(Circular) (Six-base) MAPSORT of: pFN21AE4323.seq Check: 3212 from: 1 to: 5683 pFN21AE4323 5683 bp With 182 enzymes: SgfI * July 6, 2012 17:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3493 278 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3026 1937 Size: 1089 4594 Acc65I G'GTAC_C Cuts at: 3004 3004 Size: 5683 AclI AA'CG_TT Cuts at: 1563 3810 4183 1563 Size: 2247 373 3063 Fragments arranged by size: 3063 2247 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3746 5130 5398 1632 Size: 2114 1384 268 1917 Fragments arranged by size: 2114 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5486 AflIII A'CryG_T Cuts at: 1203 4582 5559 1203 Size: 3379 977 1327 Fragments arranged by size: 3379 1327 977 AgeI A'CCGG_T Cuts at: 4514 4514 Size: 5683 AhdI GACnn_n'nnGTC Cuts at: 4412 4412 Size: 5683 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5683 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5683 AlwNI CAG_nnn'CTG Cuts at: 2787 2833 3426 4998 2787 Size: 46 593 1572 3472 Fragments arranged by size: 3472 1572 593 46 ApaI G_GGCC'C Cuts at: 2258 2796 2258 Size: 538 5145 ApaLI G'TGCA_C Cuts at: 3740 4896 3740 Size: 1156 4527 ApoI r'AATT_y Cuts at: 1493 2992 3218 3370 5479 1493 Size: 1499 226 152 2109 1697 Fragments arranged by size: 2109 1697 1499 226 152 AseI AT'TA_AT Cuts at: 160 1032 4237 160 Size: 872 3205 1606 Fragments arranged by size: 3205 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 AvaI C'yCGr_G Cuts at: 1870 1943 2107 2527 3008 1870 Size: 73 164 420 481 4545 Fragments arranged by size: 4545 481 420 164 73 BamHI G'GATC_C Cuts at: 2837 3013 3380 2837 Size: 176 367 5140 Fragments arranged by size: 5140 367 176 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2212 2714 3004 4459 618 Size: 325 217 627 425 502 290 1455 1842 Fragments arranged by size: 1842 1455 627 502 425 325 290 217 BanII G_rGCy'C Cuts at: 1391 2048 2105 2129 2258 2796 2885 3002 1391 Size: 657 57 24 129 538 89 117 4072 Fragments arranged by size: 4072 657 538 129 117 89 57 24 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2520 2890 928 Size: 971 621 370 3721 Fragments arranged by size: 3721 971 621 370 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3874 3908 1903 Size: 34 1937 34 3678 Fragments arranged by size: 3678 1937 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3575 4785 3575 Size: 1210 4473 BclI T'GATC_A Cuts at: 1548 1548 Size: 5683 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2169 2478 2616 Size: 107 122 71 631 605 497 309 138 Cuts at: 2616 2880 4294 136 Size: 264 1414 1525 Fragments arranged by size: 1525 1414 631 605 497 309 264 138 122 107 71 BglII A'GATC_T Cuts at: 5678 5678 Size: 5683 BlpI GC'TnA_GC Cuts at: 2042 3100 2042 Size: 1058 4625 Bme1580I G_kGCm'C Cuts at: 1790 2058 2217 2258 2796 3744 4900 1790 Size: 268 159 41 538 948 1156 2573 Fragments arranged by size: 2573 1156 948 538 268 159 41 BmgBI CAC'GTC Cuts at: 1318 2053 1318 Size: 735 4948 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2738 3414 4372 Size: 416 53 83 399 24 1321 676 958 Cuts at: 4372 442 Size: 1753 Fragments arranged by size: 1753 1321 958 676 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5683 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2122 2137 2444 2617 2635 4343 1239 Size: 883 15 307 173 18 1708 2579 Fragments arranged by size: 2579 1708 883 307 173 18 15 Bpu10I CC'TnA_GC Cuts at: 2123 2259 2123 Size: 136 5547 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3808 4673 4971 3808 Size: 865 298 4520 Fragments arranged by size: 4520 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2727 4346 882 Size: 617 1228 1619 2219 Fragments arranged by size: 2219 1619 1228 617 BsaAI yAC'GTr Cuts at: 493 1204 3474 493 Size: 711 2270 2702 Fragments arranged by size: 2702 2270 711 BsaBI GATnn'nnATC Cuts at: 5677 5677 Size: 5683 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3490 3872 4507 275 Size: 53 83 186 936 1957 382 635 1451 Fragments arranged by size: 1957 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4116 4514 4788 4935 5346 4116 Size: 398 274 147 411 4453 Fragments arranged by size: 4453 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5458 Fragments arranged by size: 5458 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2095 1186 Size: 909 4774 BseYI C'CCAG_C Cuts at: 2097 2208 2925 4886 5382 2097 Size: 111 717 1961 496 2398 Fragments arranged by size: 2398 1961 717 496 111 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5110 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3133 3894 4922 1540 Size: 275 186 1132 761 1028 2301 Fragments arranged by size: 2301 1132 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3744 3829 4900 3744 Size: 85 1071 4527 Fragments arranged by size: 4527 1071 85 BsmI GAATG_Cn' Cuts at: 3194 3287 3194 Size: 93 5590 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2058 2105 2129 2217 2258 2796 Size: 399 258 10 47 24 88 41 538 Cuts at: 2796 2885 3002 3744 3829 4900 1391 Size: 89 117 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 538 399 258 117 89 88 85 47 41 24 10 BspEI T'CCGG_A Cuts at: 5346 5346 Size: 5683 BspHI T'CATG_A Cuts at: 3572 5302 3572 Size: 1730 3953 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3038 844 Size: 2194 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2175 3570 1394 Size: 429 352 1395 3507 Fragments arranged by size: 3507 1395 429 352 BsrDI GCAATG_nn' Cuts at: 65 1248 4178 4352 65 Size: 1183 2930 174 1396 Fragments arranged by size: 2930 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2615 4327 4514 1700 Size: 110 805 1712 187 2869 Fragments arranged by size: 2869 1712 805 187 110 BsrGI T'GTAC_A Cuts at: 96 2824 96 Size: 2728 2955 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5683 BssSI C'ACGA_G Cuts at: 3743 4755 3743 Size: 1012 4671 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5683 BstBI TT'CG_AA Cuts at: 4487 4487 Size: 5683 BstEII G'GTnAC_C Cuts at: 1184 2152 1184 Size: 968 4715 BstXI CCAn_nnnn'nTGG Cuts at: 2188 2188 Size: 5683 BstYI r'GATC_y Cuts at: 1972 2008 2837 3013 3380 3765 3782 5234 5320 Size: 36 829 176 367 385 17 1452 86 Cuts at: 5320 5332 5678 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 829 385 367 346 176 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2071 2386 2931 513 Size: 552 939 67 315 545 3265 Fragments arranged by size: 3265 939 552 545 315 67 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4944 Fragments arranged by size: 4944 683 56 BtsI GCAGTG_nn' Cuts at: 2286 3199 3993 4013 2286 Size: 913 794 20 3956 Fragments arranged by size: 3956 913 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5683 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5648 DraI TTT'AAA Cuts at: 2987 2987 Size: 5683 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2894 4690 809 Size: 507 1578 1796 1802 Fragments arranged by size: 1802 1796 1578 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2170 2581 2613 Size: 54 1425 50 275 18 340 411 32 Cuts at: 2613 3130 4019 5648 8 Size: 517 889 1629 43 Fragments arranged by size: 1629 1425 889 517 411 340 275 54 50 43 32 18 EagI C'GGCC_G Cuts at: 1537 1812 3130 1537 Size: 275 1318 4090 Fragments arranged by size: 4090 1318 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2725 3613 1353 Size: 1372 888 3423 Fragments arranged by size: 3423 1372 888 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4270 4644 4790 387 Size: 1064 138 2681 374 146 1280 Fragments arranged by size: 2681 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2122 2137 2444 2617 2635 3746 4343 Size: 393 490 15 307 173 18 1111 597 Cuts at: 4343 5130 5398 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1111 787 597 490 393 307 268 173 18 15 EcoNI CCTnn'n_nnAGG Cuts at: 2189 2764 2189 Size: 575 5108 EcoO109I rG'GnC_Cy Cuts at: 2111 2255 2674 2792 2793 2111 Size: 144 419 118 1 5001 Fragments arranged by size: 5001 419 144 118 1 EcoRI G'AATT_C Cuts at: 2992 3370 2992 Size: 378 5305 FseI GG_CCGG'CC Cuts at: 2619 2619 Size: 5683 FspI TGC'GCA Cuts at: 4189 4189 Size: 5683 HaeII r_GCGC'y Cuts at: 1678 2356 4830 1678 Size: 678 2474 2531 Fragments arranged by size: 2531 2474 678 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2940 2972 4324 4356 4398 Size: 32 135 32 1368 32 1352 32 42 Cuts at: 4398 4430 1373 Size: 32 2626 Fragments arranged by size: 2626 1368 1352 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3027 3273 669 Size: 988 281 1089 246 3079 Fragments arranged by size: 3079 1089 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5683 HpaI GTT'AAC Cuts at: 3273 3273 Size: 5683 KpnI G_GTAC'C Cuts at: 3008 3008 Size: 5683 MfeI C'AATT_G Cuts at: 3282 3282 Size: 5683 MluI A'CGCG_T Cuts at: 5559 5559 Size: 5683 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2091 2157 2312 2865 4797 4981 1305 Size: 786 66 155 553 1932 184 2007 Fragments arranged by size: 2007 1932 786 553 184 155 66 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3861 Fragments arranged by size: 3861 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3641 4000 4159 518 Size: 648 2475 359 159 2042 Fragments arranged by size: 2475 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2202 2239 2469 2865 3099 3776 4924 5169 Size: 596 37 230 396 234 677 1148 245 Cuts at: 5169 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 596 396 245 234 230 37 NaeI GCC'GGC Cuts at: 2617 2617 Size: 5683 NcoI C'CATG_G Cuts at: 513 1065 2004 2071 2386 513 Size: 552 939 67 315 3810 Fragments arranged by size: 3810 939 552 315 67 NdeI CA'TA_TG Cuts at: 387 387 Size: 5683 NgoMIV G'CCGG_C Cuts at: 2615 2615 Size: 5683 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5683 NotI GC'GGCC_GC Cuts at: 3130 3130 Size: 5683 NspI r_CATG'y Cuts at: 1132 1229 3041 4586 1132 Size: 97 1812 1545 2229 Fragments arranged by size: 2229 1812 1545 97 PasI CC'CwG_GG Cuts at: 1348 2833 1348 Size: 1485 4198 PciI A'CATG_T Cuts at: 4582 4582 Size: 5683 PflMI CCAn_nnn'nTGG Cuts at: 1108 2225 2392 2669 2932 1108 Size: 1117 167 277 263 3859 Fragments arranged by size: 3859 1117 277 263 167 PmeI GTTT'AAAC Cuts at: 2987 2987 Size: 5683 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3752 3784 5265 5297 3752 Size: 32 1481 32 4138 Fragments arranged by size: 4138 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2111 2674 2111 Size: 563 5120 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5683 PsiI TTA'TAA Cuts at: 3253 3253 Size: 5683 PspOMI G'GGCC_C Cuts at: 2254 2792 2254 Size: 538 5145 PstI C_TGCA'G Cuts at: 830 3035 830 Size: 2205 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5683 PvuII CAG'CTG Cuts at: 2202 2202 Size: 5683 SalI G'TCGA_C Cuts at: 1936 3025 1936 Size: 1089 4594 SanDI GG'GwC_CC Cuts at: 2111 2111 Size: 5683 SbfI CC_TGCA'GG Cuts at: 3035 3035 Size: 5683 ScaI AGT'ACT Cuts at: 3931 5643 3931 Size: 1712 3971 SfcI C'TryA_G Cuts at: 826 1045 3031 4166 4847 5038 826 Size: 219 1986 1135 681 191 1471 Fragments arranged by size: 1986 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2616 2616 Size: 5683 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5683 SmaI CCC'GGG Cuts at: 1872 2529 3010 1872 Size: 657 481 4545 Fragments arranged by size: 4545 657 481 SmlI C'TyrA_G Cuts at: 820 1017 1943 3787 4688 4950 820 Size: 197 926 1844 901 262 1553 Fragments arranged by size: 1844 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3474 493 Size: 2981 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5683 SphI G_CATG'C Cuts at: 1132 3041 1132 Size: 1909 3774 SspI AAT'ATT Cuts at: 5 52 3607 5 Size: 47 3555 2081 Fragments arranged by size: 3555 2081 47 StuI AGG'CCT Cuts at: 1517 2610 1517 Size: 1093 4590 StyI C'CwwG_G Cuts at: 513 1065 2004 2071 2386 2796 3122 513 Size: 552 939 67 315 410 326 3074 Fragments arranged by size: 3074 939 552 410 326 315 67 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2200 2290 2789 3710 3727 3880 4065 Size: 1255 620 90 499 921 17 153 185 Cuts at: 4065 4510 325 Size: 445 1498 Fragments arranged by size: 1498 1255 921 620 499 445 185 153 90 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2824 3929 5641 Size: 274 80 33 51 1443 847 1105 1712 Cuts at: 5641 96 Size: 138 Fragments arranged by size: 1712 1443 1105 847 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5643 5675 5643 Size: 32 5651 Tth111I GACn'n_nGTC Cuts at: 1316 2083 1316 Size: 767 4916 XbaI T'CTAG_A Cuts at: 3019 3019 Size: 5683 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2185 2384 2185 Size: 199 5484 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5683 XmaI C'CCGG_G Cuts at: 1870 2527 3008 1870 Size: 657 481 4545 Fragments arranged by size: 4545 657 481 XmnI GAAnn'nnTTC Cuts at: 3812 3812 Size: 5683 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3491 276 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfoI SrfI SwaI