################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## Acc65I AccI AclI AflII Aor51HI AscI AseI AsnI Asp718I AspI AvrII BbvCI BciVI BclI BfrI BglII BlnI BsiWI BsmI Bsp1407I BspDI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII EagI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HpaI KpnI MfeI MluI MseI MunI NaeI NgoMIV NheI NotI NsiI NspV PacI PflFI PmeI PshBI Psp1406I PvuI RsrII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tru9I Tth111I XbaI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AatII 1701 GACGTC GACGT^C AccIII 248 TCCGGA T^CCGGA AflIII 311 ACGTGT A^C[GA][CT]GT AgeI 1322 ACCGGT A^CCGGT BbrPI 1222 CACGTG CAC^GTG BfaI 2053 CTAG C^TAG BlpI 1270 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1270 GCTGAGC GC^T[ACGT]AGC BsaAI 1222 CACGTG [CT]AC^GT[GA] BsaBI 2241 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 248 TCCGGA T^CCGGA BspEI 248 TCCGGA T^CCGGA BstXI 1493 CCAAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1270 GCTGAGC GC^T[ACGT]AGC HindIII 1816 AAGCTT A^AGCTT MaeI 2053 CTAG C^TAG MamI 2241 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 248 TCCGGA T^CCGGA NcoI 1862 CCATGG C^CATGG NdeI 281 CATATG CA^TATG NruI 1755 TCGCGA TCG^CGA PinAI 1322 ACCGGT A^CCGGT PmaCI 1222 CACGTG CAC^GTG PmlI 1222 CACGTG CAC^GTG PshAI 82 GACGCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 2056 GAGCTC GAGCT^C ScaI 344 AGTACT AGT^ACT SmaI 135 CCCGGG CCC^GGG SphI 1958 GCATGC GCATG^C StuI 2006 AGGCCT AGG^CCT XmaCI 135 CCCGGG C^CCGGG XmaI 135 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 40 enzymes) # ################################### AcsI 447 AAATTT [GA]^AATT[CT] 1420 AAATTC AhdI 40 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1746 GACGCAGTGTC Alw44I 412 GTGCAC G^TGCAC 1030 GTGCAC AocI 826 CCTGAGG CC^T[ACGT]AGG 1294 CCTGAGG ApaI 218 GGGCCC GGGCC^C 997 GGGCCC ApaLI 412 GTGCAC G^TGCAC 1030 GTGCAC ApoI 447 AAATTT [GA]^AATT[CT] 1420 AAATTC Asp700I 567 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2403 GAACGACTTC AspEI 40 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1746 GACGCAGTGTC BalI 611 TGGCCA TGG^CCA 1502 TGGCCA BbeI 105 GGCGCC GGCGC^C 1312 GGCGCC BcgI 382 CGAAGAAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1832 GCATCCGCATCG BglI 539 GCCAGGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1653 GCCCCAGCGGC BsaWI 248 TCCGGA [AT]^CCGG[AT] 1322 ACCGGT BsiEI 1320 CGACCG CG[GA][CT]^CG 2190 CGACCG BsmBI 2459 GAGACG CGTCTC(1/5) 2776 GAGACG BspHI 878 TCATGA T^CATGA 2654 TCATGA BssSI 1393 CACGAG CACGAG(-5/-1) 624 CTCGTG Bsu36I 826 CCTGAGG CC^T[ACGT]AGG 1294 CCTGAGG DrdI 306 GACCAACGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1369 GACGCCCACGTC Eam1105I 40 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1746 GACGCAGTGTC Eco81I 826 CCTGAGG CC^T[ACGT]AGG 1294 CCTGAGG EcoNI 797 CCTTCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 984 CCTGAGCCAGG KasI 105 GGCGCC G^GCGCC 1312 GGCGCC KspI 7 CCGCGG CCGC^GG 109 CCGCGG MluNI 611 TGGCCA TGG^CCA 1502 TGGCCA MscI 611 TGGCCA TGG^CCA 1502 TGGCCA NarI 105 GGCGCC GG^CGCC 1312 GGCGCC NspI 1958 GCATGC [GA]CATG^[CT] 2122 ACATGC PaeR7I 1400 CTCGAG C^TCGAG 1678 CTCGAG PflMI 186 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1613 CCATGCCCTGG RcaI 878 TCATGA T^CATGA 2654 TCATGA SacII 7 CCGCGG CCGC^GG 109 CCGCGG SapI 2377 GAAGAGC GCTCTTC(1/4) 147 GCTCTTC SfoI 105 GGCGCC GGC^GCC 1312 GGCGCC TfiI 475 GAATC G^A[AT]TC 572 GATTC Tsp509I 448 AATT ^AATT 1421 AATT Van91I 186 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1613 CCATGCCCTGG XhoI 1400 CTCGAG C^TCGAG 1678 CTCGAG XmnI 567 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2403 GAACGACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaI AvaII AviII BamHI BanI BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TseI Tsp45I TspRI TthHB8I XcmI XhoII