(Circular) (Six-base) MAPSORT of: pFN21AE4167.seq Check: 4403 from: 1 to: 6562 pFN21AE4167 6562 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3399 3399 Size: 6562 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4372 278 Size: 53 83 186 936 2836 2468 Fragments arranged by size: 2836 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3905 1937 Size: 1968 4594 Acc65I G'GTAC_C Cuts at: 3883 3883 Size: 6562 AclI AA'CG_TT Cuts at: 1563 3861 4689 5062 1563 Size: 2298 828 373 3063 Fragments arranged by size: 3063 2298 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3098 4625 6009 6277 1632 Size: 1466 1527 1384 268 1917 Fragments arranged by size: 1917 1527 1466 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6365 AflIII A'CryG_T Cuts at: 1203 2367 2940 3180 5461 6438 1203 Size: 1164 573 240 2281 977 1327 Fragments arranged by size: 2281 1327 1164 977 573 240 AgeI A'CCGG_T Cuts at: 5393 5393 Size: 6562 AhdI GACnn_n'nnGTC Cuts at: 5291 5291 Size: 6562 AleI CACnn'nnGTG Cuts at: 1166 2055 3335 1166 Size: 889 1280 4393 Fragments arranged by size: 4393 1280 889 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3589 1184 Size: 2405 4157 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3638 3670 3638 Size: 32 6530 AlwNI CAG_nnn'CTG Cuts at: 4305 5877 4305 Size: 1572 4990 ApaI G_GGCC'C Cuts at: 3489 3587 3489 Size: 98 6464 ApaLI G'TGCA_C Cuts at: 3184 3293 4619 5775 3184 Size: 109 1326 1156 3971 Fragments arranged by size: 3971 1326 1156 109 ApoI r'AATT_y Cuts at: 1493 3871 4097 4249 6358 1493 Size: 2378 226 152 2109 1697 Fragments arranged by size: 2378 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5116 160 Size: 872 4084 1606 Fragments arranged by size: 4084 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6562 AvaI C'yCGr_G Cuts at: 1870 1943 3580 3887 1870 Size: 73 1637 307 4545 Fragments arranged by size: 4545 1637 307 73 BamHI G'GATC_C Cuts at: 3892 4259 3892 Size: 367 6195 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2201 2798 2999 3623 3883 Size: 325 217 627 414 597 201 624 260 Cuts at: 3883 5338 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 624 597 414 325 260 217 201 BanII G_rGCy'C Cuts at: 1391 2028 3489 3587 3819 3881 1391 Size: 637 1461 98 232 62 4072 Fragments arranged by size: 4072 1461 637 232 98 62 BbeI G_GCGC'C Cuts at: 3627 3627 Size: 6562 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5591 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2120 2154 2981 3015 4753 4787 1903 Size: 34 183 34 827 34 1738 34 3678 Fragments arranged by size: 3678 1738 827 183 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4454 5664 4454 Size: 1210 5352 BclI T'GATC_A Cuts at: 1548 1548 Size: 6562 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2962 3558 3582 Size: 107 122 71 631 605 1290 596 24 Cuts at: 3582 3800 3804 5173 136 Size: 218 4 1369 1525 Fragments arranged by size: 1525 1369 1290 631 605 596 218 122 107 71 24 4 BglII A'GATC_T Cuts at: 6557 6557 Size: 6562 BlpI GC'TnA_GC Cuts at: 3979 3979 Size: 6562 Bme1580I G_kGCm'C Cuts at: 1790 3104 3188 3297 3489 3587 4623 5779 1790 Size: 1314 84 109 192 98 1036 1156 2573 Fragments arranged by size: 2573 1314 1156 1036 192 109 98 84 BmgBI CAC'GTC Cuts at: 1318 2110 1318 Size: 792 5770 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4293 5251 442 Size: 416 53 83 399 24 2876 958 1753 Fragments arranged by size: 2876 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6562 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2291 2366 2396 3665 3716 5222 1239 Size: 1052 75 30 1269 51 1506 2579 Fragments arranged by size: 2579 1506 1269 1052 75 51 30 Bpu10I CC'TnA_GC Cuts at: 2696 2817 3629 2696 Size: 121 812 5629 Fragments arranged by size: 5629 812 121 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3293 4687 5552 5850 3293 Size: 1394 865 298 4005 Fragments arranged by size: 4005 1394 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2731 5225 882 Size: 617 1232 2494 2219 Fragments arranged by size: 2494 2219 1232 617 BsaAI yAC'GTr Cuts at: 493 1204 3835 4353 493 Size: 711 2631 518 2702 Fragments arranged by size: 2702 2631 711 518 BsaBI GATnn'nnATC Cuts at: 6556 6556 Size: 6562 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3624 4369 4751 5386 Size: 53 83 186 936 2091 745 382 635 Cuts at: 5386 275 Size: 1451 Fragments arranged by size: 2091 1451 936 745 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4995 5393 5667 5814 6225 4995 Size: 398 274 147 411 5332 Fragments arranged by size: 5332 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2120 2150 2339 2369 2693 Size: 30 165 30 717 30 189 30 324 Cuts at: 2693 2723 3638 3668 3824 3854 1178 Size: 30 915 30 156 30 3886 Fragments arranged by size: 3886 915 717 324 189 165 156 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2423 2471 2483 2486 2489 2498 2501 2891 Size: 1237 48 12 3 3 9 3 390 Cuts at: 2891 2894 2903 3674 1186 Size: 3 9 771 4074 Fragments arranged by size: 4074 1237 771 390 48 12 9 9 3 3 3 3 BseYI C'CCAG_C Cuts at: 2808 3535 3587 5765 6261 2808 Size: 727 52 2178 496 3109 Fragments arranged by size: 3109 2178 727 496 52 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2089 2144 3377 1163 Size: 573 353 55 1233 4348 Fragments arranged by size: 4348 1233 573 353 55 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3445 4012 4773 5801 1540 Size: 275 186 1444 567 761 1028 2301 Fragments arranged by size: 2301 1444 1028 761 567 275 186 BsiHKAI G_wGCw'C Cuts at: 2028 2115 3188 3297 4623 4708 5779 2028 Size: 87 1073 109 1326 85 1071 2811 Fragments arranged by size: 2811 1326 1073 1071 109 87 85 BsmI GAATG_Cn' Cuts at: 2034 4073 4166 2034 Size: 2039 93 4430 Fragments arranged by size: 4430 2039 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2115 3104 3188 3297 3489 3587 Size: 399 238 87 989 84 109 192 98 Cuts at: 3587 3819 3881 4623 4708 5779 1391 Size: 232 62 742 85 1071 2174 Fragments arranged by size: 2174 1071 989 742 399 238 232 192 109 98 87 85 84 62 BspEI T'CCGG_A Cuts at: 6225 6225 Size: 6562 BspHI T'CATG_A Cuts at: 4451 6181 4451 Size: 1730 4832 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2961 3399 3917 844 Size: 2117 438 518 3489 Fragments arranged by size: 3489 2117 518 438 BsrBI CCG'CTC Cuts at: 1394 1823 2151 3256 3649 4449 1394 Size: 429 328 1105 393 800 3507 Fragments arranged by size: 3507 1105 800 429 393 328 BsrDI GCAATG_nn' Cuts at: 65 1248 2317 5057 5231 65 Size: 1183 1069 2740 174 1396 Fragments arranged by size: 2740 1396 1183 1069 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2198 3794 3803 3821 5206 5393 1700 Size: 110 388 1596 9 18 1385 187 2869 Fragments arranged by size: 2869 1596 1385 388 187 110 18 9 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6562 BssHII G'CGCG_C Cuts at: 1925 3685 3687 1925 Size: 1760 2 4800 Fragments arranged by size: 4800 1760 2 BssSI C'ACGA_G Cuts at: 4622 5634 4622 Size: 1012 5550 BstAPI GCAn_nnn'nTGC Cuts at: 794 3192 3276 794 Size: 2398 84 4080 Fragments arranged by size: 4080 2398 84 BstBI TT'CG_AA Cuts at: 5366 5366 Size: 6562 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6562 BstXI CCAn_nnnn'nTGG Cuts at: 2344 2781 3031 2344 Size: 437 250 5875 Fragments arranged by size: 5875 437 250 BstYI r'GATC_y Cuts at: 1972 2349 2845 3892 4259 4644 4661 6113 6199 Size: 377 496 1047 367 385 17 1452 86 Cuts at: 6199 6211 6557 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1047 496 385 377 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2250 2250 Size: 6562 BtgI C'CryG_G Cuts at: 513 1065 2004 2826 3143 513 Size: 552 939 822 317 3932 Fragments arranged by size: 3932 939 822 552 317 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5823 Fragments arranged by size: 5823 683 56 BtsI GCAGTG_nn' Cuts at: 2062 3104 3274 4078 4872 4892 2062 Size: 1042 170 804 794 20 3732 Fragments arranged by size: 3732 1042 804 794 170 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6562 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6527 DraI TTT'AAA Cuts at: 3866 3866 Size: 6562 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5569 809 Size: 507 4253 1802 Fragments arranged by size: 4253 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2653 2783 Size: 54 1425 50 275 18 177 646 130 Cuts at: 2783 3442 3550 3738 3801 4009 4898 6527 8 Size: 659 108 188 63 208 889 1629 43 Fragments arranged by size: 1629 1425 889 659 646 275 208 188 177 130 108 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3442 4009 1537 Size: 275 1630 567 4090 Fragments arranged by size: 4090 1630 567 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2460 2475 3047 3060 3485 4492 1353 Size: 1107 15 572 13 425 1007 3423 Fragments arranged by size: 3423 1107 1007 572 425 15 13 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2999 5149 5523 5669 387 Size: 1064 138 1410 2150 374 146 1280 Fragments arranged by size: 2150 1410 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2026 2026 Size: 6562 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2291 2366 2396 3098 3665 3716 4625 Size: 393 659 75 30 702 567 51 909 Cuts at: 4625 5222 6009 6277 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 909 787 702 659 597 567 393 268 75 51 30 EcoO109I rG'GnC_Cy Cuts at: 2062 2963 2972 3156 3584 3718 2062 Size: 901 9 184 428 134 4906 Fragments arranged by size: 4906 901 428 184 134 9 EcoRI G'AATT_C Cuts at: 3871 4249 3871 Size: 378 6184 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3532 3532 Size: 6562 FseI GG_CCGG'CC Cuts at: 3807 3807 Size: 6562 FspI TGC'GCA Cuts at: 3323 5068 3323 Size: 1745 4817 FspAI rTGC'GCAy Cuts at: 3323 3323 Size: 6562 HaeII r_GCGC'y Cuts at: 1678 3412 3617 3627 5709 1678 Size: 1734 205 10 2082 2531 Fragments arranged by size: 2531 2082 1734 205 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2569 2601 5203 5235 5277 Size: 32 135 32 997 32 2602 32 42 Cuts at: 5277 5309 1373 Size: 32 2626 Fragments arranged by size: 2626 2602 997 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3906 4152 669 Size: 988 281 1968 246 3079 Fragments arranged by size: 3079 1968 988 281 246 HindIII A'AGCT_T Cuts at: 748 2186 748 Size: 1438 5124 HpaI GTT'AAC Cuts at: 4152 4152 Size: 6562 KasI G'GCGC_C Cuts at: 3623 3623 Size: 6562 KpnI G_GTAC'C Cuts at: 3887 3887 Size: 6562 MfeI C'AATT_G Cuts at: 4161 4161 Size: 6562 MluI A'CGCG_T Cuts at: 6438 6438 Size: 6562 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5676 5860 1305 Size: 4371 184 2007 Fragments arranged by size: 4371 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2655 2785 10 Size: 54 1425 343 177 646 130 3787 Fragments arranged by size: 3787 1425 646 343 177 130 54 MslI CAynn'nnrTG Cuts at: 518 1166 2055 3181 3335 4520 4879 5038 518 Size: 648 889 1126 154 1185 359 159 2042 Fragments arranged by size: 2042 1185 1126 889 648 359 159 154 MspA1I CmG'CkG Cuts at: 1606 2306 2556 2812 3145 3569 3694 3775 3978 Size: 700 250 256 333 424 125 81 203 Cuts at: 3978 4655 5803 6048 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 700 677 424 333 256 250 245 203 125 81 NaeI GCC'GGC Cuts at: 2200 3796 3805 3823 2200 Size: 1596 9 18 4939 Fragments arranged by size: 4939 1596 18 9 NarI GG'CG_CC Cuts at: 3624 3624 Size: 6562 NcoI C'CATG_G Cuts at: 513 1065 2004 2826 513 Size: 552 939 822 4249 Fragments arranged by size: 4249 939 822 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6562 NgoMIV G'CCGG_C Cuts at: 2198 3794 3803 3821 2198 Size: 1596 9 18 4939 Fragments arranged by size: 4939 1596 18 9 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6562 NotI GC'GGCC_GC Cuts at: 3442 4009 3442 Size: 567 5995 NspI r_CATG'y Cuts at: 1132 1229 2944 3184 3238 3322 3920 5465 1132 Size: 97 1715 240 54 84 598 1545 2229 Fragments arranged by size: 2229 1715 1545 598 240 97 84 54 PasI CC'CwG_GG Cuts at: 1348 2616 3812 1348 Size: 1268 1196 4098 Fragments arranged by size: 4098 1268 1196 PciI A'CATG_T Cuts at: 2940 3180 5461 2940 Size: 240 2281 4041 Fragments arranged by size: 4041 2281 240 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6562 PfoI T'CCnGG_A Cuts at: 2268 2670 2268 Size: 402 6160 PmeI GTTT'AAAC Cuts at: 3866 3866 Size: 6562 PmlI CAC'GTG Cuts at: 3835 3835 Size: 6562 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3638 3670 4631 4663 6144 6176 3638 Size: 32 961 32 1481 32 4024 Fragments arranged by size: 4024 1481 961 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2062 2972 3156 2062 Size: 910 184 5468 Fragments arranged by size: 5468 910 184 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6562 PsiI TTA'TAA Cuts at: 4132 4132 Size: 6562 PspOMI G'GGCC_C Cuts at: 3485 3583 3485 Size: 98 6464 PstI C_TGCA'G Cuts at: 830 2171 3914 830 Size: 1341 1743 3478 Fragments arranged by size: 3478 1743 1341 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6562 PvuII CAG'CTG Cuts at: 2556 2812 3569 3694 2556 Size: 256 757 125 5424 Fragments arranged by size: 5424 757 256 125 SacI G_AGCT'C Cuts at: 2028 2028 Size: 6562 SacII CC_GC'GG Cuts at: 3146 3146 Size: 6562 SalI G'TCGA_C Cuts at: 1936 3904 1936 Size: 1968 4594 SanDI GG'GwC_CC Cuts at: 2972 2972 Size: 6562 SapI GCTCTTCn'nnn_ Cuts at: 3060 3485 3060 Size: 425 6137 SbfI CC_TGCA'GG Cuts at: 3914 3914 Size: 6562 ScaI AGT'ACT Cuts at: 2177 4810 6522 2177 Size: 2633 1712 2217 Fragments arranged by size: 2633 2217 1712 SfcI C'TryA_G Cuts at: 826 1045 2167 3910 5045 5726 5917 826 Size: 219 1122 1743 1135 681 191 1471 Fragments arranged by size: 1743 1471 1135 1122 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3804 3804 Size: 6562 SfoI GGC'GCC Cuts at: 3625 3625 Size: 6562 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6562 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6562 SmaI CCC'GGG Cuts at: 1872 3582 3889 1872 Size: 1710 307 4545 Fragments arranged by size: 4545 1710 307 SmlI C'TyrA_G Cuts at: 820 1017 1943 3308 4666 5567 5829 820 Size: 197 926 1365 1358 901 262 1553 Fragments arranged by size: 1553 1365 1358 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4353 493 Size: 3860 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6562 SphI G_CATG'C Cuts at: 1132 3920 1132 Size: 2788 3774 SspI AAT'ATT Cuts at: 5 52 4486 5 Size: 47 4434 2081 Fragments arranged by size: 4434 2081 47 StuI AGG'CCT Cuts at: 1517 2600 3039 3089 1517 Size: 1083 439 50 4990 Fragments arranged by size: 4990 1083 439 50 StyI C'CwwG_G Cuts at: 513 1065 2004 2715 2826 3084 3618 3785 4001 Size: 552 939 711 111 258 534 167 216 Cuts at: 4001 513 Size: 3074 Fragments arranged by size: 3074 939 711 552 534 258 216 167 111 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3041 4589 4606 4759 4944 5389 325 Size: 1255 1461 1548 17 153 185 445 1498 Fragments arranged by size: 1548 1498 1461 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2175 4808 6520 Size: 274 80 33 51 1443 198 2633 1712 Cuts at: 6520 96 Size: 138 Fragments arranged by size: 2633 1712 1443 274 198 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2339 2371 3824 3856 6522 6554 2339 Size: 32 1453 32 2666 32 2347 Fragments arranged by size: 2666 2347 1453 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2240 1316 Size: 924 5638 XbaI T'CTAG_A Cuts at: 3898 3898 Size: 6562 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3604 3604 Size: 6562 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6562 XmaI C'CCGG_G Cuts at: 1870 3580 3887 1870 Size: 1710 307 4545 Fragments arranged by size: 4545 1710 307 XmnI GAAnn'nnTTC Cuts at: 2189 3677 4691 2189 Size: 1488 1014 4060 Fragments arranged by size: 4060 1488 1014 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4370 276 Size: 53 83 186 936 2836 2468 Fragments arranged by size: 2836 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV NruI NsiI PacI PspXI PsrI RsrII SexAI SrfI SwaI