(Circular) (Six-base) MAPSORT of: pFN21AE1260.seq Check: 3368 from: 1 to: 6490 pFN21AE1260 6490 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4300 278 Size: 53 83 186 936 2764 2468 Fragments arranged by size: 2764 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3833 1937 Size: 1896 4594 Acc65I G'GTAC_C Cuts at: 3811 3811 Size: 6490 AclI AA'CG_TT Cuts at: 1563 4617 4990 1563 Size: 3054 373 3063 Fragments arranged by size: 3063 3054 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2030 2138 3528 4553 5937 6205 1632 Size: 398 108 1390 1025 1384 268 1917 Fragments arranged by size: 1917 1390 1384 1025 398 268 108 AfeI AGC'GCT Cuts at: 2686 3254 2686 Size: 568 5922 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6293 AflIII A'CryG_T Cuts at: 1203 2604 3477 5389 6366 1203 Size: 1401 873 1912 977 1327 Fragments arranged by size: 1912 1401 1327 977 873 AgeI A'CCGG_T Cuts at: 5321 5321 Size: 6490 AhdI GACnn_n'nnGTC Cuts at: 5219 5219 Size: 6490 AleI CACnn'nnGTG Cuts at: 1166 3576 1166 Size: 2410 4080 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3013 1184 Size: 1829 4661 AlwNI CAG_nnn'CTG Cuts at: 3411 4233 5805 3411 Size: 822 1572 4096 Fragments arranged by size: 4096 1572 822 ApaI G_GGCC'C Cuts at: 2445 3318 2445 Size: 873 5617 ApaLI G'TGCA_C Cuts at: 3481 4547 5703 3481 Size: 1066 1156 4268 Fragments arranged by size: 4268 1156 1066 ApoI r'AATT_y Cuts at: 1493 3799 4025 4177 6286 1493 Size: 2306 226 152 2109 1697 Fragments arranged by size: 2306 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5044 160 Size: 872 4012 1606 Fragments arranged by size: 4012 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6490 AvaI C'yCGr_G Cuts at: 1870 1943 2438 3815 1870 Size: 73 495 1377 4545 Fragments arranged by size: 4545 1377 495 73 AvrII C'CTAG_G Cuts at: 3068 3068 Size: 6490 BamHI G'GATC_C Cuts at: 2805 3123 3459 3820 4187 2805 Size: 318 336 361 367 5108 Fragments arranged by size: 5108 367 361 336 318 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2120 2423 2489 2675 2816 Size: 325 217 627 333 303 66 186 141 Cuts at: 2816 2873 3074 3811 5266 618 Size: 57 201 737 1455 1842 Fragments arranged by size: 1842 1455 737 627 333 325 303 217 201 186 141 66 57 BanII G_rGCy'C Cuts at: 1391 2445 3318 3770 3809 1391 Size: 1054 873 452 39 4072 Fragments arranged by size: 4072 1054 873 452 39 BbeI G_GCGC'C Cuts at: 2124 2427 2493 2877 2124 Size: 303 66 384 5737 Fragments arranged by size: 5737 384 303 66 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5519 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3214 3248 4681 4715 1903 Size: 34 50 34 1193 34 1433 34 3678 Fragments arranged by size: 3678 1433 1193 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4382 5592 4382 Size: 1210 5280 BclI T'GATC_A Cuts at: 1548 1548 Size: 6490 BfrBI ATG'CAT Cuts at: 2641 3138 2641 Size: 497 5993 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2318 2722 5101 Size: 107 122 71 631 605 646 404 2379 Cuts at: 5101 136 Size: 1525 Fragments arranged by size: 2379 1525 646 631 605 404 122 107 71 BglII A'GATC_T Cuts at: 6485 6485 Size: 6490 BlpI GC'TnA_GC Cuts at: 2190 3719 3907 2190 Size: 1529 188 4773 Fragments arranged by size: 4773 1529 188 Bme1580I G_kGCm'C Cuts at: 1790 2445 2636 2678 2772 3077 3318 3485 4551 Size: 655 191 42 94 305 241 167 1066 Cuts at: 4551 5707 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1066 655 305 241 191 167 94 42 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6490 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3520 4221 5179 Size: 416 53 83 399 24 2103 701 958 Cuts at: 5179 442 Size: 1753 Fragments arranged by size: 2103 1753 958 701 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6490 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3681 5150 1239 Size: 2442 1469 2579 Fragments arranged by size: 2579 2442 1469 Bpu10I CC'TnA_GC Cuts at: 2135 2135 Size: 6490 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2093 4615 5480 5778 2093 Size: 2522 865 298 2805 Fragments arranged by size: 2805 2522 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2013 2135 2330 5153 882 Size: 617 514 122 195 2823 2219 Fragments arranged by size: 2823 2219 617 514 195 122 BsaAI yAC'GTr Cuts at: 493 1204 3478 3562 4281 493 Size: 711 2274 84 719 2702 Fragments arranged by size: 2702 2274 719 711 84 BsaBI GATnn'nnATC Cuts at: 6484 6484 Size: 6490 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2121 2424 2490 2874 Size: 53 83 186 936 588 303 66 384 Cuts at: 2874 3201 4297 4679 5314 275 Size: 327 1096 382 635 1451 Fragments arranged by size: 1451 1096 936 635 588 384 382 327 303 186 83 66 53 BsaWI w'CCGG_w Cuts at: 4923 5321 5595 5742 6153 4923 Size: 398 274 147 411 5260 Fragments arranged by size: 5260 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6265 Fragments arranged by size: 6265 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2224 2266 2275 2531 3709 1186 Size: 1038 42 9 256 1178 3967 Fragments arranged by size: 3967 1178 1038 256 42 9 BseYI C'CCAG_C Cuts at: 2053 3246 3453 3537 3714 5693 6189 2053 Size: 1193 207 84 177 1979 496 2354 Fragments arranged by size: 2354 1979 1193 496 207 177 84 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2636 2740 2885 3211 3301 1163 Size: 573 900 104 145 326 90 4352 Fragments arranged by size: 4352 900 573 326 145 104 90 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2304 2694 3940 4701 5729 1540 Size: 275 186 303 390 1246 761 1028 2301 Fragments arranged by size: 2301 1246 1028 761 390 303 275 186 BsiHKAI G_wGCw'C Cuts at: 3485 4551 4636 5707 3485 Size: 1066 85 1071 4268 Fragments arranged by size: 4268 1071 1066 85 BsmI GAATG_Cn' Cuts at: 4001 4094 4001 Size: 93 6397 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2445 2636 2678 2772 3077 3318 3485 Size: 399 655 191 42 94 305 241 167 Cuts at: 3485 3770 3809 4551 4636 5707 1391 Size: 285 39 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 655 399 305 285 241 191 167 94 85 42 39 BspEI T'CCGG_A Cuts at: 6153 6153 Size: 6490 BspHI T'CATG_A Cuts at: 4379 6109 4379 Size: 1730 4760 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2070 2742 3845 844 Size: 1226 672 1103 3489 Fragments arranged by size: 3489 1226 1103 672 BsrBI CCG'CTC Cuts at: 1394 1823 2107 2566 4377 1394 Size: 429 284 459 1811 3507 Fragments arranged by size: 3507 1811 459 429 284 BsrDI GCAATG_nn' Cuts at: 65 1248 3138 4985 5159 65 Size: 1183 1890 1847 174 1396 Fragments arranged by size: 1890 1847 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2251 2338 2353 2664 3582 5134 5321 Size: 110 441 87 15 311 918 1552 187 Cuts at: 5321 1700 Size: 2869 Fragments arranged by size: 2869 1552 918 441 311 187 110 87 15 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6490 BssHII G'CGCG_C Cuts at: 1925 2139 2185 1925 Size: 214 46 6230 Fragments arranged by size: 6230 214 46 BssSI C'ACGA_G Cuts at: 4550 5562 4550 Size: 1012 5478 BstAPI GCAn_nnn'nTGC Cuts at: 794 2659 794 Size: 1865 4625 BstBI TT'CG_AA Cuts at: 5294 5294 Size: 6490 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6490 BstYI r'GATC_y Cuts at: 1972 2805 3123 3459 3820 4187 4572 4589 6041 Size: 833 318 336 361 367 385 17 1452 Cuts at: 6041 6127 6139 6485 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 833 385 367 361 346 336 318 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2124 2216 513 Size: 552 1059 92 4787 Fragments arranged by size: 4787 1059 552 92 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5751 Fragments arranged by size: 5751 683 56 BtsI GCAGTG_nn' Cuts at: 2648 2995 3440 3517 4006 4800 4820 2648 Size: 347 445 77 489 794 20 4318 Fragments arranged by size: 4318 794 489 445 347 77 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6490 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2437 2472 3765 3800 529 Size: 35 1873 35 1293 35 3219 Fragments arranged by size: 3219 1873 1293 35 35 35 DraI TTT'AAA Cuts at: 3794 3794 Size: 6490 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5497 809 Size: 507 4181 1802 Fragments arranged by size: 4181 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2301 2539 2691 Size: 54 1425 50 275 18 471 238 152 Cuts at: 2691 3514 3937 4826 6455 8 Size: 823 423 889 1629 43 Fragments arranged by size: 1629 1425 889 823 471 423 275 238 152 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2301 2691 3937 1537 Size: 275 489 390 1246 4090 Fragments arranged by size: 4090 1246 489 390 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2087 2157 2266 3303 4420 1353 Size: 734 70 109 1037 1117 3423 Fragments arranged by size: 3423 1117 1037 734 109 70 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2187 2227 2260 2269 5077 5451 Size: 1064 138 598 40 33 9 2808 374 Cuts at: 5451 5597 387 Size: 146 1280 Fragments arranged by size: 2808 1280 1064 598 374 146 138 40 33 9 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2030 2138 3528 3681 4553 5150 5937 Size: 393 398 108 1390 153 872 597 787 Cuts at: 5937 6205 1239 Size: 268 1524 Fragments arranged by size: 1524 1390 872 787 597 398 393 268 153 108 EcoNI CCTnn'n_nnAGG Cuts at: 2091 2835 2091 Size: 744 5746 EcoO109I rG'GnC_Cy Cuts at: 2731 2731 Size: 6490 EcoRI G'AATT_C Cuts at: 3799 4177 3799 Size: 378 6112 EcoRV GAT'ATC Cuts at: 3064 3064 Size: 6490 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2861 3393 2861 Size: 532 5958 FspI TGC'GCA Cuts at: 4996 4996 Size: 6490 HaeII r_GCGC'y Cuts at: 1678 2124 2427 2493 2688 2877 3256 5637 1678 Size: 446 303 66 195 189 379 2381 2531 Fragments arranged by size: 2531 2381 446 379 303 195 189 66 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5131 5163 5205 5237 1373 Size: 32 135 32 3559 32 42 32 2626 Fragments arranged by size: 3559 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3834 4080 669 Size: 988 281 1896 246 3079 Fragments arranged by size: 3079 1896 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6490 HpaI GTT'AAC Cuts at: 4080 4080 Size: 6490 KasI G'GCGC_C Cuts at: 2120 2423 2489 2873 2120 Size: 303 66 384 5737 Fragments arranged by size: 5737 384 303 66 KpnI G_GTAC'C Cuts at: 3815 3815 Size: 6490 MfeI C'AATT_G Cuts at: 4089 4089 Size: 6490 MluI A'CGCG_T Cuts at: 6366 6366 Size: 6490 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5604 5788 1305 Size: 4299 184 2007 Fragments arranged by size: 4299 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2541 10 Size: 54 1425 343 709 3959 Fragments arranged by size: 3959 1425 709 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3576 4448 4807 4966 518 Size: 648 2410 872 359 159 2042 Fragments arranged by size: 2410 2042 872 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2118 2126 2218 2306 2350 2378 2398 2505 Size: 512 8 92 88 44 28 20 107 Cuts at: 2505 3457 3718 3733 3906 4583 5731 5976 1606 Size: 952 261 15 173 677 1148 245 2120 Fragments arranged by size: 2120 1148 952 677 512 261 245 173 107 92 88 44 28 20 15 8 NaeI GCC'GGC Cuts at: 2253 2666 2253 Size: 413 6077 NarI GG'CG_CC Cuts at: 2121 2424 2490 2874 2121 Size: 303 66 384 5737 Fragments arranged by size: 5737 384 303 66 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5938 NdeI CA'TA_TG Cuts at: 387 387 Size: 6490 NgoMIV G'CCGG_C Cuts at: 2251 2664 2251 Size: 413 6077 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6490 NotI GC'GGCC_GC Cuts at: 2301 2691 3937 2301 Size: 390 1246 4854 Fragments arranged by size: 4854 1246 390 NruI TCG'CGA Cuts at: 2742 2742 Size: 6490 NsiI A_TGCA'T Cuts at: 2643 3140 2643 Size: 497 5993 NspI r_CATG'y Cuts at: 1132 1229 2608 3848 5393 1132 Size: 97 1379 1240 1545 2229 Fragments arranged by size: 2229 1545 1379 1240 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6490 PciI A'CATG_T Cuts at: 2604 5389 2604 Size: 2785 3705 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6490 PmeI GTTT'AAAC Cuts at: 3794 3794 Size: 6490 PmlI CAC'GTG Cuts at: 3478 3562 3478 Size: 84 6406 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2403 2435 4559 4591 6072 6104 2403 Size: 32 2124 32 1481 32 2789 Fragments arranged by size: 2789 2124 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2731 2731 Size: 6490 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6490 PsiI TTA'TAA Cuts at: 4060 4060 Size: 6490 PspOMI G'GGCC_C Cuts at: 2441 3314 2441 Size: 873 5617 PstI C_TGCA'G Cuts at: 830 2067 2510 3210 3842 830 Size: 1237 443 700 632 3478 Fragments arranged by size: 3478 1237 700 632 443 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6490 PvuII CAG'CTG Cuts at: 2350 3718 2350 Size: 1368 5122 SacII CC_GC'GG Cuts at: 2127 2219 2127 Size: 92 6398 SalI G'TCGA_C Cuts at: 1936 3832 1936 Size: 1896 4594 SbfI CC_TGCA'GG Cuts at: 3842 3842 Size: 6490 ScaI AGT'ACT Cuts at: 2764 4738 6450 2764 Size: 1974 1712 2804 Fragments arranged by size: 2804 1974 1712 SfcI C'TryA_G Cuts at: 826 1045 2063 2506 2927 3206 3838 4973 5654 Size: 219 1018 443 421 279 632 1135 681 Cuts at: 5654 5845 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1018 681 632 443 421 279 219 191 SfoI GGC'GCC Cuts at: 2122 2425 2491 2875 2122 Size: 303 66 384 5737 Fragments arranged by size: 5737 384 303 66 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6490 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6490 SmaI CCC'GGG Cuts at: 1872 2440 3817 1872 Size: 568 1377 4545 Fragments arranged by size: 4545 1377 568 SmlI C'TyrA_G Cuts at: 820 1017 1943 2108 4594 5495 5757 820 Size: 197 926 165 2486 901 262 1553 Fragments arranged by size: 2486 1553 926 901 262 197 165 SnaBI TAC'GTA Cuts at: 493 4281 493 Size: 3788 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6490 SphI G_CATG'C Cuts at: 1132 3848 1132 Size: 2716 3774 SspI AAT'ATT Cuts at: 5 52 4414 5 Size: 47 4362 2081 Fragments arranged by size: 4362 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6490 StyI C'CwwG_G Cuts at: 513 1065 2406 2836 3068 3929 513 Size: 552 1341 430 232 861 3074 Fragments arranged by size: 3074 1341 861 552 430 232 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3260 3467 3728 4517 4534 4687 4872 Size: 1255 1680 207 261 789 17 153 185 Cuts at: 4872 5317 325 Size: 445 1498 Fragments arranged by size: 1680 1498 1255 789 445 261 207 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2762 3027 4736 Size: 274 80 33 51 1443 785 265 1709 Cuts at: 4736 6448 96 Size: 1712 138 Fragments arranged by size: 1712 1709 1443 785 274 265 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3173 3205 6450 6482 3173 Size: 32 3245 32 3181 Fragments arranged by size: 3245 3181 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6490 XbaI T'CTAG_A Cuts at: 3826 3826 Size: 6490 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6490 XmaI C'CCGG_G Cuts at: 1870 2438 3815 1870 Size: 568 1377 4545 Fragments arranged by size: 4545 1377 568 XmnI GAAnn'nnTTC Cuts at: 3041 4619 3041 Size: 1578 4912 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4298 276 Size: 53 83 186 936 2764 2468 Fragments arranged by size: 2764 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI PacI PfoI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI