(Linear) (Six-base) MAPSORT of: KIEE1260.seq Check: 6856 from: 1 to: 1785 KIEE1260.orf With 182 enzymes: SgfI * July 3, 2012 16:50 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 25 133 1523 1785 Size: 25 108 1390 262 Fragments arranged by size: 1390 262 108 25 AfeI AGC'GCT Cuts at: 0 681 1249 1785 Size: 681 568 536 Fragments arranged by size: 681 568 536 AflIII A'CryG_T Cuts at: 0 599 1472 1785 Size: 599 873 313 Fragments arranged by size: 873 599 313 AleI CACnn'nnGTG Cuts at: 0 1571 1785 Size: 1571 214 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1008 1785 Size: 1008 777 AlwNI CAG_nnn'CTG Cuts at: 0 1406 1785 Size: 1406 379 ApaI G_GGCC'C Cuts at: 0 440 1313 1785 Size: 440 873 472 Fragments arranged by size: 873 472 440 ApaLI G'TGCA_C Cuts at: 0 1476 1785 Size: 1476 309 AvaI C'yCGr_G Cuts at: 0 433 1785 Size: 433 1352 AvrII C'CTAG_G Cuts at: 0 1063 1785 Size: 1063 722 BamHI G'GATC_C Cuts at: 0 800 1118 1454 1785 Size: 800 318 336 331 Fragments arranged by size: 800 336 331 318 BanI G'GyrC_C Cuts at: 0 115 418 484 670 811 868 1069 1785 Size: 115 303 66 186 141 57 201 716 Fragments arranged by size: 716 303 201 186 141 115 66 57 BanII G_rGCy'C Cuts at: 0 440 1313 1765 1785 Size: 440 873 452 20 Fragments arranged by size: 873 452 440 20 BbeI G_GCGC'C Cuts at: 0 119 422 488 872 1785 Size: 119 303 66 384 913 Fragments arranged by size: 913 384 303 119 66 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1209 1243 1785 Size: 1209 34 542 Fragments arranged by size: 1209 542 34 BfrBI ATG'CAT Cuts at: 0 636 1133 1785 Size: 636 497 652 Fragments arranged by size: 652 636 497 BglI GCCn_nnn'nGGC Cuts at: 0 313 717 1785 Size: 313 404 1068 Fragments arranged by size: 1068 404 313 BlpI GC'TnA_GC Cuts at: 0 185 1714 1785 Size: 185 1529 71 Fragments arranged by size: 1529 185 71 Bme1580I G_kGCm'C Cuts at: 0 440 631 673 767 1072 1313 1480 1785 Size: 440 191 42 94 305 241 167 305 Fragments arranged by size: 440 305 305 241 191 167 94 42 BmrI ACTGGGnnnn_n' Cuts at: 0 1515 1785 Size: 1515 270 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1676 1785 Size: 1676 109 Bpu10I CC'TnA_GC Cuts at: 0 130 1785 Size: 130 1655 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 88 1785 Size: 88 1697 BsaI GGTCTCn'nnnn_ Cuts at: 0 8 130 325 1785 Size: 8 122 195 1460 Fragments arranged by size: 1460 195 122 8 BsaAI yAC'GTr Cuts at: 0 1473 1557 1785 Size: 1473 84 228 Fragments arranged by size: 1473 228 84 BsaHI Gr'CG_yC Cuts at: 0 116 419 485 869 1196 1785 Size: 116 303 66 384 327 589 Fragments arranged by size: 589 384 327 303 116 66 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 219 261 270 526 1704 1785 Size: 219 42 9 256 1178 81 Fragments arranged by size: 1178 256 219 81 42 9 BseYI C'CCAG_C Cuts at: 0 48 1241 1448 1532 1709 1785 Size: 48 1193 207 84 177 76 Fragments arranged by size: 1193 207 177 84 76 48 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 631 735 880 1206 1296 1785 Size: 631 104 145 326 90 489 Fragments arranged by size: 631 489 326 145 104 90 BsiEI CG_ry'CG Cuts at: 0 299 689 1785 Size: 299 390 1096 Fragments arranged by size: 1096 390 299 BsiHKAI G_wGCw'C Cuts at: 0 1480 1785 Size: 1480 305 Bsp1286I G_dGCh'C Cuts at: 0 440 631 673 767 1072 1313 1480 1765 Size: 440 191 42 94 305 241 167 285 Cuts at: 1765 1785 Size: 20 Fragments arranged by size: 440 305 285 241 191 167 94 42 20 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 65 737 1785 Size: 65 672 1048 Fragments arranged by size: 1048 672 65 BsrBI CCG'CTC Cuts at: 0 102 561 1785 Size: 102 459 1224 Fragments arranged by size: 1224 459 102 BsrDI GCAATG_nn' Cuts at: 0 1133 1785 Size: 1133 652 BsrFI r'CCGG_y Cuts at: 0 246 333 348 659 1577 1785 Size: 246 87 15 311 918 208 Fragments arranged by size: 918 311 246 208 87 15 BssHII G'CGCG_C Cuts at: 0 134 180 1785 Size: 134 46 1605 Fragments arranged by size: 1605 134 46 BstAPI GCAn_nnn'nTGC Cuts at: 0 654 1785 Size: 654 1131 BstYI r'GATC_y Cuts at: 0 800 1118 1454 1785 Size: 800 318 336 331 Fragments arranged by size: 800 336 331 318 BtgI C'CryG_G Cuts at: 0 119 211 1785 Size: 119 92 1574 Fragments arranged by size: 1574 119 92 BtsI GCAGTG_nn' Cuts at: 0 643 990 1435 1512 1785 Size: 643 347 445 77 273 Fragments arranged by size: 643 445 347 273 77 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 432 467 1760 1785 Size: 432 35 1293 25 Fragments arranged by size: 1293 432 35 25 EaeI y'GGCC_r Cuts at: 0 296 534 686 1509 1785 Size: 296 238 152 823 276 Fragments arranged by size: 823 296 276 238 152 EagI C'GGCC_G Cuts at: 0 296 686 1785 Size: 296 390 1099 Fragments arranged by size: 1099 390 296 EarI CTCTTCn'nnn_ Cuts at: 0 82 152 261 1298 1785 Size: 82 70 109 1037 487 Fragments arranged by size: 1037 487 109 82 70 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 182 222 255 264 1785 Size: 182 40 33 9 1521 Fragments arranged by size: 1521 182 40 33 9 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 25 133 1523 1676 1785 Size: 25 108 1390 153 109 Fragments arranged by size: 1390 153 109 108 25 EcoNI CCTnn'n_nnAGG Cuts at: 0 86 830 1785 Size: 86 744 955 Fragments arranged by size: 955 744 86 EcoO109I rG'GnC_Cy Cuts at: 0 726 1785 Size: 726 1059 EcoRV GAT'ATC Cuts at: 0 1059 1785 Size: 1059 726 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 856 1388 1785 Size: 856 532 397 Fragments arranged by size: 856 532 397 HaeII r_GCGC'y Cuts at: 0 119 422 488 683 872 1251 1785 Size: 119 303 66 195 189 379 534 Fragments arranged by size: 534 379 303 195 189 119 66 KasI G'GCGC_C Cuts at: 0 115 418 484 868 1785 Size: 115 303 66 384 917 Fragments arranged by size: 917 384 303 115 66 MscI TGG'CCA Cuts at: 0 536 1785 Size: 536 1249 MslI CAynn'nnrTG Cuts at: 0 1571 1785 Size: 1571 214 MspA1I CmG'CkG Cuts at: 0 113 121 213 301 345 373 393 500 Size: 113 8 92 88 44 28 20 107 Cuts at: 500 1452 1713 1728 1785 Size: 952 261 15 57 Fragments arranged by size: 952 261 113 107 92 88 57 44 28 20 15 8 NaeI GCC'GGC Cuts at: 0 248 661 1785 Size: 248 413 1124 Fragments arranged by size: 1124 413 248 NarI GG'CG_CC Cuts at: 0 116 419 485 869 1785 Size: 116 303 66 384 916 Fragments arranged by size: 916 384 303 116 66 NgoMIV G'CCGG_C Cuts at: 0 246 659 1785 Size: 246 413 1126 Fragments arranged by size: 1126 413 246 NotI GC'GGCC_GC Cuts at: 0 296 686 1785 Size: 296 390 1099 Fragments arranged by size: 1099 390 296 NruI TCG'CGA Cuts at: 0 737 1785 Size: 737 1048 NsiI A_TGCA'T Cuts at: 0 638 1135 1785 Size: 638 497 650 Fragments arranged by size: 650 638 497 NspI r_CATG'y Cuts at: 0 603 1785 Size: 603 1182 PciI A'CATG_T Cuts at: 0 599 1785 Size: 599 1186 PmlI CAC'GTG Cuts at: 0 1473 1557 1785 Size: 1473 84 228 Fragments arranged by size: 1473 228 84 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 398 430 1785 Size: 398 32 1355 Fragments arranged by size: 1355 398 32 PpuMI rG'GwC_Cy Cuts at: 0 726 1785 Size: 726 1059 PspOMI G'GGCC_C Cuts at: 0 436 1309 1785 Size: 436 873 476 Fragments arranged by size: 873 476 436 PstI C_TGCA'G Cuts at: 0 62 505 1205 1785 Size: 62 443 700 580 Fragments arranged by size: 700 580 443 62 PvuII CAG'CTG Cuts at: 0 345 1713 1785 Size: 345 1368 72 Fragments arranged by size: 1368 345 72 SacII CC_GC'GG Cuts at: 0 122 214 1785 Size: 122 92 1571 Fragments arranged by size: 1571 122 92 ScaI AGT'ACT Cuts at: 0 759 1785 Size: 759 1026 SfcI C'TryA_G Cuts at: 0 58 501 922 1201 1785 Size: 58 443 421 279 584 Fragments arranged by size: 584 443 421 279 58 SfoI GGC'GCC Cuts at: 0 117 420 486 870 1785 Size: 117 303 66 384 915 Fragments arranged by size: 915 384 303 117 66 SmaI CCC'GGG Cuts at: 0 435 1785 Size: 435 1350 SmlI C'TyrA_G Cuts at: 0 103 1785 Size: 103 1682 StyI C'CwwG_G Cuts at: 0 401 831 1063 1785 Size: 401 430 232 722 Fragments arranged by size: 722 430 401 232 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1255 1462 1723 1785 Size: 1255 207 261 62 Fragments arranged by size: 1255 261 207 62 TatI w'GTAC_w Cuts at: 0 757 1022 1785 Size: 757 265 763 Fragments arranged by size: 763 757 265 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1168 1200 1785 Size: 1168 32 585 Fragments arranged by size: 1168 585 32 XmaI C'CCGG_G Cuts at: 0 433 1785 Size: 433 1352 XmnI GAAnn'nnTTC Cuts at: 0 1036 1785 Size: 1036 749 Enzymes that do cut: AcuI AfeI AflIII AleI AlfI AlwNI ApaI ApaLI AvaI AvrII BamHI BanI BanII BbeI BcgI BfrBI BglI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstYI BtgI BtsI CspCI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII KasI MscI MslI MspA1I NaeI NarI NgoMIV NotI NruI NsiI NspI PciI PmlI PpiI PpuMI PspOMI PstI PvuII SacII ScaI SfcI SfoI SmaI SmlI StyI TaqII TatI TstI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AgeI AhdI AloI ApoI AscI AseI AsiSI BaeI BbsI BbvCI BciVI BclI BglII BmgBI BmtI BplI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EcoICRI EcoRI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI NcoI NdeI NheI PacI PasI PflMI PfoI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI ZraI