################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AgeI AhdI AocI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BamHI BbsI BbvCI BciVI BfaI BfrI BglII BlnI BpuAI BsaBI BsaI BsaWI BseAI BsiWI BsmBI BsmI BspDI BspEI BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I ClaI CpoI DraI Eam1105I Eco81I EcoNI EcoRV EcoT14I EcoT22I FseI HincII HindII HindIII HinfI HpaI KpnI MaeI MamI MfeI MluI MroI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PleI PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SalI SapI SbfI ScaI SexAI SfuI SgrAI SnaBI SpeI SphI Sse8387I StuI StyI SwaI TfiI Tru9I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 66 enzymes) # ################################### AatII 529 GACGTC GACGT^C AcsI 895 GAATTC [GA]^AATT[CT] Alw44I 499 GTGCAC G^TGCAC AlwI 164 GATCC GGATC(4/5) AlwNI 43 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 157 GGGCCC GGGCC^C ApaLI 499 GTGCAC G^TGCAC ApoI 895 GAATTC [GA]^AATT[CT] AspI 987 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC BanII 157 GGGCCC G[GA]GC[CT]^C BbeI 401 GGCGCC GGCGC^C BbrPI 817 CACGTG CAC^GTG BcgI 1005 CGAGGCCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 562 GCCATGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 384 GCTCAGC GC^T[ACGT]AGC BpmI 1054 CTCCAG CTGGAG(16/14) Bpu1102I 384 GCTCAGC GC^T[ACGT]AGC BsaAI 817 CACGTG [CT]AC^GT[GA] BseRI 670 GAGGAG GAGGAG(10/8) BsgI 431 GTGCAG GTGCAG(16/14) BsiEI 681 CGGCCG CG[GA][CT]^CG Bsp1407I 821 TGTACA T^GTACA BspHI 182 TCATGA T^CATGA BspLU11I 317 ACATGT A^CATGT BsrBI 382 CCGCTC CCGCTC(-3/-3) BsrDI 247 CATTGC GCAATG(2/0) BsrGI 821 TGTACA T^GTACA BsrI 263 ACTGG ACTGG(1/-1) BstAPI 42 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 294 GGTGACC G^GT[ACGT]ACC BstPI 294 GGTGACC G^GT[ACGT]ACC BstYI 163 AGATCC [GA]^GATC[CT] CelII 384 GCTCAGC GC^T[ACGT]AGC DrdI 456 GACCGCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 681 CGGCCG C^GGCCG EclXI 681 CGGCCG C^GGCCG Eco52I 681 CGGCCG C^GGCCG EcoO65I 294 GGTGACC G^GT[ACGT]ACC EcoRI 895 GAATTC G^AATTC KasI 401 GGCGCC G^GCGCC KspI 716 CCGCGG CCGC^GG MflI 163 AGATCC [GA]^GATC[CT] NaeI 146 GCCGGC GCC^GGC NarI 401 GGCGCC GG^CGCC NgoMIV 146 GCCGGC G^CCGGC NspI 317 ACATGT [GA]CATG^[CT] PflFI 987 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 392 CCAAGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 817 CACGTG CAC^GTG PmlI 817 CACGTG CAC^GTG PstI 40 CTGCAG CTGCA^G RcaI 182 TCATGA T^CATGA SacII 716 CCGCGG CCGC^GG SfcI 40 CTGCAG C^T[GA][CT]AG SfiI 561 GGCCATGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 401 GGCGCC GGC^GCC SspBI 821 TGTACA T^GTACA SspI 917 AATATT AAT^ATT TaqI 527 TCGA T^CGA Tsp509I 896 AATT ^AATT TspRI 262 CACTG CA[GC]TG[ACGT][ACGT]^ Tth111I 987 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 527 TCGA T^CGA Van91I 392 CCAAGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 941 CCAGAGACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 163 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcyI 401 GGCGCC G[GA]^CG[CT]C 529 GACGTC AflIII 317 ACATGT A^C[GA][CT]GT 818 ACGTGT Aor51HI 359 AGCGCT AGC^GCT 395 AGCGCT AspHI 499 GTGCAC G[AT]GC[AT]^C 766 GTGCTC AvaI 114 CCCGGG C^[CT]CG[GA]G 890 CCCGGG AviII 662 TGCGCA TGC^GCA 1014 TGCGCA BalI 215 TGGCCA TGG^CCA 560 TGGCCA BanI 401 GGCGCC G^G[CT][GA]CC 936 GGTGCC BclI 185 TGATCA T^GATCA 620 TGATCA BsaHI 401 GGCGCC G[GA]^CG[CT]C 529 GACGTC BsiHKAI 499 GTGCAC G[AT]GC[AT]^C 766 GTGCTC BsmAI 944 GAGAC GTCTC(1/5) 839 GTCTC BsoBI 114 CCCGGG C^[CT]CG[GA]G 890 CCCGGG BssHII 475 GCGCGC G^CGCGC 699 GCGCGC DraIII 778 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG 831 CACGCTGTG Eco47III 359 AGCGCT AGC^GCT 395 AGCGCT FbaI 185 TGATCA T^GATCA 620 TGATCA FspI 662 TGCGCA TGC^GCA 1014 TGCGCA Hin1I 401 GGCGCC G[GA]^CG[CT]C 529 GACGTC MluNI 215 TGGCCA TGG^CCA 560 TGGCCA MscI 215 TGGCCA TGG^CCA 560 TGGCCA MslI 177 CATCTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 990 CACGTCCGTG PvuII 843 CAGCTG CAG^CTG 901 CAGCTG SmaI 114 CCCGGG CCC^GGG 890 CCCGGG SmlI 535 CTCAAG C^T[CT][GA]AG 652 CTCAAG XmaCI 114 CCCGGG C^CCGGG 890 CCCGGG XmaI 114 CCCGGG C^CCGGG 890 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AccII AciI AfaI AluI AvaII BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BspMI BsrFI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I