################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII Acc65I AccI AccIII AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BalI BbrPI BcgI BciVI BclI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiHKAI BsiWI BsmBI BsmI BspDI BspEI BspHI BsrBI BsrDI BssHII Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FbaI FokI FseI FspI HgaI HincII HindII HindIII HpaI HphI KpnI KspI MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NdeI NheI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfaNI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I Tth111I XbaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AclI 178 AACGTT AA^CGTT AcsI 528 GAATTT [GA]^AATT[CT] ApaI 1178 GGGCCC GGGCC^C ApoI 528 GAATTT [GA]^AATT[CT] Asp700I 465 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 687 GGATCC G^GATCC BanII 1178 GGGCCC G[GA]GC[CT]^C BbsI 538 GAAGAC GAAGAC(2/6) BbvCI 388 GCTGAGG CCTCAGC(-5/-2) BmyI 1178 GGGCCC G[AGT]GC[ACT]^C BpuAI 538 GAAGAC GAAGAC(2/6) BsgI 547 GTGCAG GTGCAG(16/14) BsiEI 347 CGACCG CG[GA][CT]^CG Bsp1286I 1178 GGGCCC G[AGT]GC[ACT]^C Bsp1407I 1164 TGTACA T^GTACA BspMI 549 GCAGGT ACCTGC(4/8) BsrFI 366 GCCGGC [GA]^CCGG[CT] BsrGI 1164 TGTACA T^GTACA BsrI 1150 ACTGG ACTGG(1/-1) BssSI 238 CACGAG CACGAG(-5/-1) BstYI 687 GGATCC [GA]^GATC[CT] Cfr10I 366 GCCGGC [GA]^CCGG[CT] DpnII 688 GATC ^GATC EcoT22I 1202 ATGCAT ATGCA^T FauI 14 GCGGG CCCGC(4/6) MboI 688 GATC ^GATC MflI 687 GGATCC [GA]^GATC[CT] NaeI 366 GCCGGC GCC^GGC NdeII 688 GATC ^GATC NgoMIV 366 GCCGGC G^CCGGC NsiI 1202 ATGCAT ATGCA^T PaeR7I 310 CTCGAG C^TCGAG PflMI 636 CCACCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 4 GAGTC GAGTC(4/5) Psp1406I 178 AACGTT AA^CGTT PstI 451 CTGCAG CTGCA^G PvuII 448 CAGCTG CAG^CTG SapI 569 GAAGAGC GCTCTTC(1/4) Sau3AI 688 GATC ^GATC SfcI 451 CTGCAG C^T[GA][CT]AG SmlI 310 CTCGAG C^T[CT][GA]AG SspBI 1164 TGTACA T^GTACA TfiI 478 GAATC G^A[AT]TC Tsp509I 529 AATT ^AATT Van91I 636 CCACCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 310 CTCGAG C^TCGAG XhoII 687 GGATCC [GA]^GATC[CT] XmnI 465 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AflIII 647 ACATGT A^C[GA][CT]GT 1137 ACATGT AlwI 688 GATCC GGATC(4/5) 687 GGATC BfaI 460 CTAG C^TAG 725 CTAG BsmAI 510 GAGAC GTCTC(1/5) 6 GTCTC BspLU11I 647 ACATGT A^CATGT 1137 ACATGT Cac8I 366 GCCGGC GC[ACGT]^[ACGT]GC 520 GCACGC DdeI 152 CTGAG C^T[ACGT]AG 389 CTGAG DraII 661 GGGGCCT [GA]G^G[ACGT]CC[CT] 1178 GGGCCCC EarI 8 CTCTTC CTCTTC(1/4) 569 GAAGAG EcoO109I 661 GGGGCCT [GA]G^G[ACGT]CC[CT] 1178 GGGCCCC HinfI 4 GAGTC G^A[ACGT]TC 478 GAATC Ksp632I 8 CTCTTC CTCTTC(1/4) 569 GAAGAG MaeI 460 CTAG C^TAG 725 CTAG MaeII 179 ACGT A^CGT 494 ACGT MaeIII 97 GTGAC ^GT[ACGT]AC 1134 GTCAC MspA1I 386 CCGCTG C[AC]G^C[GT]G 448 CAGCTG Tsp45I 97 GTGAC ^GT[GC]AC 1134 GTCAC XcmI 24 CCACAGTGATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 761 CCAGGCCACCCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AvaI AvaII BanI BbeI BbvI BcnI BglI BsaHI BsaJI BseRI BsiYI BslI BsmFI BsoBI BssKI BstNI BstUI CfoI Cfr13I DsaI EaeI EcoRII EcoT14I Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI KasI MboII MnlI MspI MvaI MvnI MwoI NarI NciI NcoI NlaIII NlaIV NspI RsaI Sau96I ScrFI SfoI SmaI StyI TaqI TseI TspRI TthHB8I XmaCI XmaI