(Circular) (Six-base) MAPSORT of: pFN21AE3232.seq Check: 1550 from: 1 to: 6325 pFN21AE3232 6325 bp With 182 enzymes: SgfI * July 6, 2012 17:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3202 3202 Size: 6325 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4135 278 Size: 53 83 186 936 2599 2468 Fragments arranged by size: 2599 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2613 3668 1937 Size: 676 1055 4594 Fragments arranged by size: 4594 1055 676 Acc65I G'GTAC_C Cuts at: 3646 3646 Size: 6325 AclI AA'CG_TT Cuts at: 1563 4452 4825 1563 Size: 2889 373 3063 Fragments arranged by size: 3063 2889 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2329 4388 5772 6040 1632 Size: 697 2059 1384 268 1917 Fragments arranged by size: 2059 1917 1384 697 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6128 AflIII A'CryG_T Cuts at: 1203 2694 3069 5224 6201 1203 Size: 1491 375 2155 977 1327 Fragments arranged by size: 2155 1491 1327 977 375 AgeI A'CCGG_T Cuts at: 2075 5156 2075 Size: 3081 3244 AhdI GACnn_n'nnGTC Cuts at: 5054 5054 Size: 6325 AleI CACnn'nnGTG Cuts at: 1166 2216 2531 1166 Size: 1050 315 4960 Fragments arranged by size: 4960 1050 315 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6325 AlwNI CAG_nnn'CTG Cuts at: 3623 4068 5640 3623 Size: 445 1572 4308 Fragments arranged by size: 4308 1572 445 ApaI G_GGCC'C Cuts at: 3365 3365 Size: 6325 ApaLI G'TGCA_C Cuts at: 4382 5538 4382 Size: 1156 5169 ApoI r'AATT_y Cuts at: 1493 3634 3860 4012 6121 1493 Size: 2141 226 152 2109 1697 Fragments arranged by size: 2141 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4879 160 Size: 872 3847 1606 Fragments arranged by size: 3847 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6325 AvaI C'yCGr_G Cuts at: 1870 1943 2214 2654 3650 1870 Size: 73 271 440 996 4545 Fragments arranged by size: 4545 996 440 271 73 BamHI G'GATC_C Cuts at: 3655 4022 3655 Size: 367 5958 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2072 2378 2536 3284 3646 Size: 325 217 627 285 306 158 748 362 Cuts at: 3646 5101 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 748 627 362 325 306 285 217 158 BanII G_rGCy'C Cuts at: 1391 2877 3048 3365 3644 1391 Size: 1486 171 317 279 4072 Fragments arranged by size: 4072 1486 317 279 171 BbeI G_GCGC'C Cuts at: 2382 2540 2382 Size: 158 6167 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2665 2745 928 Size: 971 766 80 4508 Fragments arranged by size: 4508 971 766 80 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4516 4550 1903 Size: 34 50 34 2495 34 3678 Fragments arranged by size: 3678 2495 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4217 5427 4217 Size: 1210 5115 BclI T'GATC_A Cuts at: 1548 2778 1548 Size: 1230 5095 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4936 136 Size: 107 122 71 631 605 3264 1525 Fragments arranged by size: 3264 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6320 6320 Size: 6325 BlpI GC'TnA_GC Cuts at: 2940 3742 2940 Size: 802 5523 Bme1580I G_kGCm'C Cuts at: 1790 2294 3287 3365 4386 5542 1790 Size: 504 993 78 1021 1156 2573 Fragments arranged by size: 2573 1156 1021 993 504 78 BmgBI CAC'GTC Cuts at: 1318 2697 1318 Size: 1379 4946 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2277 2461 4056 Size: 416 53 83 399 24 860 184 1595 Cuts at: 4056 5014 442 Size: 958 1753 Fragments arranged by size: 1753 1595 958 860 416 399 184 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6325 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2614 2651 2945 4985 1239 Size: 1375 37 294 2040 2579 Fragments arranged by size: 2579 2040 1375 294 37 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4450 5315 5613 4450 Size: 865 298 5162 Fragments arranged by size: 5162 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2711 3228 4988 882 Size: 617 1212 517 1760 2219 Fragments arranged by size: 2219 1760 1212 617 517 BsaAI yAC'GTr Cuts at: 493 1204 4116 493 Size: 711 2912 2702 Fragments arranged by size: 2912 2702 711 BsaBI GATnn'nnATC Cuts at: 6319 6319 Size: 6325 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2128 2379 2537 4132 Size: 53 83 186 936 595 251 158 1595 Cuts at: 4132 4514 5149 275 Size: 382 635 1451 Fragments arranged by size: 1595 1451 936 635 595 382 251 186 158 83 53 BsaWI w'CCGG_w Cuts at: 2075 2805 4758 5156 5430 5577 5988 2075 Size: 730 1953 398 274 147 411 2412 Fragments arranged by size: 2412 1953 730 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6100 Fragments arranged by size: 6100 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2696 2931 3092 1186 Size: 1510 235 161 4419 Fragments arranged by size: 4419 1510 235 161 BseYI C'CCAG_C Cuts at: 2052 2474 3549 5528 6024 2052 Size: 422 1075 1979 496 2353 Fragments arranged by size: 2353 1979 1075 496 422 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2589 1163 Size: 573 853 4899 Fragments arranged by size: 4899 853 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2225 3341 3775 4536 5564 1540 Size: 275 186 224 1116 434 761 1028 2301 Fragments arranged by size: 2301 1116 1028 761 434 275 224 186 BsiHKAI G_wGCw'C Cuts at: 2025 2877 4386 4471 5542 2025 Size: 852 1509 85 1071 2808 Fragments arranged by size: 2808 1509 1071 852 85 BsmI GAATG_Cn' Cuts at: 3836 3929 3836 Size: 93 6232 BsmBI CGTCTCn'nnnn_ Cuts at: 2016 2016 Size: 6325 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2294 2877 3048 3287 3365 3644 Size: 399 235 269 583 171 239 78 279 Cuts at: 3644 4386 4471 5542 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 583 399 279 269 239 235 171 85 78 BspEI T'CCGG_A Cuts at: 2805 5988 2805 Size: 3183 3142 BspHI T'CATG_A Cuts at: 4214 5944 4214 Size: 1730 4595 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2466 3202 3680 844 Size: 1622 736 478 3489 Fragments arranged by size: 3489 1622 736 478 BsrBI CCG'CTC Cuts at: 1394 1823 2635 4212 1394 Size: 429 812 1577 3507 Fragments arranged by size: 3507 1577 812 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4820 4994 65 Size: 1183 3572 174 1396 Fragments arranged by size: 3572 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2066 2075 2224 4969 5156 1700 Size: 110 256 9 149 2745 187 2869 Fragments arranged by size: 2869 2745 256 187 149 110 9 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6325 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6325 BssSI C'ACGA_G Cuts at: 2666 4385 5397 2666 Size: 1719 1012 3594 Fragments arranged by size: 3594 1719 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6325 BstBI TT'CG_AA Cuts at: 5129 5129 Size: 6325 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6325 BstXI CCAn_nnnn'nTGG Cuts at: 2728 2728 Size: 6325 BstYI r'GATC_y Cuts at: 1972 2731 3605 3655 4022 4407 4424 5876 5962 Size: 759 874 50 367 385 17 1452 86 Cuts at: 5962 5974 6320 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 874 759 385 367 346 86 50 17 12 BtgI C'CryG_G Cuts at: 513 1065 3012 513 Size: 552 1947 3826 Fragments arranged by size: 3826 1947 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2430 487 Size: 683 56 1204 4382 Fragments arranged by size: 4382 1204 683 56 BtsI GCAGTG_nn' Cuts at: 2800 3456 3841 4635 4655 2800 Size: 656 385 794 20 4470 Fragments arranged by size: 4470 794 656 385 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6325 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3412 3447 529 Size: 35 2848 35 3407 Fragments arranged by size: 3407 2848 35 35 DraI TTT'AAA Cuts at: 3369 3629 3369 Size: 260 6065 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5332 809 Size: 507 4016 1802 Fragments arranged by size: 4016 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2064 2222 2226 Size: 54 1425 50 275 18 234 158 4 Cuts at: 2226 2484 3338 3772 4661 6290 8 Size: 258 854 434 889 1629 43 Fragments arranged by size: 1629 1425 889 854 434 275 258 234 158 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2222 3338 3772 1537 Size: 275 410 1116 434 4090 Fragments arranged by size: 4090 1116 434 410 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2348 2882 2893 2906 4255 1353 Size: 995 534 11 13 1349 3423 Fragments arranged by size: 3423 1349 995 534 13 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2139 2570 4912 5286 5432 387 Size: 1064 138 550 431 2342 374 146 1280 Fragments arranged by size: 2342 1280 1064 550 431 374 146 138 EcoICRI GAG'CTC Cuts at: 2875 2875 Size: 6325 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2329 2614 2651 2945 4388 4985 5772 Size: 393 697 285 37 294 1443 597 787 Cuts at: 5772 6040 1239 Size: 268 1524 Fragments arranged by size: 1524 1443 787 697 597 393 294 285 268 37 EcoO109I rG'GnC_Cy Cuts at: 2455 3361 3362 2455 Size: 906 1 5418 Fragments arranged by size: 5418 906 1 EcoRI G'AATT_C Cuts at: 3634 4012 3634 Size: 378 5947 FseI GG_CCGG'CC Cuts at: 2228 2228 Size: 6325 FspI TGC'GCA Cuts at: 2246 2750 4831 2246 Size: 504 2081 3740 Fragments arranged by size: 3740 2081 504 HaeII r_GCGC'y Cuts at: 1678 2147 2382 2429 2483 2540 5472 1678 Size: 469 235 47 54 57 2932 2531 Fragments arranged by size: 2932 2531 469 235 57 54 47 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2763 2795 2956 2988 4966 Size: 32 135 32 1191 32 161 32 1978 Cuts at: 4966 4998 5040 5072 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1978 1191 161 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3067 3669 3915 669 Size: 988 281 1129 602 246 3079 Fragments arranged by size: 3079 1129 988 602 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6325 HpaI GTT'AAC Cuts at: 3067 3915 3067 Size: 848 5477 KasI G'GCGC_C Cuts at: 2378 2536 2378 Size: 158 6167 KpnI G_GTAC'C Cuts at: 3650 3650 Size: 6325 MfeI C'AATT_G Cuts at: 3924 3924 Size: 6325 MluI A'CGCG_T Cuts at: 6201 6201 Size: 6325 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5439 5623 1305 Size: 4134 184 2007 Fragments arranged by size: 4134 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4503 Fragments arranged by size: 4503 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2216 2531 2819 2832 3017 4283 4642 Size: 648 1050 315 288 13 185 1266 359 Cuts at: 4642 4801 518 Size: 159 2042 Fragments arranged by size: 2042 1266 1050 648 359 315 288 185 159 13 MspA1I CmG'CkG Cuts at: 1606 2489 3741 4418 5566 5811 1606 Size: 883 1252 677 1148 245 2120 Fragments arranged by size: 2120 1252 1148 883 677 245 NaeI GCC'GGC Cuts at: 2068 2226 2068 Size: 158 6167 NarI GG'CG_CC Cuts at: 2379 2537 2379 Size: 158 6167 NcoI C'CATG_G Cuts at: 513 1065 3012 513 Size: 552 1947 3826 Fragments arranged by size: 3826 1947 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6325 NgoMIV G'CCGG_C Cuts at: 2066 2224 2066 Size: 158 6167 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6325 NotI GC'GGCC_GC Cuts at: 3772 3772 Size: 6325 NspI r_CATG'y Cuts at: 1132 1229 3073 3683 5228 1132 Size: 97 1844 610 1545 2229 Fragments arranged by size: 2229 1844 1545 610 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6325 PciI A'CATG_T Cuts at: 3069 5224 3069 Size: 2155 4170 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6325 PfoI T'CCnGG_A Cuts at: 2676 2676 Size: 6325 PmeI GTTT'AAAC Cuts at: 3629 3629 Size: 6325 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4394 4426 5907 5939 4394 Size: 32 1481 32 4780 Fragments arranged by size: 4780 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2455 2455 Size: 6325 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6325 PsiI TTA'TAA Cuts at: 3895 3895 Size: 6325 PspOMI G'GGCC_C Cuts at: 3361 3361 Size: 6325 PspXI vC'TCGA_Gb Cuts at: 2654 2654 Size: 6325 PstI C_TGCA'G Cuts at: 830 3677 830 Size: 2847 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6325 SacI G_AGCT'C Cuts at: 2877 2877 Size: 6325 SalI G'TCGA_C Cuts at: 1936 3667 1936 Size: 1731 4594 SapI GCTCTTCn'nnn_ Cuts at: 2348 2882 2893 2348 Size: 534 11 5780 Fragments arranged by size: 5780 534 11 SbfI CC_TGCA'GG Cuts at: 3677 3677 Size: 6325 ScaI AGT'ACT Cuts at: 2516 3129 4573 6285 2516 Size: 613 1444 1712 2556 Fragments arranged by size: 2556 1712 1444 613 SfcI C'TryA_G Cuts at: 826 1045 2614 2704 3673 4808 5489 5680 826 Size: 219 1569 90 969 1135 681 191 1471 Fragments arranged by size: 1569 1471 1135 969 681 219 191 90 SfoI GGC'GCC Cuts at: 2380 2538 2380 Size: 158 6167 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6325 SgrAI Cr'CCGG_yG Cuts at: 1700 2075 1700 Size: 375 5950 SmaI CCC'GGG Cuts at: 1872 2216 3652 1872 Size: 344 1436 4545 Fragments arranged by size: 4545 1436 344 SmlI C'TyrA_G Cuts at: 820 1017 1943 2654 4429 5330 5592 820 Size: 197 926 711 1775 901 262 1553 Fragments arranged by size: 1775 1553 926 901 711 262 197 SnaBI TAC'GTA Cuts at: 493 4116 493 Size: 3623 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6325 SphI G_CATG'C Cuts at: 1132 3683 1132 Size: 2551 3774 SspI AAT'ATT Cuts at: 5 52 4249 5 Size: 47 4197 2081 Fragments arranged by size: 4197 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6325 StyI C'CwwG_G Cuts at: 513 1065 3012 3356 3568 3764 513 Size: 552 1947 344 212 196 3074 Fragments arranged by size: 3074 1947 552 344 212 196 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3252 3302 4352 4369 4522 4707 5152 Size: 1255 1672 50 1050 17 153 185 445 Cuts at: 5152 325 Size: 1498 Fragments arranged by size: 1672 1498 1255 1050 445 185 153 50 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2514 3127 4571 Size: 274 80 33 51 1443 537 613 1444 Cuts at: 4571 6283 96 Size: 1712 138 Fragments arranged by size: 1712 1444 1443 613 537 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3450 3482 6285 6317 3450 Size: 32 2803 32 3458 Fragments arranged by size: 3458 2803 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6325 XbaI T'CTAG_A Cuts at: 3661 3661 Size: 6325 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2770 3271 3296 2770 Size: 501 25 5799 Fragments arranged by size: 5799 501 25 XhoI C'TCGA_G Cuts at: 1943 2654 1943 Size: 711 5614 XmaI C'CCGG_G Cuts at: 1870 2214 3650 1870 Size: 344 1436 4545 Fragments arranged by size: 4545 1436 344 XmnI GAAnn'nnTTC Cuts at: 4454 4454 Size: 6325 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4133 276 Size: 53 83 186 936 2599 2468 Fragments arranged by size: 2599 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FspAI NruI NsiI PacI PmlI PsrI PvuII RsrII SacII SanDI SexAI SfiI SrfI SwaI