(Circular) (Six-base) MAPSORT of: pFN21AE3209.seq Check: 813 from: 1 to: 6139 pFN21AE3209 6139 bp With 182 enzymes: SgfI * June 13, 2018 20:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2526 3052 2526 Size: 526 5613 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3949 278 Size: 53 83 186 936 2413 2468 Fragments arranged by size: 2468 2413 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2331 3482 1937 Size: 394 1151 4594 Fragments arranged by size: 4594 1151 394 Acc65I G'GTAC_C Cuts at: 3141 3460 3141 Size: 319 5820 AclI AA'CG_TT Cuts at: 1563 4266 4639 1563 Size: 2703 373 3063 Fragments arranged by size: 3063 2703 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2293 2460 3167 3416 4202 5586 5854 1632 Size: 661 167 707 249 786 1384 268 1917 Fragments arranged by size: 1917 1384 786 707 661 268 249 167 AfeI AGC'GCT Cuts at: 2439 2439 Size: 6139 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5942 AflIII A'CryG_T Cuts at: 1203 3411 5038 6015 1203 Size: 2208 1627 977 1327 Fragments arranged by size: 2208 1627 1327 977 AgeI A'CCGG_T Cuts at: 4970 4970 Size: 6139 AhdI GACnn_n'nnGTC Cuts at: 4868 4868 Size: 6139 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6139 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6139 AlwNI CAG_nnn'CTG Cuts at: 2053 2518 3067 3882 5454 2053 Size: 465 549 815 1572 2738 Fragments arranged by size: 2738 1572 815 549 465 ApaI G_GGCC'C Cuts at: 2046 2116 2210 2501 2895 2046 Size: 70 94 291 394 5290 Fragments arranged by size: 5290 394 291 94 70 ApaLI G'TGCA_C Cuts at: 4196 5352 4196 Size: 1156 4983 ApoI r'AATT_y Cuts at: 1493 3448 3674 3826 5935 1493 Size: 1955 226 152 2109 1697 Fragments arranged by size: 2109 1955 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4693 160 Size: 872 3661 1606 Fragments arranged by size: 3661 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6139 AvaI C'yCGr_G Cuts at: 1870 1943 2032 2039 2433 2441 2698 2825 3464 Size: 73 89 7 394 8 257 127 639 Cuts at: 3464 1870 Size: 4545 Fragments arranged by size: 4545 639 394 257 127 89 73 8 7 BamHI G'GATC_C Cuts at: 3469 3836 3469 Size: 367 5772 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2178 2584 2704 3141 3460 Size: 325 217 627 391 406 120 437 319 Cuts at: 3460 4915 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 437 406 391 325 319 217 120 BanII G_rGCy'C Cuts at: 1391 2046 2116 2210 2501 2772 2832 2895 3327 Size: 655 70 94 291 271 60 63 432 Cuts at: 3327 3458 1391 Size: 131 4072 Fragments arranged by size: 4072 655 432 291 271 131 94 70 63 60 BbeI G_GCGC'C Cuts at: 2588 2588 Size: 6139 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3089 928 Size: 971 1190 3978 Fragments arranged by size: 3978 1190 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4330 4364 1903 Size: 34 2393 34 3678 Fragments arranged by size: 3678 2393 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4031 5241 4031 Size: 1210 4929 BclI T'GATC_A Cuts at: 1548 1548 Size: 6139 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2271 2428 2652 Size: 107 122 71 631 605 599 157 224 Cuts at: 2652 2882 4750 136 Size: 230 1868 1525 Fragments arranged by size: 1868 1525 631 605 599 230 224 157 122 107 71 BglII A'GATC_T Cuts at: 6134 6134 Size: 6139 BlpI GC'TnA_GC Cuts at: 3556 3556 Size: 6139 Bme1580I G_kGCm'C Cuts at: 1790 2046 2116 2210 2501 2709 2895 4200 5356 Size: 256 70 94 291 208 186 1305 1156 Cuts at: 5356 1790 Size: 2573 Fragments arranged by size: 2573 1305 1156 291 256 208 186 94 70 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6139 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2898 3870 4828 Size: 416 53 83 399 24 1481 972 958 Cuts at: 4828 442 Size: 1753 Fragments arranged by size: 1753 1481 972 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6139 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2882 4799 1239 Size: 917 726 1917 2579 Fragments arranged by size: 2579 1917 917 726 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4264 5129 5427 4264 Size: 865 298 4976 Fragments arranged by size: 4976 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2814 4802 882 Size: 617 1315 1988 2219 Fragments arranged by size: 2219 1988 1315 617 BsaAI yAC'GTr Cuts at: 493 1204 3930 493 Size: 711 2726 2702 Fragments arranged by size: 2726 2702 711 BsaBI GATnn'nnATC Cuts at: 3378 6133 3378 Size: 2755 3384 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2585 3946 4328 4963 Size: 53 83 186 936 1052 1361 382 635 Cuts at: 4963 275 Size: 1451 Fragments arranged by size: 1451 1361 1052 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4572 4970 5244 5391 5802 4572 Size: 398 274 147 411 4909 Fragments arranged by size: 4909 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5914 Fragments arranged by size: 5914 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2654 2702 1186 Size: 1468 48 4623 Fragments arranged by size: 4623 1468 48 BseYI C'CCAG_C Cuts at: 2046 2210 2673 2714 2978 5342 5838 2046 Size: 164 463 41 264 2364 496 2347 Fragments arranged by size: 2364 2347 496 463 264 164 41 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5566 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2142 2196 2282 3589 4350 5378 Size: 275 186 141 54 86 1307 761 1028 Cuts at: 5378 1540 Size: 2301 Fragments arranged by size: 2301 1307 1028 761 275 186 141 86 54 BsiHKAI G_wGCw'C Cuts at: 3134 4200 4285 5356 3134 Size: 1066 85 1071 3917 Fragments arranged by size: 3917 1071 1066 85 BsmI GAATG_Cn' Cuts at: 3650 3743 3650 Size: 93 6046 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2116 2210 2501 2709 2772 2832 Size: 399 256 70 94 291 208 63 60 Cuts at: 2832 2895 3134 3327 3458 4200 4285 5356 1391 Size: 63 239 193 131 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 291 256 239 208 193 131 94 85 70 63 63 60 BspEI T'CCGG_A Cuts at: 5802 5802 Size: 6139 BspHI T'CATG_A Cuts at: 4028 5758 4028 Size: 1730 4409 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2526 3052 3494 844 Size: 1682 526 442 3489 Fragments arranged by size: 3489 1682 526 442 BsrBI CCG'CTC Cuts at: 1394 1823 2021 4026 1394 Size: 429 198 2005 3507 Fragments arranged by size: 3507 2005 429 198 BsrDI GCAATG_nn' Cuts at: 65 1248 4634 4808 65 Size: 1183 3386 174 1396 Fragments arranged by size: 3386 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2099 2265 4783 4970 1700 Size: 110 289 166 2518 187 2869 Fragments arranged by size: 2869 2518 289 187 166 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6139 BssHII G'CGCG_C Cuts at: 1925 2274 2938 1925 Size: 349 664 5126 Fragments arranged by size: 5126 664 349 BssSI C'ACGA_G Cuts at: 4199 5211 4199 Size: 1012 5127 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6139 BstBI TT'CG_AA Cuts at: 4943 4943 Size: 6139 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6139 BstXI CCAn_nnnn'nTGG Cuts at: 2581 2755 2779 2581 Size: 174 24 5941 Fragments arranged by size: 5941 174 24 BstYI r'GATC_y Cuts at: 1972 2167 3469 3836 4221 4238 5690 5776 5788 Size: 195 1302 367 385 17 1452 86 12 Cuts at: 5788 6134 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1302 385 367 346 195 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2136 2459 2566 2574 3417 513 Size: 552 939 132 323 107 8 843 3235 Fragments arranged by size: 3235 939 843 552 323 132 107 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5400 Fragments arranged by size: 5400 683 56 BtsI GCAGTG_nn' Cuts at: 2129 2790 3116 3655 4449 4469 2129 Size: 661 326 539 794 20 3799 Fragments arranged by size: 3799 794 661 539 326 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6139 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6104 DraI TTT'AAA Cuts at: 3443 3443 Size: 6139 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5146 809 Size: 507 3830 1802 Fragments arranged by size: 3830 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2097 2139 2193 Size: 54 1425 50 275 18 267 42 54 Cuts at: 2193 2279 2394 2547 2757 3005 3051 3586 4475 Size: 86 115 153 210 248 46 535 889 Cuts at: 4475 6104 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 535 275 267 248 210 153 115 86 54 54 50 46 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 2139 2193 2279 3586 1537 Size: 275 327 54 86 1307 4090 Fragments arranged by size: 4090 1307 327 275 86 54 EarI CTCTTCn'nnn_ Cuts at: 1353 2528 3371 4069 1353 Size: 1175 843 698 3423 Fragments arranged by size: 3423 1175 843 698 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2659 4726 5100 5246 387 Size: 1064 138 1070 2067 374 146 1280 Fragments arranged by size: 2067 1280 1070 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2293 2460 2882 3167 3416 4202 Size: 393 524 137 167 422 285 249 786 Cuts at: 4202 4799 5586 5854 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 786 597 524 422 393 285 268 249 167 137 EcoNI CCTnn'n_nnAGG Cuts at: 2606 2606 Size: 6139 EcoO109I rG'GnC_Cy Cuts at: 2036 2043 2112 2113 2207 2695 2892 3361 2036 Size: 7 69 1 94 488 197 469 4814 Fragments arranged by size: 4814 488 469 197 94 69 7 1 EcoRI G'AATT_C Cuts at: 3448 3826 3448 Size: 378 5761 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3172 3172 Size: 6139 FspI TGC'GCA Cuts at: 4645 4645 Size: 6139 HaeII r_GCGC'y Cuts at: 1678 2217 2441 2588 2996 5286 1678 Size: 539 224 147 408 2290 2531 Fragments arranged by size: 2531 2290 539 408 224 147 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3241 3273 4780 4812 4854 Size: 32 135 32 1669 32 1507 32 42 Cuts at: 4854 4886 1373 Size: 32 2626 Fragments arranged by size: 2626 1669 1507 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2332 3483 3729 669 Size: 988 281 394 1151 246 3079 Fragments arranged by size: 3079 1151 988 394 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6139 HpaI GTT'AAC Cuts at: 3729 3729 Size: 6139 KasI G'GCGC_C Cuts at: 2584 2584 Size: 6139 KpnI G_GTAC'C Cuts at: 3145 3464 3145 Size: 319 5820 MfeI C'AATT_G Cuts at: 3738 3738 Size: 6139 MluI A'CGCG_T Cuts at: 6015 6015 Size: 6139 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3288 5253 5437 1305 Size: 1983 1965 184 2007 Fragments arranged by size: 2007 1983 1965 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2759 10 Size: 54 1425 343 927 3390 Fragments arranged by size: 3390 1425 927 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3416 4097 4456 4615 518 Size: 648 2250 681 359 159 2042 Fragments arranged by size: 2250 2042 681 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2050 2138 2176 2461 2488 2568 2677 2855 Size: 444 88 38 285 27 80 109 178 Cuts at: 2855 2971 3555 4232 5380 5625 1606 Size: 116 584 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 584 444 285 245 178 116 109 88 80 38 27 NaeI GCC'GGC Cuts at: 2101 2267 2101 Size: 166 5973 NarI GG'CG_CC Cuts at: 2585 2585 Size: 6139 NcoI C'CATG_G Cuts at: 513 1065 2004 2574 513 Size: 552 939 570 4078 Fragments arranged by size: 4078 939 570 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6139 NgoMIV G'CCGG_C Cuts at: 2099 2265 2099 Size: 166 5973 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6139 NotI GC'GGCC_GC Cuts at: 2139 2193 3586 2139 Size: 54 1393 4692 Fragments arranged by size: 4692 1393 54 NspI r_CATG'y Cuts at: 1132 1229 2242 3231 3415 3497 5042 1132 Size: 97 1013 989 184 82 1545 2229 Fragments arranged by size: 2229 1545 1013 989 184 97 82 PasI CC'CwG_GG Cuts at: 1348 2183 2494 1348 Size: 835 311 4993 Fragments arranged by size: 4993 835 311 PciI A'CATG_T Cuts at: 3411 5038 3411 Size: 1627 4512 PflMI CCAn_nnn'nTGG Cuts at: 1108 2224 1108 Size: 1116 5023 PfoI T'CCnGG_A Cuts at: 2088 2898 3134 3369 2088 Size: 810 236 235 4858 Fragments arranged by size: 4858 810 236 235 PmeI GTTT'AAAC Cuts at: 3443 3443 Size: 6139 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4208 4240 5721 5753 4208 Size: 32 1481 32 4594 Fragments arranged by size: 4594 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2036 2695 3361 2036 Size: 659 666 4814 Fragments arranged by size: 4814 666 659 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6139 PsiI TTA'TAA Cuts at: 3709 3709 Size: 6139 PspOMI G'GGCC_C Cuts at: 2042 2112 2206 2497 2891 2042 Size: 70 94 291 394 5290 Fragments arranged by size: 5290 394 291 94 70 PspXI vC'TCGA_Gb Cuts at: 2433 2441 2433 Size: 8 6131 PstI C_TGCA'G Cuts at: 830 2157 2796 3491 830 Size: 1327 639 695 3478 Fragments arranged by size: 3478 1327 695 639 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6139 PvuII CAG'CTG Cuts at: 2050 2677 2971 2050 Size: 627 294 5218 Fragments arranged by size: 5218 627 294 RsrII CG'GwC_CG Cuts at: 2299 2299 Size: 6139 SacII CC_GC'GG Cuts at: 2139 2462 2569 2139 Size: 323 107 5709 Fragments arranged by size: 5709 323 107 SalI G'TCGA_C Cuts at: 1936 2330 3481 1936 Size: 394 1151 4594 Fragments arranged by size: 4594 1151 394 SanDI GG'GwC_CC Cuts at: 2036 2695 2036 Size: 659 5480 SapI GCTCTTCn'nnn_ Cuts at: 2528 2528 Size: 6139 SbfI CC_TGCA'GG Cuts at: 2157 3491 2157 Size: 1334 4805 ScaI AGT'ACT Cuts at: 4387 6099 4387 Size: 1712 4427 SfcI C'TryA_G Cuts at: 826 1045 2153 2792 3487 4622 5303 5494 826 Size: 219 1108 639 695 1135 681 191 1471 Fragments arranged by size: 1471 1135 1108 695 681 639 219 191 SfoI GGC'GCC Cuts at: 2586 2586 Size: 6139 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6139 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6139 SmaI CCC'GGG Cuts at: 1872 2034 2041 2700 3466 1872 Size: 162 7 659 766 4545 Fragments arranged by size: 4545 766 659 162 7 SmlI C'TyrA_G Cuts at: 820 1017 1943 2433 2441 4243 5144 5406 820 Size: 197 926 490 8 1802 901 262 1553 Fragments arranged by size: 1802 1553 926 901 490 262 197 8 SnaBI TAC'GTA Cuts at: 493 3930 493 Size: 3437 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6139 SphI G_CATG'C Cuts at: 1132 3497 1132 Size: 2365 3774 SspI AAT'ATT Cuts at: 5 52 4063 5 Size: 47 4011 2081 Fragments arranged by size: 4011 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6139 StyI C'CwwG_G Cuts at: 513 1065 2004 2574 2613 3355 3578 513 Size: 552 939 570 39 742 223 3074 Fragments arranged by size: 3074 939 742 570 552 223 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4166 4183 4336 4521 4966 325 Size: 1255 2586 17 153 185 445 1498 Fragments arranged by size: 2586 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2259 4385 6097 Size: 274 80 33 51 1443 282 2126 1712 Cuts at: 6097 96 Size: 138 Fragments arranged by size: 2126 1712 1443 282 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2940 2972 6099 6131 2940 Size: 32 3127 32 2948 Fragments arranged by size: 3127 2948 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2225 1316 Size: 909 5230 XbaI T'CTAG_A Cuts at: 3475 3475 Size: 6139 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2180 2323 3415 2180 Size: 143 1092 4904 Fragments arranged by size: 4904 1092 143 XhoI C'TCGA_G Cuts at: 1943 2433 2441 1943 Size: 490 8 5641 Fragments arranged by size: 5641 490 8 XmaI C'CCGG_G Cuts at: 1870 2032 2039 2698 3464 1870 Size: 162 7 659 766 4545 Fragments arranged by size: 4545 766 659 162 7 XmnI GAAnn'nnTTC Cuts at: 4268 4268 Size: 6139 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3947 276 Size: 53 83 186 936 2413 2468 Fragments arranged by size: 2468 2413 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PsrI SacI SexAI SfiI SrfI SwaI