(Circular) (Six-base) MAPSORT of: pFN21AE1034.seq Check: 3622 from: 1 to: 5977 pFN21AE1034 5977 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2396 2396 Size: 5977 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3787 278 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3320 1937 Size: 1383 4594 Acc65I G'GTAC_C Cuts at: 3298 3298 Size: 5977 AclI AA'CG_TT Cuts at: 1563 3276 4104 4477 1563 Size: 1713 828 373 3063 Fragments arranged by size: 3063 1713 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2804 3206 4040 5424 5692 1632 Size: 1172 402 834 1384 268 1917 Fragments arranged by size: 1917 1384 1172 834 402 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5780 AflIII A'CryG_T Cuts at: 1203 4876 5853 1203 Size: 3673 977 1327 Fragments arranged by size: 3673 1327 977 AgeI A'CCGG_T Cuts at: 4808 4808 Size: 5977 AhdI GACnn_n'nnGTC Cuts at: 4706 4706 Size: 5977 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5977 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5977 AlwNI CAG_nnn'CTG Cuts at: 2014 2323 3720 5292 2014 Size: 309 1397 1572 2699 Fragments arranged by size: 2699 1572 1397 309 ApaLI G'TGCA_C Cuts at: 2126 4034 5190 2126 Size: 1908 1156 2913 Fragments arranged by size: 2913 1908 1156 ApoI r'AATT_y Cuts at: 1493 3286 3512 3664 5773 1493 Size: 1793 226 152 2109 1697 Fragments arranged by size: 2109 1793 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4531 160 Size: 872 3499 1606 Fragments arranged by size: 3499 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 AvaI C'yCGr_G Cuts at: 1870 1943 3302 1870 Size: 73 1359 4545 Fragments arranged by size: 4545 1359 73 BamHI G'GATC_C Cuts at: 3307 3674 3307 Size: 367 5610 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2090 2708 3132 3298 4753 Size: 325 217 627 303 618 424 166 1455 Cuts at: 4753 618 Size: 1842 Fragments arranged by size: 1842 1455 627 618 424 325 303 217 166 BanII G_rGCy'C Cuts at: 1391 2644 3296 1391 Size: 1253 652 4072 Fragments arranged by size: 4072 1253 652 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5006 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4168 4202 1903 Size: 34 50 34 2147 34 3678 Fragments arranged by size: 3678 2147 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2596 2756 3869 5079 2596 Size: 160 1113 1210 3494 Fragments arranged by size: 3494 1210 1113 160 BclI T'GATC_A Cuts at: 1548 1548 Size: 5977 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2099 2157 2166 Size: 107 122 71 631 605 427 58 9 Cuts at: 2166 4588 136 Size: 2422 1525 Fragments arranged by size: 2422 1525 631 605 427 122 107 71 58 9 BglII A'GATC_T Cuts at: 5972 5972 Size: 5977 BlpI GC'TnA_GC Cuts at: 2571 3394 2571 Size: 823 5154 Bme1580I G_kGCm'C Cuts at: 1790 2095 2130 2711 3135 3157 4038 5194 1790 Size: 305 35 581 424 22 881 1156 2573 Fragments arranged by size: 2573 1156 881 581 424 305 35 22 BmgBI CAC'GTC Cuts at: 1318 2116 2230 3019 1318 Size: 798 114 789 4276 Fragments arranged by size: 4276 798 789 114 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2311 2975 3149 Size: 416 53 83 399 24 894 664 174 Cuts at: 3149 3159 3708 4666 442 Size: 10 549 958 1753 Fragments arranged by size: 1753 958 894 664 549 416 399 174 83 53 24 10 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5977 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2215 2215 Size: 5977 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4637 1239 Size: 3398 2579 Bpu10I CC'TnA_GC Cuts at: 2084 2084 Size: 5977 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2207 4102 4967 5265 2207 Size: 1895 865 298 2919 Fragments arranged by size: 2919 1895 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4640 882 Size: 617 3141 2219 Fragments arranged by size: 3141 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3768 493 Size: 711 2564 2702 Fragments arranged by size: 2702 2564 711 BsaBI GATnn'nnATC Cuts at: 5971 5971 Size: 5977 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3784 4166 4801 275 Size: 53 83 186 936 2251 382 635 1451 Fragments arranged by size: 2251 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4410 4808 5082 5229 5640 4410 Size: 398 274 147 411 4747 Fragments arranged by size: 4747 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2447 2477 1178 Size: 30 165 30 1044 30 4678 Fragments arranged by size: 4678 1044 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2731 1186 Size: 1545 4432 BseYI C'CCAG_C Cuts at: 2026 2251 2622 2753 5180 5676 2026 Size: 225 371 131 2427 496 2327 Fragments arranged by size: 2427 2327 496 371 225 131 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3130 3138 1163 Size: 573 1394 8 4002 Fragments arranged by size: 4002 1394 573 8 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2049 2109 3427 4188 5216 1540 Size: 275 186 48 60 1318 761 1028 2301 Fragments arranged by size: 2301 1318 1028 761 275 186 60 48 BsiHKAI G_wGCw'C Cuts at: 2130 4038 4123 5194 2130 Size: 1908 85 1071 2913 Fragments arranged by size: 2913 1908 1071 85 BsmI GAATG_Cn' Cuts at: 3488 3581 3488 Size: 93 5884 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2095 2130 2644 2711 3135 3157 3296 Size: 399 305 35 514 67 424 22 139 Cuts at: 3296 4038 4123 5194 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 514 424 399 305 139 85 67 35 22 BspEI T'CCGG_A Cuts at: 5640 5640 Size: 5977 BspHI T'CATG_A Cuts at: 2208 3866 5596 2208 Size: 1658 1730 2589 Fragments arranged by size: 2589 1730 1658 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2008 2396 2974 3332 844 Size: 1164 388 578 358 3489 Fragments arranged by size: 3489 1164 578 388 358 BsrBI CCG'CTC Cuts at: 1394 1823 2088 2575 3864 1394 Size: 429 265 487 1289 3507 Fragments arranged by size: 3507 1289 487 429 265 BsrDI GCAATG_nn' Cuts at: 65 1248 4472 4646 65 Size: 1183 3224 174 1396 Fragments arranged by size: 3224 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4621 4808 1700 Size: 110 2811 187 2869 Fragments arranged by size: 2869 2811 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5977 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5977 BssSI C'ACGA_G Cuts at: 4037 5049 4037 Size: 1012 4965 BstAPI GCAn_nnn'nTGC Cuts at: 794 2014 2023 794 Size: 1220 9 4748 Fragments arranged by size: 4748 1220 9 BstBI TT'CG_AA Cuts at: 4781 4781 Size: 5977 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5977 BstXI CCAn_nnnn'nTGG Cuts at: 2467 2863 2467 Size: 396 5581 BstYI r'GATC_y Cuts at: 1972 3307 3674 4059 4076 5528 5614 5626 5972 Size: 1335 367 385 17 1452 86 12 346 Cuts at: 5972 1972 Size: 1977 Fragments arranged by size: 1977 1452 1335 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2036 2915 3127 2036 Size: 879 212 4886 Fragments arranged by size: 4886 879 212 BtgI C'CryG_G Cuts at: 513 1065 2109 2161 2214 2688 2763 3193 513 Size: 552 1044 52 53 474 75 430 3297 Fragments arranged by size: 3297 1044 552 474 430 75 53 52 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5238 Fragments arranged by size: 5238 683 56 BtsI GCAGTG_nn' Cuts at: 2397 2934 3126 3493 4287 4307 2397 Size: 537 192 367 794 20 4067 Fragments arranged by size: 4067 794 537 367 192 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5977 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5942 DraI TTT'AAA Cuts at: 3281 3281 Size: 5977 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2264 4984 809 Size: 507 948 2720 1802 Fragments arranged by size: 2720 1802 948 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2106 2142 Size: 54 1425 50 275 18 216 60 36 Cuts at: 2142 2394 2775 3424 4313 5942 8 Size: 252 381 649 889 1629 43 Fragments arranged by size: 1629 1425 889 649 381 275 252 216 60 54 50 43 36 18 EagI C'GGCC_G Cuts at: 1537 1812 2046 2106 3424 1537 Size: 275 234 60 1318 4090 Fragments arranged by size: 4090 1318 275 234 60 EarI CTCTTCn'nnn_ Cuts at: 1353 2880 3194 3907 1353 Size: 1527 314 713 3423 Fragments arranged by size: 3423 1527 713 314 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2738 4564 4938 5084 387 Size: 1064 138 1149 1826 374 146 1280 Fragments arranged by size: 1826 1280 1149 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2804 3206 4040 4637 5424 5692 1239 Size: 393 1172 402 834 597 787 268 1524 Fragments arranged by size: 1524 1172 834 787 597 402 393 268 EcoO109I rG'GnC_Cy Cuts at: 2770 2911 2938 2770 Size: 141 27 5809 Fragments arranged by size: 5809 141 27 EcoRI G'AATT_C Cuts at: 3286 3664 3286 Size: 378 5599 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2809 2809 Size: 5977 FspI TGC'GCA Cuts at: 4483 4483 Size: 5977 HaeII r_GCGC'y Cuts at: 1678 3193 5124 1678 Size: 1515 1931 2531 Fragments arranged by size: 2531 1931 1515 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2447 2479 3150 3182 4618 Size: 32 135 32 875 32 671 32 1436 Cuts at: 4618 4650 4692 4724 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1436 875 671 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3321 3567 669 Size: 988 281 1383 246 3079 Fragments arranged by size: 3079 1383 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5977 HpaI GTT'AAC Cuts at: 3567 3567 Size: 5977 KpnI G_GTAC'C Cuts at: 3302 3302 Size: 5977 MfeI C'AATT_G Cuts at: 3576 3576 Size: 5977 MluI A'CGCG_T Cuts at: 5853 5853 Size: 5977 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2248 5091 5275 1305 Size: 943 2843 184 2007 Fragments arranged by size: 2843 2007 943 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2396 2777 10 Size: 54 1425 343 564 381 3210 Fragments arranged by size: 3210 1425 564 381 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2438 2726 2762 3935 4294 4453 518 Size: 648 1272 288 36 1173 359 159 2042 Fragments arranged by size: 2042 1272 1173 648 359 288 159 36 MspA1I CmG'CkG Cuts at: 1606 2111 2163 2216 2251 2819 2852 3251 3393 Size: 505 52 53 35 568 33 399 142 Cuts at: 3393 4070 5218 5463 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 568 505 399 245 142 53 52 35 33 NcoI C'CATG_G Cuts at: 513 1065 2763 513 Size: 552 1698 3727 Fragments arranged by size: 3727 1698 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5977 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5977 NotI GC'GGCC_GC Cuts at: 3424 3424 Size: 5977 NspI r_CATG'y Cuts at: 1132 1229 3335 4880 1132 Size: 97 2106 1545 2229 Fragments arranged by size: 2229 2106 1545 97 PasI CC'CwG_GG Cuts at: 1348 3109 3269 1348 Size: 1761 160 4056 Fragments arranged by size: 4056 1761 160 PciI A'CATG_T Cuts at: 4876 4876 Size: 5977 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5977 PfoI T'CCnGG_A Cuts at: 2334 2334 Size: 5977 PmeI GTTT'AAAC Cuts at: 3281 3281 Size: 5977 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4046 4078 5559 5591 4046 Size: 32 1481 32 4432 Fragments arranged by size: 4432 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2911 2938 2911 Size: 27 5950 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5977 PsiI TTA'TAA Cuts at: 3547 3547 Size: 5977 PstI C_TGCA'G Cuts at: 830 2019 2283 3329 830 Size: 1189 264 1046 3478 Fragments arranged by size: 3478 1189 1046 264 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5977 PvuII CAG'CTG Cuts at: 2251 2819 2852 2251 Size: 568 33 5376 Fragments arranged by size: 5376 568 33 SacII CC_GC'GG Cuts at: 2112 2164 2217 2112 Size: 52 53 5872 Fragments arranged by size: 5872 53 52 SalI G'TCGA_C Cuts at: 1936 3319 1936 Size: 1383 4594 SanDI GG'GwC_CC Cuts at: 2938 2938 Size: 5977 SbfI CC_TGCA'GG Cuts at: 3329 3329 Size: 5977 ScaI AGT'ACT Cuts at: 4225 5937 4225 Size: 1712 4265 SfcI C'TryA_G Cuts at: 826 1045 2015 2279 2384 3325 4460 5141 5332 Size: 219 970 264 105 941 1135 681 191 Cuts at: 5332 826 Size: 1471 Fragments arranged by size: 1471 1135 970 941 681 264 219 191 105 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5977 SmaI CCC'GGG Cuts at: 1872 3304 1872 Size: 1432 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2222 4081 4982 5244 820 Size: 197 926 279 1859 901 262 1553 Fragments arranged by size: 1859 1553 926 901 279 262 197 SnaBI TAC'GTA Cuts at: 493 3768 493 Size: 3275 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5977 SphI G_CATG'C Cuts at: 1132 3335 1132 Size: 2203 3774 SspI AAT'ATT Cuts at: 5 52 2494 3901 5 Size: 47 2442 1407 2081 Fragments arranged by size: 2442 2081 1407 47 StuI AGG'CCT Cuts at: 1517 2102 3044 1517 Size: 585 942 4450 Fragments arranged by size: 4450 942 585 StyI C'CwwG_G Cuts at: 513 1065 2763 2877 2905 3416 513 Size: 552 1698 114 28 511 3074 Fragments arranged by size: 3074 1698 552 511 114 28 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3150 4004 4021 4174 4359 4804 325 Size: 1255 1570 854 17 153 185 445 1498 Fragments arranged by size: 1570 1498 1255 854 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4223 5935 96 Size: 274 80 33 51 1443 2246 1712 138 Fragments arranged by size: 2246 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5937 5969 5937 Size: 32 5945 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5977 XbaI T'CTAG_A Cuts at: 3313 3313 Size: 5977 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2761 2770 2840 2761 Size: 9 70 5898 Fragments arranged by size: 5898 70 9 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5977 XmaI C'CCGG_G Cuts at: 1870 3302 1870 Size: 1432 4545 XmnI GAAnn'nnTTC Cuts at: 4106 4106 Size: 5977 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3785 276 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI