################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI Fnu4HI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI ItaI KasI KpnI Ksp632I KspI MaeI MaeIII MamI MfeI MluI MroI MseI MunI MvnI NarI NciI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I TseI Tsp45I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 12 GTCTAC GT^[AC][GT]AC AcsI 190 AAATTC [GA]^AATT[CT] AfaI 92 GTAC GT^AC AocI 481 CCTGAGG CC^T[ACGT]AGG ApoI 190 AAATTC [GA]^AATT[CT] AspHI 509 GTGCTC G[AT]GC[AT]^C AvaI 346 CTCGGG C^[CT]CG[GA]G BalI 77 TGGCCA TGG^CCA BbsI 327 GAAGAC GAAGAC(2/6) BglII 210 AGATCT A^GATCT BmyI 509 GTGCTC G[AGT]GC[ACT]^C BpuAI 327 GAAGAC GAAGAC(2/6) BsaI 352 GAGACC GGTCTC(1/5) BsgI 449 GTGCAG GTGCAG(16/14) BsiHKAI 509 GTGCTC G[AT]GC[AT]^C BsmFI 379 GGGAC GGGAC(10/14) BsoBI 346 CTCGGG C^[CT]CG[GA]G Bsp1286I 509 GTGCTC G[AGT]GC[ACT]^C BsrDI 99 CATTGC GCAATG(2/0) BssSI 501 CACGAG CACGAG(-5/-1) BstF5I 384 CATCC GGATG(2/0) Bsu36I 481 CCTGAGG CC^T[ACGT]AGG DraII 42 GGGGCCT [GA]G^G[ACGT]CC[CT] EaeI 77 TGGCCA [CT]^GGCC[GA] Eco81I 481 CCTGAGG CC^T[ACGT]AGG EcoO109I 42 GGGGCCT [GA]G^G[ACGT]CC[CT] FauI 126 GCGGG CCCGC(4/6) FokI 384 CATCC GGATG(9/13) HincII 232 GTTGAC GT[CT]^[GA]AC HindII 232 GTTGAC GT[CT]^[GA]AC HindIII 200 AAGCTT A^AGCTT MaeII 230 ACGT A^CGT MluNI 77 TGGCCA TGG^CCA MscI 77 TGGCCA TGG^CCA MslI 427 CATGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 124 CAGCGG C[AC]G^C[GT]G NaeI 259 GCCGGC GCC^GGC NgoMIV 259 GCCGGC G^CCGGC NspI 275 ACATGC [GA]CATG^[CT] PflMI 299 CCATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 362 CTGCAG CTGCA^G RsaI 92 GTAC GT^AC SfcI 362 CTGCAG C^T[GA][CT]AG Tsp509I 191 AATT ^AATT TspRI 432 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 299 CCATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AciI 174 CCGC CCGC(-3/-1) 126 GCGG AlwI 23 GGATC GGATC(4/5) 521 GGATC Asp700I 165 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 208 GAAGATCTTC BsiYI 299 CCATGGTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 371 CCAAGCCTGGG BslI 299 CCATGGTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 371 CCAAGCCTGGG BsmAI 352 GAGAC GTCTC(1/5) 392 GAGAC BsrFI 259 GCCGGC [GA]^CCGG[CT] 288 GCCGGT BsrI 489 ACTGG ACTGG(1/-1) 518 ACTGG BssKI 264 CCTGG ^CC[ACGT]GG 376 CCTGG BstNI 264 CCTGG CC^[AT]GG 376 CCTGG BstYI 210 AGATCT [GA]^GATC[CT] 521 GGATCT Cac8I 198 GCAAGC GC[ACGT]^[ACGT]GC 259 GCCGGC Cfr10I 259 GCCGGC [GA]^CCGG[CT] 288 GCCGGT EcoRII 264 CCTGG ^CC[AT]GG 376 CCTGG EcoT14I 74 CCATGG C^C[AT][AT]GG 299 CCATGG HapII 260 CCGG C^CGG 289 CCGG HpaII 260 CCGG C^CGG 289 CCGG MflI 210 AGATCT [GA]^GATC[CT] 521 GGATCT MspI 260 CCGG C^CGG 289 CCGG MvaI 264 CCTGG CC^[AT]GG 376 CCTGG MwoI 153 GCGTTTTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 279 GCACTCAGGGC NcoI 74 CCATGG C^CATGG 299 CCATGG PleI 367 GACTC GAGTC(4/5) 338 GAGTC ScrFI 264 CCTGG CC^[ACGT]GG 376 CCTGG SmlI 54 CTCAAG C^T[CT][GA]AG 217 CTCAAG StyI 74 CCATGG C^C[AT][AT]GG 299 CCATGG TfiI 170 GATTC G^A[AT]TC 436 GATTC XhoII 210 AGATCT [GA]^GATC[CT] 521 GGATCT XmnI 165 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 208 GAAGATCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AluI AvaII BsaJI Cfr13I DdeI DpnII DsaI HaeIII HinfI HphI MboI MboII MnlI NdeII NlaIII NlaIV Sau3AI Sau96I