################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BanI BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BsmI BspDI BspEI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Cac8I CelII Cfr10I ClaI CpoI DraI DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FbaI Fnu4HI FseI FspI HaeII Hin1I HpaI ItaI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI TseI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AccI 1303 GTCTAC GT^[AC][GT]AC AocI 1309 CCTAAGG CC^T[ACGT]AGG BbsI 1233 GAAGAC GAAGAC(2/6) BpmI 638 CTGGAG CTGGAG(16/14) BpuAI 1233 GAAGAC GAAGAC(2/6) BsgI 1497 GTGCAG GTGCAG(16/14) Bsp1407I 1371 TGTACA T^GTACA BspHI 83 TCATGA T^CATGA BsrGI 1371 TGTACA T^GTACA BstXI 903 CCATAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1309 CCTAAGG CC^T[ACGT]AGG CfoI 1698 GCGC GCG^C DraII 562 AGGCCCT [GA]G^G[ACGT]CC[CT] DraIII 194 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 1309 CCTAAGG CC^T[ACGT]AGG EcoO109I 562 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoT14I 313 CCAAGG C^C[AT][AT]GG FauI 18 CCCGC CCCGC(4/6) HgaI 1762 GACGC GACGC(5/10) HhaI 1698 GCGC GCG^C HinP1I 1698 GCGC G^CGC MwoI 864 GCTTGTTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PleI 25 GAGTC GAGTC(4/5) RcaI 83 TCATGA T^CATGA SapI 840 GAAGAGC GCTCTTC(1/4) SexAI 104 ACCTGGT A^CC[AT]GGT SspBI 1371 TGTACA T^GTACA StuI 1598 AGGCCT AGG^CCT StyI 313 CCAAGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AciI 19 CCGC CCGC(-3/-1) 432 CCGC AvaI 9 CCCGAG C^[CT]CG[GA]G 1614 CTCGGG AvaII 51 GGACC G^G[AT]CC 462 GGTCC BamHI 237 GGATCC G^GATCC 1049 GGATCC BsaI 1485 GAGACC GGTCTC(1/5) 108 GGTCTC BseRI 34 GAGGAG GAGGAG(10/8) 142 GAGGAG BsoBI 9 CCCGAG C^[CT]CG[GA]G 1614 CTCGGG BsrDI 860 CATTGC GCAATG(2/0) 491 GCAATG EarI 388 GAAGAG CTCTTC(1/4) 840 GAAGAG HincII 1691 GTCAAC GT[CT]^[GA]AC 1708 GTCAAC HindII 1691 GTCAAC GT[CT]^[GA]AC 1708 GTCAAC HindIII 1431 AAGCTT A^AGCTT 1515 AAGCTT Ksp632I 388 GAAGAG CTCTTC(1/4) 840 GAAGAG Tsp45I 746 GTGAC ^GT[GC]AC 1760 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI Asp700I AspHI BanII BmyI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII EcoRI EcoRII FokI HaeIII HapII HinfI HpaII HphI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI NdeII NlaIII NlaIV RsaI SacI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I Tsp509I TspRI TthHB8I XhoII XmnI