Restriction map for
pFN21AE1034
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI AlwI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BcgI BclI BfrI BglII
BlnI BpmI BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstYI BstZ17I Cfr10I ClaI
CpoI DraI DraIII Eam1105I Eco47III
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI MamI MfeI MflI MluI
MroI MseI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tru9I
Tsp509I Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 25 enzymes) #
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Alw44I 121 GTGCAC G^TGCAC
ApaLI 121 GTGCAC G^TGCAC
AspHI 121 GTGCAC G[AT]GC[AT]^C
BanII 635 GAGCCC G[GA]GC[CT]^C
BfaI 824 CTAG C^TAG
BlpI 565 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 565 GCTGAGC GC^T[ACGT]AGC
BseRI 711 GAGGAG GAGGAG(10/8)
BsiHKAI 121 GTGCAC G[AT]GC[AT]^C
BsmAI 334 GAGAC GTCTC(1/5)
BspHI 203 TCATGA T^CATGA
CelII 565 GCTGAGC GC^T[ACGT]AGC
DpnII 1029 GATC ^GATC
DrdI 253 GACATCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeII 1184 AGCGCC [GA]GCGC^[CT]
MaeI 824 CTAG C^TAG
MboI 1029 GATC ^GATC
NcoI 758 CCATGG C^CATGG
NdeII 1029 GATC ^GATC
RcaI 203 TCATGA T^CATGA
Sau3AI 1029 GATC ^GATC
SmlI 217 CTCAAG C^T[CT][GA]AG
SspI 487 AATATT AAT^ATT
TaqI 1025 TCGA T^CGA
TthHB8I 1025 TCGA T^CGA
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# TWO CUT ENZYMES ( 21 enzymes) #
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AlwNI 4 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
313 CAGTTTCTG
BalI 389 TGGCCA TGG^CCA
770 TGGCCA
BciVI 597 GGATAC GTATCC(6/5)
740 GTATCC
BsgI 1140 CTGCAC GTGCAG(16/14)
1112 GTGCAG
BsiEI 41 CGGCCG CG[GA][CT]^CG
101 CGGCCG
BsrBI 81 GAGCGG CCGCTC(-3/-3)
568 GAGCGG
BstAPI 3 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
12 GCAGGTAGTGC
BstXI 455 CCATGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
851 CCATCCTGCTGG
EagI 41 CGGCCG C^GGCCG
101 CGGCCG
EarI 880 GAAGAG CTCTTC(1/4)
1194 GAAGAG
EclXI 41 CGGCCG C^GGCCG
101 CGGCCG
Eco52I 41 CGGCCG C^GGCCG
101 CGGCCG
FauI 90 CCCGC CCCGC(4/6)
151 GCGGG
HphI 831 GGTGA GGTGA(8/7)
1095 GGTGA
Ksp632I 880 GAAGAG CTCTTC(1/4)
1194 GAAGAG
MluNI 389 TGGCCA TGG^CCA
770 TGGCCA
MscI 389 TGGCCA TGG^CCA
770 TGGCCA
PleI 1079 GACTC GAGTC(4/5)
451 GAGTC
PpuMI 905 GGGTCCT [GA]G^G[AT]CC[CT]
932 GGGTCCC
PstI 10 CTGCAG CTGCA^G
274 CTGCAG
StuI 95 AGGCCT AGG^CCT
1037 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AluI AocI AvaII
BanI BbvI BcnI BglI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI Bsu36I Cac8I CfoI Cfr13I
DdeI DraII DsaI EaeI Eco81I
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI KspI MaeII MaeIII
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV PvuII SacII Sau96I ScrFI
SfaNI SfcI StyI TfiI TseI
Tsp45I TspRI XcmI