 191 1471 Fragments arranged by size: 3033 1471 1135 681 219 191 154 140 SfiI GGCCn_nnn'nGGCC Cuts at: 2645 2645 Size: 7024 SfoI GGC'GCC Cuts at: 2266 2353 2266 Size: 87 6937 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7024 SgrAI Cr'CCGG_yG Cuts at: 1700 2354 1700 Size: 654 6370 SmaI CCC'GGG Cuts at: 1872 2642 4351 1872 Size: 770 1709 4545 Fragments arranged by size: 4545 1709 770 SmlI C'TyrA_G Cuts at: 820 1017 1943 3512 4071 5128 6029 6291 820 Size: 197 926 1569 559 1057 901 262 1553 Fragments arranged by size: 1569 1553 1057 926 901 559 262 197 SnaBI TAC'GTA Cuts at: 493 4815 493 Size: 4322 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7024 SphI G_CATG'C Cuts at: 1132 4382 1132 Size: 3250 3774 SrfI GCCC'GGGC Cuts at: 2642 2642 Size: 7024 SspI AAT'ATT Cuts at: 5 52 3714 4948 5 Size: 47 3662 1234 2081 Fragments arranged by size: 3662 2081 1234 47 StuI AGG'CCT Cuts at: 1517 3009 1517 Size: 1492 5532 StyI C'CwwG_G Cuts at: 513 1065 4463 513 Size: 552 3398 3074 Fragments arranged by size: 3398 3074 552 SwaI ATTT'AAAT Cuts at: 2808 2808 Size: 7024 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5051 5068 5221 5406 5851 325 Size: 1255 3471 17 153 185 445 1498 Fragments arranged by size: 3471 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2866 2959 3041 Size: 274 80 33 51 1443 889 93 82 Cuts at: 3041 5270 6982 96 Size: 2229 1712 138 Fragments arranged by size: 2229 1712 1443 889 274 138 93 82 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3577 3609 3900 3932 6984 7016 3577 Size: 32 291 32 3052 32 3585 Fragments arranged by size: 3585 3052 291 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7024 XbaI T'CTAG_A Cuts at: 4307 4360 4307 Size: 53 6971 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7024 XmaI C'CCGG_G Cuts at: 1870 2640 4349 1870 Size: 770 1709 4545 Fragments arranged by size: 4545 1709 770 XmnI GAAnn'nnTTC Cuts at: 3249 4161 5153 3249 Size: 912 992 5120 Fragments arranged by size: 5120 992 912 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4832 276 Size: 53 83 186 936 3298 2468 Fragments arranged by size: 3298 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI XcmI