Restriction map for
pFN21AB6465
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI Alw44I AlwNI Aor51HI
ApaI ApaLI ApoI AscI Asp700I
Asp718I AspHI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BfrI BglI BlnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsiYI BslI BsmBI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstNI BstPI BstUI
BstXI BstZ17I CelII CfoI Cfr10I
Cfr13I CpoI DraII DraIII DrdI
EaeI EagI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRII
EcoRV EcoT22I FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
KspI MaeII MfeI MluI MluNI
MroI MscI MslI MspA1I MunI
MvaI MvnI NaeI NarI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AhdI 499 GACATAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 604 CCTGAGG CC^T[ACGT]AGG
AspEI 499 GACATAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BclI 213 TGATCA T^GATCA
BcnI 87 CCCGG CC^[GC]GG
BfaI 830 CTAG C^TAG
BglII 151 AGATCT A^GATCT
BsaBI 214 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 655 GAGGAG GAGGAG(10/8)
BspDI 729 ATCGAT AT^CGAT
BssKI 87 CCCGG ^CC[ACGT]GG
BstAPI 808 GCATACTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 604 CCTGAGG CC^T[ACGT]AGG
Cac8I 397 GCCAGC GC[ACGT]^[ACGT]GC
ClaI 729 ATCGAT AT^CGAT
DraI 429 TTTAAA TTT^AAA
DsaI 631 CCATGG C^C[GA][CT]GG
Eam1105I 499 GACATAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 886 CTCTTC CTCTTC(1/4)
Eco81I 604 CCTGAGG CC^T[ACGT]AGG
FbaI 213 TGATCA T^GATCA
HapII 88 CCGG C^CGG
HindIII 109 AAGCTT A^AGCTT
HpaII 88 CCGG C^CGG
HphI 355 GGTGA GGTGA(8/7)
Ksp632I 886 CTCTTC CTCTTC(1/4)
MaeI 830 CTAG C^TAG
MamI 214 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 88 CCGG C^CGG
MwoI 808 GCATACTATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 87 CCCGG CC^[GC]GG
NcoI 631 CCATGG C^CATGG
PleI 563 GAGTC GAGTC(4/5)
ScrFI 87 CCCGG CC^[ACGT]GG
Tsp45I 595 GTGAC ^GT[GC]AC
TspRI 575 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 641 CCAAAGAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AfaI 122 GTAC GT^AC
138 GTAC
AlwI 406 GATCC GGATC(4/5)
8 GGATC
BbsI 907 GAAGAC GAAGAC(2/6)
507 GTCTTC
BpuAI 907 GAAGAC GAAGAC(2/6)
507 GTCTTC
BsaJI 178 CCTTGG C^C[ACGT][ACGT]GG
631 CCATGG
BsgI 469 CTGCAC GTGCAG(16/14)
932 CTGCAC
BstYI 151 AGATCT [GA]^GATC[CT]
405 AGATCC
DdeI 257 CTAAG C^T[ACGT]AG
605 CTGAG
EcoT14I 178 CCTTGG C^C[AT][AT]GG
631 CCATGG
MaeIII 595 GTGAC ^GT[ACGT]AC
614 GTTAC
MflI 151 AGATCT [GA]^GATC[CT]
405 AGATCC
NlaIII 493 CATG CATG^
632 CATG
RsaI 122 GTAC GT^AC
138 GTAC
StyI 178 CCTTGG C^C[AT][AT]GG
631 CCATGG
TaqI 222 TCGA T^CGA
730 TCGA
TfiI 732 GATTC G^A[AT]TC
862 GAATC
TthHB8I 222 TCGA T^CGA
730 TCGA
XhoII 151 AGATCT [GA]^GATC[CT]
405 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AluI AseI AsnI BbvI BsmAI
BsrI BstF5I DpnII Fnu4HI FokI
HaeIII HinfI ItaI MboI MboII
MnlI MseI NdeII PshBI Sau3AI
Tru9I TseI Tsp509I