Restriction map for
pFN21AE4344
(Circular) (Six-base) MAPSORT of: pFN21AE4344.seq Check: 7818 from: 1 to: 5785
pFN21AE4344 5785 bp
With 182 enzymes: SgfI *
July 6, 2012 17:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3595 278
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3128 1937
Size: 1191 4594
Acc65I G'GTAC_C
Cuts at: 3106 3106
Size: 5785
AclI AA'CG_TT
Cuts at: 1563 3912 4285 1563
Size: 2349 373 3063
Fragments arranged by size:
3063 2349 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2868 3848 5232 5500 1632
Size: 1236 980 1384 268 1917
Fragments arranged by size:
1917 1384 1236 980 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5588
AflIII A'CryG_T
Cuts at: 1203 4684 5661 1203
Size: 3481 977 1327
Fragments arranged by size:
3481 1327 977
AgeI A'CCGG_T
Cuts at: 4616 4616
Size: 5785
AhdI GACnn_n'nnGTC
Cuts at: 4514 4514
Size: 5785
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5785
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5785
AlwNI CAG_nnn'CTG
Cuts at: 2140 3528 5100 2140
Size: 1388 1572 2825
Fragments arranged by size:
2825 1572 1388
ApaLI G'TGCA_C
Cuts at: 2505 3842 4998 2505
Size: 1337 1156 3292
Fragments arranged by size:
3292 1337 1156
ApoI r'AATT_y
Cuts at: 1493 2771 3094 3320 3472 5581 1493
Size: 1278 323 226 152 2109 1697
Fragments arranged by size:
2109 1697 1278 323 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4339 160
Size: 872 3307 1606
Fragments arranged by size:
3307 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
AvaI C'yCGr_G
Cuts at: 1870 1943 3110 1870
Size: 73 1167 4545
Fragments arranged by size:
4545 1167 73
BamHI G'GATC_C
Cuts at: 2330 3115 3482 2330
Size: 785 367 4633
Fragments arranged by size:
4633 785 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3106 4561 618
Size: 325 217 627 1319 1455 1842
Fragments arranged by size:
1842 1455 1319 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2897 3104 1391
Size: 1506 207 4072
Fragments arranged by size:
4072 1506 207
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2615 2885 928
Size: 971 716 270 3828
Fragments arranged by size:
3828 971 716 270
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3976 4010 1903
Size: 34 2039 34 3678
Fragments arranged by size:
3678 2039 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3677 4887 3677
Size: 1210 4575
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5785
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2255 4396 136
Size: 107 122 71 631 605 583 2141 1525
Fragments arranged by size:
2141 1525 631 605 583 122 107 71
BglII A'GATC_T
Cuts at: 5780 5780
Size: 5785
BlpI GC'TnA_GC
Cuts at: 3202 3202
Size: 5785
Bme1580I G_kGCm'C
Cuts at: 1790 2509 3846 5002 1790
Size: 719 1337 1156 2573
Fragments arranged by size:
2573 1337 1156 719
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5785
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2476 3516 4474
Size: 416 53 83 399 24 1059 1040 958
Cuts at: 4474 442
Size: 1753
Fragments arranged by size:
1753 1059 1040 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2496 1056
Size: 1440 4345
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2381 2684 2907 2932 4445 1239
Size: 1142 303 223 25 1513 2579
Fragments arranged by size:
2579 1513 1142 303 223 25
Bpu10I CC'TnA_GC
Cuts at: 2784 2784
Size: 5785
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3910 4775 5073 3910
Size: 865 298 4622
Fragments arranged by size:
4622 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2660 4448 882
Size: 617 1161 1788 2219
Fragments arranged by size:
2219 1788 1161 617
BsaAI yAC'GTr
Cuts at: 493 1204 3576 493
Size: 711 2372 2702
Fragments arranged by size:
2702 2372 711
BsaBI GATnn'nnATC
Cuts at: 5779 5779
Size: 5785
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2247 3592 3974 4609
Size: 53 83 186 936 714 1345 382 635
Cuts at: 4609 275
Size: 1451
Fragments arranged by size:
1451 1345 936 714 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3018 4218 4616 4890 5037 5448 3018
Size: 1200 398 274 147 411 3355
Fragments arranged by size:
3355 1200 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2930 2960 1178
Size: 30 165 30 1527 30 4003
Fragments arranged by size:
4003 1527 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5785
BseYI C'CCAG_C
Cuts at: 4988 5484 4988
Size: 496 5289
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5212
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3235 3996 5024 1540
Size: 275 186 1234 761 1028 2301
Fragments arranged by size:
2301 1234 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2509 3846 3931 5002 2509
Size: 1337 85 1071 3292
Fragments arranged by size:
3292 1337 1071 85
BsmI GAATG_Cn'
Cuts at: 2819 3296 3389 2819
Size: 477 93 5215
Fragments arranged by size:
5215 477 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2509 2897 3104 3846 3931 5002 1391
Size: 399 719 388 207 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 719 399 388 207 85
BspEI T'CCGG_A
Cuts at: 3018 5448 3018
Size: 2430 3355
BspHI T'CATG_A
Cuts at: 2823 3674 5404 2823
Size: 851 1730 3204
Fragments arranged by size:
3204 1730 851
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2301 3048 3140 844
Size: 1457 747 92 3489
Fragments arranged by size:
3489 1457 747 92
BsrBI CCG'CTC
Cuts at: 1394 1823 3672 1394
Size: 429 1849 3507
Fragments arranged by size:
3507 1849 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2188 4280 4454 65
Size: 1183 940 2092 174 1396
Fragments arranged by size:
2092 1396 1183 940 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4429 4616 1700
Size: 110 2619 187 2869
Fragments arranged by size:
2869 2619 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5785
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5785
BssSI C'ACGA_G
Cuts at: 3845 4857 3845
Size: 1012 4773
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5785
BstBI TT'CG_AA
Cuts at: 4589 4589
Size: 5785
BstEII G'GTnAC_C
Cuts at: 1184 2548 1184
Size: 1364 4421
BstXI CCAn_nnnn'nTGG
Cuts at: 2662 2662
Size: 5785
BstYI r'GATC_y
Cuts at: 1972 2330 3115 3482 3867 3884 5336 5422 5434
Size: 358 785 367 385 17 1452 86 12
Cuts at: 5434 5780 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 785 385 367 358 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2298 2298
Size: 5785
BtgI C'CryG_G
Cuts at: 513 1065 2004 3081 513
Size: 552 939 1077 3217
Fragments arranged by size:
3217 1077 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5046
Fragments arranged by size:
5046 683 56
BtsI GCAGTG_nn'
Cuts at: 2569 3301 4095 4115 2569
Size: 732 794 20 4239
Fragments arranged by size:
4239 794 732 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5785
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5750
DraI TTT'AAA
Cuts at: 2404 3089 2404
Size: 685 5100
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4792 809
Size: 507 3476 1802
Fragments arranged by size:
3476 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3232 4121 5750
Size: 54 1425 50 275 18 1402 889 1629
Cuts at: 5750 8
Size: 43
Fragments arranged by size:
1629 1425 1402 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3232 1537
Size: 275 1420 4090
Fragments arranged by size:
4090 1420 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3715 1353
Size: 2362 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4372 4746 4892 387
Size: 1064 138 2783 374 146 1280
Fragments arranged by size:
2783 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2381 2684 2868 2907 2932 3848 4445
Size: 393 749 303 184 39 25 916 597
Cuts at: 4445 5232 5500 1239
Size: 787 268 1524
Fragments arranged by size:
1524 916 787 749 597 393 303 268
184 39 25
EcoNI CCTnn'n_nnAGG
Cuts at: 2297 2297
Size: 5785
EcoO109I rG'GnC_Cy
Cuts at: 3031 3031
Size: 5785
EcoRI G'AATT_C
Cuts at: 3094 3472 3094
Size: 378 5407
EcoRV GAT'ATC
Cuts at: 2644 2644
Size: 5785
FspI TGC'GCA
Cuts at: 4291 4291
Size: 5785
HaeII r_GCGC'y
Cuts at: 1678 2103 4932 1678
Size: 425 2829 2531
Fragments arranged by size:
2829 2531 425
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2030 2062 2257 2289 2884
Size: 32 135 32 458 32 195 32 595
Cuts at: 2884 2916 3050 3082 4426 4458 4500 4532 1373
Size: 32 134 32 1344 32 42 32 2626
Fragments arranged by size:
2626 1344 595 458 195 135 134 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2449 3129 3375 669
Size: 988 281 511 680 246 3079
Fragments arranged by size:
3079 988 680 511 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5785
HpaI GTT'AAC
Cuts at: 3375 3375
Size: 5785
KpnI G_GTAC'C
Cuts at: 3110 3110
Size: 5785
MfeI C'AATT_G
Cuts at: 3384 3384
Size: 5785
MluI A'CGCG_T
Cuts at: 5661 5661
Size: 5785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4899 5083 1305
Size: 3594 184 2007
Fragments arranged by size:
3594 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3963
Fragments arranged by size:
3963 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3743 4102 4261 518
Size: 648 2577 359 159 2042
Fragments arranged by size:
2577 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2849 2927 3201 3878 5026 5271 1606
Size: 1243 78 274 677 1148 245 2120
Fragments arranged by size:
2120 1243 1148 677 274 245 78
NcoI C'CATG_G
Cuts at: 513 1065 2004 3081 513
Size: 552 939 1077 3217
Fragments arranged by size:
3217 1077 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5785
NheI G'CTAG_C
Cuts at: 1052 2492 1052
Size: 1440 4345
NotI GC'GGCC_GC
Cuts at: 3232 3232
Size: 5785
NspI r_CATG'y
Cuts at: 1132 1229 2059 3143 4688 1132
Size: 97 830 1084 1545 2229
Fragments arranged by size:
2229 1545 1084 830 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5785
PciI A'CATG_T
Cuts at: 4684 4684
Size: 5785
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2488 1108
Size: 1380 4405
PfoI T'CCnGG_A
Cuts at: 2048 2066 2333 2048
Size: 18 267 5500
Fragments arranged by size:
5500 267 18
PmeI GTTT'AAAC
Cuts at: 3089 3089
Size: 5785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3854 3886 5367 5399 3854
Size: 32 1481 32 4240
Fragments arranged by size:
4240 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3031 3031
Size: 5785
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5785
PsiI TTA'TAA
Cuts at: 3355 3355
Size: 5785
PstI C_TGCA'G
Cuts at: 830 3137 830
Size: 2307 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5785
PvuII CAG'CTG
Cuts at: 2849 2927 2849
Size: 78 5707
SalI G'TCGA_C
Cuts at: 1936 3127 1936
Size: 1191 4594
SbfI CC_TGCA'GG
Cuts at: 3137 3137
Size: 5785
ScaI AGT'ACT
Cuts at: 4033 5745 4033
Size: 1712 4073
SfcI C'TryA_G
Cuts at: 826 1045 2107 3133 4268 4949 5140 826
Size: 219 1062 1026 1135 681 191 1471
Fragments arranged by size:
1471 1135 1062 1026 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5785
SmaI CCC'GGG
Cuts at: 1872 3112 1872
Size: 1240 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3889 4790 5052 820
Size: 197 926 1946 901 262 1553
Fragments arranged by size:
1946 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3576 493
Size: 3083 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5785
SphI G_CATG'C
Cuts at: 1132 3143 1132
Size: 2011 3774
SspI AAT'ATT
Cuts at: 5 52 3709 5
Size: 47 3657 2081
Fragments arranged by size:
3657 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5785
StyI C'CwwG_G
Cuts at: 513 1065 2004 2487 3051 3081 3224 513
Size: 552 939 483 564 30 143 3074
Fragments arranged by size:
3074 939 564 552 483 143 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3812 3829 3982 4167 4612 325
Size: 1255 2232 17 153 185 445 1498
Fragments arranged by size:
2232 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2415 4031 5743
Size: 274 80 33 51 1443 438 1616 1712
Cuts at: 5743 96
Size: 138
Fragments arranged by size:
1712 1616 1443 438 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5745 5777 5745
Size: 32 5753
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5785
XbaI T'CTAG_A
Cuts at: 3121 3121
Size: 5785
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2729 2729
Size: 5785
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5785
XmaI C'CCGG_G
Cuts at: 1870 3110 1870
Size: 1240 4545
XmnI GAAnn'nnTTC
Cuts at: 2772 3914 2772
Size: 1142 4643
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3593 276
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI