(Circular) (Six-base) MAPSORT of: pFN21AE1040.seq Check: 143 from: 1 to: 6049 pFN21AE1040 6049 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2460 2460 Size: 6049 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3859 278 Size: 53 83 186 936 2323 2468 Fragments arranged by size: 2468 2323 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2345 3392 1937 Size: 408 1047 4594 Fragments arranged by size: 4594 1047 408 Acc65I G'GTAC_C Cuts at: 3370 3370 Size: 6049 AclI AA'CG_TT Cuts at: 1563 4176 4549 1563 Size: 2613 373 3063 Fragments arranged by size: 3063 2613 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2096 2432 2849 2990 3243 4112 5496 5764 Size: 464 336 417 141 253 869 1384 268 Cuts at: 5764 1632 Size: 1917 Fragments arranged by size: 1917 1384 869 464 417 336 268 253 141 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5852 AflIII A'CryG_T Cuts at: 1203 2053 3132 4948 5925 1203 Size: 850 1079 1816 977 1327 Fragments arranged by size: 1816 1327 1079 977 850 AgeI A'CCGG_T Cuts at: 4880 4880 Size: 6049 AhdI GACnn_n'nnGTC Cuts at: 3133 4778 3133 Size: 1645 4404 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6049 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2859 3275 1184 Size: 1675 416 3958 Fragments arranged by size: 3958 1675 416 AlwNI CAG_nnn'CTG Cuts at: 2341 2485 3792 5364 2341 Size: 144 1307 1572 3026 Fragments arranged by size: 3026 1572 1307 144 ApaLI G'TGCA_C Cuts at: 4106 5262 4106 Size: 1156 4893 ApoI r'AATT_y Cuts at: 1493 3358 3584 3736 5845 1493 Size: 1865 226 152 2109 1697 Fragments arranged by size: 2109 1865 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4603 160 Size: 872 3571 1606 Fragments arranged by size: 3571 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6049 AvaI C'yCGr_G Cuts at: 1870 1943 3008 3374 1870 Size: 73 1065 366 4545 Fragments arranged by size: 4545 1065 366 73 BamHI G'GATC_C Cuts at: 2289 2502 3379 3746 2289 Size: 213 877 367 4592 Fragments arranged by size: 4592 877 367 213 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3370 4825 618 Size: 325 217 627 1583 1455 1842 Fragments arranged by size: 1842 1583 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2012 3368 1391 Size: 621 1356 4072 Fragments arranged by size: 4072 1356 621 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5078 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2038 2072 2600 2634 4240 4274 1903 Size: 34 101 34 528 34 1606 34 3678 Fragments arranged by size: 3678 1606 528 101 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3941 5151 3941 Size: 1210 4839 BclI T'GATC_A Cuts at: 1548 1548 Size: 6049 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4660 136 Size: 107 122 71 631 605 2988 1525 Fragments arranged by size: 2988 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2095 6044 2095 Size: 3949 2100 BlpI GC'TnA_GC Cuts at: 2021 2045 2672 3466 2021 Size: 24 627 794 4604 Fragments arranged by size: 4604 794 627 24 Bme1580I G_kGCm'C Cuts at: 1790 4110 5266 1790 Size: 2320 1156 2573 Fragments arranged by size: 2573 2320 1156 BmgBI CAC'GTC Cuts at: 1318 2056 1318 Size: 738 5311 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2389 3335 3780 Size: 416 53 83 399 24 972 946 445 Cuts at: 3780 4738 442 Size: 958 1753 Fragments arranged by size: 1753 972 958 946 445 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6049 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2507 2528 4709 1239 Size: 1268 21 2181 2579 Fragments arranged by size: 2579 2181 1268 21 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3331 4174 5039 5337 3331 Size: 843 865 298 4043 Fragments arranged by size: 4043 865 843 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2272 3169 4712 882 Size: 617 773 897 1543 2219 Fragments arranged by size: 2219 1543 897 773 617 BsaAI yAC'GTr Cuts at: 493 1204 3133 3840 493 Size: 711 1929 707 2702 Fragments arranged by size: 2702 1929 711 707 BsaBI GATnn'nnATC Cuts at: 6043 6043 Size: 6049 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2873 3856 4238 4873 Size: 53 83 186 936 1340 983 382 635 Cuts at: 4873 275 Size: 1451 Fragments arranged by size: 1451 1340 983 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2124 2499 4482 4880 5154 5301 5712 2124 Size: 375 1983 398 274 147 411 2461 Fragments arranged by size: 2461 1983 411 398 375 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5824 Fragments arranged by size: 5824 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3227 1186 Size: 2041 4008 BseYI C'CCAG_C Cuts at: 3071 5252 5748 3071 Size: 2181 496 3372 Fragments arranged by size: 3372 2181 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5476 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2812 3499 4260 5288 1540 Size: 275 186 811 687 761 1028 2301 Fragments arranged by size: 2301 1028 811 761 687 275 186 BsiHKAI G_wGCw'C Cuts at: 2012 2493 4110 4195 5266 2012 Size: 481 1617 85 1071 2795 Fragments arranged by size: 2795 1617 1071 481 85 BsiWI C'GTAC_G Cuts at: 2919 2919 Size: 6049 BsmI GAATG_Cn' Cuts at: 3560 3653 3560 Size: 93 5956 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2493 3368 4110 4195 5266 1391 Size: 399 222 481 875 742 85 1071 2174 Fragments arranged by size: 2174 1071 875 742 481 399 222 85 BspEI T'CCGG_A Cuts at: 5712 5712 Size: 6049 BspHI T'CATG_A Cuts at: 3938 5668 3938 Size: 1730 4319 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2115 2460 3404 844 Size: 1271 345 944 3489 Fragments arranged by size: 3489 1271 944 345 BsrBI CCG'CTC Cuts at: 1394 1823 2025 3936 1394 Size: 429 202 1911 3507 Fragments arranged by size: 3507 1911 429 202 BsrDI GCAATG_nn' Cuts at: 65 1248 2676 4544 4718 65 Size: 1183 1428 1868 174 1396 Fragments arranged by size: 1868 1428 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2869 4693 4880 1700 Size: 110 1059 1824 187 2869 Fragments arranged by size: 2869 1824 1059 187 110 BsrGI T'GTAC_A Cuts at: 96 3099 96 Size: 3003 3046 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6049 BssSI C'ACGA_G Cuts at: 2215 4109 5121 2215 Size: 1894 1012 3143 Fragments arranged by size: 3143 1894 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6049 BstBI TT'CG_AA Cuts at: 4853 4853 Size: 6049 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6049 BstYI r'GATC_y Cuts at: 1972 2095 2289 2502 3294 3379 3746 4131 4148 Size: 123 194 213 792 85 367 385 17 Cuts at: 4148 5600 5686 5698 6044 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 792 385 367 346 213 194 123 86 85 17 12 BtgI C'CryG_G Cuts at: 513 1065 3024 3080 513 Size: 552 1959 56 3482 Fragments arranged by size: 3482 1959 552 56 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5310 Fragments arranged by size: 5310 683 56 BtsI GCAGTG_nn' Cuts at: 3565 4359 4379 3565 Size: 794 20 5235 Fragments arranged by size: 5235 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6049 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6014 DraI TTT'AAA Cuts at: 3353 3353 Size: 6049 DraIII CAC_nnn'GTG Cuts at: 2059 3329 2059 Size: 1270 4779 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3041 3161 5056 809 Size: 507 1725 120 1895 1802 Fragments arranged by size: 1895 1802 1725 507 120 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3496 4385 6014 Size: 54 1425 50 275 18 1666 889 1629 Cuts at: 6014 8 Size: 43 Fragments arranged by size: 1666 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3496 1537 Size: 275 1684 4090 Fragments arranged by size: 4090 1684 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2735 3058 3979 1353 Size: 1382 323 921 3423 Fragments arranged by size: 3423 1382 921 323 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4636 5010 5156 387 Size: 1064 138 3047 374 146 1280 Fragments arranged by size: 3047 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2010 2010 Size: 6049 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2432 2507 2528 2849 2990 3243 Size: 393 464 336 75 21 321 141 253 Cuts at: 3243 4112 4709 5496 5764 1239 Size: 869 597 787 268 1524 Fragments arranged by size: 1524 869 787 597 464 393 336 321 268 253 141 75 21 EcoNI CCTnn'n_nnAGG Cuts at: 3185 3185 Size: 6049 EcoO109I rG'GnC_Cy Cuts at: 3013 3319 3013 Size: 306 5743 EcoRI G'AATT_C Cuts at: 3358 3736 3358 Size: 378 5671 FspI TGC'GCA Cuts at: 4555 4555 Size: 6049 HaeII r_GCGC'y Cuts at: 1678 2087 5196 1678 Size: 409 3109 2531 Fragments arranged by size: 3109 2531 409 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2388 2420 2744 2776 3119 Size: 32 135 32 816 32 324 32 343 Cuts at: 3119 3151 4690 4722 4764 4796 1373 Size: 32 1539 32 42 32 2626 Fragments arranged by size: 2626 1539 816 343 324 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2401 3393 3639 669 Size: 988 281 463 992 246 3079 Fragments arranged by size: 3079 992 988 463 281 246 HindIII A'AGCT_T Cuts at: 748 2414 748 Size: 1666 4383 HpaI GTT'AAC Cuts at: 3639 3639 Size: 6049 KpnI G_GTAC'C Cuts at: 3374 3374 Size: 6049 MfeI C'AATT_G Cuts at: 3648 3648 Size: 6049 MluI A'CGCG_T Cuts at: 5925 5925 Size: 6049 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2493 2787 5163 5347 1305 Size: 1188 294 2376 184 2007 Fragments arranged by size: 2376 2007 1188 294 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4227 Fragments arranged by size: 4227 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2237 4007 4366 4525 518 Size: 648 1071 1770 359 159 2042 Fragments arranged by size: 2042 1770 1071 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2116 2482 2698 2992 3465 4142 5290 5535 Size: 510 366 216 294 473 677 1148 245 Cuts at: 5535 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 510 473 366 294 245 216 NaeI GCC'GGC Cuts at: 2871 2871 Size: 6049 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5497 NdeI CA'TA_TG Cuts at: 387 387 Size: 6049 NgoMIV G'CCGG_C Cuts at: 2869 2869 Size: 6049 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6049 NotI GC'GGCC_GC Cuts at: 3496 3496 Size: 6049 NspI r_CATG'y Cuts at: 1132 1229 3407 4952 1132 Size: 97 2178 1545 2229 Fragments arranged by size: 2229 2178 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6049 PciI A'CATG_T Cuts at: 4948 4948 Size: 6049 PflMI CCAn_nnn'nTGG Cuts at: 1108 2485 1108 Size: 1377 4672 PfoI T'CCnGG_A Cuts at: 2505 2547 2703 2712 2505 Size: 42 156 9 5842 Fragments arranged by size: 5842 156 42 9 PmeI GTTT'AAAC Cuts at: 3353 3353 Size: 6049 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4118 4150 5631 5663 4118 Size: 32 1481 32 4504 Fragments arranged by size: 4504 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3013 3319 3013 Size: 306 5743 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6049 PsiI TTA'TAA Cuts at: 3619 3619 Size: 6049 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3176 3208 3176 Size: 32 6017 PstI C_TGCA'G Cuts at: 830 3173 3401 830 Size: 2343 228 3478 Fragments arranged by size: 3478 2343 228 PvuI CG_AT'CG Cuts at: 2001 2812 2001 Size: 811 5238 PvuII CAG'CTG Cuts at: 2116 2116 Size: 6049 SacI G_AGCT'C Cuts at: 2012 2012 Size: 6049 SalI G'TCGA_C Cuts at: 1936 3391 1936 Size: 1455 4594 SbfI CC_TGCA'GG Cuts at: 3401 3401 Size: 6049 ScaI AGT'ACT Cuts at: 4297 6009 4297 Size: 1712 4337 SexAI A'CCwGG_T Cuts at: 3186 3186 Size: 6049 SfcI C'TryA_G Cuts at: 826 1045 2902 3169 3247 3397 4532 5213 5404 Size: 219 1857 267 78 150 1135 681 191 Cuts at: 5404 826 Size: 1471 Fragments arranged by size: 1857 1471 1135 681 267 219 191 150 78 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6049 SgrAI Cr'CCGG_yG Cuts at: 1700 2869 1700 Size: 1169 4880 SmaI CCC'GGG Cuts at: 1872 3376 1872 Size: 1504 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3310 4153 5054 5316 820 Size: 197 926 1367 843 901 262 1553 Fragments arranged by size: 1553 1367 926 901 843 262 197 SnaBI TAC'GTA Cuts at: 493 3840 493 Size: 3347 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6049 SphI G_CATG'C Cuts at: 1132 3407 1132 Size: 2275 3774 SspI AAT'ATT Cuts at: 5 52 3973 5 Size: 47 3921 2081 Fragments arranged by size: 3921 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6049 StyI C'CwwG_G Cuts at: 513 1065 3488 513 Size: 552 2423 3074 Fragments arranged by size: 3074 2423 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2822 3317 4076 4093 4246 4431 4876 Size: 1255 1242 495 759 17 153 185 445 Cuts at: 4876 325 Size: 1498 Fragments arranged by size: 1498 1255 1242 759 495 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3099 4295 6007 Size: 274 80 33 51 1443 1122 1196 1712 Cuts at: 6007 96 Size: 138 Fragments arranged by size: 1712 1443 1196 1122 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2377 2409 6009 6041 2377 Size: 32 3600 32 2385 Fragments arranged by size: 3600 2385 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6049 XbaI T'CTAG_A Cuts at: 2092 3385 2092 Size: 1293 4756 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3299 3299 Size: 6049 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6049 XmaI C'CCGG_G Cuts at: 1870 3374 1870 Size: 1504 4545 XmnI GAAnn'nnTTC Cuts at: 4178 4178 Size: 6049 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3857 276 Size: 53 83 186 936 2323 2468 Fragments arranged by size: 2468 2323 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI