################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI AocI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII BbsI BbvCI BciVI BclI BfrI BglII BpuAI BsaBI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I Eco81I EcoO65I EcoRI FbaI FseI FspI HincII HindII HindIII HpaI KpnI MamI MfeI MluI MroI MunI NcoI NdeI NheI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp509I Tth111I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### Alw44I 1476 GTGCAC G^TGCAC AlwNI 1401 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 1476 GTGCAC G^TGCAC Asp700I 1032 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1476 GTGCAC G[AT]GC[AT]^C AvaI 433 CCCGGG C^[CT]CG[GA]G AvrII 1063 CCTAGG C^CTAGG BalI 534 TGGCCA TGG^CCA BcgI 1220 GCAGCAAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 1063 CCTAGG C^CTAGG BpmI 1655 CTGGAG CTGGAG(16/14) BsiHKAI 1476 GTGCAC G[AT]GC[AT]^C BsoBI 433 CCCGGG C^[CT]CG[GA]G BspLU11I 599 ACATGT A^CATGT BsrDI 1134 CATTGC GCAATG(2/0) BstAPI 648 GCACTCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraII 725 GGGACCT [GA]G^G[ACGT]CC[CT] EcoO109I 725 GGGACCT [GA]G^G[ACGT]CC[CT] EcoRV 1057 GATATC GAT^ATC HgaI 1195 GACGC GACGC(5/10) HphI 1445 TCACC GGTGA(8/7) MluNI 534 TGGCCA TGG^CCA MscI 534 TGGCCA TGG^CCA MslI 1567 CACTCTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NruI 735 TCGCGA TCG^CGA NspI 599 ACATGT [GA]CATG^[CT] PpuMI 725 GGGACCT [GA]G^G[AT]CC[CT] ScaI 757 AGTACT AGT^ACT SmaI 433 CCCGGG CCC^GGG SmlI 103 CTCAAG C^T[CT][GA]AG XmaCI 433 CCCGGG C^CCGGG XmaI 433 CCCGGG C^CCGGG XmnI 1032 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AflIII 599 ACATGT A^C[GA][CT]GT 1472 ACGTGT Aor51HI 679 AGCGCT AGC^GCT 1247 AGCGCT ApaI 436 GGGCCC GGGCC^C 1309 GGGCCC BbrPI 1471 CACGTG CAC^GTG 1555 CACGTG BglI 307 GCCGCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 711 GCCGGAGTGGC BlpI 184 GCTGAGC GC^T[ACGT]AGC 1713 GCTGAGC Bpu1102I 184 GCTGAGC GC^T[ACGT]AGC 1713 GCTGAGC BsaAI 1471 CACGTG [CT]AC^GT[GA] 1555 CACGTG BsiEI 296 CGGCCG CG[GA][CT]^CG 686 CGGCCG BspMI 56 ACCTGC ACCTGC(4/8) 728 ACCTGC BsrBI 100 CCGCTC CCGCTC(-3/-3) 559 CCGCTC BssHII 134 GCGCGC G^CGCGC 180 GCGCGC CelII 184 GCTGAGC GC^T[ACGT]AGC 1713 GCTGAGC DsaI 119 CCGCGG C^C[GA][CT]GG 211 CCGCGG EagI 296 CGGCCG C^GGCCG 686 CGGCCG EclXI 296 CGGCCG C^GGCCG 686 CGGCCG Eco47III 679 AGCGCT AGC^GCT 1247 AGCGCT Eco52I 296 CGGCCG C^GGCCG 686 CGGCCG EcoNI 82 CCTCACGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 826 CCTTTCCAAGG EcoT22I 634 ATGCAT ATGCA^T 1131 ATGCAT KspI 119 CCGCGG CCGC^GG 211 CCGCGG NaeI 246 GCCGGC GCC^GGC 659 GCCGGC NgoMIV 246 GCCGGC G^CCGGC 659 GCCGGC NotI 295 GCGGCCGC GC^GGCCGC 685 GCGGCCGC NsiI 634 ATGCAT ATGCA^T 1131 ATGCAT PleI 1269 GACTC GAGTC(4/5) 1355 GAGTC PmaCI 1471 CACGTG CAC^GTG 1555 CACGTG PmlI 1471 CACGTG CAC^GTG 1555 CACGTG PvuII 343 CAGCTG CAG^CTG 1711 CAGCTG SacII 119 CCGCGG CCGC^GG 211 CCGCGG ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AvaII BamHI BanI BanII BbeI BbvI BcnI BfaI BmyI BsaHI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI StyI TaqI TfiI Tru9I TseI Tsp45I TspRI TthHB8I XhoII