Restriction map for
pFN21AE1034
(Linear) (Six-base) MAPSORT of: KIEE1034.seq Check: 5664 from: 1 to: 1272
KIEE1034.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 391 1272
Size: 391 881
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 1201 1272
Size: 799 402 71
Fragments arranged by size:
799 402 71
AlwNI CAG_nnn'CTG
Cuts at: 0 9 318 1272
Size: 9 309 954
Fragments arranged by size:
954 309 9
ApaLI G'TGCA_C
Cuts at: 0 121 1272
Size: 121 1151
BanI G'GyrC_C
Cuts at: 0 85 703 1127 1272
Size: 85 618 424 145
Fragments arranged by size:
618 424 145 85
BanII G_rGCy'C
Cuts at: 0 639 1272
Size: 639 633
BciVI GTATCCnnnnn_n'
Cuts at: 0 591 751 1272
Size: 591 160 521
Fragments arranged by size:
591 521 160
BglI GCCn_nnn'nGGC
Cuts at: 0 94 152 161 1272
Size: 94 58 9 1111
Fragments arranged by size:
1111 94 58 9
BlpI GC'TnA_GC
Cuts at: 0 566 1272
Size: 566 706
Bme1580I G_kGCm'C
Cuts at: 0 90 125 706 1130 1152 1272
Size: 90 35 581 424 22 120
Fragments arranged by size:
581 424 120 90 35 22
BmgBI CAC'GTC
Cuts at: 0 111 225 1014 1272
Size: 111 114 789 258
Fragments arranged by size:
789 258 114 111
BmrI ACTGGGnnnn_n'
Cuts at: 0 306 970 1144 1154 1272
Size: 306 664 174 10 118
Fragments arranged by size:
664 306 174 118 10
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 210 1272
Size: 210 1062
Bpu10I CC'TnA_GC
Cuts at: 0 79 1272
Size: 79 1193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 1272
Size: 202 1070
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 442 472 1272
Size: 442 30 800
Fragments arranged by size:
800 442 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 726 1272
Size: 726 546
BseYI C'CCAG_C
Cuts at: 0 21 246 617 748 1272
Size: 21 225 371 131 524
Fragments arranged by size:
524 371 225 131 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1125 1133 1272
Size: 1125 8 139
Fragments arranged by size:
1125 139 8
BsiEI CG_ry'CG
Cuts at: 0 44 104 1272
Size: 44 60 1168
Fragments arranged by size:
1168 60 44
BsiHKAI G_wGCw'C
Cuts at: 0 125 1272
Size: 125 1147
Bsp1286I G_dGCh'C
Cuts at: 0 90 125 639 706 1130 1152 1272
Size: 90 35 514 67 424 22 120
Fragments arranged by size:
514 424 120 90 67 35 22
BspHI T'CATG_A
Cuts at: 0 203 1272
Size: 203 1069
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3 391 969 1272
Size: 3 388 578 303
Fragments arranged by size:
578 388 303 3
BsrBI CCG'CTC
Cuts at: 0 83 570 1272
Size: 83 487 702
Fragments arranged by size:
702 487 83
BstAPI GCAn_nnn'nTGC
Cuts at: 0 9 18 1272
Size: 9 9 1254
Fragments arranged by size:
1254 9 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 462 858 1272
Size: 462 396 414
Fragments arranged by size:
462 414 396
Bsu36I CC'TnA_GG
Cuts at: 0 31 910 1122 1272
Size: 31 879 212 150
Fragments arranged by size:
879 212 150 31
BtgI C'CryG_G
Cuts at: 0 104 156 209 683 758 1188 1272
Size: 104 52 53 474 75 430 84
Fragments arranged by size:
474 430 104 84 75 53 52
BtsI GCAGTG_nn'
Cuts at: 0 392 929 1121 1272
Size: 392 537 192 151
Fragments arranged by size:
537 392 192 151
DrdI GACnn_nn'nnGTC
Cuts at: 0 259 1272
Size: 259 1013
EaeI y'GGCC_r
Cuts at: 0 41 101 137 389 770 1272
Size: 41 60 36 252 381 502
Fragments arranged by size:
502 381 252 60 41 36
EagI C'GGCC_G
Cuts at: 0 41 101 1272
Size: 41 60 1171
Fragments arranged by size:
1171 60 41
EarI CTCTTCn'nnn_
Cuts at: 0 875 1189 1272
Size: 875 314 83
Fragments arranged by size:
875 314 83
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 733 1272
Size: 733 539
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 1201 1272
Size: 799 402 71
Fragments arranged by size:
799 402 71
EcoO109I rG'GnC_Cy
Cuts at: 0 765 906 933 1272
Size: 765 141 27 339
Fragments arranged by size:
765 339 141 27
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 804 1272
Size: 804 468
HaeII r_GCGC'y
Cuts at: 0 1188 1272
Size: 1188 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 442 474 1145 1177 1272
Size: 442 32 671 32 95
Fragments arranged by size:
671 442 95 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 243 1272
Size: 243 1029
MscI TGG'CCA
Cuts at: 0 391 772 1272
Size: 391 381 500
Fragments arranged by size:
500 391 381
MslI CAynn'nnrTG
Cuts at: 0 433 721 757 1272
Size: 433 288 36 515
Fragments arranged by size:
515 433 288 36
MspA1I CmG'CkG
Cuts at: 0 106 158 211 246 814 847 1246 1272
Size: 106 52 53 35 568 33 399 26
Fragments arranged by size:
568 399 106 53 52 35 33 26
NcoI C'CATG_G
Cuts at: 0 758 1272
Size: 758 514
PasI CC'CwG_GG
Cuts at: 0 1104 1264 1272
Size: 1104 160 8
Fragments arranged by size:
1104 160 8
PfoI T'CCnGG_A
Cuts at: 0 329 1272
Size: 329 943
PpuMI rG'GwC_Cy
Cuts at: 0 906 933 1272
Size: 906 27 339
Fragments arranged by size:
906 339 27
PstI C_TGCA'G
Cuts at: 0 14 278 1272
Size: 14 264 994
Fragments arranged by size:
994 264 14
PvuII CAG'CTG
Cuts at: 0 246 814 847 1272
Size: 246 568 33 425
Fragments arranged by size:
568 425 246 33
SacII CC_GC'GG
Cuts at: 0 107 159 212 1272
Size: 107 52 53 1060
Fragments arranged by size:
1060 107 53 52
SanDI GG'GwC_CC
Cuts at: 0 933 1272
Size: 933 339
SfcI C'TryA_G
Cuts at: 0 10 274 379 1272
Size: 10 264 105 893
Fragments arranged by size:
893 264 105 10
SmlI C'TyrA_G
Cuts at: 0 217 1272
Size: 217 1055
SspI AAT'ATT
Cuts at: 0 489 1272
Size: 489 783
StuI AGG'CCT
Cuts at: 0 97 1039 1272
Size: 97 942 233
Fragments arranged by size:
942 233 97
StyI C'CwwG_G
Cuts at: 0 758 872 900 1272
Size: 758 114 28 372
Fragments arranged by size:
758 372 114 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1145 1272
Size: 1145 127
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 756 765 835 1272
Size: 756 9 70 437
Fragments arranged by size:
756 437 70 9
Enzymes that do cut:
AarI AcuI AlwNI ApaLI BanI BanII BciVI BglI
BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BstAPI BstXI Bsu36I BtgI BtsI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I FalI HaeII Hin4I
MmeI MscI MslI MspA1I NcoI PasI PfoI PpuMI
PstI PvuII SacII SanDI SfcI SmlI SspI StuI
StyI TaqII XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI
BcgI BclI BfrBI BglII BmtI BpmI BsaI BsaAI
BsaBI BsaHI BsaWI BsiWI BsmI BsmBI BspEI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I
BtgZI ClaI CspCI DraI DraIII EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI