(Circular) (Six-base) MAPSORT of: pFN21AE3512.seq Check: 8258 from: 1 to: 6541 pFN21AE3512 6541 bp With 182 enzymes: SgfI * July 6, 2012 17:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4351 278 Size: 53 83 186 936 2815 2468 Fragments arranged by size: 2815 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3309 3884 1937 Size: 1372 575 4594 Fragments arranged by size: 4594 1372 575 Acc65I G'GTAC_C Cuts at: 3862 3862 Size: 6541 AclI AA'CG_TT Cuts at: 1563 4668 5041 1563 Size: 3105 373 3063 Fragments arranged by size: 3105 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2668 2752 3424 4604 5988 6256 1632 Size: 1036 84 672 1180 1384 268 1917 Fragments arranged by size: 1917 1384 1180 1036 672 268 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6344 AflIII A'CryG_T Cuts at: 1203 5440 6417 1203 Size: 4237 977 1327 Fragments arranged by size: 4237 1327 977 AgeI A'CCGG_T Cuts at: 5372 5372 Size: 6541 AhdI GACnn_n'nnGTC Cuts at: 5270 5270 Size: 6541 AleI CACnn'nnGTG Cuts at: 1166 2811 3399 3483 1166 Size: 1645 588 84 4224 Fragments arranged by size: 4224 1645 588 84 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6541 AlwNI CAG_nnn'CTG Cuts at: 4284 5856 4284 Size: 1572 4969 ApaLI G'TGCA_C Cuts at: 4598 5754 4598 Size: 1156 5385 ApoI r'AATT_y Cuts at: 1493 2550 2860 2886 2970 3138 3222 3850 4076 Size: 1057 310 26 84 168 84 628 226 Cuts at: 4076 4228 6337 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1057 628 310 226 168 152 84 84 26 AseI AT'TA_AT Cuts at: 160 1032 5095 160 Size: 872 4063 1606 Fragments arranged by size: 4063 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6541 AvaI C'yCGr_G Cuts at: 1870 1943 2014 3619 3866 1870 Size: 73 71 1605 247 4545 Fragments arranged by size: 4545 1605 247 73 71 BamHI G'GATC_C Cuts at: 2242 3054 3871 4238 2242 Size: 812 817 367 4545 Fragments arranged by size: 4545 817 812 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3862 5317 618 Size: 325 217 627 2075 1455 1842 Fragments arranged by size: 2075 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2021 2612 3032 3452 3860 1391 Size: 630 591 420 420 408 4072 Fragments arranged by size: 4072 630 591 420 420 408 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3245 928 Size: 971 1346 4224 Fragments arranged by size: 4224 1346 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4732 4766 1903 Size: 34 2795 34 3678 Fragments arranged by size: 3678 2795 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4433 5643 4433 Size: 1210 5331 BclI T'GATC_A Cuts at: 1548 1548 Size: 6541 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5152 136 Size: 107 122 71 631 605 3480 1525 Fragments arranged by size: 3480 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6536 6536 Size: 6541 BlpI GC'TnA_GC Cuts at: 3958 3958 Size: 6541 Bme1580I G_kGCm'C Cuts at: 1790 4602 5758 1790 Size: 2812 1156 2573 Fragments arranged by size: 2812 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6541 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2568 2904 4272 Size: 416 53 83 399 24 1151 336 1368 Cuts at: 4272 5230 442 Size: 958 1753 Fragments arranged by size: 1753 1368 1151 958 416 399 336 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6541 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2664 5201 1239 Size: 1425 2537 2579 Fragments arranged by size: 2579 2537 1425 Bpu10I CC'TnA_GC Cuts at: 3199 3199 Size: 6541 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4666 5531 5829 4666 Size: 865 298 5378 Fragments arranged by size: 5378 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2119 3484 5204 882 Size: 617 620 1365 1720 2219 Fragments arranged by size: 2219 1720 1365 620 617 BsaAI yAC'GTr Cuts at: 493 1204 4332 493 Size: 711 3128 2702 Fragments arranged by size: 3128 2702 711 BsaBI GATnn'nnATC Cuts at: 6535 6535 Size: 6541 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4348 4730 5365 275 Size: 53 83 186 936 2815 382 635 1451 Fragments arranged by size: 2815 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4974 5372 5646 5793 6204 4974 Size: 398 274 147 411 5311 Fragments arranged by size: 5311 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6316 Fragments arranged by size: 6316 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2054 2162 1186 Size: 868 108 5565 Fragments arranged by size: 5565 868 108 BseYI C'CCAG_C Cuts at: 2059 5744 6240 2059 Size: 3685 496 2360 Fragments arranged by size: 3685 2360 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3523 1163 Size: 573 1787 4181 Fragments arranged by size: 4181 1787 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3991 4752 5780 1540 Size: 275 186 1990 761 1028 2301 Fragments arranged by size: 2301 1990 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2612 3032 3370 3452 4602 4687 5758 2612 Size: 420 338 82 1150 85 1071 3395 Fragments arranged by size: 3395 1150 1071 420 338 85 82 BsmI GAATG_Cn' Cuts at: 4052 4145 4052 Size: 93 6448 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2612 3032 3370 3452 3860 4602 Size: 399 231 591 420 338 82 408 742 Cuts at: 4602 4687 5758 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 591 420 408 399 338 231 85 82 BspEI T'CCGG_A Cuts at: 6204 6204 Size: 6541 BspHI T'CATG_A Cuts at: 2088 4430 6160 2088 Size: 2342 1730 2469 Fragments arranged by size: 2469 2342 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3896 844 Size: 3052 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4428 1394 Size: 429 2605 3507 Fragments arranged by size: 3507 2605 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2503 2864 5036 5210 65 Size: 1183 1255 361 2172 174 1396 Fragments arranged by size: 2172 1396 1255 1183 361 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5185 5372 1700 Size: 110 3375 187 2869 Fragments arranged by size: 3375 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3376 96 Size: 3280 3261 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6541 BssSI C'ACGA_G Cuts at: 4601 5613 4601 Size: 1012 5529 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6541 BstBI TT'CG_AA Cuts at: 5345 5345 Size: 6541 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6541 BstXI CCAn_nnnn'nTGG Cuts at: 2915 2915 Size: 6541 BstYI r'GATC_y Cuts at: 1972 2242 2298 3054 3726 3871 4238 4623 4640 Size: 270 56 756 672 145 367 385 17 Cuts at: 4640 6092 6178 6190 6536 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 756 672 385 367 346 270 145 86 56 17 12 Bsu36I CC'TnA_GG Cuts at: 3315 3315 Size: 6541 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5050 Fragments arranged by size: 5050 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5802 Fragments arranged by size: 5802 683 56 BtsI GCAGTG_nn' Cuts at: 4057 4851 4871 4057 Size: 794 20 5727 Fragments arranged by size: 5727 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6541 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2485 2520 3073 3108 529 Size: 35 1921 35 553 35 3962 Fragments arranged by size: 3962 1921 553 35 35 35 DraI TTT'AAA Cuts at: 3845 3845 Size: 6541 DraIII CAC_nnn'GTG Cuts at: 2204 2204 Size: 6541 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5548 809 Size: 507 4232 1802 Fragments arranged by size: 4232 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3988 4877 6506 Size: 54 1425 50 275 18 2158 889 1629 Cuts at: 6506 8 Size: 43 Fragments arranged by size: 2158 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3988 1537 Size: 275 2176 4090 Fragments arranged by size: 4090 2176 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2388 2840 4471 1353 Size: 1035 452 1631 3423 Fragments arranged by size: 3423 1631 1035 452 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5128 5502 5648 387 Size: 1064 138 3539 374 146 1280 Fragments arranged by size: 3539 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2610 3030 3450 2610 Size: 420 420 5701 Fragments arranged by size: 5701 420 420 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2664 2668 2752 3424 4604 5201 5988 Size: 393 1032 4 84 672 1180 597 787 Cuts at: 5988 6256 1239 Size: 268 1524 Fragments arranged by size: 1524 1180 1032 787 672 597 393 268 84 4 EcoO109I rG'GnC_Cy Cuts at: 2568 2568 Size: 6541 EcoRI G'AATT_C Cuts at: 2550 2886 3138 3222 3850 4228 2550 Size: 336 252 84 628 378 4863 Fragments arranged by size: 4863 628 378 336 252 84 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2343 2866 2343 Size: 523 6018 FspI TGC'GCA Cuts at: 5047 5047 Size: 6541 HaeII r_GCGC'y Cuts at: 1678 5688 1678 Size: 4010 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2839 2871 3481 3513 5182 Size: 32 135 32 1267 32 610 32 1669 Cuts at: 5182 5214 5256 5288 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1669 1267 610 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3698 3715 3885 4131 669 Size: 988 281 1760 17 170 246 3079 Fragments arranged by size: 3079 1760 988 281 246 170 17 HindIII A'AGCT_T Cuts at: 748 3436 3520 748 Size: 2688 84 3769 Fragments arranged by size: 3769 2688 84 HpaI GTT'AAC Cuts at: 4131 4131 Size: 6541 KpnI G_GTAC'C Cuts at: 3866 3866 Size: 6541 MfeI C'AATT_G Cuts at: 4140 4140 Size: 6541 MluI A'CGCG_T Cuts at: 6417 6417 Size: 6541 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2753 3005 5655 5839 1305 Size: 1448 252 2650 184 2007 Fragments arranged by size: 2650 2007 1448 252 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4719 Fragments arranged by size: 4719 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2409 2475 2727 2811 2913 3063 3165 Size: 648 1243 66 252 84 102 150 102 Cuts at: 3165 3399 3483 3555 3585 3762 4499 4858 5017 Size: 234 84 72 30 177 737 359 159 Cuts at: 5017 518 Size: 2042 Fragments arranged by size: 2042 1243 737 648 359 252 234 177 159 150 102 102 84 84 72 66 30 MspA1I CmG'CkG Cuts at: 1606 3957 4634 5782 6027 1606 Size: 2351 677 1148 245 2120 Fragments arranged by size: 2351 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5050 Fragments arranged by size: 5050 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6541 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6541 NotI GC'GGCC_GC Cuts at: 3988 3988 Size: 6541 NspI r_CATG'y Cuts at: 1132 1229 3899 5444 1132 Size: 97 2670 1545 2229 Fragments arranged by size: 2670 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6541 PciI A'CATG_T Cuts at: 5440 5440 Size: 6541 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6541 PmeI GTTT'AAAC Cuts at: 3845 3845 Size: 6541 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4610 4642 6123 6155 4610 Size: 32 1481 32 4996 Fragments arranged by size: 4996 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6541 PsiI TTA'TAA Cuts at: 3330 3666 4111 3330 Size: 336 445 5760 Fragments arranged by size: 5760 445 336 PstI C_TGCA'G Cuts at: 830 3893 830 Size: 3063 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6541 SacI G_AGCT'C Cuts at: 2612 3032 3452 2612 Size: 420 420 5701 Fragments arranged by size: 5701 420 420 SalI G'TCGA_C Cuts at: 1936 3883 1936 Size: 1947 4594 SapI GCTCTTCn'nnn_ Cuts at: 2840 2840 Size: 6541 SbfI CC_TGCA'GG Cuts at: 3893 3893 Size: 6541 ScaI AGT'ACT Cuts at: 4789 6501 4789 Size: 1712 4829 SexAI A'CCwGG_T Cuts at: 2109 2109 Size: 6541 SfcI C'TryA_G Cuts at: 826 1045 3889 5024 5705 5896 826 Size: 219 2844 1135 681 191 1471 Fragments arranged by size: 2844 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6541 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6541 SmaI CCC'GGG Cuts at: 1872 3868 1872 Size: 1996 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4645 5546 5808 820 Size: 197 926 2702 901 262 1553 Fragments arranged by size: 2702 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4332 493 Size: 3839 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6541 SphI G_CATG'C Cuts at: 1132 3899 1132 Size: 2767 3774 SspI AAT'ATT Cuts at: 5 52 4465 5 Size: 47 4413 2081 Fragments arranged by size: 4413 2081 47 StuI AGG'CCT Cuts at: 1517 3605 1517 Size: 2088 4453 StyI C'CwwG_G Cuts at: 513 1065 2004 2318 3980 513 Size: 552 939 314 1662 3074 Fragments arranged by size: 3074 1662 939 552 314 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4568 4585 4738 4923 5368 325 Size: 1255 2988 17 153 185 445 1498 Fragments arranged by size: 2988 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3376 3419 4787 Size: 274 80 33 51 1443 1399 43 1368 Cuts at: 4787 6499 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1399 1368 274 138 80 51 43 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6501 6533 6501 Size: 32 6509 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6541 XbaI T'CTAG_A Cuts at: 3877 3877 Size: 6541 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6541 XmaI C'CCGG_G Cuts at: 1870 3866 1870 Size: 1996 4545 XmnI GAAnn'nnTTC Cuts at: 2765 3101 3437 4670 2765 Size: 336 336 1233 4636 Fragments arranged by size: 4636 1233 336 336 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4349 276 Size: 53 83 186 936 2815 2468 Fragments arranged by size: 2815 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI