Restriction map for
pFN21AE1036
(Linear) (Six-base) MAPSORT of: KIEE1036.seq Check: 5568 from: 1 to: 1674
KIEE1036.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 313 1674
Size: 313 1361
AccI GT'mk_AC
Cuts at: 0 473 1674
Size: 473 1201
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 401 1372 1674
Size: 176 225 971 302
Fragments arranged by size:
971 302 225 176
AhdI GACnn_n'nnGTC
Cuts at: 0 1323 1674
Size: 1323 351
AleI CACnn'nnGTG
Cuts at: 0 958 1674
Size: 958 716
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 976 1674
Size: 976 698
AlwNI CAG_nnn'CTG
Cuts at: 0 109 128 673 840 1674
Size: 109 19 545 167 834
Fragments arranged by size:
834 545 167 109 19
ApaI G_GGCC'C
Cuts at: 0 75 523 1526 1674
Size: 75 448 1003 148
Fragments arranged by size:
1003 448 148 75
AvaI C'yCGr_G
Cuts at: 0 512 1376 1674
Size: 512 864 298
Fragments arranged by size:
864 512 298
AvrII C'CTAG_G
Cuts at: 0 24 645 1674
Size: 24 621 1029
Fragments arranged by size:
1029 621 24
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1242 1275 1674
Size: 1242 33 399
Fragments arranged by size:
1242 399 33
BanI G'GyrC_C
Cuts at: 0 279 650 749 960 1149 1667 1674
Size: 279 371 99 211 189 518 7
Fragments arranged by size:
518 371 279 211 189 99 7
BanII G_rGCy'C
Cuts at: 0 75 523 560 827 1526 1674
Size: 75 448 37 267 699 148
Fragments arranged by size:
699 448 267 148 75 37
BbeI G_GCGC'C
Cuts at: 0 654 753 1674
Size: 654 99 921
Fragments arranged by size:
921 654 99
BbsI GAAGACnn'nnnn_
Cuts at: 0 407 1390 1674
Size: 407 983 284
Fragments arranged by size:
983 407 284
BciVI GTATCCnnnnn_n'
Cuts at: 0 126 674 1382 1674
Size: 126 548 708 292
Fragments arranged by size:
708 548 292 126
BglI GCCn_nnn'nGGC
Cuts at: 0 341 1087 1674
Size: 341 746 587
Fragments arranged by size:
746 587 341
BlpI GC'TnA_GC
Cuts at: 0 13 821 1674
Size: 13 808 853
Fragments arranged by size:
853 808 13
Bme1580I G_kGCm'C
Cuts at: 0 75 156 523 1154 1526 1674
Size: 75 81 367 631 372 148
Fragments arranged by size:
631 372 367 148 81 75
BmrI ACTGGGnnnn_n'
Cuts at: 0 360 492 1674
Size: 360 132 1182
Fragments arranged by size:
1182 360 132
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 78 1446 1464 1674
Size: 78 1368 18 210
Fragments arranged by size:
1368 210 78 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 1203 1459 1557 1674
Size: 178 1025 256 98 117
Fragments arranged by size:
1025 256 178 117 98
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 1674
Size: 282 1392
BsaAI yAC'GTr
Cuts at: 0 137 1085 1674
Size: 137 948 589
Fragments arranged by size:
948 589 137
BsaHI Gr'CG_yC
Cuts at: 0 94 651 750 1674
Size: 94 557 99 924
Fragments arranged by size:
924 557 99 94
BsaWI w'CCGG_w
Cuts at: 0 458 1274 1674
Size: 458 816 400
Fragments arranged by size:
816 458 400
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1228 1258 1674
Size: 1228 30 416
Fragments arranged by size:
1228 416 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 210 577 1405 1419 1674
Size: 210 367 828 14 255
Fragments arranged by size:
828 367 255 210 14
BseYI C'CCAG_C
Cuts at: 0 298 493 865 1641 1674
Size: 298 195 372 776 33
Fragments arranged by size:
776 372 298 195 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 1266 1580 1591 1614 1674
Size: 547 719 314 11 23 60
Fragments arranged by size:
719 547 314 60 23 11
BsmI GAATG_Cn'
Cuts at: 0 867 1674
Size: 867 807
BsmBI CGTCTCn'nnnn_
Cuts at: 0 85 894 1674
Size: 85 809 780
Fragments arranged by size:
809 780 85
Bsp1286I G_dGCh'C
Cuts at: 0 75 156 523 560 827 1154 1526 1674
Size: 75 81 367 37 267 327 372 148
Fragments arranged by size:
372 367 327 267 148 81 75 37
BspEI T'CCGG_A
Cuts at: 0 458 1274 1674
Size: 458 816 400
Fragments arranged by size:
816 458 400
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 313 689 1674
Size: 313 376 985
Fragments arranged by size:
985 376 313
BsrBI CCG'CTC
Cuts at: 0 1502 1674
Size: 1502 172
BsrDI GCAATG_nn'
Cuts at: 0 304 786 1344 1674
Size: 304 482 558 330
Fragments arranged by size:
558 482 330 304
BsrFI r'CCGG_y
Cuts at: 0 371 806 1674
Size: 371 435 868
Fragments arranged by size:
868 435 371
BsrGI T'GTAC_A
Cuts at: 0 245 1674
Size: 245 1429
BstAPI GCAn_nnn'nTGC
Cuts at: 0 109 1674
Size: 109 1565
BstEII G'GTnAC_C
Cuts at: 0 1194 1674
Size: 1194 480
BstXI CCAn_nnnn'nTGG
Cuts at: 0 630 801 900 996 1125 1181 1674
Size: 630 171 99 96 129 56 493
Fragments arranged by size:
630 493 171 129 99 96 56
Bsu36I CC'TnA_GG
Cuts at: 0 1313 1674
Size: 1313 361
BtgI C'CryG_G
Cuts at: 0 989 1304 1674
Size: 989 315 370
Fragments arranged by size:
989 370 315
BtsI GCAGTG_nn'
Cuts at: 0 107 1076 1420 1626 1674
Size: 107 969 344 206 48
Fragments arranged by size:
969 344 206 107 48
EaeI y'GGCC_r
Cuts at: 0 77 348 466 1674
Size: 77 271 118 1208
Fragments arranged by size:
1208 271 118 77
EarI CTCTTCn'nnn_
Cuts at: 0 731 1469 1674
Size: 731 738 205
Fragments arranged by size:
738 731 205
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 178 401 1203 1372 1459 1557 1674
Size: 176 2 223 802 169 87 98 117
Fragments arranged by size:
802 223 176 169 117 98 87 2
EcoO109I rG'GnC_Cy
Cuts at: 0 28 71 72 519 520 693 1188 1258
Size: 28 43 1 447 1 173 495 70
Cuts at: 1258 1674
Size: 416
Fragments arranged by size:
495 447 416 173 70 43 28 1
1
HaeII r_GCGC'y
Cuts at: 0 654 753 1674
Size: 654 99 921
Fragments arranged by size:
921 654 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 648 680 902 934 1200 1232 1309 1341
Size: 648 32 222 32 266 32 77 32
Cuts at: 1341 1674
Size: 333
Fragments arranged by size:
648 333 266 222 77 32 32 32
32
HincII GTy'rAC
Cuts at: 0 474 1674
Size: 474 1200
KasI G'GCGC_C
Cuts at: 0 650 749 1674
Size: 650 99 925
Fragments arranged by size:
925 650 99
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1352 1400 1674
Size: 1352 48 274
Fragments arranged by size:
1352 274 48
MscI TGG'CCA
Cuts at: 0 468 1674
Size: 468 1206
MslI CAynn'nnrTG
Cuts at: 0 703 799 958 994 1674
Size: 703 96 159 36 680
Fragments arranged by size:
703 680 159 96 36
MspA1I CmG'CkG
Cuts at: 0 353 820 1181 1242 1437 1578 1674
Size: 353 467 361 61 195 141 96
Fragments arranged by size:
467 361 353 195 141 96 61
NarI GG'CG_CC
Cuts at: 0 651 750 1674
Size: 651 99 924
Fragments arranged by size:
924 651 99
NcoI C'CATG_G
Cuts at: 0 989 1674
Size: 989 685
NspI r_CATG'y
Cuts at: 0 169 1674
Size: 169 1505
PasI CC'CwG_GG
Cuts at: 0 828 1674
Size: 828 846
PflMI CCAn_nnn'nTGG
Cuts at: 0 128 1023 1674
Size: 128 895 651
Fragments arranged by size:
895 651 128
PfoI T'CCnGG_A
Cuts at: 0 1124 1674
Size: 1124 550
PmlI CAC'GTG
Cuts at: 0 1085 1674
Size: 1085 589
PpuMI rG'GwC_Cy
Cuts at: 0 1258 1674
Size: 1258 416
PshAI GACnn'nnGTC
Cuts at: 0 1484 1674
Size: 1484 190
PspOMI G'GGCC_C
Cuts at: 0 71 519 1522 1674
Size: 71 448 1003 152
Fragments arranged by size:
1003 448 152 71
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 361 393 1674
Size: 361 32 1281
Fragments arranged by size:
1281 361 32
PstI C_TGCA'G
Cuts at: 0 689 716 1364 1674
Size: 689 27 648 310
Fragments arranged by size:
689 648 310 27
PvuII CAG'CTG
Cuts at: 0 820 1181 1242 1437 1578 1674
Size: 820 361 61 195 141 96
Fragments arranged by size:
820 361 195 141 96 61
SalI G'TCGA_C
Cuts at: 0 472 1674
Size: 472 1202
SexAI A'CCwGG_T
Cuts at: 0 629 1145 1674
Size: 629 516 529
Fragments arranged by size:
629 529 516
SfcI C'TryA_G
Cuts at: 0 685 712 1360 1674
Size: 685 27 648 314
Fragments arranged by size:
685 648 314 27
SfoI GGC'GCC
Cuts at: 0 652 751 1674
Size: 652 99 923
Fragments arranged by size:
923 652 99
SmaI CCC'GGG
Cuts at: 0 1378 1674
Size: 1378 296
SmlI C'TyrA_G
Cuts at: 0 261 512 1674
Size: 261 251 1162
Fragments arranged by size:
1162 261 251
SnaBI TAC'GTA
Cuts at: 0 137 1674
Size: 137 1537
StuI AGG'CCT
Cuts at: 0 1426 1674
Size: 1426 248
StyI C'CwwG_G
Cuts at: 0 24 645 989 1154 1283 1674
Size: 24 621 344 165 129 391
Fragments arranged by size:
621 391 344 165 129 24
TatI w'GTAC_w
Cuts at: 0 245 1263 1674
Size: 245 1018 411
Fragments arranged by size:
1018 411 245
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1228 1260 1293 1325 1674
Size: 1228 32 33 32 349
Fragments arranged by size:
1228 349 33 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 630 996 1020 1674
Size: 630 366 24 654
Fragments arranged by size:
654 630 366 24
XhoI C'TCGA_G
Cuts at: 0 512 1674
Size: 512 1162
XmaI C'CCGG_G
Cuts at: 0 1376 1674
Size: 1376 298
Enzymes that do cut:
AarI AccI AcuI AhdI AleI AlfI AlwNI ApaI
AvaI AvrII BaeI BanI BanII BbeI BbsI BciVI
BglI BlpI Bme1580I BmrI BplI BpmI BpuEI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsmI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI
BstEII BstXI Bsu36I BtgI BtsI EaeI EarI Eco57MI
EcoO109I HaeII Hin4I HincII KasI MmeI MscI MslI
MspA1I NarI NcoI NspI PasI PflMI PfoI PmlI
PpuMI PshAI PspOMI PsrI PstI PvuII SalI SexAI
SfcI SfoI SmaI SmlI SnaBI StuI StyI TatI
TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AloI
ApaLI ApoI AscI AseI AsiSI BamHI BbvCI BcgI
BclI BfrBI BglII BmgBI BmtI Bpu10I BsaI BsaBI
BsiEI BsiHKAI BsiWI BspHI BssHII BssSI BstBI BstYI
BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI NaeI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PpiI PsiI PspXI PvuI RsrII SacI SacII SanDI
SapI SbfI ScaI SfiI SgfI SgrAI SpeI SphI
SrfI SspI SwaI TaqII Tth111I XbaI XmnI ZraI