(Circular) (Six-base) MAPSORT of: pFN21AE1036.seq Check: 4673 from: 1 to: 6379 pFN21AE1036 6379 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2318 2318 Size: 6379 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4189 278 Size: 53 83 186 936 2653 2468 Fragments arranged by size: 2653 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2478 3722 1937 Size: 541 1244 4594 Fragments arranged by size: 4594 1244 541 Acc65I G'GTAC_C Cuts at: 3700 3700 Size: 6379 AclI AA'CG_TT Cuts at: 1563 4506 4879 1563 Size: 2943 373 3063 Fragments arranged by size: 3063 2943 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2181 2406 3377 4442 5826 6094 1632 Size: 549 225 971 1065 1384 268 1917 Fragments arranged by size: 1917 1384 1065 971 549 268 225 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6182 AflIII A'CryG_T Cuts at: 1203 5278 6255 1203 Size: 4075 977 1327 Fragments arranged by size: 4075 1327 977 AgeI A'CCGG_T Cuts at: 5210 5210 Size: 6379 AhdI GACnn_n'nnGTC Cuts at: 3328 5108 3328 Size: 1780 4599 AleI CACnn'nnGTG Cuts at: 1166 2963 1166 Size: 1797 4582 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2981 1184 Size: 1797 4582 AlwNI CAG_nnn'CTG Cuts at: 2114 2133 2678 2845 4122 5694 2114 Size: 19 545 167 1277 1572 2799 Fragments arranged by size: 2799 1572 1277 545 167 19 ApaI G_GGCC'C Cuts at: 2080 2528 3531 2080 Size: 448 1003 4928 Fragments arranged by size: 4928 1003 448 ApaLI G'TGCA_C Cuts at: 4436 5592 4436 Size: 1156 5223 ApoI r'AATT_y Cuts at: 1493 3688 3914 4066 6175 1493 Size: 2195 226 152 2109 1697 Fragments arranged by size: 2195 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4933 160 Size: 872 3901 1606 Fragments arranged by size: 3901 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6379 AvaI C'yCGr_G Cuts at: 1870 1943 2517 3381 3704 1870 Size: 73 574 864 323 4545 Fragments arranged by size: 4545 864 574 323 73 AvrII C'CTAG_G Cuts at: 2029 2650 2029 Size: 621 5758 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3247 3280 3247 Size: 33 6346 BamHI G'GATC_C Cuts at: 3709 4076 3709 Size: 367 6012 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2284 2655 2754 2965 3154 Size: 325 217 627 497 371 99 211 189 Cuts at: 3154 3672 3700 5155 618 Size: 518 28 1455 1842 Fragments arranged by size: 1842 1455 627 518 497 371 325 217 211 189 99 28 BanII G_rGCy'C Cuts at: 1391 2080 2528 2565 2832 3531 3698 1391 Size: 689 448 37 267 699 167 4072 Fragments arranged by size: 4072 699 689 448 267 167 37 BbeI G_GCGC'C Cuts at: 2659 2758 2659 Size: 99 6280 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2412 3395 928 Size: 971 513 983 3912 Fragments arranged by size: 3912 983 971 513 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4570 4604 1903 Size: 34 2633 34 3678 Fragments arranged by size: 3678 2633 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2131 2679 3387 4271 5481 2131 Size: 548 708 884 1210 3029 Fragments arranged by size: 3029 1210 884 708 548 BclI T'GATC_A Cuts at: 1548 1548 Size: 6379 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2346 3092 4990 Size: 107 122 71 631 605 674 746 1898 Cuts at: 4990 136 Size: 1525 Fragments arranged by size: 1898 1525 746 674 631 605 122 107 71 BglII A'GATC_T Cuts at: 6374 6374 Size: 6379 BlpI GC'TnA_GC Cuts at: 2018 2826 3796 2018 Size: 808 970 4601 Fragments arranged by size: 4601 970 808 Bme1580I G_kGCm'C Cuts at: 1790 2080 2161 2528 3159 3531 4440 5596 1790 Size: 290 81 367 631 372 909 1156 2573 Fragments arranged by size: 2573 1156 909 631 372 367 290 81 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6379 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2365 2497 4110 Size: 416 53 83 399 24 948 132 1613 Cuts at: 4110 5068 442 Size: 958 1753 Fragments arranged by size: 1753 1613 958 948 416 399 132 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6379 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2083 3451 3469 2083 Size: 1368 18 4993 Fragments arranged by size: 4993 1368 18 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2183 3208 3464 3562 5039 1239 Size: 944 1025 256 98 1477 2579 Fragments arranged by size: 2579 1477 1025 944 256 98 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2287 4504 5369 5667 2287 Size: 2217 865 298 2999 Fragments arranged by size: 2999 2217 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5042 882 Size: 617 3543 2219 Fragments arranged by size: 3543 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2142 3090 4170 493 Size: 711 938 948 1080 2702 Fragments arranged by size: 2702 1080 948 938 711 BsaBI GATnn'nnATC Cuts at: 6373 6373 Size: 6379 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2099 2656 2755 4186 Size: 53 83 186 936 566 557 99 1431 Cuts at: 4186 4568 5203 275 Size: 382 635 1451 Fragments arranged by size: 1451 1431 936 635 566 557 382 186 99 83 53 BsaWI w'CCGG_w Cuts at: 2463 3279 4812 5210 5484 5631 6042 2463 Size: 816 1533 398 274 147 411 2800 Fragments arranged by size: 2800 1533 816 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3233 3263 1178 Size: 30 165 30 1830 30 4294 Fragments arranged by size: 4294 1830 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2215 2582 3410 3424 1186 Size: 1029 367 828 14 4141 Fragments arranged by size: 4141 1029 828 367 14 BseYI C'CCAG_C Cuts at: 2303 2498 2870 3646 5582 6078 2303 Size: 195 372 776 1936 496 2604 Fragments arranged by size: 2604 1936 776 496 372 195 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2552 3271 3585 3596 3619 1163 Size: 573 816 719 314 11 23 3923 Fragments arranged by size: 3923 816 719 573 314 23 11 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3829 4590 5618 1540 Size: 275 186 1828 761 1028 2301 Fragments arranged by size: 2301 1828 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4440 4525 5596 4440 Size: 85 1071 5223 Fragments arranged by size: 5223 1071 85 BsmI GAATG_Cn' Cuts at: 2872 3890 3983 2872 Size: 1018 93 5268 Fragments arranged by size: 5268 1018 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2090 2899 2090 Size: 809 5570 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2080 2161 2528 2565 2832 3159 3531 Size: 399 290 81 367 37 267 327 372 Cuts at: 3531 3698 4440 4525 5596 1391 Size: 167 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 372 367 327 290 267 167 85 81 37 BspEI T'CCGG_A Cuts at: 2463 3279 6042 2463 Size: 816 2763 2800 Fragments arranged by size: 2800 2763 816 BspHI T'CATG_A Cuts at: 4268 5998 4268 Size: 1730 4649 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2318 2694 3734 844 Size: 1474 376 1040 3489 Fragments arranged by size: 3489 1474 1040 376 BsrBI CCG'CTC Cuts at: 1394 1823 3507 4266 1394 Size: 429 1684 759 3507 Fragments arranged by size: 3507 1684 759 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2309 2791 3349 4874 5048 65 Size: 1183 1061 482 558 1525 174 1396 Fragments arranged by size: 1525 1396 1183 1061 558 482 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2376 2811 5023 5210 1700 Size: 110 566 435 2212 187 2869 Fragments arranged by size: 2869 2212 566 435 187 110 BsrGI T'GTAC_A Cuts at: 96 2250 96 Size: 2154 4225 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6379 BssSI C'ACGA_G Cuts at: 4439 5451 4439 Size: 1012 5367 BstAPI GCAn_nnn'nTGC Cuts at: 794 2114 794 Size: 1320 5059 BstBI TT'CG_AA Cuts at: 5183 5183 Size: 6379 BstEII G'GTnAC_C Cuts at: 1184 3199 1184 Size: 2015 4364 BstXI CCAn_nnnn'nTGG Cuts at: 2635 2806 2905 3001 3130 3186 2635 Size: 171 99 96 129 56 5828 Fragments arranged by size: 5828 171 129 99 96 56 BstYI r'GATC_y Cuts at: 1972 3709 4076 4461 4478 5930 6016 6028 6374 Size: 1737 367 385 17 1452 86 12 346 Cuts at: 6374 1972 Size: 1977 Fragments arranged by size: 1977 1737 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3318 3318 Size: 6379 BtgI C'CryG_G Cuts at: 513 1065 2004 2994 3309 513 Size: 552 939 990 315 3583 Fragments arranged by size: 3583 990 939 552 315 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5640 Fragments arranged by size: 5640 683 56 BtsI GCAGTG_nn' Cuts at: 2112 3081 3425 3631 3895 4689 4709 2112 Size: 969 344 206 264 794 20 3782 Fragments arranged by size: 3782 969 794 344 264 206 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6379 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6344 DraI TTT'AAA Cuts at: 3683 3683 Size: 6379 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5386 809 Size: 507 4070 1802 Fragments arranged by size: 4070 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2082 2353 2471 Size: 54 1425 50 275 18 252 271 118 Cuts at: 2471 3826 4715 6344 8 Size: 1355 889 1629 43 Fragments arranged by size: 1629 1425 1355 889 275 271 252 118 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3826 1537 Size: 275 2014 4090 Fragments arranged by size: 4090 2014 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2736 3474 4309 1353 Size: 1383 738 835 3423 Fragments arranged by size: 3423 1383 835 738 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4966 5340 5486 387 Size: 1064 138 3377 374 146 1280 Fragments arranged by size: 3377 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2181 2183 2406 3208 3377 3464 3562 Size: 393 549 2 223 802 169 87 98 Cuts at: 3562 4442 5039 5826 6094 1239 Size: 880 597 787 268 1524 Fragments arranged by size: 1524 880 802 787 597 549 393 268 223 169 98 87 2 EcoO109I rG'GnC_Cy Cuts at: 2033 2076 2077 2524 2525 2698 3193 3263 2033 Size: 43 1 447 1 173 495 70 5149 Fragments arranged by size: 5149 495 447 173 70 43 1 1 EcoRI G'AATT_C Cuts at: 3688 4066 3688 Size: 378 6001 FspI TGC'GCA Cuts at: 4885 4885 Size: 6379 HaeII r_GCGC'y Cuts at: 1678 2659 2758 5526 1678 Size: 981 99 2768 2531 Fragments arranged by size: 2768 2531 981 99 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2653 2685 2907 2939 3205 Size: 32 135 32 1081 32 222 32 266 Cuts at: 3205 3237 3314 3346 5020 5052 5094 5126 1373 Size: 32 77 32 1674 32 42 32 2626 Fragments arranged by size: 2626 1674 1081 266 222 135 77 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2479 3723 3969 669 Size: 988 281 541 1244 246 3079 Fragments arranged by size: 3079 1244 988 541 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6379 HpaI GTT'AAC Cuts at: 3969 3969 Size: 6379 KasI G'GCGC_C Cuts at: 2655 2754 2655 Size: 99 6280 KpnI G_GTAC'C Cuts at: 3704 3704 Size: 6379 MfeI C'AATT_G Cuts at: 3978 3978 Size: 6379 MluI A'CGCG_T Cuts at: 6255 6255 Size: 6379 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3357 3405 5493 5677 1305 Size: 2052 48 2088 184 2007 Fragments arranged by size: 2088 2052 2007 184 48 MscI TGG'CCA Cuts at: 10 64 1489 1832 2473 10 Size: 54 1425 343 641 3916 Fragments arranged by size: 3916 1425 641 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2708 2804 2963 2999 4337 4696 4855 Size: 648 1542 96 159 36 1338 359 159 Cuts at: 4855 518 Size: 2042 Fragments arranged by size: 2042 1542 1338 648 359 159 159 96 36 MspA1I CmG'CkG Cuts at: 1606 2358 2825 3186 3247 3442 3583 3795 4472 Size: 752 467 361 61 195 141 212 677 Cuts at: 4472 5620 5865 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 752 677 467 361 245 212 195 141 61 NarI GG'CG_CC Cuts at: 2656 2755 2656 Size: 99 6280 NcoI C'CATG_G Cuts at: 513 1065 2004 2994 513 Size: 552 939 990 3898 Fragments arranged by size: 3898 990 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6379 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6379 NotI GC'GGCC_GC Cuts at: 3826 3826 Size: 6379 NspI r_CATG'y Cuts at: 1132 1229 2174 3737 5282 1132 Size: 97 945 1563 1545 2229 Fragments arranged by size: 2229 1563 1545 945 97 PasI CC'CwG_GG Cuts at: 1348 2833 1348 Size: 1485 4894 PciI A'CATG_T Cuts at: 5278 5278 Size: 6379 PflMI CCAn_nnn'nTGG Cuts at: 1108 2133 3028 1108 Size: 1025 895 4459 Fragments arranged by size: 4459 1025 895 PfoI T'CCnGG_A Cuts at: 3129 3129 Size: 6379 PmeI GTTT'AAAC Cuts at: 3683 3683 Size: 6379 PmlI CAC'GTG Cuts at: 3090 3090 Size: 6379 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4448 4480 5961 5993 4448 Size: 32 1481 32 4834 Fragments arranged by size: 4834 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3263 3263 Size: 6379 PshAI GACnn'nnGTC Cuts at: 1613 3489 1613 Size: 1876 4503 PsiI TTA'TAA Cuts at: 3949 3949 Size: 6379 PspOMI G'GGCC_C Cuts at: 2076 2524 3527 2076 Size: 448 1003 4928 Fragments arranged by size: 4928 1003 448 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2366 2398 2366 Size: 32 6347 PstI C_TGCA'G Cuts at: 830 2694 2721 3369 3731 830 Size: 1864 27 648 362 3478 Fragments arranged by size: 3478 1864 648 362 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6379 PvuII CAG'CTG Cuts at: 2825 3186 3247 3442 3583 2825 Size: 361 61 195 141 5621 Fragments arranged by size: 5621 361 195 141 61 SalI G'TCGA_C Cuts at: 1936 2477 3721 1936 Size: 541 1244 4594 Fragments arranged by size: 4594 1244 541 SbfI CC_TGCA'GG Cuts at: 3731 3731 Size: 6379 ScaI AGT'ACT Cuts at: 4627 6339 4627 Size: 1712 4667 SexAI A'CCwGG_T Cuts at: 2634 3150 2634 Size: 516 5863 SfcI C'TryA_G Cuts at: 826 1045 2690 2717 3365 3727 4862 5543 5734 Size: 219 1645 27 648 362 1135 681 191 Cuts at: 5734 826 Size: 1471 Fragments arranged by size: 1645 1471 1135 681 648 362 219 191 27 SfoI GGC'GCC Cuts at: 2657 2756 2657 Size: 99 6280 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6379 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6379 SmaI CCC'GGG Cuts at: 1872 3383 3706 1872 Size: 1511 323 4545 Fragments arranged by size: 4545 1511 323 SmlI C'TyrA_G Cuts at: 820 1017 1943 2266 2517 4483 5384 5646 820 Size: 197 926 323 251 1966 901 262 1553 Fragments arranged by size: 1966 1553 926 901 323 262 251 197 SnaBI TAC'GTA Cuts at: 493 2142 4170 493 Size: 1649 2028 2702 Fragments arranged by size: 2702 2028 1649 SpeI A'CTAG_T Cuts at: 152 152 Size: 6379 SphI G_CATG'C Cuts at: 1132 3737 1132 Size: 2605 3774 SspI AAT'ATT Cuts at: 5 52 4303 5 Size: 47 4251 2081 Fragments arranged by size: 4251 2081 47 StuI AGG'CCT Cuts at: 1517 3431 1517 Size: 1914 4465 StyI C'CwwG_G Cuts at: 513 1065 2004 2029 2650 2994 3159 3288 3818 Size: 552 939 25 621 344 165 129 530 Cuts at: 3818 513 Size: 3074 Fragments arranged by size: 3074 939 621 552 530 344 165 129 25 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4406 4423 4576 4761 5206 325 Size: 1255 2826 17 153 185 445 1498 Fragments arranged by size: 2826 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2250 3268 4625 Size: 274 80 33 51 1443 273 1018 1357 Cuts at: 4625 6337 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1357 1018 274 273 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3233 3265 3298 3330 6339 6371 3233 Size: 32 33 32 3009 32 3241 Fragments arranged by size: 3241 3009 33 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6379 XbaI T'CTAG_A Cuts at: 3715 3715 Size: 6379 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2635 3001 3025 2635 Size: 366 24 5989 Fragments arranged by size: 5989 366 24 XhoI C'TCGA_G Cuts at: 1943 2517 1943 Size: 574 5805 XmaI C'CCGG_G Cuts at: 1870 3381 3704 1870 Size: 1511 323 4545 Fragments arranged by size: 4545 1511 323 XmnI GAAnn'nnTTC Cuts at: 4508 4508 Size: 6379 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4187 276 Size: 53 83 186 936 2653 2468 Fragments arranged by size: 2653 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BbvCI BfrBI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI