(Linear) (Six-base) MAPSORT of: KIEE4222.seq Check: 6743 from: 1 to: 2319 KIEE4222.orf With 182 enzymes: SgfI * July 3, 2012 18:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 948 2319 Size: 948 1371 AccI GT'mk_AC Cuts at: 0 842 1794 2216 2319 Size: 842 952 422 103 Fragments arranged by size: 952 842 422 103 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 211 536 548 555 831 892 1027 1121 Size: 211 325 12 7 276 61 135 94 Cuts at: 1121 2104 2143 2319 Size: 983 39 176 Fragments arranged by size: 983 325 276 211 176 135 94 61 39 12 7 AfeI AGC'GCT Cuts at: 0 139 2319 Size: 139 2180 AhdI GACnn_n'nnGTC Cuts at: 0 1905 2319 Size: 1905 414 AlwNI CAG_nnn'CTG Cuts at: 0 337 425 1144 1243 2319 Size: 337 88 719 99 1076 Fragments arranged by size: 1076 719 337 99 88 ApaI G_GGCC'C Cuts at: 0 237 636 2319 Size: 237 399 1683 Fragments arranged by size: 1683 399 237 ApoI r'AATT_y Cuts at: 0 743 1188 2088 2114 2319 Size: 743 445 900 26 205 Fragments arranged by size: 900 743 445 205 26 AseI AT'TA_AT Cuts at: 0 1411 1612 2319 Size: 1411 201 707 Fragments arranged by size: 1411 707 201 AvaI C'yCGr_G Cuts at: 0 247 635 652 2140 2319 Size: 247 388 17 1488 179 Fragments arranged by size: 1488 388 247 179 17 BanI G'GyrC_C Cuts at: 0 259 346 2319 Size: 259 87 1973 Fragments arranged by size: 1973 259 87 BanII G_rGCy'C Cuts at: 0 237 359 636 2319 Size: 237 122 277 1683 Fragments arranged by size: 1683 277 237 122 BbeI G_GCGC'C Cuts at: 0 263 350 2319 Size: 263 87 1969 Fragments arranged by size: 1969 263 87 BglI GCCn_nnn'nGGC Cuts at: 0 640 706 2319 Size: 640 66 1613 Fragments arranged by size: 1613 640 66 BlpI GC'TnA_GC Cuts at: 0 915 2319 Size: 915 1404 Bme1580I G_kGCm'C Cuts at: 0 237 410 636 2319 Size: 237 173 226 1683 Fragments arranged by size: 1683 237 226 173 BmrI ACTGGGnnnn_n' Cuts at: 0 719 2319 Size: 719 1600 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 133 223 333 1041 2319 Size: 133 90 110 708 1278 Fragments arranged by size: 1278 708 133 110 90 Bpu10I CC'TnA_GC Cuts at: 0 388 1383 2319 Size: 388 995 936 Fragments arranged by size: 995 936 388 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1528 2051 2319 Size: 1528 523 268 Fragments arranged by size: 1528 523 268 BsaI GGTCTCn'nnnn_ Cuts at: 0 775 2300 2319 Size: 775 1525 19 Fragments arranged by size: 1525 775 19 BsaHI Gr'CG_yC Cuts at: 0 260 347 2319 Size: 260 87 1972 Fragments arranged by size: 1972 260 87 BsaWI w'CCGG_w Cuts at: 0 1896 2319 Size: 1896 423 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1572 1602 2319 Size: 1572 30 717 Fragments arranged by size: 1572 717 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 349 2319 Size: 349 1970 BseYI C'CCAG_C Cuts at: 0 54 140 177 306 2319 Size: 54 86 37 129 2013 Fragments arranged by size: 2013 129 86 54 37 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 649 2319 Size: 649 1670 BsiEI CG_ry'CG Cuts at: 0 125 535 644 2319 Size: 125 410 109 1675 Fragments arranged by size: 1675 410 125 109 BsiHKAI G_wGCw'C Cuts at: 0 1170 2319 Size: 1170 1149 Bsp1286I G_dGCh'C Cuts at: 0 237 359 410 636 1170 2319 Size: 237 122 51 226 534 1149 Fragments arranged by size: 1149 534 237 226 122 51 BspHI T'CATG_A Cuts at: 0 2049 2319 Size: 2049 270 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 948 2187 2319 Size: 948 1239 132 Fragments arranged by size: 1239 948 132 BsrBI CCG'CTC Cuts at: 0 630 754 2319 Size: 630 124 1565 Fragments arranged by size: 1565 630 124 BsrFI r'CCGG_y Cuts at: 0 74 290 349 396 479 643 2319 Size: 74 216 59 47 83 164 1676 Fragments arranged by size: 1676 216 164 83 74 59 47 BssHII G'CGCG_C Cuts at: 0 117 197 2319 Size: 117 80 2122 Fragments arranged by size: 2122 117 80 BstBI TT'CG_AA Cuts at: 0 1785 2106 2319 Size: 1785 321 213 Fragments arranged by size: 1785 321 213 BstEII G'GTnAC_C Cuts at: 0 399 2319 Size: 399 1920 BstZ17I GTA'TAC Cuts at: 0 1795 2319 Size: 1795 524 Bsu36I CC'TnA_GG Cuts at: 0 836 2319 Size: 836 1483 BtgI C'CryG_G Cuts at: 0 125 159 228 300 529 2319 Size: 125 34 69 72 229 1790 Fragments arranged by size: 1790 229 125 72 69 34 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 540 884 2319 Size: 540 344 1435 Fragments arranged by size: 1435 540 344 BtsI GCAGTG_nn' Cuts at: 0 1976 2319 Size: 1976 343 ClaI AT'CG_AT Cuts at: 0 1415 2319 Size: 1415 904 DraI TTT'AAA Cuts at: 0 783 803 1223 1949 2319 Size: 783 20 420 726 370 Fragments arranged by size: 783 726 420 370 20 EaeI y'GGCC_r Cuts at: 0 102 122 183 239 414 532 545 599 Size: 102 20 61 56 175 118 13 54 Cuts at: 599 641 692 2319 Size: 42 51 1627 Fragments arranged by size: 1627 175 118 102 61 56 54 51 42 20 13 EagI C'GGCC_G Cuts at: 0 122 532 641 2319 Size: 122 410 109 1678 Fragments arranged by size: 1678 410 122 109 EarI CTCTTCn'nnn_ Cuts at: 0 1070 2319 Size: 1070 1249 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 145 286 2319 Size: 145 141 2033 Fragments arranged by size: 2033 145 141 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 133 211 223 333 536 548 555 831 Size: 133 78 12 110 203 12 7 276 Cuts at: 831 892 1027 1041 1121 2104 2143 2319 Size: 61 135 14 80 983 39 176 Fragments arranged by size: 983 276 203 176 135 133 110 80 78 61 39 14 12 12 7 EcoNI CCTnn'n_nnAGG Cuts at: 0 1148 2319 Size: 1148 1171 EcoO109I rG'GnC_Cy Cuts at: 0 23 233 234 687 2319 Size: 23 210 1 453 1632 Fragments arranged by size: 1632 453 210 23 1 HaeII r_GCGC'y Cuts at: 0 61 141 178 223 263 338 350 678 Size: 61 80 37 45 40 75 12 328 Cuts at: 678 712 2319 Size: 34 1607 Fragments arranged by size: 1607 328 80 75 61 45 40 37 34 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1069 1101 1624 1656 1891 1923 2019 2051 Size: 1069 32 523 32 235 32 96 32 Cuts at: 2051 2319 Size: 268 Fragments arranged by size: 1069 523 268 235 96 32 32 32 32 HincII GTy'rAC Cuts at: 0 2270 2319 Size: 2270 49 HindIII A'AGCT_T Cuts at: 0 1155 1554 2319 Size: 1155 399 765 Fragments arranged by size: 1155 765 399 KasI G'GCGC_C Cuts at: 0 259 346 2319 Size: 259 87 1973 Fragments arranged by size: 1973 259 87 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 113 1583 1905 2319 Size: 113 1470 322 414 Fragments arranged by size: 1470 414 322 113 MscI TGG'CCA Cuts at: 0 416 2319 Size: 416 1903 MslI CAynn'nnrTG Cuts at: 0 1032 2319 Size: 1032 1287 MspA1I CmG'CkG Cuts at: 0 101 127 161 230 434 461 531 1599 Size: 101 26 34 69 204 27 70 1068 Cuts at: 1599 2078 2232 2319 Size: 479 154 87 Fragments arranged by size: 1068 479 204 154 101 87 70 69 34 27 26 NaeI GCC'GGC Cuts at: 0 351 645 2319 Size: 351 294 1674 Fragments arranged by size: 1674 351 294 NarI GG'CG_CC Cuts at: 0 260 347 2319 Size: 260 87 1972 Fragments arranged by size: 1972 260 87 NdeI CA'TA_TG Cuts at: 0 762 2319 Size: 762 1557 NgoMIV G'CCGG_C Cuts at: 0 349 643 2319 Size: 349 294 1676 Fragments arranged by size: 1676 349 294 NotI GC'GGCC_GC Cuts at: 0 122 532 2319 Size: 122 410 1787 Fragments arranged by size: 1787 410 122 NspI r_CATG'y Cuts at: 0 1824 2319 Size: 1824 495 PflMI CCAn_nnn'nTGG Cuts at: 0 111 306 2319 Size: 111 195 2013 Fragments arranged by size: 2013 195 111 PfoI T'CCnGG_A Cuts at: 0 1018 2319 Size: 1018 1301 PpuMI rG'GwC_Cy Cuts at: 0 23 2319 Size: 23 2296 PspOMI G'GGCC_C Cuts at: 0 233 632 2319 Size: 233 399 1687 Fragments arranged by size: 1687 399 233 PstI C_TGCA'G Cuts at: 0 2077 2319 Size: 2077 242 PvuII CAG'CTG Cuts at: 0 1599 2078 2232 2319 Size: 1599 479 154 87 Fragments arranged by size: 1599 479 154 87 SacII CC_GC'GG Cuts at: 0 128 162 231 532 2319 Size: 128 34 69 301 1787 Fragments arranged by size: 1787 301 128 69 34 ScaI AGT'ACT Cuts at: 0 863 2319 Size: 863 1456 SfcI C'TryA_G Cuts at: 0 2073 2213 2319 Size: 2073 140 106 Fragments arranged by size: 2073 140 106 SfiI GGCCn_nnn'nGGCC Cuts at: 0 640 2319 Size: 640 1679 SfoI GGC'GCC Cuts at: 0 261 348 2319 Size: 261 87 1971 Fragments arranged by size: 1971 261 87 SgrAI Cr'CCGG_yG Cuts at: 0 349 2319 Size: 349 1970 SmaI CCC'GGG Cuts at: 0 637 2319 Size: 637 1682 SmlI C'TyrA_G Cuts at: 0 1507 2066 2319 Size: 1507 559 253 Fragments arranged by size: 1507 559 253 SrfI GCCC'GGGC Cuts at: 0 637 2319 Size: 637 1682 SspI AAT'ATT Cuts at: 0 1709 2319 Size: 1709 610 StuI AGG'CCT Cuts at: 0 1004 2319 Size: 1004 1315 SwaI ATTT'AAAT Cuts at: 0 803 2319 Size: 803 1516 TatI w'GTAC_w Cuts at: 0 861 954 1036 2319 Size: 861 93 82 1283 Fragments arranged by size: 1283 861 93 82 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1572 1604 1895 1927 2319 Size: 1572 32 291 32 392 Fragments arranged by size: 1572 392 291 32 32 XbaI T'CTAG_A Cuts at: 0 2302 2319 Size: 2302 17 XmaI C'CCGG_G Cuts at: 0 635 2319 Size: 635 1684 XmnI GAAnn'nnTTC Cuts at: 0 1244 2156 2319 Size: 1244 912 163 Fragments arranged by size: 1244 912 163 Enzymes that do cut: AarI AccI AcuI AfeI AhdI AlwNI ApaI ApoI AseI AvaI BanI BanII BbeI BglI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BssHII BstBI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NotI NspI PflMI PfoI PpuMI PspOMI PstI PvuII SacII ScaI SfcI SfiI SfoI SgrAI SmaI SmlI SrfI SspI StuI SwaI TatI TstI XbaI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AflII AflIII AgeI AleI AlfI AloI ApaLI AscI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmtI BplI BsaAI BsaBI BsiWI BsmI BsmBI BspEI BsrDI BsrGI BssSI BstAPI BstXI BstYI CspCI DraIII DrdI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HpaI KpnI MfeI MluI NcoI NheI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI SbfI SexAI SgfI SnaBI SpeI SphI StyI TaqII Tth111I XcmI XhoI ZraI