Restriction map for
pFN21AB7995
##################################
# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BglI BlnI
BlpI Bpu1102I BsaBI BsaI BseAI
BseRI BsiEI BsiWI BsmBI BspDI
BspEI BspMI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV FauI FbaI FseI
FspI HaeII HhaI HinP1I HincII
HindII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MroI
MslI MunI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SpeI SphI
Sse8387I SwaI Tth111I Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AciI 1302 GCGG CCGC(-3/-1)
AcyI 385 GGCGTC G[GA]^CG[CT]C
AflII 889 CTTAAG C^TTAAG
AflIII 227 ACATGT A^C[GA][CT]GT
AlwI 753 GGATC GGATC(4/5)
AseI 522 ATTAAT AT^TAAT
AsnI 522 ATTAAT AT^TAAT
Asp700I 799 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 64 GTGCTC G[AT]GC[AT]^C
AvaI 512 CCCGGG C^[CT]CG[GA]G
AvaII 708 GGACC G^G[AT]CC
BanI 861 GGCACC G^G[CT][GA]CC
BanII 764 GAGCCC G[GA]GC[CT]^C
BfrI 889 CTTAAG C^TTAAG
BsaAI 106 TACGTA [CT]AC^GT[GA]
BsaHI 385 GGCGTC G[GA]^CG[CT]C
BsiHKAI 64 GTGCTC G[AT]GC[AT]^C
BsmFI 707 GGGAC GGGAC(10/14)
BsoBI 512 CCCGGG C^[CT]CG[GA]G
Bsp1407I 230 TGTACA T^GTACA
BspHI 389 TCATGA T^CATGA
BspLU11I 227 ACATGT A^CATGT
BsrGI 230 TGTACA T^GTACA
EcoNI 531 CCTTATTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 386 GCGTC GACGC(5/10)
Hin1I 385 GGCGTC G[GA]^CG[CT]C
MaeIII 34 GTGAC ^GT[ACGT]AC
MspA1I 654 CAGCTG C[AC]G^C[GT]G
NdeI 791 CATATG CA^TATG
PpuMI 707 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 522 ATTAAT AT^TAAT
PstI 218 CTGCAG CTGCA^G
PvuII 654 CAGCTG CAG^CTG
RcaI 389 TCATGA T^CATGA
SexAI 568 ACCTGGT A^CC[AT]GGT
SfcI 218 CTGCAG C^T[GA][CT]AG
SmaI 512 CCCGGG CCC^GGG
SmlI 889 CTTAAG C^T[CT][GA]AG
SnaBI 106 TACGTA TAC^GTA
SspBI 230 TGTACA T^GTACA
StuI 539 AGGCCT AGG^CCT
TaqI 756 TCGA T^CGA
TfiI 256 GATTC G^A[AT]TC
Tsp45I 34 GTGAC ^GT[GC]AC
TthHB8I 756 TCGA T^CGA
XcmI 332 CCAAAAGCAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 512 CCCGGG C^CCGGG
XmaI 512 CCCGGG C^CCGGG
XmnI 799 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
BalI 119 TGGCCA TGG^CCA
1135 TGGCCA
BbsI 660 GAAGAC GAAGAC(2/6)
507 GTCTTC
BcnI 512 CCCGG CC^[GC]GG
513 CCGGG
BglII 404 AGATCT A^GATCT
1312 AGATCT
BmyI 64 GTGCTC G[AGT]GC[ACT]^C
764 GAGCCC
BpmI 1119 CTCCAG CTGGAG(16/14)
698 CTGGAG
BpuAI 660 GAAGAC GAAGAC(2/6)
507 GTCTTC
BsaWI 368 ACCGGA [AT]^CCGG[AT]
1253 TCCGGT
BsgI 281 CTGCAC GTGCAG(16/14)
74 GTGCAG
BsmI 80 GAATGC GAATGC(1/-1)
443 GAATGC
Bsp1286I 64 GTGCTC G[AGT]GC[ACT]^C
764 GAGCCC
BsrI 981 ACTGG ACTGG(1/-1)
1130 ACTGG
BstYI 404 AGATCT [GA]^GATC[CT]
1312 AGATCT
Cac8I 213 GCAAGC GC[ACGT]^[ACGT]GC
276 GCAGGC
Cfr13I 28 GGGCC G^G[ACGT]CC
708 GGACC
DraI 399 TTTAAA TTT^AAA
1263 TTTAAA
DraII 27 AGGGCCT [GA]G^G[ACGT]CC[CT]
707 GGGACCC
EaeI 119 TGGCCA [CT]^GGCC[GA]
1135 TGGCCA
EcoO109I 27 AGGGCCT [GA]G^G[ACGT]CC[CT]
707 GGGACCC
EcoRI 742 GAATTC G^AATTC
934 GAATTC
EcoT14I 91 CCTTGG C^C[AT][AT]GG
248 CCTTGG
HindIII 598 AAGCTT A^AGCTT
1208 AAGCTT
MaeII 107 ACGT A^CGT
505 ACGT
MflI 404 AGATCT [GA]^GATC[CT]
1312 AGATCT
MluNI 119 TGGCCA TGG^CCA
1135 TGGCCA
MscI 119 TGGCCA TGG^CCA
1135 TGGCCA
NciI 512 CCCGG CC^[GC]GG
513 CCGGG
NspI 227 ACATGT [GA]CATG^[CT]
417 ACATGC
PleI 1346 GACTC GAGTC(4/5)
1004 GAGTC
Sau96I 28 GGGCC G^G[ACGT]CC
708 GGACC
StyI 91 CCTTGG C^C[AT][AT]GG
248 CCTTGG
TspRI 12 CAGTG CA[GC]TG[ACGT][ACGT]^
69 CAGTG
XhoII 404 AGATCT [GA]^GATC[CT]
1312 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BsaJI BsiYI BslI BsmAI BssKI
BstF5I BstNI DdeI DpnII EarI
EcoRII EcoT22I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MboI MboII MnlI MseI
MspI MvaI MwoI NdeII NlaIII
NlaIV NsiI RsaI Sau3AI ScrFI
SfaNI SspI Tru9I TseI Tsp509I