(Circular) (Six-base) MAPSORT of: pFN21AE3155.seq Check: 6785 from: 1 to: 5824 pFN21AE3155 5824 bp With 182 enzymes: SgfI * July 6, 2012 17:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3634 278 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2154 3167 1937 Size: 217 1013 4594 Fragments arranged by size: 4594 1013 217 Acc65I G'GTAC_C Cuts at: 3145 3145 Size: 5824 AclI AA'CG_TT Cuts at: 1563 3951 4324 1563 Size: 2388 373 3063 Fragments arranged by size: 3063 2388 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3887 5271 5539 1632 Size: 2255 1384 268 1917 Fragments arranged by size: 2255 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5627 AflIII A'CryG_T Cuts at: 1203 4723 5700 1203 Size: 3520 977 1327 Fragments arranged by size: 3520 1327 977 AgeI A'CCGG_T Cuts at: 4655 4655 Size: 5824 AhdI GACnn_n'nnGTC Cuts at: 4553 4553 Size: 5824 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5824 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2989 1184 Size: 1805 4019 AlwNI CAG_nnn'CTG Cuts at: 2294 2726 3567 5139 2294 Size: 432 841 1572 2979 Fragments arranged by size: 2979 1572 841 432 ApaLI G'TGCA_C Cuts at: 3881 5037 3881 Size: 1156 4668 ApoI r'AATT_y Cuts at: 1493 3133 3359 3511 5620 1493 Size: 1640 226 152 2109 1697 Fragments arranged by size: 2109 1697 1640 226 152 AseI AT'TA_AT Cuts at: 160 1032 4378 160 Size: 872 3346 1606 Fragments arranged by size: 3346 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 AvaI C'yCGr_G Cuts at: 1870 1943 3149 1870 Size: 73 1206 4545 Fragments arranged by size: 4545 1206 73 BamHI G'GATC_C Cuts at: 3154 3521 3154 Size: 367 5457 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3145 4600 618 Size: 325 217 627 1358 1455 1842 Fragments arranged by size: 1842 1455 1358 627 325 217 BanII G_rGCy'C Cuts at: 1391 3143 1391 Size: 1752 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2404 2501 928 Size: 971 505 97 4251 Fragments arranged by size: 4251 971 505 97 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4015 4049 1903 Size: 34 2078 34 3678 Fragments arranged by size: 3678 2078 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3716 4926 3716 Size: 1210 4614 BclI T'GATC_A Cuts at: 1548 1548 Size: 5824 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4435 136 Size: 107 122 71 631 605 2763 1525 Fragments arranged by size: 2763 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2680 5819 2680 Size: 3139 2685 BlpI GC'TnA_GC Cuts at: 2409 3241 2409 Size: 832 4992 Bme1580I G_kGCm'C Cuts at: 1790 3885 5041 1790 Size: 2095 1156 2573 Fragments arranged by size: 2573 2095 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5824 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3555 4513 442 Size: 416 53 83 399 24 2138 958 1753 Fragments arranged by size: 2138 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2220 1056 Size: 1164 4660 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2576 4484 1239 Size: 1337 1908 2579 Fragments arranged by size: 2579 1908 1337 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3949 4814 5112 3949 Size: 865 298 4661 Fragments arranged by size: 4661 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4487 882 Size: 617 2988 2219 Fragments arranged by size: 2988 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3615 493 Size: 711 2411 2702 Fragments arranged by size: 2702 2411 711 BsaBI GATnn'nnATC Cuts at: 5818 5818 Size: 5824 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3631 4013 4648 275 Size: 53 83 186 936 2098 382 635 1451 Fragments arranged by size: 2098 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4257 4655 4929 5076 5487 4257 Size: 398 274 147 411 4594 Fragments arranged by size: 4594 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2075 2105 2847 2877 1178 Size: 30 165 30 672 30 742 30 4125 Fragments arranged by size: 4125 742 672 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5824 BseYI C'CCAG_C Cuts at: 2427 5027 5523 2427 Size: 2600 496 2728 Fragments arranged by size: 2728 2600 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2264 1163 Size: 573 528 4723 Fragments arranged by size: 4723 573 528 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3274 4035 5063 1540 Size: 275 186 1273 761 1028 2301 Fragments arranged by size: 2301 1273 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3885 3970 5041 3885 Size: 85 1071 4668 Fragments arranged by size: 4668 1071 85 BsmI GAATG_Cn' Cuts at: 3335 3428 3335 Size: 93 5731 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3143 3885 3970 5041 1391 Size: 399 1353 742 85 1071 2174 Fragments arranged by size: 2174 1353 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5487 5487 Size: 5824 BspHI T'CATG_A Cuts at: 3713 5443 3713 Size: 1730 4094 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3179 844 Size: 2335 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3711 1394 Size: 429 1888 3507 Fragments arranged by size: 3507 1888 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4319 4493 65 Size: 1183 3071 174 1396 Fragments arranged by size: 3071 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4468 4655 1700 Size: 110 2658 187 2869 Fragments arranged by size: 2869 2658 187 110 BsrGI T'GTAC_A Cuts at: 96 2817 96 Size: 2721 3103 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5824 BssSI C'ACGA_G Cuts at: 2330 3884 4896 2330 Size: 1554 1012 3258 Fragments arranged by size: 3258 1554 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5824 BstBI TT'CG_AA Cuts at: 4628 4628 Size: 5824 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5824 BstYI r'GATC_y Cuts at: 1972 2680 3114 3154 3521 3906 3923 5375 5461 Size: 708 434 40 367 385 17 1452 86 Cuts at: 5461 5473 5819 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 708 434 385 367 346 86 40 17 12 BtgI C'CryG_G Cuts at: 513 1065 3120 513 Size: 552 2055 3217 Fragments arranged by size: 3217 2055 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2389 487 Size: 683 56 1163 3922 Fragments arranged by size: 3922 1163 683 56 BtsI GCAGTG_nn' Cuts at: 2474 3340 4134 4154 2474 Size: 866 794 20 4144 Fragments arranged by size: 4144 866 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5824 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5789 DraI TTT'AAA Cuts at: 3128 3128 Size: 5824 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4831 809 Size: 507 3515 1802 Fragments arranged by size: 3515 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2117 3271 4160 Size: 54 1425 50 275 18 287 1154 889 Cuts at: 4160 5789 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1154 889 287 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3271 1537 Size: 275 1459 4090 Fragments arranged by size: 4090 1459 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2556 3754 1353 Size: 1203 1198 3423 Fragments arranged by size: 3423 1203 1198 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4411 4785 4931 387 Size: 1064 138 2822 374 146 1280 Fragments arranged by size: 2822 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2576 3887 4484 5271 5539 1239 Size: 393 944 1311 597 787 268 1524 Fragments arranged by size: 1524 1311 944 787 597 393 268 EcoRI G'AATT_C Cuts at: 3133 3511 3133 Size: 378 5446 FspI TGC'GCA Cuts at: 4330 4330 Size: 5824 HaeII r_GCGC'y Cuts at: 1678 4971 1678 Size: 3293 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2333 2365 2473 2505 2652 Size: 32 135 32 761 32 108 32 147 Cuts at: 2652 2684 2824 2847 2856 2879 4465 4497 4539 Size: 32 140 23 9 23 1586 32 42 Cuts at: 4539 4571 1373 Size: 32 2626 Fragments arranged by size: 2626 1586 761 147 140 135 108 42 32 32 32 32 32 32 32 23 23 9 HincII GTy'rAC Cuts at: 669 1657 1938 3168 3414 669 Size: 988 281 1230 246 3079 Fragments arranged by size: 3079 1230 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5824 HpaI GTT'AAC Cuts at: 3414 3414 Size: 5824 KpnI G_GTAC'C Cuts at: 3149 3149 Size: 5824 MfeI C'AATT_G Cuts at: 3423 3423 Size: 5824 MluI A'CGCG_T Cuts at: 5700 5700 Size: 5824 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2309 4938 5122 1305 Size: 1004 2629 184 2007 Fragments arranged by size: 2629 2007 1004 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4002 Fragments arranged by size: 4002 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2258 2819 3782 4141 4300 518 Size: 648 1092 561 963 359 159 2042 Fragments arranged by size: 2042 1092 963 648 561 359 159 MspA1I CmG'CkG Cuts at: 1606 2276 2900 3240 3917 5065 5310 1606 Size: 670 624 340 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 670 624 340 245 NcoI C'CATG_G Cuts at: 513 1065 3120 513 Size: 552 2055 3217 Fragments arranged by size: 3217 2055 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5824 NheI G'CTAG_C Cuts at: 1052 2216 1052 Size: 1164 4660 NotI GC'GGCC_GC Cuts at: 3271 3271 Size: 5824 NspI r_CATG'y Cuts at: 1132 1229 2612 3182 4727 1132 Size: 97 1383 570 1545 2229 Fragments arranged by size: 2229 1545 1383 570 97 PasI CC'CwG_GG Cuts at: 1348 2575 1348 Size: 1227 4597 PciI A'CATG_T Cuts at: 4723 4723 Size: 5824 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5824 PmeI GTTT'AAAC Cuts at: 3128 3128 Size: 5824 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3893 3925 5406 5438 3893 Size: 32 1481 32 4279 Fragments arranged by size: 4279 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3057 1613 Size: 1444 4380 PsiI TTA'TAA Cuts at: 3394 3394 Size: 5824 PstI C_TGCA'G Cuts at: 830 2481 3176 830 Size: 1651 695 3478 Fragments arranged by size: 3478 1651 695 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5824 PvuII CAG'CTG Cuts at: 2900 2900 Size: 5824 SalI G'TCGA_C Cuts at: 1936 3166 1936 Size: 1230 4594 SbfI CC_TGCA'GG Cuts at: 3176 3176 Size: 5824 ScaI AGT'ACT Cuts at: 4072 5784 4072 Size: 1712 4112 SfcI C'TryA_G Cuts at: 826 1045 2337 2477 2901 3069 3172 4307 4988 Size: 219 1292 140 424 168 103 1135 681 Cuts at: 4988 5179 826 Size: 191 1471 Fragments arranged by size: 1471 1292 1135 681 424 219 191 168 140 103 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5824 SmaI CCC'GGG Cuts at: 1872 3151 1872 Size: 1279 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3928 4829 5091 820 Size: 197 926 1985 901 262 1553 Fragments arranged by size: 1985 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3615 493 Size: 3122 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5824 SphI G_CATG'C Cuts at: 1132 3182 1132 Size: 2050 3774 SspI AAT'ATT Cuts at: 5 52 2698 3748 5 Size: 47 2646 1050 2081 Fragments arranged by size: 2646 2081 1050 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5824 StyI C'CwwG_G Cuts at: 513 1065 2109 2211 3120 3263 513 Size: 552 1044 102 909 143 3074 Fragments arranged by size: 3074 1044 909 552 143 102 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2508 3851 3868 4021 4206 4651 325 Size: 1255 928 1343 17 153 185 445 1498 Fragments arranged by size: 1498 1343 1255 928 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2817 4070 5782 Size: 274 80 33 51 1443 840 1253 1712 Cuts at: 5782 96 Size: 138 Fragments arranged by size: 1712 1443 1253 840 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5784 5816 5784 Size: 32 5792 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5824 XbaI T'CTAG_A Cuts at: 3160 3160 Size: 5824 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5824 XmaI C'CCGG_G Cuts at: 1870 3149 1870 Size: 1279 4545 XmnI GAAnn'nnTTC Cuts at: 3953 3953 Size: 5824 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3632 276 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI