(Circular) (Six-base) MAPSORT of: pFN21AE1048.seq Check: 9466 from: 1 to: 5896 pFN21AE1048 5896 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3706 278 Size: 53 83 186 936 2170 2468 Fragments arranged by size: 2468 2170 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2661 3239 1937 Size: 724 578 4594 Fragments arranged by size: 4594 724 578 Acc65I G'GTAC_C Cuts at: 3217 3217 Size: 5896 AclI AA'CG_TT Cuts at: 1563 4023 4396 1563 Size: 2460 373 3063 Fragments arranged by size: 3063 2460 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2178 2358 2936 3959 5343 5611 1632 Size: 546 180 578 1023 1384 268 1917 Fragments arranged by size: 1917 1384 1023 578 546 268 180 AflII C'TTAA_G Cuts at: 820 1017 2936 820 Size: 197 1919 3780 Fragments arranged by size: 3780 1919 197 AflIII A'CryG_T Cuts at: 1203 4795 5772 1203 Size: 3592 977 1327 Fragments arranged by size: 3592 1327 977 AgeI A'CCGG_T Cuts at: 4727 4727 Size: 5896 AhdI GACnn_n'nnGTC Cuts at: 4625 4625 Size: 5896 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5896 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2198 1184 Size: 1014 4882 AlwNI CAG_nnn'CTG Cuts at: 3639 5211 3639 Size: 1572 4324 ApaLI G'TGCA_C Cuts at: 3953 5109 3953 Size: 1156 4740 ApoI r'AATT_y Cuts at: 1493 2105 2319 3205 3431 3583 5692 1493 Size: 612 214 886 226 152 2109 1697 Fragments arranged by size: 2109 1697 886 612 226 214 152 AseI AT'TA_AT Cuts at: 160 1032 2268 4450 160 Size: 872 1236 2182 1606 Fragments arranged by size: 2182 1606 1236 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5896 AvaI C'yCGr_G Cuts at: 1870 1943 3221 1870 Size: 73 1278 4545 Fragments arranged by size: 4545 1278 73 BamHI G'GATC_C Cuts at: 3226 3593 3226 Size: 367 5529 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2614 3217 4672 618 Size: 325 217 627 827 603 1455 1842 Fragments arranged by size: 1842 1455 827 627 603 325 217 BanII G_rGCy'C Cuts at: 1391 3215 1391 Size: 1824 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2636 928 Size: 971 737 4188 Fragments arranged by size: 4188 971 737 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4087 4121 1903 Size: 34 2150 34 3678 Fragments arranged by size: 3678 2150 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3788 4998 3788 Size: 1210 4686 BclI T'GATC_A Cuts at: 1548 1548 Size: 5896 BfrBI ATG'CAT Cuts at: 2753 2753 Size: 5896 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4507 136 Size: 107 122 71 631 605 2835 1525 Fragments arranged by size: 2835 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5891 5891 Size: 5896 BlpI GC'TnA_GC Cuts at: 2040 3313 2040 Size: 1273 4623 Bme1580I G_kGCm'C Cuts at: 1790 2914 3957 5113 1790 Size: 1124 1043 1156 2573 Fragments arranged by size: 2573 1156 1124 1043 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5896 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2974 3074 3627 Size: 416 53 83 399 24 1557 100 553 Cuts at: 3627 4585 442 Size: 958 1753 Fragments arranged by size: 1753 1557 958 553 416 399 100 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5896 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2394 2394 Size: 5896 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2697 2796 4556 1239 Size: 1458 99 1760 2579 Fragments arranged by size: 2579 1760 1458 99 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4021 4886 5184 4021 Size: 865 298 4733 Fragments arranged by size: 4733 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2243 4559 882 Size: 617 744 2316 2219 Fragments arranged by size: 2316 2219 744 617 BsaAI yAC'GTr Cuts at: 493 1204 3687 493 Size: 711 2483 2702 Fragments arranged by size: 2702 2483 711 BsaBI GATnn'nnATC Cuts at: 3076 5890 3076 Size: 2814 3082 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3115 3703 4085 4720 Size: 53 83 186 936 1582 588 382 635 Cuts at: 4720 275 Size: 1451 Fragments arranged by size: 1582 1451 936 635 588 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4329 4727 5001 5148 5559 4329 Size: 398 274 147 411 4666 Fragments arranged by size: 4666 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5671 Fragments arranged by size: 5671 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3013 3054 1186 Size: 1827 41 4028 Fragments arranged by size: 4028 1827 41 BseYI C'CCAG_C Cuts at: 2254 5099 5595 2254 Size: 2845 496 2555 Fragments arranged by size: 2845 2555 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3075 1163 Size: 573 1339 3984 Fragments arranged by size: 3984 1339 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3346 4107 5135 1540 Size: 275 186 1345 761 1028 2301 Fragments arranged by size: 2301 1345 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3191 3957 4042 5113 3191 Size: 766 85 1071 3974 Fragments arranged by size: 3974 1071 766 85 BsmI GAATG_Cn' Cuts at: 2755 3407 3500 2755 Size: 652 93 5151 Fragments arranged by size: 5151 652 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2914 3191 3215 3957 4042 5113 1391 Size: 399 1124 277 24 742 85 1071 2174 Fragments arranged by size: 2174 1124 1071 742 399 277 85 24 BspEI T'CCGG_A Cuts at: 5559 5559 Size: 5896 BspHI T'CATG_A Cuts at: 3785 5515 3785 Size: 1730 4166 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2647 3251 844 Size: 1803 604 3489 Fragments arranged by size: 3489 1803 604 BsrBI CCG'CTC Cuts at: 1394 1823 3783 1394 Size: 429 1960 3507 Fragments arranged by size: 3507 1960 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2182 2496 3045 4391 4565 65 Size: 1183 934 314 549 1346 174 1396 Fragments arranged by size: 1396 1346 1183 934 549 314 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4540 4727 1700 Size: 110 2730 187 2869 Fragments arranged by size: 2869 2730 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5896 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5896 BssSI C'ACGA_G Cuts at: 3956 4968 3956 Size: 1012 4884 BstAPI GCAn_nnn'nTGC Cuts at: 794 2495 2750 794 Size: 1701 255 3940 Fragments arranged by size: 3940 1701 255 BstBI TT'CG_AA Cuts at: 4700 4700 Size: 5896 BstEII G'GTnAC_C Cuts at: 1184 3056 1184 Size: 1872 4024 BstXI CCAn_nnnn'nTGG Cuts at: 3052 3052 Size: 5896 BstYI r'GATC_y Cuts at: 1972 3007 3071 3226 3593 3978 3995 5447 5533 Size: 1035 64 155 367 385 17 1452 86 Cuts at: 5533 5545 5891 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1035 385 367 346 155 86 64 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2484 513 Size: 552 939 480 3925 Fragments arranged by size: 3925 939 552 480 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5157 Fragments arranged by size: 5157 683 56 BtsI GCAGTG_nn' Cuts at: 2085 3412 4206 4226 2085 Size: 1327 794 20 3755 Fragments arranged by size: 3755 1327 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5896 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2823 2858 529 Size: 35 2259 35 3567 Fragments arranged by size: 3567 2259 35 35 DraI TTT'AAA Cuts at: 3200 3200 Size: 5896 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4903 809 Size: 507 3587 1802 Fragments arranged by size: 3587 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3160 3343 4232 Size: 54 1425 50 275 18 1330 183 889 Cuts at: 4232 5861 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1330 889 275 183 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3343 1537 Size: 275 1531 4090 Fragments arranged by size: 4090 1531 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3826 1353 Size: 2473 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4483 4857 5003 387 Size: 1064 138 2894 374 146 1280 Fragments arranged by size: 2894 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2178 2358 2697 2796 2936 3959 4556 Size: 393 546 180 339 99 140 1023 597 Cuts at: 4556 5343 5611 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1023 787 597 546 393 339 268 180 140 99 EcoRI G'AATT_C Cuts at: 3205 3583 3205 Size: 378 5518 EcoRV GAT'ATC Cuts at: 2428 2428 Size: 5896 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2140 2140 Size: 5896 FspI TGC'GCA Cuts at: 4402 4402 Size: 5896 HaeII r_GCGC'y Cuts at: 1678 5043 1678 Size: 3365 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2242 2274 2412 2444 2769 Size: 32 135 32 670 32 138 32 325 Cuts at: 2769 2801 2992 3024 4537 4569 4611 4643 1373 Size: 32 191 32 1513 32 42 32 2626 Fragments arranged by size: 2626 1513 670 325 191 138 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2158 3240 3486 669 Size: 988 281 220 1082 246 3079 Fragments arranged by size: 3079 1082 988 281 246 220 HindIII A'AGCT_T Cuts at: 748 2414 2967 748 Size: 1666 553 3677 Fragments arranged by size: 3677 1666 553 HpaI GTT'AAC Cuts at: 3486 3486 Size: 5896 KpnI G_GTAC'C Cuts at: 3221 3221 Size: 5896 MfeI C'AATT_G Cuts at: 2619 3495 2619 Size: 876 5020 MluI A'CGCG_T Cuts at: 5772 5772 Size: 5896 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3118 5010 5194 1305 Size: 1813 1892 184 2007 Fragments arranged by size: 2007 1892 1813 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3162 10 Size: 54 1425 343 1330 2744 Fragments arranged by size: 2744 1425 1330 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2489 3854 4213 4372 518 Size: 648 1323 1365 359 159 2042 Fragments arranged by size: 2042 1365 1323 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3312 3989 5137 5382 1606 Size: 1706 677 1148 245 2120 Fragments arranged by size: 2120 1706 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2484 513 Size: 552 939 480 3925 Fragments arranged by size: 3925 939 552 480 NdeI CA'TA_TG Cuts at: 387 387 Size: 5896 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5896 NotI GC'GGCC_GC Cuts at: 3343 3343 Size: 5896 NsiI A_TGCA'T Cuts at: 2755 2755 Size: 5896 NspI r_CATG'y Cuts at: 1132 1229 2222 2474 2672 3254 4799 1132 Size: 97 993 252 198 582 1545 2229 Fragments arranged by size: 2229 1545 993 582 252 198 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5896 PciI A'CATG_T Cuts at: 4795 4795 Size: 5896 PflMI CCAn_nnn'nTGG Cuts at: 1108 2986 1108 Size: 1878 4018 PmeI GTTT'AAAC Cuts at: 3200 3200 Size: 5896 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3965 3997 5478 5510 3965 Size: 32 1481 32 4351 Fragments arranged by size: 4351 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5896 PsiI TTA'TAA Cuts at: 3466 3466 Size: 5896 PstI C_TGCA'G Cuts at: 830 2187 2500 2559 2746 3248 830 Size: 1357 313 59 187 502 3478 Fragments arranged by size: 3478 1357 502 313 187 59 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5896 SalI G'TCGA_C Cuts at: 1936 3238 1936 Size: 1302 4594 SbfI CC_TGCA'GG Cuts at: 3248 3248 Size: 5896 ScaI AGT'ACT Cuts at: 2984 4144 5856 2984 Size: 1160 1712 3024 Fragments arranged by size: 3024 1712 1160 SexAI A'CCwGG_T Cuts at: 3096 3096 Size: 5896 SfcI C'TryA_G Cuts at: 826 1045 2183 2496 2555 2742 3244 4379 5060 Size: 219 1138 313 59 187 502 1135 681 Cuts at: 5060 5251 826 Size: 191 1471 Fragments arranged by size: 1471 1138 1135 681 502 313 219 191 187 59 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5896 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5896 SmaI CCC'GGG Cuts at: 1872 3223 1872 Size: 1351 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2936 4000 4901 5163 820 Size: 197 926 993 1064 901 262 1553 Fragments arranged by size: 1553 1064 993 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3687 493 Size: 3194 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5896 SphI G_CATG'C Cuts at: 1132 2672 3254 1132 Size: 1540 582 3774 Fragments arranged by size: 3774 1540 582 SspI AAT'ATT Cuts at: 5 52 3820 5 Size: 47 3768 2081 Fragments arranged by size: 3768 2081 47 StuI AGG'CCT Cuts at: 1517 2070 1517 Size: 553 5343 StyI C'CwwG_G Cuts at: 513 1065 2004 2115 2484 3163 3335 513 Size: 552 939 111 369 679 172 3074 Fragments arranged by size: 3074 939 679 552 369 172 111 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3075 3923 3940 4093 4278 4723 325 Size: 1255 1495 848 17 153 185 445 1498 Fragments arranged by size: 1498 1495 1255 848 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2982 4142 5854 Size: 274 80 33 51 1443 1005 1160 1712 Cuts at: 5854 96 Size: 138 Fragments arranged by size: 1712 1443 1160 1005 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5856 5888 5856 Size: 32 5864 Tth111I GACn'n_nGTC Cuts at: 1316 2931 1316 Size: 1615 4281 XbaI T'CTAG_A Cuts at: 3232 3232 Size: 5896 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5896 XmaI C'CCGG_G Cuts at: 1870 3221 1870 Size: 1351 4545 XmnI GAAnn'nnTTC Cuts at: 2081 4025 2081 Size: 1944 3952 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3704 276 Size: 53 83 186 936 2170 2468 Fragments arranged by size: 2468 2170 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI