Restriction map for
pFN21AB7995
(Linear) (Six-base) MAPSORT of: KIBB7995.seq Check: 9572 from: 1 to: 1350
KIBB7995.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 1350
Size: 217 1133
AflII C'TTAA_G
Cuts at: 0 889 1350
Size: 889 461
AflIII A'CryG_T
Cuts at: 0 227 1350
Size: 227 1123
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 233 1350
Size: 233 1117
ApoI r'AATT_y
Cuts at: 0 314 692 742 934 1350
Size: 314 378 50 192 416
Fragments arranged by size:
416 378 314 192 50
AseI AT'TA_AT
Cuts at: 0 523 1350
Size: 523 827
AvaI C'yCGr_G
Cuts at: 0 512 1350
Size: 512 838
BanI G'GyrC_C
Cuts at: 0 861 1350
Size: 861 489
BanII G_rGCy'C
Cuts at: 0 768 1350
Size: 768 582
BbsI GAAGACnn'nnnn_
Cuts at: 0 500 667 1350
Size: 500 167 683
Fragments arranged by size:
683 500 167
BfrBI ATG'CAT
Cuts at: 0 84 421 790 1350
Size: 84 337 369 560
Fragments arranged by size:
560 369 337 84
BglII A'GATC_T
Cuts at: 0 404 1312 1350
Size: 404 908 38
Fragments arranged by size:
908 404 38
BmrI ACTGGGnnnn_n'
Cuts at: 0 991 1350
Size: 991 359
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1134 1350
Size: 1134 216
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 719 1104 1350
Size: 719 385 246
Fragments arranged by size:
719 385 246
BsaAI yAC'GTr
Cuts at: 0 108 1350
Size: 108 1242
BsaHI Gr'CG_yC
Cuts at: 0 386 1350
Size: 386 964
BsaWI w'CCGG_w
Cuts at: 0 368 1253 1350
Size: 368 885 97
Fragments arranged by size:
885 368 97
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 480 510 1350
Size: 480 30 840
Fragments arranged by size:
840 480 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 266 1350
Size: 95 171 1084
Fragments arranged by size:
1084 171 95
BsiHKAI G_wGCw'C
Cuts at: 0 68 1350
Size: 68 1282
BsmI GAATG_Cn'
Cuts at: 0 86 449 1350
Size: 86 363 901
Fragments arranged by size:
901 363 86
Bsp1286I G_dGCh'C
Cuts at: 0 68 768 1350
Size: 68 700 582
Fragments arranged by size:
700 582 68
BspHI T'CATG_A
Cuts at: 0 389 1350
Size: 389 961
BsrGI T'GTAC_A
Cuts at: 0 230 1350
Size: 230 1120
BstYI r'GATC_y
Cuts at: 0 404 1312 1350
Size: 404 908 38
Fragments arranged by size:
908 404 38
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1093 1128 1350
Size: 1093 35 222
Fragments arranged by size:
1093 222 35
DraI TTT'AAA
Cuts at: 0 401 1265 1350
Size: 401 864 85
Fragments arranged by size:
864 401 85
EaeI y'GGCC_r
Cuts at: 0 119 1135 1350
Size: 119 1016 215
Fragments arranged by size:
1016 215 119
EarI CTCTTCn'nnn_
Cuts at: 0 715 1103 1303 1350
Size: 715 388 200 47
Fragments arranged by size:
715 388 200 47
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 719 1104 1350
Size: 217 502 385 246
Fragments arranged by size:
502 385 246 217
EcoNI CCTnn'n_nnAGG
Cuts at: 0 535 1350
Size: 535 815
EcoO109I rG'GnC_Cy
Cuts at: 0 28 708 1350
Size: 28 680 642
Fragments arranged by size:
680 642 28
EcoRI G'AATT_C
Cuts at: 0 742 934 1350
Size: 742 192 416
Fragments arranged by size:
742 416 192
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 426 1350
Size: 426 924
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 885 917 1350
Size: 885 32 433
Fragments arranged by size:
885 433 32
HindIII A'AGCT_T
Cuts at: 0 598 1208 1350
Size: 598 610 142
Fragments arranged by size:
610 598 142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 911 968 1350
Size: 911 57 382
Fragments arranged by size:
911 382 57
MscI TGG'CCA
Cuts at: 0 121 1137 1350
Size: 121 1016 213
Fragments arranged by size:
1016 213 121
MspA1I CmG'CkG
Cuts at: 0 656 1350
Size: 656 694
NdeI CA'TA_TG
Cuts at: 0 792 1350
Size: 792 558
NsiI A_TGCA'T
Cuts at: 0 86 423 792 1350
Size: 86 337 369 558
Fragments arranged by size:
558 369 337 86
NspI r_CATG'y
Cuts at: 0 231 421 1350
Size: 231 190 929
Fragments arranged by size:
929 231 190
PciI A'CATG_T
Cuts at: 0 227 1350
Size: 227 1123
PfoI T'CCnGG_A
Cuts at: 0 696 1120 1350
Size: 696 424 230
Fragments arranged by size:
696 424 230
PpuMI rG'GwC_Cy
Cuts at: 0 708 1350
Size: 708 642
PstI C_TGCA'G
Cuts at: 0 222 1350
Size: 222 1128
PvuII CAG'CTG
Cuts at: 0 656 1350
Size: 656 694
SanDI GG'GwC_CC
Cuts at: 0 708 1350
Size: 708 642
SexAI A'CCwGG_T
Cuts at: 0 568 1350
Size: 568 782
SfcI C'TryA_G
Cuts at: 0 218 1350
Size: 218 1132
SmaI CCC'GGG
Cuts at: 0 514 1350
Size: 514 836
SmlI C'TyrA_G
Cuts at: 0 889 1350
Size: 889 461
SnaBI TAC'GTA
Cuts at: 0 108 1350
Size: 108 1242
SspI AAT'ATT
Cuts at: 0 381 521 675 1350
Size: 381 140 154 675
Fragments arranged by size:
675 381 154 140
StuI AGG'CCT
Cuts at: 0 541 1350
Size: 541 809
StyI C'CwwG_G
Cuts at: 0 91 248 1350
Size: 91 157 1102
Fragments arranged by size:
1102 157 91
TatI w'GTAC_w
Cuts at: 0 230 1350
Size: 230 1120
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 480 512 1350
Size: 480 32 838
Fragments arranged by size:
838 480 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 339 1350
Size: 339 1011
XmaI C'CCGG_G
Cuts at: 0 512 1350
Size: 512 838
XmnI GAAnn'nnTTC
Cuts at: 0 803 1350
Size: 803 547
Enzymes that do cut:
AcuI AflII AflIII AlfI ApoI AseI AvaI BanI
BanII BbsI BfrBI BglII BmrI BplI BpmI BsaAI
BsaHI BsaWI BsaXI BsgI BsiHKAI BsmI Bsp1286I BspHI
BsrGI BstYI CspCI DraI EaeI EarI Eco57MI EcoNI
EcoO109I EcoRI FalI Hin4I HindIII MmeI MscI MspA1I
NdeI NsiI NspI PciI PfoI PpuMI PstI PvuII
SanDI SexAI SfcI SmaI SmlI SnaBI SspI StuI
StyI TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AloI AlwNI ApaI ApaLI AscI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BglI
BlpI Bme1580I BmgBI BmtI Bpu10I BpuEI BsaI BsaBI
BseRI BseYI BsiEI BsiWI BsmBI BspEI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraIII DrdI
EagI EciI EcoICRI EcoRV FseI FspI FspAI HaeII
HincII HpaI KasI KpnI MfeI MluI MslI NaeI
NarI NcoI NgoMIV NheI NotI NruI PacI PasI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SpeI SphI SrfI
SwaI TaqII Tth111I XbaI XhoI ZraI