(Linear) (Six-base) MAPSORT of: KIEE4094.seq Check: 339 from: 1 to: 1320 KIEE4094.orf With 182 enzymes: SgfI * July 3, 2012 18:33 .. AccI GT'mk_AC Cuts at: 0 742 1320 Size: 742 578 AclI AA'CG_TT Cuts at: 0 1088 1320 Size: 1088 232 AleI CACnn'nnGTG Cuts at: 0 622 1311 1320 Size: 622 689 9 Fragments arranged by size: 689 622 9 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1048 1320 Size: 1048 272 AlwNI CAG_nnn'CTG Cuts at: 0 36 516 730 976 1320 Size: 36 480 214 246 344 Fragments arranged by size: 480 344 246 214 36 ApaLI G'TGCA_C Cuts at: 0 1127 1320 Size: 1127 193 ApoI r'AATT_y Cuts at: 0 173 346 1320 Size: 173 173 974 Fragments arranged by size: 974 173 173 BanI G'GyrC_C Cuts at: 0 597 624 1320 Size: 597 27 696 Fragments arranged by size: 696 597 27 BanII G_rGCy'C Cuts at: 0 560 1320 Size: 560 760 BbsI GAAGACnn'nnnn_ Cuts at: 0 327 1320 Size: 327 993 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 376 410 939 973 1320 Size: 376 34 529 34 347 Fragments arranged by size: 529 376 347 34 34 BlpI GC'TnA_GC Cuts at: 0 1160 1320 Size: 1160 160 Bme1580I G_kGCm'C Cuts at: 0 1131 1320 Size: 1131 189 BmrI ACTGGGnnnn_n' Cuts at: 0 1164 1320 Size: 1164 156 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 538 1239 1320 Size: 538 701 81 Fragments arranged by size: 701 538 81 Bpu10I CC'TnA_GC Cuts at: 0 704 1320 Size: 704 616 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1097 1127 1320 Size: 1097 30 193 Fragments arranged by size: 1097 193 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 94 1320 Size: 94 1226 BseYI C'CCAG_C Cuts at: 0 699 1320 Size: 699 621 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 704 1320 Size: 704 616 BsiHKAI G_wGCw'C Cuts at: 0 1131 1320 Size: 1131 189 Bsp1286I G_dGCh'C Cuts at: 0 560 1131 1320 Size: 560 571 189 Fragments arranged by size: 571 560 189 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 690 1228 1320 Size: 690 538 92 Fragments arranged by size: 690 538 92 BsrFI r'CCGG_y Cuts at: 0 468 1320 Size: 468 852 BstAPI GCAn_nnn'nTGC Cuts at: 0 928 1320 Size: 928 392 BstBI TT'CG_AA Cuts at: 0 318 1320 Size: 318 1002 BstYI r'GATC_y Cuts at: 0 567 759 1320 Size: 567 192 561 Fragments arranged by size: 567 561 192 BtsI GCAGTG_nn' Cuts at: 0 347 1320 Size: 347 973 ClaI AT'CG_AT Cuts at: 0 949 1320 Size: 949 371 EaeI y'GGCC_r Cuts at: 0 1245 1320 Size: 1245 75 EarI CTCTTCn'nnn_ Cuts at: 0 431 1320 Size: 431 889 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 538 1239 1320 Size: 538 701 81 Fragments arranged by size: 701 538 81 EcoNI CCTnn'n_nnAGG Cuts at: 0 1235 1320 Size: 1235 85 EcoO109I rG'GnC_Cy Cuts at: 0 118 892 1320 Size: 118 774 428 Fragments arranged by size: 774 428 118 EcoRI G'AATT_C Cuts at: 0 173 1320 Size: 173 1147 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 319 1320 Size: 319 1001 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 657 689 1320 Size: 657 32 631 Fragments arranged by size: 657 631 32 HincII GTy'rAC Cuts at: 0 219 1320 Size: 219 1101 HindIII A'AGCT_T Cuts at: 0 67 1320 Size: 67 1253 MscI TGG'CCA Cuts at: 0 1247 1320 Size: 1247 73 MslI CAynn'nnrTG Cuts at: 0 622 1018 1030 1311 1320 Size: 622 396 12 281 9 Fragments arranged by size: 622 396 281 12 9 MspA1I CmG'CkG Cuts at: 0 487 730 928 1320 Size: 487 243 198 392 Fragments arranged by size: 487 392 243 198 NaeI GCC'GGC Cuts at: 0 470 1320 Size: 470 850 NgoMIV G'CCGG_C Cuts at: 0 468 1320 Size: 468 852 NspI r_CATG'y Cuts at: 0 258 1029 1320 Size: 258 771 291 Fragments arranged by size: 771 291 258 PfoI T'CCnGG_A Cuts at: 0 133 1320 Size: 133 1187 PpuMI rG'GwC_Cy Cuts at: 0 118 892 1320 Size: 118 774 428 Fragments arranged by size: 774 428 118 PstI C_TGCA'G Cuts at: 0 500 972 1239 1320 Size: 500 472 267 81 Fragments arranged by size: 500 472 267 81 PvuII CAG'CTG Cuts at: 0 487 730 928 1320 Size: 487 243 198 392 Fragments arranged by size: 487 392 243 198 SanDI GG'GwC_CC Cuts at: 0 892 1320 Size: 892 428 ScaI AGT'ACT Cuts at: 0 94 873 1320 Size: 94 779 447 Fragments arranged by size: 779 447 94 SfcI C'TryA_G Cuts at: 0 496 743 968 1235 1320 Size: 496 247 225 267 85 Fragments arranged by size: 496 267 247 225 85 SphI G_CATG'C Cuts at: 0 258 1029 1320 Size: 258 771 291 Fragments arranged by size: 771 291 258 SspI AAT'ATT Cuts at: 0 141 1320 Size: 141 1179 StyI C'CwwG_G Cuts at: 0 1101 1320 Size: 1101 219 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 675 1114 1320 Size: 675 439 206 Fragments arranged by size: 675 439 206 TatI w'GTAC_w Cuts at: 0 92 871 1320 Size: 92 779 449 Fragments arranged by size: 779 449 92 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 129 161 1097 1129 1320 Size: 129 32 936 32 191 Fragments arranged by size: 936 191 129 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1004 1320 Size: 1004 316 XbaI T'CTAG_A Cuts at: 0 762 1320 Size: 762 558 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1044 1141 1320 Size: 1044 97 179 Fragments arranged by size: 1044 179 97 Enzymes that do cut: AccI AclI AleI AlfI AlwNI ApaLI ApoI BanI BanII BbsI BcgI BlpI Bme1580I BmrI BpmI Bpu10I BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrFI BstAPI BstBI BstYI BtsI ClaI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII HindIII MscI MslI MspA1I NaeI NgoMIV NspI PfoI PpuMI PstI PvuII SanDI ScaI SfcI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI Enzymes that do not cut: AarI AatII Acc65I AcuI AfeI AflII AflIII AgeI AhdI AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BciVI BclI BfrBI BglI BglII BmgBI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI MmeI NarI NcoI NdeI NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI StuI SwaI XhoI XmaI XmnI ZraI