(Circular) (Six-base) MAPSORT of: pFN21AB7678.seq Check: 139 from: 1 to: 5923 pFN21AB7678 5923 bp With 182 enzymes: SgfI * July 6, 2012 17:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3733 278 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3266 1937 Size: 1329 4594 Acc65I G'GTAC_C Cuts at: 3244 3244 Size: 5923 AclI AA'CG_TT Cuts at: 1563 2184 4050 4423 1563 Size: 621 1866 373 3063 Fragments arranged by size: 3063 1866 621 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2342 2562 3986 5370 5638 1632 Size: 710 220 1424 1384 268 1917 Fragments arranged by size: 1917 1424 1384 710 268 220 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5726 AflIII A'CryG_T Cuts at: 1203 2652 3142 4822 5799 1203 Size: 1449 490 1680 977 1327 Fragments arranged by size: 1680 1449 1327 977 490 AgeI A'CCGG_T Cuts at: 4754 4754 Size: 5923 AhdI GACnn_n'nnGTC Cuts at: 4652 4652 Size: 5923 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5923 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5923 AlwNI CAG_nnn'CTG Cuts at: 3666 5238 3666 Size: 1572 4351 ApaI G_GGCC'C Cuts at: 3187 3187 Size: 5923 ApaLI G'TGCA_C Cuts at: 3980 5136 3980 Size: 1156 4767 ApoI r'AATT_y Cuts at: 1493 2533 3232 3458 3610 5719 1493 Size: 1040 699 226 152 2109 1697 Fragments arranged by size: 2109 1697 1040 699 226 152 AseI AT'TA_AT Cuts at: 160 1032 4477 160 Size: 872 3445 1606 Fragments arranged by size: 3445 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 AvaI C'yCGr_G Cuts at: 1870 1943 2315 2864 2972 2999 3026 3053 3080 Size: 73 372 549 108 27 27 27 27 Cuts at: 3080 3107 3248 1870 Size: 27 141 4545 Fragments arranged by size: 4545 549 372 141 108 73 27 27 27 27 27 BamHI G'GATC_C Cuts at: 2692 3253 3620 2692 Size: 561 367 4995 Fragments arranged by size: 4995 561 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2716 2824 2851 2878 2905 Size: 325 217 627 929 108 27 27 27 Cuts at: 2905 2932 2959 3040 3067 3094 3121 3244 4699 Size: 27 27 81 27 27 27 123 1455 Cuts at: 4699 618 Size: 1842 Fragments arranged by size: 1842 1455 929 627 325 217 123 108 81 27 27 27 27 27 27 27 27 BanII G_rGCy'C Cuts at: 1391 3187 3242 1391 Size: 1796 55 4072 Fragments arranged by size: 4072 1796 55 BbeI G_GCGC'C Cuts at: 2828 2855 2882 2909 2936 2963 3044 3071 3098 Size: 27 27 27 27 27 81 27 27 Cuts at: 3098 3125 2828 Size: 27 5626 Fragments arranged by size: 5626 81 27 27 27 27 27 27 27 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2550 928 Size: 971 651 4301 Fragments arranged by size: 4301 971 651 BbvCI CC'TCA_GC Cuts at: 2394 2394 Size: 5923 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4114 4148 1903 Size: 34 2177 34 3678 Fragments arranged by size: 3678 2177 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3815 5025 3815 Size: 1210 4713 BclI T'GATC_A Cuts at: 1548 1548 Size: 5923 BfrBI ATG'CAT Cuts at: 3209 3209 Size: 5923 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2767 2794 2866 Size: 107 122 71 631 605 1095 27 72 Cuts at: 2866 2893 2920 2947 2974 3001 3028 3055 3082 Size: 27 27 27 27 27 27 27 27 Cuts at: 3082 3109 4534 136 Size: 27 1425 1525 Fragments arranged by size: 1525 1425 1095 631 605 122 107 72 71 27 27 27 27 27 27 27 27 27 27 BglII A'GATC_T Cuts at: 5918 5918 Size: 5923 BlpI GC'TnA_GC Cuts at: 3340 3340 Size: 5923 Bme1580I G_kGCm'C Cuts at: 1790 3187 3984 5140 1790 Size: 1397 797 1156 2573 Fragments arranged by size: 2573 1397 1156 797 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5923 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3654 4612 442 Size: 416 53 83 399 24 2237 958 1753 Fragments arranged by size: 2237 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5923 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4583 1239 Size: 3344 2579 Bpu10I CC'TnA_GC Cuts at: 2394 2394 Size: 5923 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4048 4913 5211 4048 Size: 865 298 4760 Fragments arranged by size: 4760 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4586 882 Size: 617 3087 2219 Fragments arranged by size: 3087 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3714 493 Size: 711 2510 2702 Fragments arranged by size: 2702 2510 711 BsaBI GATnn'nnATC Cuts at: 5917 5917 Size: 5923 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2825 2852 2879 2906 Size: 53 83 186 936 1292 27 27 27 Cuts at: 2906 2933 2960 3041 3068 3095 3122 3730 4112 Size: 27 27 81 27 27 27 608 382 Cuts at: 4112 4747 275 Size: 635 1451 Fragments arranged by size: 1451 1292 936 635 608 382 186 83 81 53 27 27 27 27 27 27 27 27 BsaWI w'CCGG_w Cuts at: 4356 4754 5028 5175 5586 4356 Size: 398 274 147 411 4693 Fragments arranged by size: 4693 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5698 Fragments arranged by size: 5698 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2139 2150 2279 2315 2351 1186 Size: 953 11 129 36 36 4758 Fragments arranged by size: 4758 953 129 36 36 11 BseYI C'CCAG_C Cuts at: 5126 5622 5126 Size: 496 5427 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2573 1163 Size: 573 837 4513 Fragments arranged by size: 4513 837 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2355 3373 4134 5162 1540 Size: 275 186 354 1018 761 1028 2301 Fragments arranged by size: 2301 1028 1018 761 354 275 186 BsiHKAI G_wGCw'C Cuts at: 3984 4069 5140 3984 Size: 85 1071 4767 Fragments arranged by size: 4767 1071 85 BsmI GAATG_Cn' Cuts at: 3434 3527 3434 Size: 93 5830 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3187 3242 3984 4069 5140 1391 Size: 399 1397 55 742 85 1071 2174 Fragments arranged by size: 2174 1397 1071 742 399 85 55 BspEI T'CCGG_A Cuts at: 5586 5586 Size: 5923 BspHI T'CATG_A Cuts at: 3812 5542 3812 Size: 1730 4193 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2545 3278 844 Size: 1701 733 3489 Fragments arranged by size: 3489 1701 733 BsrBI CCG'CTC Cuts at: 1394 1823 3810 1394 Size: 429 1987 3507 Fragments arranged by size: 3507 1987 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4418 4592 65 Size: 1183 3170 174 1396 Fragments arranged by size: 3170 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2371 4567 4754 1700 Size: 110 561 2196 187 2869 Fragments arranged by size: 2869 2196 561 187 110 BsrGI T'GTAC_A Cuts at: 96 3169 96 Size: 3073 2850 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5923 BssSI C'ACGA_G Cuts at: 2243 3983 4995 2243 Size: 1740 1012 3171 Fragments arranged by size: 3171 1740 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5923 BstBI TT'CG_AA Cuts at: 4727 4727 Size: 5923 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5923 BstYI r'GATC_y Cuts at: 1972 2692 3253 3620 4005 4022 5474 5560 5572 Size: 720 561 367 385 17 1452 86 12 Cuts at: 5572 5918 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 720 561 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2355 2775 2820 2901 2928 2955 Size: 552 939 351 420 45 81 27 27 Cuts at: 2955 2982 3009 3036 3063 3090 3117 513 Size: 27 27 27 27 27 27 3319 Fragments arranged by size: 3319 939 552 420 351 81 45 27 27 27 27 27 27 27 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5184 Fragments arranged by size: 5184 683 56 BtsI GCAGTG_nn' Cuts at: 2619 3439 4233 4253 2619 Size: 820 794 20 4289 Fragments arranged by size: 4289 