Restriction map for
pFN21AE4344
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# NO CUT ENZYMES ( 136 enzymes) #
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AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BseRI
BsgI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I CelII Cfr10I ClaI
CpoI DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoRI EcoT22I FauI FbaI
FseI FspI HindIII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MscI MslI MunI
NaeI NarI NcoI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tsp45I
Tth111I TthHB8I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccII 263 CGCG CG^CG
AccIII 1013 TCCGGA T^CCGGA
AcsI 766 AAATTT [GA]^AATT[CT]
AcyI 241 GACGCC G[GA]^CG[CT]C
Alw44I 500 GTGCAC G^TGCAC
AlwNI 130 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 292 CCTCAGG CC^T[ACGT]AGG
ApaLI 500 GTGCAC G^TGCAC
ApoI 766 AAATTT [GA]^AATT[CT]
Asp700I 763 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 500 GTGCAC G[AT]GC[AT]^C
BamHI 325 GGATCC G^GATCC
BanII 888 GAGCCC G[GA]GC[CT]^C
BglI 244 GCCTTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 241 GACGCC G[GA]^CG[CT]C
BsaI 661 GAGACC GGTCTC(1/5)
BsaWI 1013 TCCGGA [AT]^CCGG[AT]
BseAI 1013 TCCGGA T^CCGGA
BsiHKAI 500 GTGCAC G[AT]GC[AT]^C
BsmFI 182 GGGAC GGGAC(10/14)
BsmI 808 GAATGC GAATGC(1/-1)
BspEI 1013 TCCGGA T^CCGGA
BspHI 818 TCATGA T^CATGA
BsrDI 176 GCAATG GCAATG(2/0)
BstEII 543 GGTTACC G^GT[ACGT]ACC
BstPI 543 GGTTACC G^GT[ACGT]ACC
BstUI 263 CGCG CG^CG
BstXI 650 CCATCAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 325 GGATCC [GA]^GATC[CT]
Bsu36I 292 CCTCAGG CC^T[ACGT]AGG
DraI 397 TTTAAA TTT^AAA
DraII 1025 AGGACCT [GA]G^G[ACGT]CC[CT]
Eco81I 292 CCTCAGG CC^T[ACGT]AGG
EcoNI 288 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1025 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 543 GGTTACC G^GT[ACGT]ACC
EcoRV 637 GATATC GAT^ATC
HaeII 94 AGCGCC [GA]GCGC^[CT]
Hin1I 241 GACGCC G[GA]^CG[CT]C
HincII 442 GTCAAC GT[CT]^[GA]AC
HindII 442 GTCAAC GT[CT]^[GA]AC
MflI 325 GGATCC [GA]^GATC[CT]
MroI 1013 TCCGGA T^CCGGA
MseI 398 TTAA T^TAA
MvnI 263 CGCG CG^CG
NheI 487 GCTAGC G^CTAGC
NspI 50 ACATGC [GA]CATG^[CT]
PflMI 477 CCAGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1025 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 818 TCATGA T^CATGA
SfaNI 856 GCATC GCATC(5/9)
SfcI 102 CTGTAG C^T[GA][CT]AG
TfiI 1072 GATTC G^A[AT]TC
Tru9I 398 TTAA T^TAA
Tsp509I 767 AATT ^AATT
Van91I 477 CCAGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 717 CCATGATTTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 325 GGATCC [GA]^GATC[CT]
XmnI 763 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
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AlwI 326 GATCC GGATC(4/5)
325 GGATC
BbsI 603 GAAGAC GAAGAC(2/6)
873 GAAGAC
BcnI 62 CCCGG CC^[GC]GG
665 CCGGG
BfaI 80 CTAG C^TAG
488 CTAG
BmyI 500 GTGCAC G[AGT]GC[ACT]^C
888 GAGCCC
BpuAI 603 GAAGAC GAAGAC(2/6)
873 GAAGAC
Bsp1286I 500 GTGCAC G[AGT]GC[ACT]^C
888 GAGCCC
BspMI 287 ACCTGC ACCTGC(4/8)
1034 ACCTGC
EcoT14I 482 CCTTGG C^C[AT][AT]GG
1046 CCTTGG
HaeIII 507 GGCC GG^CC
847 GGCC
HgaI 241 GACGC GACGC(5/10)
261 GACGC
MaeI 80 CTAG C^TAG
488 CTAG
MaeII 165 ACGT A^CGT
446 ACGT
MspA1I 842 CAGCTG C[AC]G^C[GT]G
920 CAGCTG
NciI 62 CCCGG CC^[GC]GG
665 CCGGG
PvuII 842 CAGCTG CAG^CTG
920 CAGCTG
StyI 482 CCTTGG C^C[AT][AT]GG
1046 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BpmI BsaJI BsiYI BslI BsmAI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI TseI
TspRI