Restriction map for
pFN21AE1052
(Linear) (Six-base) MAPSORT of: KIEE1052.seq Check: 3484 from: 1 to: 1125
KIEE1052.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 0 551 1125
Size: 551 574
AfeI AGC'GCT
Cuts at: 0 44 370 1125
Size: 44 326 755
Fragments arranged by size:
755 326 44
AleI CACnn'nnGTG
Cuts at: 0 722 1125
Size: 722 403
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 895 927 1125
Size: 895 32 198
Fragments arranged by size:
895 198 32
AlwNI CAG_nnn'CTG
Cuts at: 0 428 1125
Size: 428 697
ApaLI G'TGCA_C
Cuts at: 0 34 1125
Size: 34 1091
ApoI r'AATT_y
Cuts at: 0 143 418 628 1125
Size: 143 275 210 497
Fragments arranged by size:
497 275 210 143
BanII G_rGCy'C
Cuts at: 0 484 896 1125
Size: 484 412 229
Fragments arranged by size:
484 412 229
BbsI GAAGACnn'nnnn_
Cuts at: 0 16 1040 1125
Size: 16 1024 85
Fragments arranged by size:
1024 85 16
BbvCI CC'TCA_GC
Cuts at: 0 349 1125
Size: 349 776
BglII A'GATC_T
Cuts at: 0 757 764 1125
Size: 757 7 361
Fragments arranged by size:
757 361 7
Bme1580I G_kGCm'C
Cuts at: 0 38 1125
Size: 38 1087
BmrI ACTGGGnnnn_n'
Cuts at: 0 797 1000 1125
Size: 797 203 125
Fragments arranged by size:
797 203 125
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 1125
Size: 67 1058
Bpu10I CC'TnA_GC
Cuts at: 0 349 1125
Size: 349 776
BsaAI yAC'GTr
Cuts at: 0 792 1125
Size: 792 333
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 895 925 1125
Size: 895 30 200
Fragments arranged by size:
895 200 30
BseYI C'CCAG_C
Cuts at: 0 428 532 633 648 1125
Size: 428 104 101 15 477
Fragments arranged by size:
477 428 104 101 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 861 1125
Size: 454 407 264
Fragments arranged by size:
454 407 264
BsiHKAI G_wGCw'C
Cuts at: 0 38 719 875 1125
Size: 38 681 156 250
Fragments arranged by size:
681 250 156 38
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1036 1125
Size: 1036 89
Bsp1286I G_dGCh'C
Cuts at: 0 38 484 719 875 896 1125
Size: 38 446 235 156 21 229
Fragments arranged by size:
446 235 229 156 38 21
BspHI T'CATG_A
Cuts at: 0 200 1125
Size: 200 925
BssSI C'ACGA_G
Cuts at: 0 1002 1125
Size: 1002 123
BstEII G'GTnAC_C
Cuts at: 0 591 1125
Size: 591 534
BstYI r'GATC_y
Cuts at: 0 644 757 764 920 1125
Size: 644 113 7 156 205
Fragments arranged by size:
644 205 156 113 7
BtsI GCAGTG_nn'
Cuts at: 0 1070 1125
Size: 1070 55
DrdI GACnn_nn'nnGTC
Cuts at: 0 600 1125
Size: 600 525
EaeI y'GGCC_r
Cuts at: 0 60 375 605 1125
Size: 60 315 230 520
Fragments arranged by size:
520 315 230 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 757 1125
Size: 757 368
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 1125
Size: 67 1058
EcoRI G'AATT_C
Cuts at: 0 418 628 1125
Size: 418 210 497
Fragments arranged by size:
497 418 210
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 705 1125
Size: 705 420
HaeII r_GCGC'y
Cuts at: 0 46 372 1125
Size: 46 326 753
Fragments arranged by size:
753 326 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 151 183 749 781 1125
Size: 151 32 566 32 344
Fragments arranged by size:
566 344 151 32 32
HincII GTy'rAC
Cuts at: 0 383 393 1125
Size: 383 10 732
Fragments arranged by size:
732 383 10
MscI TGG'CCA
Cuts at: 0 62 1125
Size: 62 1063
MslI CAynn'nnrTG
Cuts at: 0 722 868 1027 1125
Size: 722 146 159 98
Fragments arranged by size:
722 159 146 98
MspA1I CmG'CkG
Cuts at: 0 425 446 783 1125
Size: 425 21 337 342
Fragments arranged by size:
425 342 337 21
PasI CC'CwG_GG
Cuts at: 0 928 1125
Size: 928 197
PflMI CCAn_nnn'nTGG
Cuts at: 0 655 726 1125
Size: 655 71 399
Fragments arranged by size:
655 399 71
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 895 927 1125
Size: 895 32 198
Fragments arranged by size:
895 198 32
PshAI GACnn'nnGTC
Cuts at: 0 388 1125
Size: 388 737
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 506 538 1125
Size: 506 32 587
Fragments arranged by size:
587 506 32
PstI C_TGCA'G
Cuts at: 0 445 460 739 1125
Size: 445 15 279 386
Fragments arranged by size:
445 386 279 15
PvuII CAG'CTG
Cuts at: 0 425 783 1125
Size: 425 358 342
Fragments arranged by size:
425 358 342
SfcI C'TryA_G
Cuts at: 0 272 441 456 735 1125
Size: 272 169 15 279 390
Fragments arranged by size:
390 279 272 169 15
StuI AGG'CCT
Cuts at: 0 948 1125
Size: 948 177
StyI C'CwwG_G
Cuts at: 0 638 1125
Size: 638 487
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 293 420 1125
Size: 293 127 705
Fragments arranged by size:
705 293 127
Tth111I GACn'n_nGTC
Cuts at: 0 192 1125
Size: 192 933
Enzymes that do cut:
AccI AfeI AleI AloI AlwNI ApaLI ApoI BanII
BbsI BbvCI BglII Bme1580I BmrI BpmI Bpu10I BsaAI
BsaXI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BssSI
BstEII BstYI BtsI DrdI EaeI EciI Eco57MI EcoRI
FalI HaeII Hin4I HincII MscI MslI MspA1I PasI
PflMI PpiI PshAI PsrI PstI PvuII SfcI StuI
StyI TaqII Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlfI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BcgI BciVI BclI BfrBI
BglI BlpI BmgBI BmtI BplI BpuEI BsaI BsaBI
BsaHI BsaWI BseRI BsiEI BsiWI BsmI BspEI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstXI
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII
EagI EarI EcoICRI EcoNI EcoO109I EcoRV FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PfoI PmeI PmlI PpuMI
PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TatI TstI XbaI XcmI XhoI XmaI XmnI
ZraI