820 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5923 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5888 DraI TTT'AAA Cuts at: 3227 3227 Size: 5923 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4930 809 Size: 507 3614 1802 Fragments arranged by size: 3614 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2239 2894 2921 Size: 54 1425 50 275 18 409 655 27 Cuts at: 2921 3370 4259 5888 8 Size: 449 889 1629 43 Fragments arranged by size: 1629 1425 889 655 449 409 275 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 3370 1537 Size: 275 1558 4090 Fragments arranged by size: 4090 1558 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2019 2569 3853 1353 Size: 666 550 1284 3423 Fragments arranged by size: 3423 1284 666 550 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2390 4510 4884 5030 387 Size: 1064 138 801 2120 374 146 1280 Fragments arranged by size: 2120 1280 1064 801 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2342 2562 3986 4583 5370 5638 1239 Size: 393 710 220 1424 597 787 268 1524 Fragments arranged by size: 1524 1424 787 710 597 393 268 220 EcoO109I rG'GnC_Cy Cuts at: 2667 3184 2667 Size: 517 5406 EcoRI G'AATT_C Cuts at: 3232 3610 3232 Size: 378 5545 FspI TGC'GCA Cuts at: 4429 4429 Size: 5923 HaeII r_GCGC'y Cuts at: 1678 2828 2855 2882 2909 2936 2963 3044 3071 Size: 1150 27 27 27 27 27 81 27 Cuts at: 3071 3098 3125 5070 1678 Size: 27 27 1945 2531 Fragments arranged by size: 2531 1945 1150 81 27 27 27 27 27 27 27 27 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4564 4596 4638 4670 1373 Size: 32 135 32 2992 32 42 32 2626 Fragments arranged by size: 2992 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3267 3513 669 Size: 988 281 1329 246 3079 Fragments arranged by size: 3079 1329 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5923 HpaI GTT'AAC Cuts at: 3513 3513 Size: 5923 KasI G'GCGC_C Cuts at: 2824 2851 2878 2905 2932 2959 3040 3067 3094 Size: 27 27 27 27 27 81 27 27 Cuts at: 3094 3121 2824 Size: 27 5626 Fragments arranged by size: 5626 81 27 27 27 27 27 27 27 27 KpnI G_GTAC'C Cuts at: 3248 3248 Size: 5923 MfeI C'AATT_G Cuts at: 3522 3522 Size: 5923 MluI A'CGCG_T Cuts at: 5799 5799 Size: 5923 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5037 5221 1305 Size: 3732 184 2007 Fragments arranged by size: 3732 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4101 Fragments arranged by size: 4101 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3881 4240 4399 518 Size: 648 2715 359 159 2042 Fragments arranged by size: 2715 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2393 2455 3339 4016 5164 5409 1606 Size: 787 62 884 677 1148 245 2120 Fragments arranged by size: 2120 1148 884 787 677 245 62 NaeI GCC'GGC Cuts at: 2373 2373 Size: 5923 NarI GG'CG_CC Cuts at: 2825 2852 2879 2906 2933 2960 3041 3068 3095 Size: 27 27 27 27 27 81 27 27 Cuts at: 3095 3122 2825 Size: 27 5626 Fragments arranged by size: 5626 81 27 27 27 27 27 27 27 27 NcoI C'CATG_G Cuts at: 513 1065 2004 2775 2820 2901 2928 2955 2982 Size: 552 939 771 45 81 27 27 27 Cuts at: 2982 3009 3036 3063 3090 3117 513 Size: 27 27 27 27 27 3319 Fragments arranged by size: 3319 939 771 552 81 45 27 27 27 27 27 27 27 27 NdeI CA'TA_TG Cuts at: 387 387 Size: 5923 NgoMIV G'CCGG_C Cuts at: 2371 2371 Size: 5923 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5923 NotI GC'GGCC_GC Cuts at: 3370 3370 Size: 5923 NsiI A_TGCA'T Cuts at: 3211 3211 Size: 5923 NspI r_CATG'y Cuts at: 1132 1229 2656 3146 3213 3281 4826 1132 Size: 97 1427 490 67 68 1545 2229 Fragments arranged by size: 2229 1545 1427 490 97 68 67 