################################## # NO CUT ENZYMES ( 175 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BmyI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrI BssHII Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI MwoI NaeI NarI NcoI NdeI NgoMIV NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AccI 148 GTCTAC GT^[AC][GT]AC AciI 269 CCGC CCGC(-3/-1) AfaI 813 GTAC GT^AC AlwI 1109 GGATC GGATC(4/5) AvaII 108 GGTCC G^G[AT]CC BcnI 1019 CCGGG CC^[GC]GG BglII 675 AGATCT A^GATCT BlpI 403 GCTGAGC GC^T[ACGT]AGC BpmI 550 CTGGAG CTGGAG(16/14) Bpu1102I 403 GCTGAGC GC^T[ACGT]AGC BsgI 238 GTGCAG GTGCAG(16/14) BsiYI 104 CCAAGGTCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 104 CCAAGGTCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 308 GAGAC GTCTC(1/5) Bsp1407I 812 TGTACA T^GTACA BsrGI 812 TGTACA T^GTACA BssSI 325 CTCGTG CACGAG(-5/-1) CelII 403 GCTGAGC GC^T[ACGT]AGC EaeI 112 CGGCCA [CT]^GGCC[GA] EarI 545 CTCTTC CTCTTC(1/4) Ksp632I 545 CTCTTC CTCTTC(1/4) MaeII 152 ACGT A^CGT NciI 1019 CCGGG CC^[GC]GG NheI 211 GCTAGC G^CTAGC NspI 603 GCATGT [GA]CATG^[CT] PshAI 1048 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 472 CTGCAG CTGCA^G PvuII 893 CAGCTG CAG^CTG RsaI 813 GTAC GT^AC SfaNI 878 GCATC GCATC(5/9) SspBI 812 TGTACA T^GTACA SspI 691 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AlwNI 284 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG 716 CAGCCTCTG BbsI 392 GAAGAC GAAGAC(2/6) 503 GTCTTC BpuAI 392 GAAGAC GAAGAC(2/6) 503 GTCTTC BstNI 388 CCAGG CC^[AT]GG 570 CCTGG BstYI 675 AGATCT [GA]^GATC[CT] 1109 GGATCT Cac8I 211 GCTAGC GC[ACGT]^[ACGT]GC 971 GCCAGC DdeI 34 CTTAG C^T[ACGT]AG 404 CTGAG DpnII 676 GATC ^GATC 1110 GATC EcoRII 388 CCAGG ^CC[AT]GG 570 CCTGG EcoT14I 104 CCAAGG C^C[AT][AT]GG 206 CCAAGG HapII 111 CCGG C^CGG 1019 CCGG HpaII 111 CCGG C^CGG 1019 CCGG MboI 676 GATC ^GATC 1110 GATC MflI 675 AGATCT [GA]^GATC[CT] 1109 GGATCT MslI 249 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 810 CATGTACATG MspA1I 269 CCGCTG C[AC]G^C[GT]G 893 CAGCTG MspI 111 CCGG C^CGG 1019 CCGG MvaI 388 CCAGG CC^[AT]GG 570 CCTGG NdeII 676 GATC ^GATC 1110 GATC Sau3AI 676 GATC ^GATC 1110 GATC StyI 104 CCAAGG C^C[AT][AT]GG 206 CCAAGG TaqI 582 TCGA T^CGA 654 TCGA TthHB8I 582 TCGA T^CGA 654 TCGA XhoII 675 AGATCT [GA]^GATC[CT] 1109 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AluI BbvI BfaI BsaJI BssKI Cfr13I Fnu4HI HaeIII HinfI HphI ItaI MaeI MaeIII MboII MnlI MseI NlaIII PleI Sau96I ScrFI SfcI TfiI Tru9I TseI Tsp45I Tsp509I TspRI