################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AviII AvrII BalI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BspMI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FbaI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI TfiI Tru9I Tsp509I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccII 305 CGCG CG^CG AfaI 820 GTAC GT^AC AspI 75 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 832 GGATCC G^GATCC BlpI 36 GCTGAGC GC^T[ACGT]AGC Bpu1102I 36 GCTGAGC GC^T[ACGT]AGC BsaI 728 GAGACC GGTCTC(1/5) BseRI 99 CTCCTC GAGGAG(10/8) BsmAI 728 GAGAC GTCTC(1/5) Bsp1407I 819 TGTACA T^GTACA BsrBI 168 CCGCTC CCGCTC(-3/-3) BsrFI 610 GCCGGC [GA]^CCGG[CT] BsrGI 819 TGTACA T^GTACA BstEII 147 GGTCACC G^GT[ACGT]ACC BstPI 147 GGTCACC G^GT[ACGT]ACC BstUI 305 CGCG CG^CG BstXI 176 CCAGCCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 36 GCTGAGC GC^T[ACGT]AGC Cfr10I 610 GCCGGC [GA]^CCGG[CT] DrdI 883 GACTCTCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 725 GAAGAG CTCTTC(1/4) EcoO65I 147 GGTCACC G^GT[ACGT]ACC FseI 609 GGCCGGCC GGCCGG^CC HaeII 347 GGCGCT [GA]GCGC^[CT] HgaI 779 GCGTC GACGC(5/10) Ksp632I 725 GAAGAG CTCTTC(1/4) MaeII 47 ACGT A^CGT MvnI 305 CGCG CG^CG NaeI 610 GCCGGC GCC^GGC NgoMIV 610 GCCGGC G^CCGGC PflFI 75 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuII 195 CAGCTG CAG^CTG RsaI 820 GTAC GT^AC SfiI 604 GGCCTGGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 522 CCCGGG CCC^GGG SspBI 819 TGTACA T^GTACA StuI 603 AGGCCT AGG^CCT Tth111I 75 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 522 CCCGGG C^CCGGG XmaI 522 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AlwNI 777 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG 823 CAGTCCCTG ApaI 249 GGGCCC GGGCC^C 787 GGGCCC AvaI 102 CTCGGG C^[CT]CG[GA]G 522 CCCGGG BanI 207 GGTGCC G^G[CT][GA]CC 709 GGCACC BbsI 508 GAAGAC GAAGAC(2/6) 892 GTCTTC BfaI 181 CTAG C^TAG 503 CTAG BpuAI 508 GAAGAC GAAGAC(2/6) 892 GTCTTC BsoBI 102 CTCGGG C^[CT]CG[GA]G 522 CCCGGG BsrI 372 CCAGT ACTGG(1/-1) 739 CCAGT BstF5I 59 CATCC GGATG(2/0) 492 CATCC BstYI 3 GGATCT [GA]^GATC[CT] 832 GGATCC DpnII 4 GATC ^GATC 833 GATC EcoNI 180 CCTAGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 755 CCTGCCGAAGG FokI 59 CATCC GGATG(9/13) 492 CATCC MaeI 181 CTAG C^TAG 503 CTAG MboI 4 GATC ^GATC 833 GATC MflI 3 GGATCT [GA]^GATC[CT] 832 GGATCC NcoI 66 CCATGG C^CATGG 381 CCATGG NdeII 4 GATC ^GATC 833 GATC NlaIII 67 CATG CATG^ 382 CATG PpuMI 105 GGGACCC [GA]G^G[AT]CC[CT] 668 GGGACCT Sau3AI 4 GATC ^GATC 833 GATC SfaNI 551 GCATC GCATC(5/9) 962 GCATC TaqI 723 TCGA T^CGA 896 TCGA TspRI 282 CACTG CA[GC]TG[ACGT][ACGT]^ 677 CACTG TthHB8I 723 TCGA T^CGA 896 TCGA XcmI 173 CCACCAGCCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 372 CCAGTTTGCCCATGG XhoII 3 GGATCT [GA]^GATC[CT] 832 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AluI AlwI AvaII BanII BbvI BcnI BglI BmyI BpmI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I DdeI DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboII MnlI MspA1I MspI MvaI MwoI NciI NlaIV PflMI PleI Sau96I ScrFI StyI TseI Tsp45I Van91I