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5923 PciI A'CATG_T Cuts at: 2652 3142 4822 2652 Size: 490 1680 3753 Fragments arranged by size: 3753 1680 490 PflMI CCAn_nnn'nTGG Cuts at: 1108 2647 1108 Size: 1539 4384 PmeI GTTT'AAAC Cuts at: 3227 3227 Size: 5923 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3992 4024 5505 5537 3992 Size: 32 1481 32 4378 Fragments arranged by size: 4378 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5923 PsiI TTA'TAA Cuts at: 3493 3493 Size: 5923 PspOMI G'GGCC_C Cuts at: 3183 3183 Size: 5923 PspXI vC'TCGA_Gb Cuts at: 2315 2315 Size: 5923 PstI C_TGCA'G Cuts at: 830 2460 3275 830 Size: 1630 815 3478 Fragments arranged by size: 3478 1630 815 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5923 PvuII CAG'CTG Cuts at: 2455 2455 Size: 5923 SalI G'TCGA_C Cuts at: 1936 3265 1936 Size: 1329 4594 SapI GCTCTTCn'nnn_ Cuts at: 2569 2569 Size: 5923 SbfI CC_TGCA'GG Cuts at: 3275 3275 Size: 5923 ScaI AGT'ACT Cuts at: 4171 5883 4171 Size: 1712 4211 SfcI C'TryA_G Cuts at: 826 1045 2456 3271 4406 5087 5278 826 Size: 219 1411 815 1135 681 191 1471 Fragments arranged by size: 1471 1411 1135 815 681 219 191 SfoI GGC'GCC Cuts at: 2826 2853 2880 2907 2934 2961 3042 3069 3096 Size: 27 27 27 27 27 81 27 27 Cuts at: 3096 3123 2826 Size: 27 5626 Fragments arranged by size: 5626 81 27 27 27 27 27 27 27 27 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5923 SmaI CCC'GGG Cuts at: 1872 2866 2974 3001 3028 3055 3082 3109 3250 Size: 994 108 27 27 27 27 27 141 Cuts at: 3250 1872 Size: 4545 Fragments arranged by size: 4545 994 141 108 27 27 27 27 27 SmlI C'TyrA_G Cuts at: 820 1017 1943 2315 4027 4928 5190 820 Size: 197 926 372 1712 901 262 1553 Fragments arranged by size: 1712 1553 926 901 372 262 197 SnaBI TAC'GTA Cuts at: 493 3714 493 Size: 3221 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5923 SphI G_CATG'C Cuts at: 1132 3281 1132 Size: 2149 3774 SspI AAT'ATT Cuts at: 5 52 3847 5 Size: 47 3795 2081 Fragments arranged by size: 3795 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5923 StyI C'CwwG_G Cuts at: 513 1065 2004 2775 2820 2901 2928 2955 2982 Size: 552 939 771 45 81 27 27 27 Cuts at: 2982 3009 3036 3063 3090 3117 3362 513 Size: 27 27 27 27 27 245 3074 Fragments arranged by size: 3074 939 771 552 245 81 45 27 27 27 27 27 27 27 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2042 2651 2779 2788 2806 2815 2833 Size: 1255 462 609 128 9 18 9 18 Cuts at: 2833 3130 3148 3950 3967 4120 4305 4750 325 Size: 297 18 802 17 153 185 445 1498 Fragments arranged by size: 1498 1255 802 609 462 445 297 185 153 128 18 18 18 17 9 9 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3169 4169 5881 Size: 274 80 33 51 1443 1192 1000 1712 Cuts at: 5881 96 Size: 138 Fragments arranged by size: 1712 1443 1192 1000 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2763 2795 5883 5915 2763 Size: 32 3088 32 2771 Fragments arranged by size: 3088 2771 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5923 XbaI T'CTAG_A Cuts at: 3259 3259 Size: 5923 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2036 2773 2036 Size: 737 5186 XhoI C'TCGA_G Cuts at: 1943 2315 1943 Size: 372 5551 XmaI C'CCGG_G Cuts at: 1870 2864 2972 2999 3026 3053 3080 3107 3248 Size: 994 108 27 27 27 27 27 141 Cuts at: 3248 1870 Size: 4545 Fragments arranged by size: 4545 994 141 108 27 27 27 27 27 XmnI GAAnn'nnTTC Cuts at: 2474 4052 2474 Size: 1578 4345 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3731 276 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI PacI PfoI PmlI PpuMI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI