(Circular) (Six-base) MAPSORT of: pFN21AE4094.seq Check: 485 from: 1 to: 6025 pFN21AE4094 6025 bp With 182 enzymes: SgfI * July 6, 2012 17:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3835 278 Size: 53 83 186 936 2299 2468 Fragments arranged by size: 2468 2299 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2747 3368 1937 Size: 810 621 4594 Fragments arranged by size: 4594 810 621 Acc65I G'GTAC_C Cuts at: 3346 3346 Size: 6025 AclI AA'CG_TT Cuts at: 1563 3093 4152 4525 1563 Size: 1530 1059 373 3063 Fragments arranged by size: 3063 1530 1059 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4088 5472 5740 1632 Size: 2456 1384 268 1917 Fragments arranged by size: 2456 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5828 AflIII A'CryG_T Cuts at: 1203 4924 5901 1203 Size: 3721 977 1327 Fragments arranged by size: 3721 1327 977 AgeI A'CCGG_T Cuts at: 4856 4856 Size: 6025 AhdI GACnn_n'nnGTC Cuts at: 4754 4754 Size: 6025 AleI CACnn'nnGTG Cuts at: 1166 2627 3316 1166 Size: 1461 689 3875 Fragments arranged by size: 3875 1461 689 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3053 1184 Size: 1869 4156 AlwNI CAG_nnn'CTG Cuts at: 2041 2521 2735 2981 3768 5340 2041 Size: 480 214 246 787 1572 2726 Fragments arranged by size: 2726 1572 787 480 246 214 ApaLI G'TGCA_C Cuts at: 3132 4082 5238 3132 Size: 950 1156 3919 Fragments arranged by size: 3919 1156 950 ApoI r'AATT_y Cuts at: 1493 2178 2351 3334 3560 3712 5821 1493 Size: 685 173 983 226 152 2109 1697 Fragments arranged by size: 2109 1697 983 685 226 173 152 AseI AT'TA_AT Cuts at: 160 1032 4579 160 Size: 872 3547 1606 Fragments arranged by size: 3547 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6025 AvaI C'yCGr_G Cuts at: 1870 1943 3350 1870 Size: 73 1407 4545 Fragments arranged by size: 4545 1407 73 BamHI G'GATC_C Cuts at: 3355 3722 3355 Size: 367 5658 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2602 2629 3346 4801 618 Size: 325 217 627 815 27 717 1455 1842 Fragments arranged by size: 1842 1455 815 717 627 325 217 27 BanII G_rGCy'C Cuts at: 1391 2565 3344 1391 Size: 1174 779 4072 Fragments arranged by size: 4072 1174 779 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2332 928 Size: 971 433 4621 Fragments arranged by size: 4621 971 433 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2381 2415 2944 2978 4216 4250 1903 Size: 34 444 34 529 34 1238 34 3678 Fragments arranged by size: 3678 1238 529 444 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3917 5127 3917 Size: 1210 4815 BclI T'GATC_A Cuts at: 1548 1548 Size: 6025 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4636 136 Size: 107 122 71 631 605 2964 1525 Fragments arranged by size: 2964 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6020 6020 Size: 6025 BlpI GC'TnA_GC Cuts at: 3165 3442 3165 Size: 277 5748 Bme1580I G_kGCm'C Cuts at: 1790 3136 4086 5242 1790 Size: 1346 950 1156 2573 Fragments arranged by size: 2573 1346 1156 950 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6025 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3169 3756 4714 Size: 416 53 83 399 24 1752 587 958 Cuts at: 4714 442 Size: 1753 Fragments arranged by size: 1753 1752 958 587 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6025 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2543 3244 4685 1239 Size: 1304 701 1441 2579 Fragments arranged by size: 2579 1441 1304 701 Bpu10I CC'TnA_GC Cuts at: 2709 2709 Size: 6025 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4150 5015 5313 4150 Size: 865 298 4862 Fragments arranged by size: 4862 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4688 882 Size: 617 3189 2219 Fragments arranged by size: 3189 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3816 493 Size: 711 2612 2702 Fragments arranged by size: 2702 2612 711 BsaBI GATnn'nnATC Cuts at: 6019 6019 Size: 6025 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3832 4214 4849 275 Size: 53 83 186 936 2299 382 635 1451 Fragments arranged by size: 2299 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4458 4856 5130 5277 5688 4458 Size: 398 274 147 411 4795 Fragments arranged by size: 4795 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3102 3132 1178 Size: 30 165 30 1699 30 4071 Fragments arranged by size: 4071 1699 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 1186 Size: 913 5112 BseYI C'CCAG_C Cuts at: 2704 5228 5724 2704 Size: 2524 496 3005 Fragments arranged by size: 3005 2524 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2709 1163 Size: 573 973 4479 Fragments arranged by size: 4479 973 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3475 4236 5264 1540 Size: 275 186 1474 761 1028 2301 Fragments arranged by size: 2301 1474 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3136 4086 4171 5242 3136 Size: 950 85 1071 3919 Fragments arranged by size: 3919 1071 950 85 BsmI GAATG_Cn' Cuts at: 3536 3629 3536 Size: 93 5932 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2565 3136 3344 4086 4171 5242 1391 Size: 399 775 571 208 742 85 1071 2174 Fragments arranged by size: 2174 1071 775 742 571 399 208 85 BspEI T'CCGG_A Cuts at: 5688 5688 Size: 6025 BspHI T'CATG_A Cuts at: 3914 5644 3914 Size: 1730 4295 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2695 3233 3380 844 Size: 1851 538 147 3489 Fragments arranged by size: 3489 1851 538 147 BsrBI CCG'CTC Cuts at: 1394 1823 3912 1394 Size: 429 2089 3507 Fragments arranged by size: 3507 2089 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4520 4694 65 Size: 1183 3272 174 1396 Fragments arranged by size: 3272 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2473 4669 4856 1700 Size: 110 663 2196 187 2869 Fragments arranged by size: 2869 2196 663 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6025 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6025 BssSI C'ACGA_G Cuts at: 4085 5097 4085 Size: 1012 5013 BstAPI GCAn_nnn'nTGC Cuts at: 794 2933 794 Size: 2139 3886 BstBI TT'CG_AA Cuts at: 2323 4829 2323 Size: 2506 3519 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6025 BstYI r'GATC_y Cuts at: 1972 2572 2764 3355 3722 4107 4124 5576 5662 Size: 600 192 591 367 385 17 1452 86 Cuts at: 5662 5674 6020 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 600 591 385 367 346 192 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4534 Fragments arranged by size: 4534 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5286 Fragments arranged by size: 5286 683 56 BtsI GCAGTG_nn' Cuts at: 2352 3541 4335 4355 2352 Size: 1189 794 20 4022 Fragments arranged by size: 4022 1189 794 20 ClaI AT'CG_AT Cuts at: 1554 2954 1554 Size: 1400 4625 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5990 DraI TTT'AAA Cuts at: 3329 3329 Size: 6025 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5032 809 Size: 507 3716 1802 Fragments arranged by size: 3716 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3250 3472 4361 Size: 54 1425 50 275 18 1420 222 889 Cuts at: 4361 5990 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1420 889 275 222 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3472 1537 Size: 275 1660 4090 Fragments arranged by size: 4090 1660 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2436 3955 1353 Size: 1083 1519 3423 Fragments arranged by size: 3423 1519 1083 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4612 4986 5132 387 Size: 1064 138 3023 374 146 1280 Fragments arranged by size: 3023 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2543 3244 4088 4685 5472 5740 1239 Size: 393 911 701 844 597 787 268 1524 Fragments arranged by size: 1524 911 844 787 701 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3240 3240 Size: 6025 EcoO109I rG'GnC_Cy Cuts at: 2123 2897 2123 Size: 774 5251 EcoRI G'AATT_C Cuts at: 2178 3334 3712 2178 Size: 1156 378 4491 Fragments arranged by size: 4491 1156 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2324 2324 Size: 6025 FspI TGC'GCA Cuts at: 4531 4531 Size: 6025 HaeII r_GCGC'y Cuts at: 1678 5172 1678 Size: 3494 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2662 2694 4666 4698 4740 Size: 32 135 32 1090 32 1972 32 42 Cuts at: 4740 4772 1373 Size: 32 2626 Fragments arranged by size: 2626 1972 1090 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2224 3369 3615 669 Size: 988 281 286 1145 246 3079 Fragments arranged by size: 3079 1145 988 286 281 246 HindIII A'AGCT_T Cuts at: 748 2072 748 Size: 1324 4701 HpaI GTT'AAC Cuts at: 3615 3615 Size: 6025 KpnI G_GTAC'C Cuts at: 3350 3350 Size: 6025 MfeI C'AATT_G Cuts at: 3624 3624 Size: 6025 MluI A'CGCG_T Cuts at: 5901 5901 Size: 6025 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5139 5323 1305 Size: 3834 184 2007 Fragments arranged by size: 3834 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3252 10 Size: 54 1425 343 1420 2783 Fragments arranged by size: 2783 1425 1420 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2627 3023 3035 3316 3983 4342 4501 Size: 648 1461 396 12 281 667 359 159 Cuts at: 4501 518 Size: 2042 Fragments arranged by size: 2042 1461 667 648 396 359 281 159 12 MspA1I CmG'CkG Cuts at: 1606 2492 2735 2933 3441 4118 5266 5511 1606 Size: 886 243 198 508 677 1148 245 2120 Fragments arranged by size: 2120 1148 886 677 508 245 243 198 NaeI GCC'GGC Cuts at: 2475 2475 Size: 6025 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4534 Fragments arranged by size: 4534 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6025 NgoMIV G'CCGG_C Cuts at: 2473 2473 Size: 6025 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6025 NotI GC'GGCC_GC Cuts at: 3472 3472 Size: 6025 NspI r_CATG'y Cuts at: 1132 1229 2263 3034 3383 4928 1132 Size: 97 1034 771 349 1545 2229 Fragments arranged by size: 2229 1545 1034 771 349 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6025 PciI A'CATG_T Cuts at: 4924 4924 Size: 6025 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6025 PfoI T'CCnGG_A Cuts at: 2138 2138 Size: 6025 PmeI GTTT'AAAC Cuts at: 3329 3329 Size: 6025 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4094 4126 5607 5639 4094 Size: 32 1481 32 4480 Fragments arranged by size: 4480 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2123 2897 2123 Size: 774 5251 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6025 PsiI TTA'TAA Cuts at: 3595 3595 Size: 6025 PstI C_TGCA'G Cuts at: 830 2505 2977 3244 3377 830 Size: 1675 472 267 133 3478 Fragments arranged by size: 3478 1675 472 267 133 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6025 PvuII CAG'CTG Cuts at: 2492 2735 2933 2492 Size: 243 198 5584 Fragments arranged by size: 5584 243 198 SalI G'TCGA_C Cuts at: 1936 3367 1936 Size: 1431 4594 SanDI GG'GwC_CC Cuts at: 2897 2897 Size: 6025 SbfI CC_TGCA'GG Cuts at: 3377 3377 Size: 6025 ScaI AGT'ACT Cuts at: 2099 2878 4273 5985 2099 Size: 779 1395 1712 2139 Fragments arranged by size: 2139 1712 1395 779 SfcI C'TryA_G Cuts at: 826 1045 2501 2748 2973 3240 3373 4508 5189 Size: 219 1456 247 225 267 133 1135 681 Cuts at: 5189 5380 826 Size: 191 1471 Fragments arranged by size: 1471 1456 1135 681 267 247 225 219 191 133 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6025 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6025 SmaI CCC'GGG Cuts at: 1872 3352 1872 Size: 1480 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4129 5030 5292 820 Size: 197 926 2186 901 262 1553 Fragments arranged by size: 2186 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3816 493 Size: 3323 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6025 SphI G_CATG'C Cuts at: 1132 2263 3034 3383 1132 Size: 1131 771 349 3774 Fragments arranged by size: 3774 1131 771 349 SspI AAT'ATT Cuts at: 5 52 2146 3949 5 Size: 47 2094 1803 2081 Fragments arranged by size: 2094 2081 1803 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6025 StyI C'CwwG_G Cuts at: 513 1065 2004 3106 3464 513 Size: 552 939 1102 358 3074 Fragments arranged by size: 3074 1102 939 552 358 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2680 3119 4052 4069 4222 4407 4852 Size: 1255 1100 439 933 17 153 185 445 Cuts at: 4852 325 Size: 1498 Fragments arranged by size: 1498 1255 1100 933 445 439 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2097 2876 4271 Size: 274 80 33 51 1443 120 779 1395 Cuts at: 4271 5983 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1395 779 274 138 120 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2134 2166 3102 3134 5985 6017 2134 Size: 32 936 32 2851 32 2142 Fragments arranged by size: 2851 2142 936 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3009 1316 Size: 1693 4332 XbaI T'CTAG_A Cuts at: 2767 3361 2767 Size: 594 5431 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3049 3146 3049 Size: 97 5928 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6025 XmaI C'CCGG_G Cuts at: 1870 3350 1870 Size: 1480 4545 XmnI GAAnn'nnTTC Cuts at: 4154 4154 Size: 6025 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3833 276 Size: 53 83 186 936 2299 2468 Fragments arranged by size: 2468 2299 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI