(Circular) (Six-base) MAPSORT of: pFN21AE9575.seq Check: 8987 from: 1 to: 5782 pFN21AE9575 5782 bp With 182 enzymes: SgfI * July 6, 2012 17:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2534 2756 2534 Size: 222 5560 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2538 3592 278 Size: 53 83 186 936 1002 1054 2468 Fragments arranged by size: 2468 1054 1002 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3125 1937 Size: 1188 4594 Acc65I G'GTAC_C Cuts at: 3103 3103 Size: 5782 AclI AA'CG_TT Cuts at: 1563 3909 4282 1563 Size: 2346 373 3063 Fragments arranged by size: 3063 2346 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3048 3845 5229 5497 1632 Size: 1416 797 1384 268 1917 Fragments arranged by size: 1917 1416 1384 797 268 AfeI AGC'GCT Cuts at: 2366 2402 2366 Size: 36 5746 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5585 AflIII A'CryG_T Cuts at: 1203 2322 2823 4681 5658 1203 Size: 1119 501 1858 977 1327 Fragments arranged by size: 1858 1327 1119 977 501 AgeI A'CCGG_T Cuts at: 4613 4613 Size: 5782 AhdI GACnn_n'nnGTC Cuts at: 4511 4511 Size: 5782 AleI CACnn'nnGTG Cuts at: 1166 2999 1166 Size: 1833 3949 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5782 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3062 3094 3062 Size: 32 5750 AlwNI CAG_nnn'CTG Cuts at: 2053 3525 5097 2053 Size: 1472 1572 2738 Fragments arranged by size: 2738 1572 1472 ApaI G_GGCC'C Cuts at: 2166 2166 Size: 5782 ApaLI G'TGCA_C Cuts at: 2504 3839 4995 2504 Size: 1335 1156 3291 Fragments arranged by size: 3291 1335 1156 ApoI r'AATT_y Cuts at: 1493 2900 3091 3317 3469 5578 1493 Size: 1407 191 226 152 2109 1697 Fragments arranged by size: 2109 1697 1407 226 191 152 AseI AT'TA_AT Cuts at: 160 1032 4336 160 Size: 872 3304 1606 Fragments arranged by size: 3304 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 AvaI C'yCGr_G Cuts at: 1870 1943 2119 2895 3107 1870 Size: 73 176 776 212 4545 Fragments arranged by size: 4545 776 212 176 73 BamHI G'GATC_C Cuts at: 3112 3479 3112 Size: 367 5415 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2406 2941 3103 4558 618 Size: 325 217 627 619 535 162 1455 1842 Fragments arranged by size: 1842 1455 627 619 535 325 217 162 BanII G_rGCy'C Cuts at: 1391 2166 3101 1391 Size: 775 935 4072 Fragments arranged by size: 4072 935 775 BbeI G_GCGC'C Cuts at: 2410 2410 Size: 5782 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4811 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2999 3033 3973 4007 1903 Size: 34 50 34 978 34 940 34 3678 Fragments arranged by size: 3678 978 940 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3674 4884 3674 Size: 1210 4572 BclI T'GATC_A Cuts at: 1548 2190 2625 1548 Size: 642 435 4705 Fragments arranged by size: 4705 642 435 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2573 4393 136 Size: 107 122 71 631 605 901 1820 1525 Fragments arranged by size: 1820 1525 901 631 605 122 107 71 BglII A'GATC_T Cuts at: 5777 5777 Size: 5782 BlpI GC'TnA_GC Cuts at: 2390 3199 2390 Size: 809 4973 Bme1580I G_kGCm'C Cuts at: 1790 2166 2508 2946 3843 4999 1790 Size: 376 342 438 897 1156 2573 Fragments arranged by size: 2573 1156 897 438 376 342 BmgBI CAC'GTC Cuts at: 1318 2997 1318 Size: 1679 4103 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2278 3513 4471 Size: 416 53 83 399 24 861 1235 958 Cuts at: 4471 442 Size: 1753 Fragments arranged by size: 1753 1235 958 861 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5782 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3044 4442 1239 Size: 1805 1398 2579 Fragments arranged by size: 2579 1805 1398 Bpu10I CC'TnA_GC Cuts at: 2198 2198 Size: 5782 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2525 2642 3907 4772 5070 2525 Size: 117 1265 865 298 3237 Fragments arranged by size: 3237 1265 865 298 117 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4445 882 Size: 617 2946 2219 Fragments arranged by size: 2946 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2824 3573 493 Size: 711 1620 749 2702 Fragments arranged by size: 2702 1620 749 711 BsaBI GATnn'nnATC Cuts at: 5776 5776 Size: 5782 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2407 2535 3589 3971 Size: 53 83 186 936 874 128 1054 382 Cuts at: 3971 4606 275 Size: 635 1451 Fragments arranged by size: 1451 1054 936 874 635 382 186 128 83 53 BsaWI w'CCGG_w Cuts at: 4215 4613 4887 5034 5445 4215 Size: 398 274 147 411 4552 Fragments arranged by size: 4552 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3062 3092 1178 Size: 30 165 30 1659 30 3868 Fragments arranged by size: 3868 1659 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2690 1186 Size: 1504 4278 BseYI C'CCAG_C Cuts at: 2558 4985 5481 2558 Size: 2427 496 2859 Fragments arranged by size: 2859 2427 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2457 1163 Size: 573 721 4488 Fragments arranged by size: 4488 721 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2689 3232 3993 5021 1540 Size: 275 186 688 543 761 1028 2301 Fragments arranged by size: 2301 1028 761 688 543 275 186 BsiHKAI G_wGCw'C Cuts at: 2508 2775 3843 3928 4999 2508 Size: 267 1068 85 1071 3291 Fragments arranged by size: 3291 1071 1068 267 85 BsmI GAATG_Cn' Cuts at: 3293 3386 3293 Size: 93 5689 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2166 2508 2775 2946 3101 3843 3928 Size: 399 376 342 267 171 155 742 85 Cuts at: 3928 4999 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 376 342 267 171 155 85 BspEI T'CCGG_A Cuts at: 5445 5445 Size: 5782 BspHI T'CATG_A Cuts at: 2187 3671 5401 2187 Size: 1484 1730 2568 Fragments arranged by size: 2568 1730 1484 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 1999 2534 2756 3137 844 Size: 1155 535 222 381 3489 Fragments arranged by size: 3489 1155 535 381 222 BsrBI CCG'CTC Cuts at: 1394 1823 2389 3669 1394 Size: 429 566 1280 3507 Fragments arranged by size: 3507 1280 566 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2251 4277 4451 65 Size: 1183 1003 2026 174 1396 Fragments arranged by size: 2026 1396 1183 1003 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2024 2151 3014 4426 4613 1700 Size: 110 214 127 863 1412 187 2869 Fragments arranged by size: 2869 1412 863 214 187 127 110 BsrGI T'GTAC_A Cuts at: 96 2826 96 Size: 2730 3052 BssHII G'CGCG_C Cuts at: 1925 2480 2704 1925 Size: 555 224 5003 Fragments arranged by size: 5003 555 224 BssSI C'ACGA_G Cuts at: 3842 4854 3842 Size: 1012 4770 BstAPI GCAn_nnn'nTGC Cuts at: 794 2053 794 Size: 1259 4523 BstBI TT'CG_AA Cuts at: 4586 4586 Size: 5782 BstEII G'GTnAC_C Cuts at: 1184 2299 1184 Size: 1115 4667 BstYI r'GATC_y Cuts at: 1972 2168 3112 3479 3864 3881 5333 5419 5431 Size: 196 944 367 385 17 1452 86 12 Cuts at: 5431 5777 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 944 385 367 346 196 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2085 2304 2631 2721 513 Size: 552 1020 219 327 90 3574 Fragments arranged by size: 3574 1020 552 327 219 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5043 Fragments arranged by size: 5043 683 56 BtsI GCAGTG_nn' Cuts at: 3298 4092 4112 3298 Size: 794 20 4968 Fragments arranged by size: 4968 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5782 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5747 DraI TTT'AAA Cuts at: 3086 3086 Size: 5782 DraIII CAC_nnn'GTG Cuts at: 2788 2841 2788 Size: 53 5729 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2467 4789 809 Size: 507 1151 2322 1802 Fragments arranged by size: 2322 1802 1151 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2220 2427 2565 Size: 54 1425 50 275 18 390 207 138 Cuts at: 2565 2646 2686 2718 3229 4118 5747 8 Size: 81 40 32 511 889 1629 43 Fragments arranged by size: 1629 1425 889 511 390 275 207 138 81 54 50 43 40 32 18 EagI C'GGCC_G Cuts at: 1537 1812 2686 3229 1537 Size: 275 874 543 4090 Fragments arranged by size: 4090 874 543 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2135 2591 2984 3712 1353 Size: 782 456 393 728 3423 Fragments arranged by size: 3423 782 728 456 393 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2974 4369 4743 4889 387 Size: 1064 138 1385 1395 374 146 1280 Fragments arranged by size: 1395 1385 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3044 3048 3845 4442 5229 5497 1239 Size: 393 1412 4 797 597 787 268 1524 Fragments arranged by size: 1524 1412 797 787 597 393 268 4 EcoO109I rG'GnC_Cy Cuts at: 2011 2162 2377 2442 2011 Size: 151 215 65 5351 Fragments arranged by size: 5351 215 151 65 EcoRI G'AATT_C Cuts at: 2900 3091 3469 2900 Size: 191 378 5213 Fragments arranged by size: 5213 378 191 FspI TGC'GCA Cuts at: 2669 3021 4288 2669 Size: 352 1267 4163 Fragments arranged by size: 4163 1267 352 FspAI rTGC'GCAy Cuts at: 3021 3021 Size: 5782 HaeII r_GCGC'y Cuts at: 1678 2368 2404 2410 4929 1678 Size: 690 36 6 2519 2531 Fragments arranged by size: 2531 2519 690 36 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4423 4455 4497 4529 1373 Size: 32 135 32 2851 32 42 32 2626 Fragments arranged by size: 2851 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3126 3372 669 Size: 988 281 1188 246 3079 Fragments arranged by size: 3079 1188 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5782 HpaI GTT'AAC Cuts at: 3372 3372 Size: 5782 KasI G'GCGC_C Cuts at: 2406 2406 Size: 5782 KpnI G_GTAC'C Cuts at: 3107 3107 Size: 5782 MfeI C'AATT_G Cuts at: 3381 3381 Size: 5782 MluI A'CGCG_T Cuts at: 5658 5658 Size: 5782 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4896 5080 1305 Size: 3591 184 2007 Fragments arranged by size: 3591 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2222 2567 10 Size: 54 1425 343 390 345 3225 Fragments arranged by size: 3225 1425 390 345 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2186 2999 3740 4099 4258 518 Size: 648 1020 813 741 359 159 2042 Fragments arranged by size: 2042 1020 813 741 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2476 2698 2723 2850 2908 3198 3875 5023 Size: 870 222 25 127 58 290 677 1148 Cuts at: 5023 5268 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 870 677 290 245 222 127 58 25 NaeI GCC'GGC Cuts at: 2153 2153 Size: 5782 NarI GG'CG_CC Cuts at: 2407 2407 Size: 5782 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5230 NdeI CA'TA_TG Cuts at: 387 387 Size: 5782 NgoMIV G'CCGG_C Cuts at: 2151 2151 Size: 5782 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5782 NotI GC'GGCC_GC Cuts at: 3229 3229 Size: 5782 NspI r_CATG'y Cuts at: 1132 1229 2326 3140 4685 1132 Size: 97 1097 814 1545 2229 Fragments arranged by size: 2229 1545 1097 814 97 PasI CC'CwG_GG Cuts at: 1348 2512 1348 Size: 1164 4618 PciI A'CATG_T Cuts at: 2322 4681 2322 Size: 2359 3423 PflMI CCAn_nnn'nTGG Cuts at: 1108 2403 1108 Size: 1295 4487 PmeI GTTT'AAAC Cuts at: 3086 3086 Size: 5782 PmlI CAC'GTG Cuts at: 2824 2824 Size: 5782 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3062 3094 3851 3883 5364 5396 3062 Size: 32 757 32 1481 32 3448 Fragments arranged by size: 3448 1481 757 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2011 2377 2442 2011 Size: 366 65 5351 Fragments arranged by size: 5351 366 65 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5782 PsiI TTA'TAA Cuts at: 3352 3352 Size: 5782 PspOMI G'GGCC_C Cuts at: 2162 2162 Size: 5782 PstI C_TGCA'G Cuts at: 830 2049 3134 830 Size: 1219 1085 3478 Fragments arranged by size: 3478 1219 1085 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5782 PvuII CAG'CTG Cuts at: 2850 2908 2850 Size: 58 5724 SacII CC_GC'GG Cuts at: 2724 2724 Size: 5782 SalI G'TCGA_C Cuts at: 1936 3124 1936 Size: 1188 4594 SbfI CC_TGCA'GG Cuts at: 3134 3134 Size: 5782 ScaI AGT'ACT Cuts at: 4030 5742 4030 Size: 1712 4070 SfcI C'TryA_G Cuts at: 826 1045 2045 3130 4265 4946 5137 826 Size: 219 1000 1085 1135 681 191 1471 Fragments arranged by size: 1471 1135 1085 1000 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2573 2573 Size: 5782 SfoI GGC'GCC Cuts at: 2408 2408 Size: 5782 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5782 SmaI CCC'GGG Cuts at: 1872 2121 2897 3109 1872 Size: 249 776 212 4545 Fragments arranged by size: 4545 776 249 212 SmlI C'TyrA_G Cuts at: 820 1017 1943 2540 2657 3886 4787 5049 820 Size: 197 926 597 117 1229 901 262 1553 Fragments arranged by size: 1553 1229 926 901 597 262 197 117 SnaBI TAC'GTA Cuts at: 493 3573 493 Size: 3080 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5782 SphI G_CATG'C Cuts at: 1132 3140 1132 Size: 2008 3774 SspI AAT'ATT Cuts at: 5 52 2924 3706 5 Size: 47 2872 782 2081 Fragments arranged by size: 2872 2081 782 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5782 StyI C'CwwG_G Cuts at: 513 1065 3221 513 Size: 552 2156 3074 Fragments arranged by size: 3074 2156 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2131 2167 3809 3826 3979 4164 4609 Size: 1255 551 36 1642 17 153 185 445 Cuts at: 4609 325 Size: 1498 Fragments arranged by size: 1642 1498 1255 551 445 185 153 36 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2091 2826 4028 Size: 274 80 33 51 1443 114 735 1202 Cuts at: 4028 5740 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1202 735 274 138 114 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5742 5774 5742 Size: 32 5750 Tth111I GACn'n_nGTC Cuts at: 1316 2995 1316 Size: 1679 4103 XbaI T'CTAG_A Cuts at: 3118 3118 Size: 5782 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2953 2953 Size: 5782 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5782 XmaI C'CCGG_G Cuts at: 1870 2119 2895 3107 1870 Size: 249 776 212 4545 Fragments arranged by size: 4545 776 249 212 XmnI GAAnn'nnTTC Cuts at: 3911 3911 Size: 5782 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2536 3590 276 Size: 53 83 186 936 1002 1054 2468 Fragments arranged by size: 2468 1054 1002 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SanDI SapI SexAI SrfI SwaI