(Linear) (Six-base) MAPSORT of: KIEE3232.seq Check: 9668 from: 1 to: 1620 KIEE3232.orf With 182 enzymes: SgfI * July 3, 2012 17:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1197 1620 Size: 1197 423 AccI GT'mk_AC Cuts at: 0 608 1620 Size: 608 1012 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 324 1620 Size: 324 1296 AflIII A'CryG_T Cuts at: 0 689 1064 1620 Size: 689 375 556 Fragments arranged by size: 689 556 375 AgeI A'CCGG_T Cuts at: 0 70 1620 Size: 70 1550 AleI CACnn'nnGTG Cuts at: 0 211 526 1620 Size: 211 315 1094 Fragments arranged by size: 1094 315 211 ApaI G_GGCC'C Cuts at: 0 1360 1620 Size: 1360 260 AvaI C'yCGr_G Cuts at: 0 209 649 1620 Size: 209 440 971 Fragments arranged by size: 971 440 209 BanI G'GyrC_C Cuts at: 0 67 373 531 1279 1620 Size: 67 306 158 748 341 Fragments arranged by size: 748 341 306 158 67 BanII G_rGCy'C Cuts at: 0 872 1043 1360 1620 Size: 872 171 317 260 Fragments arranged by size: 872 317 260 171 BbeI G_GCGC'C Cuts at: 0 377 535 1620 Size: 377 158 1085 Fragments arranged by size: 1085 377 158 BbsI GAAGACnn'nnnn_ Cuts at: 0 660 740 1620 Size: 660 80 880 Fragments arranged by size: 880 660 80 BclI T'GATC_A Cuts at: 0 773 1620 Size: 773 847 BlpI GC'TnA_GC Cuts at: 0 935 1620 Size: 935 685 Bme1580I G_kGCm'C Cuts at: 0 289 1282 1360 1620 Size: 289 993 78 260 Fragments arranged by size: 993 289 260 78 BmgBI CAC'GTC Cuts at: 0 692 1620 Size: 692 928 BmrI ACTGGGnnnn_n' Cuts at: 0 272 456 1620 Size: 272 184 1164 Fragments arranged by size: 1164 272 184 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 609 646 940 1620 Size: 609 37 294 680 Fragments arranged by size: 680 609 294 37 BsaI GGTCTCn'nnnn_ Cuts at: 0 706 1223 1620 Size: 706 517 397 Fragments arranged by size: 706 517 397 BsaHI Gr'CG_yC Cuts at: 0 123 374 532 1620 Size: 123 251 158 1088 Fragments arranged by size: 1088 251 158 123 BsaWI w'CCGG_w Cuts at: 0 70 800 1620 Size: 70 730 820 Fragments arranged by size: 820 730 70 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 691 926 1087 1620 Size: 691 235 161 533 Fragments arranged by size: 691 533 235 161 BseYI C'CCAG_C Cuts at: 0 47 469 1544 1620 Size: 47 422 1075 76 Fragments arranged by size: 1075 422 76 47 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 584 1620 Size: 584 1036 BsiEI CG_ry'CG Cuts at: 0 220 1336 1620 Size: 220 1116 284 Fragments arranged by size: 1116 284 220 BsiHKAI G_wGCw'C Cuts at: 0 20 872 1620 Size: 20 852 748 Fragments arranged by size: 852 748 20 BsmBI CGTCTCn'nnnn_ Cuts at: 0 11 1620 Size: 11 1609 Bsp1286I G_dGCh'C Cuts at: 0 20 289 872 1043 1282 1360 1620 Size: 20 269 583 171 239 78 260 Fragments arranged by size: 583 269 260 239 171 78 20 BspEI T'CCGG_A Cuts at: 0 800 1620 Size: 800 820 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 461 1197 1620 Size: 461 736 423 Fragments arranged by size: 736 461 423 BsrBI CCG'CTC Cuts at: 0 630 1620 Size: 630 990 BsrFI r'CCGG_y Cuts at: 0 61 70 219 1620 Size: 61 9 149 1401 Fragments arranged by size: 1401 149 61 9 BssSI C'ACGA_G Cuts at: 0 661 1620 Size: 661 959 BstXI CCAn_nnnn'nTGG Cuts at: 0 723 1620 Size: 723 897 BstYI r'GATC_y Cuts at: 0 726 1600 1620 Size: 726 874 20 Fragments arranged by size: 874 726 20 BtgI C'CryG_G Cuts at: 0 1007 1620 Size: 1007 613 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 425 1620 Size: 425 1195 BtsI GCAGTG_nn' Cuts at: 0 795 1451 1620 Size: 795 656 169 Fragments arranged by size: 795 656 169 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1407 1442 1620 Size: 1407 35 178 Fragments arranged by size: 1407 178 35 DraI TTT'AAA Cuts at: 0 1364 1620 Size: 1364 256 EaeI y'GGCC_r Cuts at: 0 59 217 221 479 1333 1620 Size: 59 158 4 258 854 287 Fragments arranged by size: 854 287 258 158 59 4 EagI C'GGCC_G Cuts at: 0 217 1333 1620 Size: 217 1116 287 Fragments arranged by size: 1116 287 217 EarI CTCTTCn'nnn_ Cuts at: 0 343 877 888 901 1620 Size: 343 534 11 13 719 Fragments arranged by size: 719 534 343 13 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 134 565 1620 Size: 134 431 1055 Fragments arranged by size: 1055 431 134 EcoICRI GAG'CTC Cuts at: 0 870 1620 Size: 870 750 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 324 609 646 940 1620 Size: 324 285 37 294 680 Fragments arranged by size: 680 324 294 285 37 EcoO109I rG'GnC_Cy Cuts at: 0 450 1356 1357 1620 Size: 450 906 1 263 Fragments arranged by size: 906 450 263 1 FseI GG_CCGG'CC Cuts at: 0 223 1620 Size: 223 1397 FspI TGC'GCA Cuts at: 0 241 745 1620 Size: 241 504 875 Fragments arranged by size: 875 504 241 HaeII r_GCGC'y Cuts at: 0 142 377 424 478 535 1620 Size: 142 235 47 54 57 1085 Fragments arranged by size: 1085 235 142 57 54 47 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 758 790 951 983 1620 Size: 758 32 161 32 637 Fragments arranged by size: 758 637 161 32 32 HincII GTy'rAC Cuts at: 0 1062 1620 Size: 1062 558 HpaI GTT'AAC Cuts at: 0 1062 1620 Size: 1062 558 KasI G'GCGC_C Cuts at: 0 373 531 1620 Size: 373 158 1089 Fragments arranged by size: 1089 373 158 MslI CAynn'nnrTG Cuts at: 0 211 526 814 827 1012 1620 Size: 211 315 288 13 185 608 Fragments arranged by size: 608 315 288 211 185 13 MspA1I CmG'CkG Cuts at: 0 484 1620 Size: 484 1136 NaeI GCC'GGC Cuts at: 0 63 221 1620 Size: 63 158 1399 Fragments arranged by size: 1399 158 63 NarI GG'CG_CC Cuts at: 0 374 532 1620 Size: 374 158 1088 Fragments arranged by size: 1088 374 158 NcoI C'CATG_G Cuts at: 0 1007 1620 Size: 1007 613 NgoMIV G'CCGG_C Cuts at: 0 61 219 1620 Size: 61 158 1401 Fragments arranged by size: 1401 158 61 NspI r_CATG'y Cuts at: 0 1068 1620 Size: 1068 552 PciI A'CATG_T Cuts at: 0 1064 1620 Size: 1064 556 PfoI T'CCnGG_A Cuts at: 0 671 1620 Size: 671 949 PpuMI rG'GwC_Cy Cuts at: 0 450 1620 Size: 450 1170 PspOMI G'GGCC_C Cuts at: 0 1356 1620 Size: 1356 264 PspXI vC'TCGA_Gb Cuts at: 0 649 1620 Size: 649 971 SacI G_AGCT'C Cuts at: 0 872 1620 Size: 872 748 SapI GCTCTTCn'nnn_ Cuts at: 0 343 877 888 1620 Size: 343 534 11 732 Fragments arranged by size: 732 534 343 11 ScaI AGT'ACT Cuts at: 0 511 1124 1620 Size: 511 613 496 Fragments arranged by size: 613 511 496 SfcI C'TryA_G Cuts at: 0 609 699 1620 Size: 609 90 921 Fragments arranged by size: 921 609 90 SfoI GGC'GCC Cuts at: 0 375 533 1620 Size: 375 158 1087 Fragments arranged by size: 1087 375 158 SgrAI Cr'CCGG_yG Cuts at: 0 70 1620 Size: 70 1550 SmaI CCC'GGG Cuts at: 0 211 1620 Size: 211 1409 SmlI C'TyrA_G Cuts at: 0 649 1620 Size: 649 971 StyI C'CwwG_G Cuts at: 0 1007 1351 1563 1620 Size: 1007 344 212 57 Fragments arranged by size: 1007 344 212 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1247 1297 1620 Size: 1247 50 323 Fragments arranged by size: 1247 323 50 TatI w'GTAC_w Cuts at: 0 509 1122 1620 Size: 509 613 498 Fragments arranged by size: 613 509 498 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1445 1477 1620 Size: 1445 32 143 Fragments arranged by size: 1445 143 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 765 1266 1291 1620 Size: 765 501 25 329 Fragments arranged by size: 765 501 329 25 XhoI C'TCGA_G Cuts at: 0 649 1620 Size: 649 971 XmaI C'CCGG_G Cuts at: 0 209 1620 Size: 209 1411 Enzymes that do cut: AarI AccI AcuI AflIII AgeI AleI ApaI AvaI BanI BanII BbeI BbsI BclI BlpI Bme1580I BmgBI BmrI BpmI BsaI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstXI BstYI BtgI BtgZI BtsI CspCI DraI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FseI FspI HaeII Hin4I HincII HpaI KasI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI PpuMI PspOMI PspXI SacI SapI ScaI SfcI SfoI SgrAI SmaI SmlI StyI TaqII TatI TstI XcmI XhoI XmaI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AhdI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BfrBI BglI BglII BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaXI BsiWI BsmI BspHI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I ClaI DraIII DrdI EcoNI EcoRI EcoRV FalI FspAI HindIII KpnI MfeI MluI MmeI MscI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmeI PmlI PpiI PshAI PsiI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SbfI SexAI SfiI SgfI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XmnI ZraI