(Circular) (Six-base) MAPSORT of: pFN21AB7690.seq Check: 8460 from: 1 to: 5938 pFN21AB7690 5938 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3748 278 Size: 53 83 186 936 2212 2468 Fragments arranged by size: 2468 2212 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2476 3089 3126 3281 1937 Size: 539 613 37 155 4594 Fragments arranged by size: 4594 613 539 155 37 Acc65I G'GTAC_C Cuts at: 3259 3259 Size: 5938 AclI AA'CG_TT Cuts at: 1563 4065 4438 1563 Size: 2502 373 3063 Fragments arranged by size: 3063 2502 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2553 4001 5385 5653 1632 Size: 921 1448 1384 268 1917 Fragments arranged by size: 1917 1448 1384 921 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5741 AflIII A'CryG_T Cuts at: 1203 4837 5814 1203 Size: 3634 977 1327 Fragments arranged by size: 3634 1327 977 AgeI A'CCGG_T Cuts at: 4769 4769 Size: 5938 AhdI GACnn_n'nnGTC Cuts at: 2650 4667 2650 Size: 2017 3921 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5938 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5938 AlwNI CAG_nnn'CTG Cuts at: 2048 2456 3681 5253 2048 Size: 408 1225 1572 2733 Fragments arranged by size: 2733 1572 1225 408 ApaLI G'TGCA_C Cuts at: 3995 5151 3995 Size: 1156 4782 ApoI r'AATT_y Cuts at: 1493 2619 2777 3247 3473 3625 5734 1493 Size: 1126 158 470 226 152 2109 1697 Fragments arranged by size: 2109 1697 1126 470 226 158 152 AseI AT'TA_AT Cuts at: 160 1032 4492 160 Size: 872 3460 1606 Fragments arranged by size: 3460 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5938 AvaI C'yCGr_G Cuts at: 1870 1943 2137 3263 1870 Size: 73 194 1126 4545 Fragments arranged by size: 4545 1126 194 73 BamHI G'GATC_C Cuts at: 3268 3635 3268 Size: 367 5571 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2638 3259 4714 618 Size: 325 217 627 851 621 1455 1842 Fragments arranged by size: 1842 1455 851 627 621 325 217 BanII G_rGCy'C Cuts at: 1391 3257 1391 Size: 1866 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4967 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4129 4163 1903 Size: 34 2192 34 3678 Fragments arranged by size: 3678 2192 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2078 3830 5040 2078 Size: 1752 1210 2976 Fragments arranged by size: 2976 1752 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5938 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4549 136 Size: 107 122 71 631 605 2877 1525 Fragments arranged by size: 2877 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2320 5933 2320 Size: 3613 2325 BlpI GC'TnA_GC Cuts at: 2937 3355 2937 Size: 418 5520 Bme1580I G_kGCm'C Cuts at: 1790 3999 5155 1790 Size: 2209 1156 2573 Fragments arranged by size: 2573 2209 1156 BmgBI CAC'GTC Cuts at: 1318 3087 1318 Size: 1769 4169 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3669 4627 442 Size: 416 53 83 399 24 2252 958 1753 Fragments arranged by size: 2252 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5938 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2505 2505 Size: 5938 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4598 1239 Size: 3359 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4063 4928 5226 4063 Size: 865 298 4775 Fragments arranged by size: 4775 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4601 882 Size: 617 3102 2219 Fragments arranged by size: 3102 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3729 493 Size: 711 2525 2702 Fragments arranged by size: 2702 2525 711 BsaBI GATnn'nnATC Cuts at: 5932 5932 Size: 5938 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3745 4127 4762 275 Size: 53 83 186 936 2212 382 635 1451 Fragments arranged by size: 2212 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4371 4769 5043 5190 5601 4371 Size: 398 274 147 411 4708 Fragments arranged by size: 4708 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2029 2059 3076 3106 1178 Size: 30 165 30 626 30 1017 30 4010 Fragments arranged by size: 4010 1017 626 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3008 3047 3209 1186 Size: 1822 39 162 3915 Fragments arranged by size: 3915 1822 162 39 BseYI C'CCAG_C Cuts at: 2041 2063 2125 5141 5637 2041 Size: 22 62 3016 496 2342 Fragments arranged by size: 3016 2342 496 62 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2469 1163 Size: 573 733 4632 Fragments arranged by size: 4632 733 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3388 4149 5177 1540 Size: 275 186 1387 761 1028 2301 Fragments arranged by size: 2301 1387 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2144 3999 4084 5155 2144 Size: 1855 85 1071 2927 Fragments arranged by size: 2927 1855 1071 85 BsmI GAATG_Cn' Cuts at: 2520 2679 3449 3542 2520 Size: 159 770 93 4916 Fragments arranged by size: 4916 770 159 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2144 3257 3999 4084 5155 1391 Size: 399 354 1113 742 85 1071 2174 Fragments arranged by size: 2174 1113 1071 742 399 354 85 BspEI T'CCGG_A Cuts at: 5601 5601 Size: 5938 BspHI T'CATG_A Cuts at: 3827 5557 3827 Size: 1730 4208 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2668 2683 3293 844 Size: 1824 15 610 3489 Fragments arranged by size: 3489 1824 610 15 BsrBI CCG'CTC Cuts at: 1394 1823 3825 1394 Size: 429 2002 3507 Fragments arranged by size: 3507 2002 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4433 4607 65 Size: 1183 3185 174 1396 Fragments arranged by size: 3185 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4582 4769 1700 Size: 110 2772 187 2869 Fragments arranged by size: 2869 2772 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5938 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5938 BssSI C'ACGA_G Cuts at: 3998 5010 3998 Size: 1012 4926 BstAPI GCAn_nnn'nTGC Cuts at: 794 2456 794 Size: 1662 4276 BstBI TT'CG_AA Cuts at: 4742 4742 Size: 5938 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5938 BstXI CCAn_nnnn'nTGG Cuts at: 3071 3071 Size: 5938 BstYI r'GATC_y Cuts at: 1972 2320 2536 3208 3268 3635 4020 4037 5489 Size: 348 216 672 60 367 385 17 1452 Cuts at: 5489 5575 5587 5933 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 672 385 367 348 346 216 86 60 17 12 Bsu36I CC'TnA_GG Cuts at: 2964 3096 2964 Size: 132 5806 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5386 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5199 Fragments arranged by size: 5199 683 56 BtsI GCAGTG_nn' Cuts at: 3454 4248 4268 3454 Size: 794 20 5124 Fragments arranged by size: 5124 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5938 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3091 3126 529 Size: 35 2527 35 3341 Fragments arranged by size: 3341 2527 35 35 DraI TTT'AAA Cuts at: 2352 3242 2352 Size: 890 5048 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4945 809 Size: 507 3629 1802 Fragments arranged by size: 3629 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3385 4274 5903 Size: 54 1425 50 275 18 1555 889 1629 Cuts at: 5903 8 Size: 43 Fragments arranged by size: 1629 1555 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3385 1537 Size: 275 1573 4090 Fragments arranged by size: 4090 1573 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2765 3868 1353 Size: 1412 1103 3423 Fragments arranged by size: 3423 1412 1103 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4525 4899 5045 387 Size: 1064 138 2936 374 146 1280 Fragments arranged by size: 2936 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2553 4001 4598 5385 5653 1239 Size: 393 921 1448 597 787 268 1524 Fragments arranged by size: 1524 1448 921 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2224 2224 Size: 5938 EcoO109I rG'GnC_Cy Cuts at: 2680 2680 Size: 5938 EcoRI G'AATT_C Cuts at: 3247 3625 3247 Size: 378 5560 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2037 2753 2037 Size: 716 5222 FspI TGC'GCA Cuts at: 4444 4444 Size: 5938 HaeII r_GCGC'y Cuts at: 1678 5085 1678 Size: 3407 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2636 2668 4579 4611 4653 Size: 32 135 32 1064 32 1911 32 42 Cuts at: 4653 4685 1373 Size: 32 2626 Fragments arranged by size: 2626 1911 1064 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2477 3127 3282 3528 669 Size: 988 281 539 650 155 246 3079 Fragments arranged by size: 3079 988 650 539 281 246 155 HindIII A'AGCT_T Cuts at: 748 748 Size: 5938 HpaI GTT'AAC Cuts at: 3528 3528 Size: 5938 KpnI G_GTAC'C Cuts at: 3263 3263 Size: 5938 MfeI C'AATT_G Cuts at: 3537 3537 Size: 5938 MluI A'CGCG_T Cuts at: 5814 5814 Size: 5938 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2289 5052 5236 1305 Size: 984 2763 184 2007 Fragments arranged by size: 2763 2007 984 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4116 Fragments arranged by size: 4116 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3020 3896 4255 4414 518 Size: 648 1854 876 359 159 2042 Fragments arranged by size: 2042 1854 876 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2212 2864 3354 4031 5179 5424 1606 Size: 606 652 490 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 652 606 490 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5386 NdeI CA'TA_TG Cuts at: 387 387 Size: 5938 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5938 NotI GC'GGCC_GC Cuts at: 3385 3385 Size: 5938 NspI r_CATG'y Cuts at: 1132 1229 2314 3296 4841 1132 Size: 97 1085 982 1545 2229 Fragments arranged by size: 2229 1545 1085 982 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5938 PciI A'CATG_T Cuts at: 4837 4837 Size: 5938 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5938 PfoI T'CCnGG_A Cuts at: 2781 2781 Size: 5938 PmeI GTTT'AAAC Cuts at: 3242 3242 Size: 5938 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4007 4039 5520 5552 4007 Size: 32 1481 32 4393 Fragments arranged by size: 4393 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2680 2680 Size: 5938 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5938 PsiI TTA'TAA Cuts at: 3508 3508 Size: 5938 PstI C_TGCA'G Cuts at: 830 3290 830 Size: 2460 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5938 PvuII CAG'CTG Cuts at: 2864 2864 Size: 5938 SalI G'TCGA_C Cuts at: 1936 2475 3125 3280 1936 Size: 539 650 155 4594 Fragments arranged by size: 4594 650 539 155 SbfI CC_TGCA'GG Cuts at: 3290 3290 Size: 5938 ScaI AGT'ACT Cuts at: 4186 5898 4186 Size: 1712 4226 SfcI C'TryA_G Cuts at: 826 1045 2119 3286 4421 5102 5293 826 Size: 219 1074 1167 1135 681 191 1471 Fragments arranged by size: 1471 1167 1135 1074 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5938 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5938 SmaI CCC'GGG Cuts at: 1872 3265 1872 Size: 1393 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4042 4943 5205 820 Size: 197 926 2099 901 262 1553 Fragments arranged by size: 2099 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3729 493 Size: 3236 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5938 SphI G_CATG'C Cuts at: 1132 3296 1132 Size: 2164 3774 SspI AAT'ATT Cuts at: 5 52 2348 3862 5 Size: 47 2296 1514 2081 Fragments arranged by size: 2296 2081 1514 47 StuI AGG'CCT Cuts at: 1517 2399 1517 Size: 882 5056 StyI C'CwwG_G Cuts at: 513 1065 3377 513 Size: 552 2312 3074 Fragments arranged by size: 3074 2312 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2640 3965 3982 4135 4320 4765 325 Size: 1255 1060 1325 17 153 185 445 1498 Fragments arranged by size: 1498 1325 1255 1060 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2379 4184 5896 Size: 274 80 33 51 1443 402 1805 1712 Cuts at: 5896 96 Size: 138 Fragments arranged by size: 1805 1712 1443 402 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2029 2061 3076 3108 5898 5930 2029 Size: 32 1015 32 2790 32 2037 Fragments arranged by size: 2790 2037 1015 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5938 XbaI T'CTAG_A Cuts at: 3274 3274 Size: 5938 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5938 XmaI C'CCGG_G Cuts at: 1870 3263 1870 Size: 1393 4545 XmnI GAAnn'nnTTC Cuts at: 2739 4067 2739 Size: 1328 4610 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3746 276 Size: 53 83 186 936 2212 2468 Fragments arranged by size: 2468 2212 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI