(Linear) (Six-base) MAPSORT of: KIEE4167.seq Check: 5444 from: 1 to: 1857 KIEE4167.orf With 182 enzymes: SgfI * July 3, 2012 18:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1394 1857 Size: 1394 463 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1093 1857 Size: 1093 764 AflIII A'CryG_T Cuts at: 0 362 935 1175 1857 Size: 362 573 240 682 Fragments arranged by size: 682 573 362 240 AleI CACnn'nnGTG Cuts at: 0 50 1330 1857 Size: 50 1280 527 Fragments arranged by size: 1280 527 50 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1584 1857 Size: 1584 273 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1633 1665 1857 Size: 1633 32 192 Fragments arranged by size: 1633 192 32 ApaI G_GGCC'C Cuts at: 0 1484 1582 1857 Size: 1484 98 275 Fragments arranged by size: 1484 275 98 ApaLI G'TGCA_C Cuts at: 0 1179 1288 1857 Size: 1179 109 569 Fragments arranged by size: 1179 569 109 AvaI C'yCGr_G Cuts at: 0 1575 1857 Size: 1575 282 BanI G'GyrC_C Cuts at: 0 196 793 994 1618 1857 Size: 196 597 201 624 239 Fragments arranged by size: 624 597 239 201 196 BanII G_rGCy'C Cuts at: 0 23 1484 1582 1814 1857 Size: 23 1461 98 232 43 Fragments arranged by size: 1461 232 98 43 23 BbeI G_GCGC'C Cuts at: 0 1622 1857 Size: 1622 235 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 115 149 976 1010 1857 Size: 115 34 827 34 847 Fragments arranged by size: 847 827 115 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 957 1553 1577 1795 1799 1857 Size: 957 596 24 218 4 58 Fragments arranged by size: 957 596 218 58 24 4 Bme1580I G_kGCm'C Cuts at: 0 1099 1183 1292 1484 1582 1857 Size: 1099 84 109 192 98 275 Fragments arranged by size: 1099 275 192 109 98 84 BmgBI CAC'GTC Cuts at: 0 105 1857 Size: 105 1752 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 286 361 391 1660 1711 1857 Size: 286 75 30 1269 51 146 Fragments arranged by size: 1269 286 146 75 51 30 Bpu10I CC'TnA_GC Cuts at: 0 691 812 1624 1857 Size: 691 121 812 233 Fragments arranged by size: 812 691 233 121 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1288 1857 Size: 1288 569 BsaI GGTCTCn'nnnn_ Cuts at: 0 726 1857 Size: 726 1131 BsaAI yAC'GTr Cuts at: 0 1830 1857 Size: 1830 27 BsaHI Gr'CG_yC Cuts at: 0 1619 1857 Size: 1619 238 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 115 145 334 364 688 718 1633 1663 Size: 115 30 189 30 324 30 915 30 Cuts at: 1663 1819 1849 1857 Size: 156 30 8 Fragments arranged by size: 915 324 189 156 115 30 30 30 30 30 8 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 418 466 478 481 484 493 496 886 Size: 418 48 12 3 3 9 3 390 Cuts at: 886 889 898 1669 1857 Size: 3 9 771 188 Fragments arranged by size: 771 418 390 188 48 12 9 9 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 803 1530 1582 1857 Size: 803 727 52 275 Fragments arranged by size: 803 727 275 52 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 84 139 1372 1857 Size: 84 55 1233 485 Fragments arranged by size: 1233 485 84 55 BsiEI CG_ry'CG Cuts at: 0 1440 1857 Size: 1440 417 BsiHKAI G_wGCw'C Cuts at: 0 23 110 1183 1292 1857 Size: 23 87 1073 109 565 Fragments arranged by size: 1073 565 109 87 23 BsmI GAATG_Cn' Cuts at: 0 29 1857 Size: 29 1828 Bsp1286I G_dGCh'C Cuts at: 0 23 110 1099 1183 1292 1484 1582 1814 Size: 23 87 989 84 109 192 98 232 Cuts at: 1814 1857 Size: 43 Fragments arranged by size: 989 232 192 109 98 87 84 43 23 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 956 1394 1857 Size: 956 438 463 Fragments arranged by size: 956 463 438 BsrBI CCG'CTC Cuts at: 0 146 1251 1644 1857 Size: 146 1105 393 213 Fragments arranged by size: 1105 393 213 146 BsrDI GCAATG_nn' Cuts at: 0 312 1857 Size: 312 1545 BsrFI r'CCGG_y Cuts at: 0 193 1789 1798 1816 1857 Size: 193 1596 9 18 41 Fragments arranged by size: 1596 193 41 18 9 BssHII G'CGCG_C Cuts at: 0 1680 1682 1857 Size: 1680 2 175 Fragments arranged by size: 1680 175 2 BstAPI GCAn_nnn'nTGC Cuts at: 0 1187 1271 1857 Size: 1187 84 586 Fragments arranged by size: 1187 586 84 BstXI CCAn_nnnn'nTGG Cuts at: 0 339 776 1026 1857 Size: 339 437 250 831 Fragments arranged by size: 831 437 339 250 BstYI r'GATC_y Cuts at: 0 344 840 1857 Size: 344 496 1017 Fragments arranged by size: 1017 496 344 Bsu36I CC'TnA_GG Cuts at: 0 245 1857 Size: 245 1612 BtgI C'CryG_G Cuts at: 0 821 1138 1857 Size: 821 317 719 Fragments arranged by size: 821 719 317 BtsI GCAGTG_nn' Cuts at: 0 57 1099 1269 1857 Size: 57 1042 170 588 Fragments arranged by size: 1042 588 170 57 EaeI y'GGCC_r Cuts at: 0 2 648 778 1437 1545 1733 1796 1857 Size: 2 646 130 659 108 188 63 61 Fragments arranged by size: 659 646 188 130 108 63 61 2 EagI C'GGCC_G Cuts at: 0 1437 1857 Size: 1437 420 EarI CTCTTCn'nnn_ Cuts at: 0 455 470 1042 1055 1480 1857 Size: 455 15 572 13 425 377 Fragments arranged by size: 572 455 425 377 15 13 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 994 1857 Size: 994 863 EcoICRI GAG'CTC Cuts at: 0 21 1857 Size: 21 1836 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 286 361 391 1093 1660 1711 1857 Size: 286 75 30 702 567 51 146 Fragments arranged by size: 702 567 286 146 75 51 30 EcoO109I rG'GnC_Cy Cuts at: 0 57 958 967 1151 1579 1713 1857 Size: 57 901 9 184 428 134 144 Fragments arranged by size: 901 428 184 144 134 57 9 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1527 1857 Size: 1527 330 FseI GG_CCGG'CC Cuts at: 0 1802 1857 Size: 1802 55 FspI TGC'GCA Cuts at: 0 1318 1857 Size: 1318 539 FspAI rTGC'GCAy Cuts at: 0 1318 1857 Size: 1318 539 HaeII r_GCGC'y Cuts at: 0 1407 1612 1622 1857 Size: 1407 205 10 235 Fragments arranged by size: 1407 235 205 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 564 596 1857 Size: 564 32 1261 Fragments arranged by size: 1261 564 32 HindIII A'AGCT_T Cuts at: 0 181 1857 Size: 181 1676 KasI G'GCGC_C Cuts at: 0 1618 1857 Size: 1618 239 MscI TGG'CCA Cuts at: 0 4 650 780 1857 Size: 4 646 130 1077 Fragments arranged by size: 1077 646 130 4 MslI CAynn'nnrTG Cuts at: 0 50 1176 1330 1857 Size: 50 1126 154 527 Fragments arranged by size: 1126 527 154 50 MspA1I CmG'CkG Cuts at: 0 301 551 807 1140 1564 1689 1770 1857 Size: 301 250 256 333 424 125 81 87 Fragments arranged by size: 424 333 301 256 250 125 87 81 NaeI GCC'GGC Cuts at: 0 195 1791 1800 1818 1857 Size: 195 1596 9 18 39 Fragments arranged by size: 1596 195 39 18 9 NarI GG'CG_CC Cuts at: 0 1619 1857 Size: 1619 238 NcoI C'CATG_G Cuts at: 0 821 1857 Size: 821 1036 NgoMIV G'CCGG_C Cuts at: 0 193 1789 1798 1816 1857 Size: 193 1596 9 18 41 Fragments arranged by size: 1596 193 41 18 9 NotI GC'GGCC_GC Cuts at: 0 1437 1857 Size: 1437 420 NspI r_CATG'y Cuts at: 0 939 1179 1233 1317 1857 Size: 939 240 54 84 540 Fragments arranged by size: 939 540 240 84 54 PasI CC'CwG_GG Cuts at: 0 611 1807 1857 Size: 611 1196 50 Fragments arranged by size: 1196 611 50 PciI A'CATG_T Cuts at: 0 935 1175 1857 Size: 935 240 682 Fragments arranged by size: 935 682 240 PfoI T'CCnGG_A Cuts at: 0 263 665 1857 Size: 263 402 1192 Fragments arranged by size: 1192 402 263 PmlI CAC'GTG Cuts at: 0 1830 1857 Size: 1830 27 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1633 1665 1857 Size: 1633 32 192 Fragments arranged by size: 1633 192 32 PpuMI rG'GwC_Cy Cuts at: 0 57 967 1151 1857 Size: 57 910 184 706 Fragments arranged by size: 910 706 184 57 PspOMI G'GGCC_C Cuts at: 0 1480 1578 1857 Size: 1480 98 279 Fragments arranged by size: 1480 279 98 PstI C_TGCA'G Cuts at: 0 166 1857 Size: 166 1691 PvuII CAG'CTG Cuts at: 0 551 807 1564 1689 1857 Size: 551 256 757 125 168 Fragments arranged by size: 757 551 256 168 125 SacI G_AGCT'C Cuts at: 0 23 1857 Size: 23 1834 SacII CC_GC'GG Cuts at: 0 1141 1857 Size: 1141 716 SanDI GG'GwC_CC Cuts at: 0 967 1857 Size: 967 890 SapI GCTCTTCn'nnn_ Cuts at: 0 1055 1480 1857 Size: 1055 425 377 Fragments arranged by size: 1055 425 377 ScaI AGT'ACT Cuts at: 0 172 1857 Size: 172 1685 SfcI C'TryA_G Cuts at: 0 162 1857 Size: 162 1695 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1799 1857 Size: 1799 58 SfoI GGC'GCC Cuts at: 0 1620 1857 Size: 1620 237 SmaI CCC'GGG Cuts at: 0 1577 1857 Size: 1577 280 SmlI C'TyrA_G Cuts at: 0 1303 1857 Size: 1303 554 StuI AGG'CCT Cuts at: 0 595 1034 1084 1857 Size: 595 439 50 773 Fragments arranged by size: 773 595 439 50 StyI C'CwwG_G Cuts at: 0 710 821 1079 1613 1780 1857 Size: 710 111 258 534 167 77 Fragments arranged by size: 710 534 258 167 111 77 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1036 1857 Size: 1036 821 TatI w'GTAC_w Cuts at: 0 170 1857 Size: 170 1687 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 334 366 1819 1851 1857 Size: 334 32 1453 32 6 Fragments arranged by size: 1453 334 32 32 6 Tth111I GACn'n_nGTC Cuts at: 0 235 1857 Size: 235 1622 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1599 1857 Size: 1599 258 XmaI C'CCGG_G Cuts at: 0 1575 1857 Size: 1575 282 XmnI GAAnn'nnTTC Cuts at: 0 184 1672 1857 Size: 184 1488 185 Fragments arranged by size: 1488 185 184 Enzymes that do cut: AarI AcuI AflIII AleI AlfI AloI ApaI ApaLI AvaI BanI BanII BbeI BcgI BglI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstXI BstYI Bsu36I BtgI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FalI FseI FspI FspAI HaeII Hin4I HindIII KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI PciI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI SacII SanDI SapI ScaI SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AlwNI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BglII BlpI BmrI BmtI BplI BsaBI BsaWI BsiWI BsmBI BspEI BspHI BsrGI BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EcoNI EcoRI EcoRV HincII HpaI KpnI MfeI MluI MmeI NdeI NheI NruI NsiI PacI PflMI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SalI SbfI SexAI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI ZraI