(Linear) (Six-base) MAPSORT of: KIEE9575.seq Check: 651 from: 1 to: 1077 KIEE9575.orf With 182 enzymes: SgfI * July 3, 2012 19:02 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 529 751 1077 Size: 529 222 326 Fragments arranged by size: 529 326 222 AatII G_ACGT'C Cuts at: 0 533 1077 Size: 533 544 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1043 1077 Size: 1043 34 AfeI AGC'GCT Cuts at: 0 361 397 1077 Size: 361 36 680 Fragments arranged by size: 680 361 36 AflIII A'CryG_T Cuts at: 0 317 818 1077 Size: 317 501 259 Fragments arranged by size: 501 317 259 AleI CACnn'nnGTG Cuts at: 0 994 1077 Size: 994 83 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1057 1077 Size: 1057 20 AlwNI CAG_nnn'CTG Cuts at: 0 48 1077 Size: 48 1029 ApaI G_GGCC'C Cuts at: 0 161 1077 Size: 161 916 ApaLI G'TGCA_C Cuts at: 0 499 1077 Size: 499 578 ApoI r'AATT_y Cuts at: 0 895 1077 Size: 895 182 AvaI C'yCGr_G Cuts at: 0 114 890 1077 Size: 114 776 187 Fragments arranged by size: 776 187 114 BanI G'GyrC_C Cuts at: 0 401 936 1077 Size: 401 535 141 Fragments arranged by size: 535 401 141 BanII G_rGCy'C Cuts at: 0 161 1077 Size: 161 916 BbeI G_GCGC'C Cuts at: 0 405 1077 Size: 405 672 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 994 1028 1077 Size: 994 34 49 Fragments arranged by size: 994 49 34 BclI T'GATC_A Cuts at: 0 185 620 1077 Size: 185 435 457 Fragments arranged by size: 457 435 185 BglI GCCn_nnn'nGGC Cuts at: 0 568 1077 Size: 568 509 BlpI GC'TnA_GC Cuts at: 0 385 1077 Size: 385 692 Bme1580I G_kGCm'C Cuts at: 0 161 503 941 1077 Size: 161 342 438 136 Fragments arranged by size: 438 342 161 136 BmgBI CAC'GTC Cuts at: 0 992 1077 Size: 992 85 BmrI ACTGGGnnnn_n' Cuts at: 0 273 1077 Size: 273 804 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1039 1077 Size: 1039 38 Bpu10I CC'TnA_GC Cuts at: 0 193 1077 Size: 193 884 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 520 637 1077 Size: 520 117 440 Fragments arranged by size: 520 440 117 BsaAI yAC'GTr Cuts at: 0 819 1077 Size: 819 258 BsaHI Gr'CG_yC Cuts at: 0 402 530 1077 Size: 402 128 547 Fragments arranged by size: 547 402 128 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1057 1077 Size: 1057 20 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 685 1077 Size: 685 392 BseYI C'CCAG_C Cuts at: 0 553 1077 Size: 553 524 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 452 1077 Size: 452 625 BsiEI CG_ry'CG Cuts at: 0 684 1077 Size: 684 393 BsiHKAI G_wGCw'C Cuts at: 0 503 770 1077 Size: 503 267 307 Fragments arranged by size: 503 307 267 Bsp1286I G_dGCh'C Cuts at: 0 161 503 770 941 1077 Size: 161 342 267 171 136 Fragments arranged by size: 342 267 171 161 136 BspHI T'CATG_A Cuts at: 0 182 1077 Size: 182 895 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 529 751 1077 Size: 529 222 326 Fragments arranged by size: 529 326 222 BsrBI CCG'CTC Cuts at: 0 384 1077 Size: 384 693 BsrDI GCAATG_nn' Cuts at: 0 246 1077 Size: 246 831 BsrFI r'CCGG_y Cuts at: 0 19 146 1009 1077 Size: 19 127 863 68 Fragments arranged by size: 863 127 68 19 BsrGI T'GTAC_A Cuts at: 0 821 1077 Size: 821 256 BssHII G'CGCG_C Cuts at: 0 475 699 1077 Size: 475 224 378 Fragments arranged by size: 475 378 224 BstAPI GCAn_nnn'nTGC Cuts at: 0 48 1077 Size: 48 1029 BstEII G'GTnAC_C Cuts at: 0 294 1077 Size: 294 783 BstYI r'GATC_y Cuts at: 0 163 1077 Size: 163 914 BtgI C'CryG_G Cuts at: 0 80 299 626 716 1077 Size: 80 219 327 90 361 Fragments arranged by size: 361 327 219 90 80 DraIII CAC_nnn'GTG Cuts at: 0 783 836 1077 Size: 783 53 241 Fragments arranged by size: 783 241 53 DrdI GACnn_nn'nnGTC Cuts at: 0 462 1077 Size: 462 615 EaeI y'GGCC_r Cuts at: 0 215 422 560 641 681 713 1077 Size: 215 207 138 81 40 32 364 Fragments arranged by size: 364 215 207 138 81 40 32 EagI C'GGCC_G Cuts at: 0 681 1077 Size: 681 396 EarI CTCTTCn'nnn_ Cuts at: 0 130 586 979 1077 Size: 130 456 393 98 Fragments arranged by size: 456 393 130 98 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 969 1077 Size: 969 108 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1039 1043 1077 Size: 1039 4 34 Fragments arranged by size: 1039 34 4 EcoO109I rG'GnC_Cy Cuts at: 0 6 157 372 437 1077 Size: 6 151 215 65 640 Fragments arranged by size: 640 215 151 65 6 EcoRI G'AATT_C Cuts at: 0 895 1077 Size: 895 182 FspI TGC'GCA Cuts at: 0 664 1016 1077 Size: 664 352 61 Fragments arranged by size: 664 352 61 FspAI rTGC'GCAy Cuts at: 0 1016 1077 Size: 1016 61 HaeII r_GCGC'y Cuts at: 0 363 399 405 1077 Size: 363 36 6 672 Fragments arranged by size: 672 363 36 6 KasI G'GCGC_C Cuts at: 0 401 1077 Size: 401 676 MscI TGG'CCA Cuts at: 0 217 562 1077 Size: 217 345 515 Fragments arranged by size: 515 345 217 MslI CAynn'nnrTG Cuts at: 0 181 994 1077 Size: 181 813 83 Fragments arranged by size: 813 181 83 MspA1I CmG'CkG Cuts at: 0 471 693 718 845 903 1077 Size: 471 222 25 127 58 174 Fragments arranged by size: 471 222 174 127 58 25 NaeI GCC'GGC Cuts at: 0 148 1077 Size: 148 929 NarI GG'CG_CC Cuts at: 0 402 1077 Size: 402 675 NgoMIV G'CCGG_C Cuts at: 0 146 1077 Size: 146 931 NspI r_CATG'y Cuts at: 0 321 1077 Size: 321 756 PasI CC'CwG_GG Cuts at: 0 507 1077 Size: 507 570 PciI A'CATG_T Cuts at: 0 317 1077 Size: 317 760 PflMI CCAn_nnn'nTGG Cuts at: 0 398 1077 Size: 398 679 PmlI CAC'GTG Cuts at: 0 819 1077 Size: 819 258 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1057 1077 Size: 1057 20 PpuMI rG'GwC_Cy Cuts at: 0 6 372 437 1077 Size: 6 366 65 640 Fragments arranged by size: 640 366 65 6 PspOMI G'GGCC_C Cuts at: 0 157 1077 Size: 157 920 PstI C_TGCA'G Cuts at: 0 44 1077 Size: 44 1033 PvuII CAG'CTG Cuts at: 0 845 903 1077 Size: 845 58 174 Fragments arranged by size: 845 174 58 SacII CC_GC'GG Cuts at: 0 719 1077 Size: 719 358 SfcI C'TryA_G Cuts at: 0 40 1077 Size: 40 1037 SfiI GGCCn_nnn'nGGCC Cuts at: 0 568 1077 Size: 568 509 SfoI GGC'GCC Cuts at: 0 403 1077 Size: 403 674 SmaI CCC'GGG Cuts at: 0 116 892 1077 Size: 116 776 185 Fragments arranged by size: 776 185 116 SmlI C'TyrA_G Cuts at: 0 535 652 1077 Size: 535 117 425 Fragments arranged by size: 535 425 117 SspI AAT'ATT Cuts at: 0 919 1077 Size: 919 158 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 126 162 1077 Size: 126 36 915 Fragments arranged by size: 915 126 36 TatI w'GTAC_w Cuts at: 0 86 821 1077 Size: 86 735 256 Fragments arranged by size: 735 256 86 Tth111I GACn'n_nGTC Cuts at: 0 990 1077 Size: 990 87 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 948 1077 Size: 948 129 XmaI C'CCGG_G Cuts at: 0 114 890 1077 Size: 114 776 187 Fragments arranged by size: 776 187 114 ZraI GAC'GTC Cuts at: 0 531 1077 Size: 531 546 Enzymes that do cut: AarI AatII AcuI AfeI AflIII AleI AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BcgI BclI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstEII BstYI BtgI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII KasI MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI PmlI PpiI PpuMI PspOMI PstI PvuII SacII SfcI SfiI SfoI SmaI SmlI SspI TaqII TatI Tth111I XcmI XmaI ZraI Enzymes that do not cut: AccI Acc65I AclI AflII AgeI AhdI AlfI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI BfrBI BglII BmtI BplI BsaI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BssSI BstBI BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI EcoICRI EcoNI EcoRV FalI FseI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI NcoI NdeI NheI NotI NruI NsiI PacI PfoI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI SphI SrfI StuI StyI SwaI TstI XbaI XhoI XmnI