(Linear) (Six-base) MAPSORT of: KIBB7678.seq Check: 5777 from: 1 to: 1218 KIBB7678.orf With 182 enzymes: SgfI * July 3, 2012 16:26 .. AclI AA'CG_TT Cuts at: 0 179 1218 Size: 179 1039 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 337 557 1218 Size: 337 220 661 Fragments arranged by size: 661 337 220 AflIII A'CryG_T Cuts at: 0 647 1137 1218 Size: 647 490 81 Fragments arranged by size: 647 490 81 ApaI G_GGCC'C Cuts at: 0 1182 1218 Size: 1182 36 ApoI r'AATT_y Cuts at: 0 528 1218 Size: 528 690 AvaI C'yCGr_G Cuts at: 0 310 859 967 994 1021 1048 1075 1102 Size: 310 549 108 27 27 27 27 27 Cuts at: 1102 1218 Size: 116 Fragments arranged by size: 549 310 116 108 27 27 27 27 27 BamHI G'GATC_C Cuts at: 0 687 1218 Size: 687 531 BanI G'GyrC_C Cuts at: 0 711 819 846 873 900 927 954 1035 Size: 711 108 27 27 27 27 27 81 Cuts at: 1035 1062 1089 1116 1218 Size: 27 27 27 102 Fragments arranged by size: 711 108 102 81 27 27 27 27 27 27 27 27 BanII G_rGCy'C Cuts at: 0 1182 1218 Size: 1182 36 BbeI G_GCGC'C Cuts at: 0 823 850 877 904 931 958 1039 1066 Size: 823 27 27 27 27 27 81 27 Cuts at: 1066 1093 1120 1218 Size: 27 27 98 Fragments arranged by size: 823 98 81 27 27 27 27 27 27 27 27 BbsI GAAGACnn'nnnn_ Cuts at: 0 545 1218 Size: 545 673 BbvCI CC'TCA_GC Cuts at: 0 389 1218 Size: 389 829 BfrBI ATG'CAT Cuts at: 0 1204 1218 Size: 1204 14 BglI GCCn_nnn'nGGC Cuts at: 0 762 789 861 888 915 942 969 996 Size: 762 27 72 27 27 27 27 27 Cuts at: 996 1023 1050 1077 1104 1218 Size: 27 27 27 27 114 Fragments arranged by size: 762 114 72 27 27 27 27 27 27 27 27 27 27 Bme1580I G_kGCm'C Cuts at: 0 1182 1218 Size: 1182 36 Bpu10I CC'TnA_GC Cuts at: 0 389 1218 Size: 389 829 BsaHI Gr'CG_yC Cuts at: 0 820 847 874 901 928 955 1036 1063 Size: 820 27 27 27 27 27 81 27 Cuts at: 1063 1090 1117 1218 Size: 27 27 101 Fragments arranged by size: 820 101 81 27 27 27 27 27 27 27 27 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 134 145 274 310 346 1218 Size: 134 11 129 36 36 872 Fragments arranged by size: 872 134 129 36 36 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 568 1218 Size: 568 650 BsiEI CG_ry'CG Cuts at: 0 350 1218 Size: 350 868 Bsp1286I G_dGCh'C Cuts at: 0 1182 1218 Size: 1182 36 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 540 1218 Size: 540 678 BsrFI r'CCGG_y Cuts at: 0 366 1218 Size: 366 852 BsrGI T'GTAC_A Cuts at: 0 1164 1218 Size: 1164 54 BssSI C'ACGA_G Cuts at: 0 238 1218 Size: 238 980 BstYI r'GATC_y Cuts at: 0 687 1218 Size: 687 531 BtgI C'CryG_G Cuts at: 0 350 770 815 896 923 950 977 1004 Size: 350 420 45 81 27 27 27 27 Cuts at: 1004 1031 1058 1085 1112 1218 Size: 27 27 27 27 106 Fragments arranged by size: 420 350 106 81 45 27 27 27 27 27 27 27 27 BtsI GCAGTG_nn' Cuts at: 0 614 1218 Size: 614 604 EaeI y'GGCC_r Cuts at: 0 234 889 916 1218 Size: 234 655 27 302 Fragments arranged by size: 655 302 234 27 EarI CTCTTCn'nnn_ Cuts at: 0 14 564 1218 Size: 14 550 654 Fragments arranged by size: 654 550 14 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 385 1218 Size: 385 833 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 337 557 1218 Size: 337 220 661 Fragments arranged by size: 661 337 220 EcoO109I rG'GnC_Cy Cuts at: 0 662 1179 1218 Size: 662 517 39 Fragments arranged by size: 662 517 39 HaeII r_GCGC'y Cuts at: 0 823 850 877 904 931 958 1039 1066 Size: 823 27 27 27 27 27 81 27 Cuts at: 1066 1093 1120 1218 Size: 27 27 98 Fragments arranged by size: 823 98 81 27 27 27 27 27 27 27 27 KasI G'GCGC_C Cuts at: 0 819 846 873 900 927 954 1035 1062 Size: 819 27 27 27 27 27 81 27 Cuts at: 1062 1089 1116 1218 Size: 27 27 102 Fragments arranged by size: 819 102 81 27 27 27 27 27 27 27 27 MspA1I CmG'CkG Cuts at: 0 388 450 1218 Size: 388 62 768 Fragments arranged by size: 768 388 62 NaeI GCC'GGC Cuts at: 0 368 1218 Size: 368 850 NarI GG'CG_CC Cuts at: 0 820 847 874 901 928 955 1036 1063 Size: 820 27 27 27 27 27 81 27 Cuts at: 1063 1090 1117 1218 Size: 27 27 101 Fragments arranged by size: 820 101 81 27 27 27 27 27 27 27 27 NcoI C'CATG_G Cuts at: 0 770 815 896 923 950 977 1004 1031 Size: 770 45 81 27 27 27 27 27 Cuts at: 1031 1058 1085 1112 1218 Size: 27 27 27 106 Fragments arranged by size: 770 106 81 45 27 27 27 27 27 27 27 27 NgoMIV G'CCGG_C Cuts at: 0 366 1218 Size: 366 852 NsiI A_TGCA'T Cuts at: 0 1206 1218 Size: 1206 12 NspI r_CATG'y Cuts at: 0 651 1141 1208 1218 Size: 651 490 67 10 Fragments arranged by size: 651 490 67 10 PciI A'CATG_T Cuts at: 0 647 1137 1218 Size: 647 490 81 Fragments arranged by size: 647 490 81 PflMI CCAn_nnn'nTGG Cuts at: 0 642 1218 Size: 642 576 PspOMI G'GGCC_C Cuts at: 0 1178 1218 Size: 1178 40 PspXI vC'TCGA_Gb Cuts at: 0 310 1218 Size: 310 908 PstI C_TGCA'G Cuts at: 0 455 1218 Size: 455 763 PvuII CAG'CTG Cuts at: 0 450 1218 Size: 450 768 SapI GCTCTTCn'nnn_ Cuts at: 0 564 1218 Size: 564 654 SfcI C'TryA_G Cuts at: 0 451 1218 Size: 451 767 SfoI GGC'GCC Cuts at: 0 821 848 875 902 929 956 1037 1064 Size: 821 27 27 27 27 27 81 27 Cuts at: 1064 1091 1118 1218 Size: 27 27 100 Fragments arranged by size: 821 100 81 27 27 27 27 27 27 27 27 SmaI CCC'GGG Cuts at: 0 861 969 996 1023 1050 1077 1104 1218 Size: 861 108 27 27 27 27 27 114 Fragments arranged by size: 861 114 108 27 27 27 27 27 SmlI C'TyrA_G Cuts at: 0 310 1218 Size: 310 908 StyI C'CwwG_G Cuts at: 0 770 815 896 923 950 977 1004 1031 Size: 770 45 81 27 27 27 27 27 Cuts at: 1031 1058 1085 1112 1218 Size: 27 27 27 106 Fragments arranged by size: 770 106 81 45 27 27 27 27 27 27 27 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 37 646 774 783 801 810 828 1125 Size: 37 609 128 9 18 9 18 297 Cuts at: 1125 1143 1218 Size: 18 75 Fragments arranged by size: 609 297 128 75 37 18 18 18 9 9 TatI w'GTAC_w Cuts at: 0 1164 1218 Size: 1164 54 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 758 790 1218 Size: 758 32 428 Fragments arranged by size: 758 428 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 31 768 1218 Size: 31 737 450 Fragments arranged by size: 737 450 31 XhoI C'TCGA_G Cuts at: 0 310 1218 Size: 310 908 XmaI C'CCGG_G Cuts at: 0 859 967 994 1021 1048 1075 1102 1218 Size: 859 108 27 27 27 27 27 116 Fragments arranged by size: 859 116 108 27 27 27 27 27 XmnI GAAnn'nnTTC Cuts at: 0 469 1218 Size: 469 749 Enzymes that do cut: AclI AcuI AflIII ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BfrBI BglI Bme1580I Bpu10I BsaHI BseRI BsgI BsiEI Bsp1286I BspMI BsrFI BsrGI BssSI BstYI BtgI BtsI EaeI EarI EciI Eco57MI EcoO109I HaeII KasI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI PflMI PspOMI PspXI PstI PvuII SapI SfcI SfoI SmaI SmlI StyI TaqII TatI TstI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI BglII BlpI BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseYI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI NdeI NheI NotI NruI PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI ZraI