(Circular) (Six-base) MAPSORT of: pFN21AB9967.seq Check: 1579 from: 1 to: 6358 pFN21AB9967 6358 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2373 4168 278 Size: 53 83 186 936 837 1795 2468 Fragments arranged by size: 2468 1795 936 837 186 83 53 AccI GT'mk_AC Cuts at: 1937 2373 3701 1937 Size: 436 1328 4594 Fragments arranged by size: 4594 1328 436 Acc65I G'GTAC_C Cuts at: 3256 3679 3256 Size: 423 5935 AclI AA'CG_TT Cuts at: 1563 2517 4485 4858 1563 Size: 954 1968 373 3063 Fragments arranged by size: 3063 1968 954 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2536 2904 2990 4421 5805 6073 1632 Size: 904 368 86 1431 1384 268 1917 Fragments arranged by size: 1917 1431 1384 904 368 268 86 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6161 AflIII A'CryG_T Cuts at: 1203 5257 6234 1203 Size: 4054 977 1327 Fragments arranged by size: 4054 1327 977 AgeI A'CCGG_T Cuts at: 5189 5189 Size: 6358 AhdI GACnn_n'nnGTC Cuts at: 5087 5087 Size: 6358 AleI CACnn'nnGTG Cuts at: 1166 2387 1166 Size: 1221 5137 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6358 AlwNI CAG_nnn'CTG Cuts at: 2187 2769 4101 5673 2187 Size: 582 1332 1572 2872 Fragments arranged by size: 2872 1572 1332 582 ApaI G_GGCC'C Cuts at: 2052 2052 Size: 6358 ApaLI G'TGCA_C Cuts at: 2710 4415 5571 2710 Size: 1705 1156 3497 Fragments arranged by size: 3497 1705 1156 ApoI r'AATT_y Cuts at: 1493 2664 3147 3305 3667 3893 4045 6154 1493 Size: 1171 483 158 362 226 152 2109 1697 Fragments arranged by size: 2109 1697 1171 483 362 226 158 152 AseI AT'TA_AT Cuts at: 160 1032 4912 160 Size: 872 3880 1606 Fragments arranged by size: 3880 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6358 AvaI C'yCGr_G Cuts at: 1870 1943 2288 3683 1870 Size: 73 345 1395 4545 Fragments arranged by size: 4545 1395 345 73 BamHI G'GATC_C Cuts at: 3688 4055 3688 Size: 367 5991 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2233 2428 2797 3256 3679 Size: 325 217 627 446 195 369 459 423 Cuts at: 3679 5134 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 459 446 423 369 325 217 195 BanII G_rGCy'C Cuts at: 1391 2052 3357 3677 1391 Size: 661 1305 320 4072 Fragments arranged by size: 4072 1305 661 320 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3334 928 Size: 971 1435 3952 Fragments arranged by size: 3952 1435 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2135 2169 4549 4583 1903 Size: 34 50 34 114 34 2380 34 3678 Fragments arranged by size: 3678 2380 114 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4250 5460 4250 Size: 1210 5148 BclI T'GATC_A Cuts at: 1548 1548 Size: 6358 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2349 4969 136 Size: 107 122 71 631 605 677 2620 1525 Fragments arranged by size: 2620 1525 677 631 605 122 107 71 BglII A'GATC_T Cuts at: 2625 3590 6353 2625 Size: 965 2763 2630 Fragments arranged by size: 2763 2630 965 BlpI GC'TnA_GC Cuts at: 2078 3351 3775 2078 Size: 1273 424 4661 Fragments arranged by size: 4661 1273 424 Bme1580I G_kGCm'C Cuts at: 1790 2052 2238 2433 2714 4419 5575 1790 Size: 262 186 195 281 1705 1156 2573 Fragments arranged by size: 2573 1705 1156 281 262 195 186 BmgBI CAC'GTC Cuts at: 1318 2311 1318 Size: 993 5365 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4089 5047 442 Size: 416 53 83 399 24 2672 958 1753 Fragments arranged by size: 2672 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6358 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2315 2348 3391 5018 1239 Size: 1076 33 1043 1627 2579 Fragments arranged by size: 2579 1627 1076 1043 33 Bpu10I CC'TnA_GC Cuts at: 2177 2177 Size: 6358 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4483 5348 5646 4483 Size: 865 298 5195 Fragments arranged by size: 5195 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5021 882 Size: 617 3522 2219 Fragments arranged by size: 3522 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4149 493 Size: 711 2945 2702 Fragments arranged by size: 2945 2702 711 BsaBI GATnn'nnATC Cuts at: 3506 6352 3506 Size: 2846 3512 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2166 2370 4165 4547 Size: 53 83 186 936 633 204 1795 382 Cuts at: 4547 5182 275 Size: 635 1451 Fragments arranged by size: 1795 1451 936 635 633 382 204 186 83 53 BsaWI w'CCGG_w Cuts at: 4791 5189 5463 5610 6021 4791 Size: 398 274 147 411 5128 Fragments arranged by size: 5128 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3219 3249 1178 Size: 30 165 30 1816 30 4287 Fragments arranged by size: 4287 1816 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2456 2969 3243 1186 Size: 1270 513 274 4301 Fragments arranged by size: 4301 1270 513 274 BseYI C'CCAG_C Cuts at: 2352 2580 3298 5561 6057 2352 Size: 228 718 2263 496 2653 Fragments arranged by size: 2653 2263 718 496 228 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5785 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2227 3808 4569 5597 1540 Size: 275 186 226 1581 761 1028 2301 Fragments arranged by size: 2301 1581 1028 761 275 226 186 BsiHKAI G_wGCw'C Cuts at: 2148 2466 2714 3357 4419 4504 5575 2148 Size: 318 248 643 1062 85 1071 2931 Fragments arranged by size: 2931 1071 1062 643 318 248 85 BsmI GAATG_Cn' Cuts at: 3468 3653 3869 3962 3468 Size: 185 216 93 5864 Fragments arranged by size: 5864 216 185 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2148 2238 2433 2466 2714 3357 Size: 399 262 96 90 195 33 248 643 Cuts at: 3357 3677 4419 4504 5575 1391 Size: 320 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 643 399 320 262 248 195 96 90 85 33 BspEI T'CCGG_A Cuts at: 6021 6021 Size: 6358 BspHI T'CATG_A Cuts at: 4247 5977 4247 Size: 1730 4628 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3713 844 Size: 2869 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4245 1394 Size: 429 2422 3507 Fragments arranged by size: 3507 2422 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3423 4853 5027 65 Size: 1183 2175 1430 174 1396 Fragments arranged by size: 2175 1430 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2256 5002 5189 1700 Size: 110 446 2746 187 2869 Fragments arranged by size: 2869 2746 446 187 110 BsrGI T'GTAC_A Cuts at: 96 2539 96 Size: 2443 3915 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6358 BssSI C'ACGA_G Cuts at: 4418 5430 4418 Size: 1012 5346 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6358 BstBI TT'CG_AA Cuts at: 5162 5162 Size: 6358 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6358 BstXI CCAn_nnnn'nTGG Cuts at: 2638 3289 2638 Size: 651 5707 BstYI r'GATC_y Cuts at: 1972 2625 3590 3688 4055 4440 4457 5909 5995 Size: 653 965 98 367 385 17 1452 86 Cuts at: 5995 6007 6353 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 965 653 385 367 346 98 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2532 3399 513 Size: 552 1467 867 3472 Fragments arranged by size: 3472 1467 867 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2254 487 Size: 683 56 1028 4591 Fragments arranged by size: 4591 1028 683 56 BtsI GCAGTG_nn' Cuts at: 2940 3345 3874 4668 4688 2940 Size: 405 529 794 20 4610 Fragments arranged by size: 4610 794 529 405 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6358 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6323 DraI TTT'AAA Cuts at: 3662 3662 Size: 6358 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3034 5365 809 Size: 507 1718 2331 1802 Fragments arranged by size: 2331 1802 1718 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2224 3805 4694 Size: 54 1425 50 275 18 394 1581 889 Cuts at: 4694 6323 8 Size: 1629 43 Fragments arranged by size: 1629 1581 1425 889 394 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2224 3805 1537 Size: 275 412 1581 4090 Fragments arranged by size: 4090 1581 412 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3248 3552 4288 1353 Size: 1895 304 736 3423 Fragments arranged by size: 3423 1895 736 304 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2788 4945 5319 5465 387 Size: 1064 138 1199 2157 374 146 1280 Fragments arranged by size: 2157 1280 1199 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3355 3355 Size: 6358 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2315 2348 2536 2904 2990 3391 4421 Size: 393 683 33 188 368 86 401 1030 Cuts at: 4421 5018 5805 6073 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1030 787 683 597 401 393 368 268 188 86 33 EcoO109I rG'GnC_Cy Cuts at: 2048 2049 2136 2048 Size: 1 87 6270 Fragments arranged by size: 6270 87 1 EcoRI G'AATT_C Cuts at: 3667 4045 3667 Size: 378 5980 EcoRV GAT'ATC Cuts at: 2859 2890 2859 Size: 31 6327 FspI TGC'GCA Cuts at: 4864 4864 Size: 6358 HaeII r_GCGC'y Cuts at: 1678 2062 2085 2187 2288 2792 5505 1678 Size: 384 23 102 101 504 2713 2531 Fragments arranged by size: 2713 2531 504 384 102 101 23 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3219 3251 4999 5031 5073 Size: 32 135 32 1647 32 1748 32 42 Cuts at: 5073 5105 1373 Size: 32 2626 Fragments arranged by size: 2626 1748 1647 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3702 3948 669 Size: 988 281 1764 246 3079 Fragments arranged by size: 3079 1764 988 281 246 HindIII A'AGCT_T Cuts at: 748 2982 748 Size: 2234 4124 HpaI GTT'AAC Cuts at: 3948 3948 Size: 6358 KpnI G_GTAC'C Cuts at: 3260 3683 3260 Size: 423 5935 MfeI C'AATT_G Cuts at: 3957 3957 Size: 6358 MluI A'CGCG_T Cuts at: 6234 6234 Size: 6358 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2655 5472 5656 1305 Size: 1350 2817 184 2007 Fragments arranged by size: 2817 2007 1350 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4536 Fragments arranged by size: 4536 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2387 3108 3383 3479 4316 4675 4834 Size: 648 1221 721 275 96 837 359 159 Cuts at: 4834 518 Size: 2042 Fragments arranged by size: 2042 1221 837 721 648 359 275 159 96 MspA1I CmG'CkG Cuts at: 1606 2151 2209 2244 2326 2491 2580 2821 3774 Size: 545 58 35 82 165 89 241 953 Cuts at: 3774 4451 5599 5844 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 953 677 545 245 241 165 89 82 58 35 NcoI C'CATG_G Cuts at: 513 1065 2532 513 Size: 552 1467 4339 Fragments arranged by size: 4339 1467 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6358 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6358 NotI GC'GGCC_GC Cuts at: 3805 3805 Size: 6358 NspI r_CATG'y Cuts at: 1132 1229 3716 5261 1132 Size: 97 2487 1545 2229 Fragments arranged by size: 2487 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6358 PciI A'CATG_T Cuts at: 5257 5257 Size: 6358 PflMI CCAn_nnn'nTGG Cuts at: 1108 2580 1108 Size: 1472 4886 PmeI GTTT'AAAC Cuts at: 3662 3662 Size: 6358 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4427 4459 5940 5972 4427 Size: 32 1481 32 4813 Fragments arranged by size: 4813 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2136 2136 Size: 6358 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6358 PsiI TTA'TAA Cuts at: 3928 3928 Size: 6358 PspOMI G'GGCC_C Cuts at: 2048 2048 Size: 6358 PstI C_TGCA'G Cuts at: 830 2249 2565 3214 3710 830 Size: 1419 316 649 496 3478 Fragments arranged by size: 3478 1419 649 496 316 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6358 PvuII CAG'CTG Cuts at: 2244 2326 2491 2580 2244 Size: 82 165 89 6022 Fragments arranged by size: 6022 165 89 82 SacI G_AGCT'C Cuts at: 3357 3357 Size: 6358 SalI G'TCGA_C Cuts at: 1936 3700 1936 Size: 1764 4594 SanDI GG'GwC_CC Cuts at: 2136 2136 Size: 6358 SbfI CC_TGCA'GG Cuts at: 3710 3710 Size: 6358 ScaI AGT'ACT Cuts at: 3216 4606 6318 3216 Size: 1390 1712 3256 Fragments arranged by size: 3256 1712 1390 SfcI C'TryA_G Cuts at: 826 1045 2245 2561 3210 3706 4841 5522 5713 Size: 219 1200 316 649 496 1135 681 191 Cuts at: 5713 826 Size: 1471 Fragments arranged by size: 1471 1200 1135 681 649 496 316 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6358 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6358 SmaI CCC'GGG Cuts at: 1872 3685 1872 Size: 1813 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4462 5363 5625 820 Size: 197 926 2519 901 262 1553 Fragments arranged by size: 2519 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4149 493 Size: 3656 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6358 SphI G_CATG'C Cuts at: 1132 3716 1132 Size: 2584 3774 SspI AAT'ATT Cuts at: 5 52 3016 4282 5 Size: 47 2964 1266 2081 Fragments arranged by size: 2964 2081 1266 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6358 StyI C'CwwG_G Cuts at: 513 1065 2532 3636 3797 513 Size: 552 1467 1104 161 3074 Fragments arranged by size: 3074 1467 1104 552 161 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2408 4385 4402 4555 4740 5185 325 Size: 1255 828 1977 17 153 185 445 1498 Fragments arranged by size: 1977 1498 1255 828 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2539 3214 4604 Size: 274 80 33 51 1443 562 675 1390 Cuts at: 4604 6316 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1390 675 562 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6318 6350 6318 Size: 32 6326 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6358 XbaI T'CTAG_A Cuts at: 3694 3694 Size: 6358 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6358 XmaI C'CCGG_G Cuts at: 1870 3683 1870 Size: 1813 4545 XmnI GAAnn'nnTTC Cuts at: 2984 3125 3584 4487 2984 Size: 141 459 903 4855 Fragments arranged by size: 4855 903 459 141 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2371 4166 276 Size: 53 83 186 936 837 1795 2468 Fragments arranged by size: 2468 1795 936 837 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI