(Circular) (Six-base) MAPSORT of: pFN21AB7765.seq Check: 9804 from: 1 to: 6055 pFN21AB7765 6055 bp With 182 enzymes: SgfI * July 6, 2012 17:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3865 278 Size: 53 83 186 936 2329 2468 Fragments arranged by size: 2468 2329 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2544 3398 1937 Size: 607 854 4594 Fragments arranged by size: 4594 854 607 Acc65I G'GTAC_C Cuts at: 3376 3376 Size: 6055 AclI AA'CG_TT Cuts at: 1563 4182 4555 1563 Size: 2619 373 3063 Fragments arranged by size: 3063 2619 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2760 4118 5502 5770 1632 Size: 1128 1358 1384 268 1917 Fragments arranged by size: 1917 1384 1358 1128 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5858 AflIII A'CryG_T Cuts at: 1203 4954 5931 1203 Size: 3751 977 1327 Fragments arranged by size: 3751 1327 977 AgeI A'CCGG_T Cuts at: 4886 4886 Size: 6055 AhdI GACnn_n'nnGTC Cuts at: 4784 4784 Size: 6055 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6055 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6055 AlwNI CAG_nnn'CTG Cuts at: 2203 2557 2687 2738 3076 3798 5370 2203 Size: 354 130 51 338 722 1572 2888 Fragments arranged by size: 2888 1572 722 354 338 130 51 ApaLI G'TGCA_C Cuts at: 4112 5268 4112 Size: 1156 4899 ApoI r'AATT_y Cuts at: 1493 2975 3086 3364 3590 3742 5851 1493 Size: 1482 111 278 226 152 2109 1697 Fragments arranged by size: 2109 1697 1482 278 226 152 111 AseI AT'TA_AT Cuts at: 160 1032 4609 160 Size: 872 3577 1606 Fragments arranged by size: 3577 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 AvaI C'yCGr_G Cuts at: 1870 1943 3380 1870 Size: 73 1437 4545 Fragments arranged by size: 4545 1437 73 BamHI G'GATC_C Cuts at: 3385 3752 3385 Size: 367 5688 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2284 2361 3376 4831 618 Size: 325 217 627 497 77 1015 1455 1842 Fragments arranged by size: 1842 1455 1015 627 497 325 217 77 BanII G_rGCy'C Cuts at: 1391 2505 3374 1391 Size: 1114 869 4072 Fragments arranged by size: 4072 1114 869 BbeI G_GCGC'C Cuts at: 2288 2365 2288 Size: 77 5978 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2665 928 Size: 971 766 4318 Fragments arranged by size: 4318 971 766 BbvCI CC'TCA_GC Cuts at: 2705 2705 Size: 6055 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4246 4280 1903 Size: 34 2309 34 3678 Fragments arranged by size: 3678 2309 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3947 5157 3947 Size: 1210 4845 BclI T'GATC_A Cuts at: 1548 1548 Size: 6055 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2370 4666 136 Size: 107 122 71 631 605 698 2296 1525 Fragments arranged by size: 2296 1525 698 631 605 122 107 71 BglII A'GATC_T Cuts at: 6050 6050 Size: 6055 BlpI GC'TnA_GC Cuts at: 3472 3472 Size: 6055 Bme1580I G_kGCm'C Cuts at: 1790 4116 5272 1790 Size: 2326 1156 2573 Fragments arranged by size: 2573 2326 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6055 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2209 2798 3137 Size: 416 53 83 399 24 792 589 339 Cuts at: 3137 3786 4744 442 Size: 649 958 1753 Fragments arranged by size: 1753 958 792 649 589 416 399 339 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6055 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2816 2816 Size: 6055 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2172 2417 3315 3350 4715 1239 Size: 933 245 898 35 1365 2579 Fragments arranged by size: 2579 1365 933 898 245 35 Bpu10I CC'TnA_GC Cuts at: 2705 2705 Size: 6055 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4180 5045 5343 4180 Size: 865 298 4892 Fragments arranged by size: 4892 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3291 4718 882 Size: 617 1792 1427 2219 Fragments arranged by size: 2219 1792 1427 617 BsaAI yAC'GTr Cuts at: 493 1204 3846 493 Size: 711 2642 2702 Fragments arranged by size: 2702 2642 711 BsaBI GATnn'nnATC Cuts at: 6049 6049 Size: 6055 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2285 2362 3862 4244 Size: 53 83 186 936 752 77 1500 382 Cuts at: 4244 4879 275 Size: 635 1451 Fragments arranged by size: 1500 1451 936 752 635 382 186 83 77 53 BsaWI w'CCGG_w Cuts at: 3111 4488 4886 5160 5307 5718 3111 Size: 1377 398 274 147 411 3448 Fragments arranged by size: 3448 1377 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2087 2117 2423 2453 1178 Size: 30 165 30 684 30 306 30 4780 Fragments arranged by size: 4780 684 306 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2092 2693 1186 Size: 906 601 4548 Fragments arranged by size: 4548 906 601 BseYI C'CCAG_C Cuts at: 3069 5258 5754 3069 Size: 2189 496 3370 Fragments arranged by size: 3370 2189 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2258 2549 2555 1163 Size: 573 522 291 6 4663 Fragments arranged by size: 4663 573 522 291 6 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2223 2320 2370 2877 3505 4266 Size: 275 186 222 97 50 507 628 761 Cuts at: 4266 5294 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 628 507 275 222 186 97 50 BsiHKAI G_wGCw'C Cuts at: 4116 4201 5272 4116 Size: 85 1071 4899 Fragments arranged by size: 4899 1071 85 BsiWI C'GTAC_G Cuts at: 2250 2250 Size: 6055 BsmI GAATG_Cn' Cuts at: 2774 3566 3659 2774 Size: 792 93 5170 Fragments arranged by size: 5170 792 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3146 3146 Size: 6055 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2505 3374 4116 4201 5272 1391 Size: 399 715 869 742 85 1071 2174 Fragments arranged by size: 2174 1071 869 742 715 399 85 BspEI T'CCGG_A Cuts at: 5718 5718 Size: 6055 BspHI T'CATG_A Cuts at: 3944 5674 3944 Size: 1730 4325 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2454 3410 844 Size: 1610 956 3489 Fragments arranged by size: 3489 1610 956 BsrBI CCG'CTC Cuts at: 1394 1823 2339 2439 2894 3942 1394 Size: 429 516 100 455 1048 3507 Fragments arranged by size: 3507 1048 516 455 429 100 BsrDI GCAATG_nn' Cuts at: 65 1248 2080 4550 4724 65 Size: 1183 832 2470 174 1396 Fragments arranged by size: 2470 1396 1183 832 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2281 2369 2829 4699 4886 1700 Size: 110 471 88 460 1870 187 2869 Fragments arranged by size: 2869 1870 471 460 187 110 88 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6055 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6055 BssSI C'ACGA_G Cuts at: 4115 5127 4115 Size: 1012 5043 BstAPI GCAn_nnn'nTGC Cuts at: 794 2560 794 Size: 1766 4289 BstBI TT'CG_AA Cuts at: 4859 4859 Size: 6055 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6055 BstXI CCAn_nnnn'nTGG Cuts at: 3203 3203 Size: 6055 BstYI r'GATC_y Cuts at: 1972 3385 3752 4137 4154 5606 5692 5704 6050 Size: 1413 367 385 17 1452 86 12 346 Cuts at: 6050 1972 Size: 1977 Fragments arranged by size: 1977 1452 1413 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2084 2356 2478 3121 3196 3225 513 Size: 552 1019 272 122 643 75 29 3343 Fragments arranged by size: 3343 1019 643 552 272 122 75 29 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5316 Fragments arranged by size: 5316 683 56 BtsI GCAGTG_nn' Cuts at: 3571 4365 4385 3571 Size: 794 20 5241 Fragments arranged by size: 5241 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6055 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6020 DraI TTT'AAA Cuts at: 3359 3359 Size: 6055 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5062 809 Size: 507 3746 1802 Fragments arranged by size: 3746 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2220 2317 2367 Size: 54 1425 50 275 18 390 97 50 Cuts at: 2367 2487 2874 3502 4391 6020 8 Size: 120 387 628 889 1629 43 Fragments arranged by size: 1629 1425 889 628 390 387 275 120 97 54 50 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2220 2317 2367 2874 3502 1537 Size: 275 408 97 50 507 628 4090 Fragments arranged by size: 4090 628 507 408 275 97 50 EarI CTCTTCn'nnn_ Cuts at: 1353 2034 2800 3985 1353 Size: 681 766 1185 3423 Fragments arranged by size: 3423 1185 766 681 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2095 2899 4642 5016 5162 387 Size: 1064 138 506 804 1743 374 146 1280 Fragments arranged by size: 1743 1280 1064 804 506 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2172 2417 2760 3315 3350 4118 4715 Size: 393 540 245 343 555 35 768 597 Cuts at: 4715 5502 5770 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 768 597 555 540 393 343 268 245 35 EcoO109I rG'GnC_Cy Cuts at: 2089 2591 2089 Size: 502 5553 EcoRI G'AATT_C Cuts at: 3086 3364 3742 3086 Size: 278 378 5399 Fragments arranged by size: 5399 378 278 FseI GG_CCGG'CC Cuts at: 2373 2373 Size: 6055 FspI TGC'GCA Cuts at: 4561 4561 Size: 6055 HaeII r_GCGC'y Cuts at: 1678 2288 2365 2418 5202 1678 Size: 610 77 53 2784 2531 Fragments arranged by size: 2784 2531 610 77 53 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2216 2248 2685 2717 4696 Size: 32 135 32 644 32 437 32 1979 Cuts at: 4696 4728 4770 4802 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1979 644 437 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2545 3399 3645 669 Size: 988 281 607 854 246 3079 Fragments arranged by size: 3079 988 854 607 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6055 HpaI GTT'AAC Cuts at: 3645 3645 Size: 6055 KasI G'GCGC_C Cuts at: 2284 2361 2284 Size: 77 5978 KpnI G_GTAC'C Cuts at: 3380 3380 Size: 6055 MfeI C'AATT_G Cuts at: 3654 3654 Size: 6055 MluI A'CGCG_T Cuts at: 5931 5931 Size: 6055 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2466 2829 5169 5353 1305 Size: 1161 363 2340 184 2007 Fragments arranged by size: 2340 2007 1161 363 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4233 Fragments arranged by size: 4233 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2600 2767 4013 4372 4531 518 Size: 648 1434 167 1246 359 159 2042 Fragments arranged by size: 2042 1434 1246 648 359 167 159 MspA1I CmG'CkG Cuts at: 1606 2086 2203 2480 3227 3311 3471 4148 5296 Size: 480 117 277 747 84 160 677 1148 Cuts at: 5296 5541 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 747 677 480 277 245 160 117 84 NaeI GCC'GGC Cuts at: 2283 2371 2283 Size: 88 5967 NarI GG'CG_CC Cuts at: 2285 2362 2285 Size: 77 5978 NcoI C'CATG_G Cuts at: 513 1065 3121 513 Size: 552 2056 3447 Fragments arranged by size: 3447 2056 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6055 NgoMIV G'CCGG_C Cuts at: 2281 2369 2281 Size: 88 5967 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6055 NotI GC'GGCC_GC Cuts at: 3502 3502 Size: 6055 NspI r_CATG'y Cuts at: 1132 1229 2515 3058 3413 4958 1132 Size: 97 1286 543 355 1545 2229 Fragments arranged by size: 2229 1545 1286 543 355 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6055 PciI A'CATG_T Cuts at: 4954 4954 Size: 6055 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2203 1108 Size: 902 193 4960 Fragments arranged by size: 4960 902 193 PmeI GTTT'AAAC Cuts at: 3359 3359 Size: 6055 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4124 4156 5637 5669 4124 Size: 32 1481 32 4510 Fragments arranged by size: 4510 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2089 2591 2089 Size: 502 5553 PshAI GACnn'nnGTC Cuts at: 1613 3303 1613 Size: 1690 4365 PsiI TTA'TAA Cuts at: 3625 3625 Size: 6055 PstI C_TGCA'G Cuts at: 830 3243 3407 830 Size: 2413 164 3478 Fragments arranged by size: 3478 2413 164 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6055 PvuII CAG'CTG Cuts at: 2203 3311 2203 Size: 1108 4947 SacII CC_GC'GG Cuts at: 2087 2481 3228 2087 Size: 394 747 4914 Fragments arranged by size: 4914 747 394 SalI G'TCGA_C Cuts at: 1936 2543 3397 1936 Size: 607 854 4594 Fragments arranged by size: 4594 854 607 SanDI GG'GwC_CC Cuts at: 2089 2089 Size: 6055 SapI GCTCTTCn'nnn_ Cuts at: 2800 2800 Size: 6055 SbfI CC_TGCA'GG Cuts at: 3407 3407 Size: 6055 ScaI AGT'ACT Cuts at: 4303 6015 4303 Size: 1712 4343 SfcI C'TryA_G Cuts at: 826 1045 2122 3239 3403 4538 5219 5410 826 Size: 219 1077 1117 164 1135 681 191 1471 Fragments arranged by size: 1471 1135 1117 1077 681 219 191 164 SfoI GGC'GCC Cuts at: 2286 2363 2286 Size: 77 5978 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6055 SmaI CCC'GGG Cuts at: 1872 3382 1872 Size: 1510 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4159 5060 5322 820 Size: 197 926 2216 901 262 1553 Fragments arranged by size: 2216 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3846 493 Size: 3353 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6055 SphI G_CATG'C Cuts at: 1132 3413 1132 Size: 2281 3774 SspI AAT'ATT Cuts at: 5 52 3002 3979 5 Size: 47 2950 977 2081 Fragments arranged by size: 2950 2081 977 47 StuI AGG'CCT Cuts at: 1517 2309 1517 Size: 792 5263 StyI C'CwwG_G Cuts at: 513 1065 3121 3494 513 Size: 552 2056 373 3074 Fragments arranged by size: 3074 2056 552 373 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4082 4099 4252 4437 4882 325 Size: 1255 2502 17 153 185 445 1498 Fragments arranged by size: 2502 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2743 4301 6013 Size: 274 80 33 51 1443 766 1558 1712 Cuts at: 6013 96 Size: 138 Fragments arranged by size: 1712 1558 1443 766 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2087 2119 6015 6047 2087 Size: 32 3896 32 2095 Fragments arranged by size: 3896 2095 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6055 XbaI T'CTAG_A Cuts at: 3391 3391 Size: 6055 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6055 XmaI C'CCGG_G Cuts at: 1870 3380 1870 Size: 1510 4545 XmnI GAAnn'nnTTC Cuts at: 2934 4184 2934 Size: 1250 4805 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3863 276 Size: 53 83 186 936 2329 2468 Fragments arranged by size: 2468 2329 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SexAI SfiI SrfI SwaI XcmI