(Circular) (Six-base) MAPSORT of: pFN21AE3507.seq Check: 5782 from: 1 to: 7492 pFN21AE3507 7492 bp With 182 enzymes: SgfI * July 6, 2012 17:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3715 3790 3715 Size: 75 7417 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3710 5302 278 Size: 53 83 186 936 2174 1592 2468 Fragments arranged by size: 2468 2174 1592 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4835 1937 Size: 2898 4594 Acc65I G'GTAC_C Cuts at: 4813 4813 Size: 7492 AclI AA'CG_TT Cuts at: 1563 5619 5992 1563 Size: 4056 373 3063 Fragments arranged by size: 4056 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3134 3343 5555 6939 7207 1632 Size: 1502 209 2212 1384 268 1917 Fragments arranged by size: 2212 1917 1502 1384 268 209 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7295 AflIII A'CryG_T Cuts at: 1203 2316 6391 7368 1203 Size: 1113 4075 977 1327 Fragments arranged by size: 4075 1327 1113 977 AgeI A'CCGG_T Cuts at: 3327 6323 3327 Size: 2996 4496 AhdI GACnn_n'nnGTC Cuts at: 2050 3756 6221 2050 Size: 1706 2465 3321 Fragments arranged by size: 3321 2465 1706 AleI CACnn'nnGTG Cuts at: 1166 3869 4034 1166 Size: 2703 165 4624 Fragments arranged by size: 4624 2703 165 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3866 4377 4502 1184 Size: 2682 511 125 4174 Fragments arranged by size: 4174 2682 511 125 AlwNI CAG_nnn'CTG Cuts at: 2197 3299 3565 3606 4138 4432 5235 6807 2197 Size: 1102 266 41 532 294 803 1572 2882 Fragments arranged by size: 2882 1572 1102 803 532 294 266 41 ApaI G_GGCC'C Cuts at: 2227 3006 2227 Size: 779 6713 ApaLI G'TGCA_C Cuts at: 2417 3035 5549 6705 2417 Size: 618 2514 1156 3204 Fragments arranged by size: 3204 2514 1156 618 ApoI r'AATT_y Cuts at: 1493 2452 3425 4801 5027 5179 7288 1493 Size: 959 973 1376 226 152 2109 1697 Fragments arranged by size: 2109 1697 1376 973 959 226 152 AseI AT'TA_AT Cuts at: 160 1032 6046 160 Size: 872 5014 1606 Fragments arranged by size: 5014 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7492 AvaI C'yCGr_G Cuts at: 1870 1943 2027 2096 2140 3287 3405 3683 4817 Size: 73 84 69 44 1147 118 278 1134 Cuts at: 4817 1870 Size: 4545 Fragments arranged by size: 4545 1147 1134 278 118 84 73 69 44 BamHI G'GATC_C Cuts at: 2739 3048 3084 4822 5189 2739 Size: 309 36 1738 367 5042 Fragments arranged by size: 5042 1738 367 309 36 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2110 2520 3317 3614 3925 Size: 325 217 627 323 410 797 297 311 Cuts at: 3925 4813 6268 618 Size: 888 1455 1842 Fragments arranged by size: 1842 1455 888 797 627 410 325 323 311 297 217 BanII G_rGCy'C Cuts at: 1391 2227 3006 3282 3660 3816 4065 4101 4204 Size: 836 779 276 378 156 249 36 103 Cuts at: 4204 4811 1391 Size: 607 4072 Fragments arranged by size: 4072 836 779 607 378 276 249 156 103 36 BbeI G_GCGC'C Cuts at: 2114 3321 2114 Size: 1207 6285 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2974 3337 4670 928 Size: 971 1075 363 1333 3750 Fragments arranged by size: 3750 1333 1075 971 363 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2376 2410 3826 3860 5683 5717 1903 Size: 34 439 34 1416 34 1823 34 3678 Fragments arranged by size: 3678 1823 1416 439 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5384 6594 5384 Size: 1210 6282 BclI T'GATC_A Cuts at: 1548 1548 Size: 7492 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2550 3664 6103 Size: 107 122 71 631 605 878 1114 2439 Cuts at: 6103 136 Size: 1525 Fragments arranged by size: 2439 1525 1114 878 631 605 122 107 71 BglII A'GATC_T Cuts at: 7487 7487 Size: 7492 BlpI GC'TnA_GC Cuts at: 3276 4909 3276 Size: 1633 5859 Bme1580I G_kGCm'C Cuts at: 1790 2163 2227 2421 3006 3039 3617 4031 4075 Size: 373 64 194 585 33 578 414 44 Cuts at: 4075 5553 6709 1790 Size: 1478 1156 2573 Fragments arranged by size: 2573 1478 1156 585 578 414 373 194 64 44 33 BmgBI CAC'GTC Cuts at: 1318 3382 1318 Size: 2064 5428 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2536 3190 5223 Size: 416 53 83 399 24 1119 654 2033 Cuts at: 5223 6181 442 Size: 958 1753 Fragments arranged by size: 2033 1753 1119 958 654 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7492 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2713 2713 Size: 7492 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2174 2290 2462 2473 2866 3038 3410 4073 Size: 935 116 172 11 393 172 372 663 Cuts at: 4073 4131 4409 4757 6152 1239 Size: 58 278 348 1395 2579 Fragments arranged by size: 2579 1395 935 663 393 372 348 278 172 172 116 58 11 Bpu10I CC'TnA_GC Cuts at: 2990 4263 4710 2990 Size: 1273 447 5772 Fragments arranged by size: 5772 1273 447 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3989 4359 5617 6482 6780 3989 Size: 370 1258 865 298 4701 Fragments arranged by size: 4701 1258 865 370 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2303 2842 4577 6155 882 Size: 617 804 539 1735 1578 2219 Fragments arranged by size: 2219 1735 1578 804 617 539 BsaAI yAC'GTr Cuts at: 493 1204 3229 5283 493 Size: 711 2025 2054 2702 Fragments arranged by size: 2702 2054 2025 711 BsaBI GATnn'nnATC Cuts at: 4250 7486 4250 Size: 3236 4256 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2088 2111 2542 3318 Size: 53 83 186 936 555 23 431 776 Cuts at: 3318 3375 3707 4164 5299 5681 6316 275 Size: 57 332 457 1135 382 635 1451 Fragments arranged by size: 1451 1135 936 776 635 555 457 431 382 332 186 83 57 53 23 BsaWI w'CCGG_w Cuts at: 2253 3327 5925 6323 6597 6744 7155 2253 Size: 1074 2598 398 274 147 411 2590 Fragments arranged by size: 2598 2590 1074 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7267 Fragments arranged by size: 7267 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2702 2849 3248 1186 Size: 1516 147 399 5430 Fragments arranged by size: 5430 1516 399 147 BseYI C'CCAG_C Cuts at: 2701 3261 3660 4024 4270 4442 6695 7191 2701 Size: 560 399 364 246 172 2253 496 3002 Fragments arranged by size: 3002 2253 560 496 399 364 246 172 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2251 3818 3890 3893 3968 4350 4418 Size: 573 515 1567 72 3 75 382 68 Cuts at: 4418 4472 1163 Size: 54 4183 Fragments arranged by size: 4183 1567 573 515 382 75 72 68 54 3 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3328 4198 4942 5703 6731 1540 Size: 275 186 1327 870 744 761 1028 2301 Fragments arranged by size: 2301 1327 1028 870 761 744 275 186 BsiHKAI G_wGCw'C Cuts at: 2298 2421 2699 3039 3645 3774 4065 4212 5553 Size: 123 278 340 606 129 291 147 1341 Cuts at: 5553 5638 6709 2298 Size: 85 1071 3081 Fragments arranged by size: 3081 1341 1071 606 340 291 278 147 129 123 85 BsmI GAATG_Cn' Cuts at: 5003 5096 5003 Size: 93 7399 BsmBI CGTCTCn'nnnn_ Cuts at: 4458 4775 4458 Size: 317 7175 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2163 2227 2298 2421 2699 3006 3039 Size: 399 373 64 71 123 278 307 33 Cuts at: 3039 3282 3617 3645 3660 3774 3816 4031 4065 Size: 243 335 28 15 114 42 215 34 Cuts at: 4065 4075 4101 4204 4212 4811 5553 5638 6709 Size: 10 26 103 8 599 742 85 1071 Cuts at: 6709 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 599 399 373 335 307 278 243 215 123 114 103 85 71 64 42 34 33 28 26 15 10 8 BspEI T'CCGG_A Cuts at: 2253 7155 2253 Size: 4902 2590 BspHI T'CATG_A Cuts at: 2883 4659 5381 7111 2883 Size: 1776 722 1730 3264 Fragments arranged by size: 3264 1776 1730 722 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3051 3715 3790 4539 4847 844 Size: 2207 664 75 749 308 3489 Fragments arranged by size: 3489 2207 749 664 308 75 BsrBI CCG'CTC Cuts at: 1394 1823 2245 2881 3745 5379 1394 Size: 429 422 636 864 1634 3507 Fragments arranged by size: 3507 1634 864 636 429 422 BsrDI GCAATG_nn' Cuts at: 65 1248 5987 6161 65 Size: 1183 4739 174 1396 Fragments arranged by size: 4739 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2090 3327 3922 4743 6136 6323 1700 Size: 110 280 1237 595 821 1393 187 2869 Fragments arranged by size: 2869 1393 1237 821 595 280 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7492 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7492 BssSI C'ACGA_G Cuts at: 2629 3398 5552 6564 2629 Size: 769 2154 1012 3557 Fragments arranged by size: 3557 2154 1012 769 BstAPI GCAn_nnn'nTGC Cuts at: 794 3043 3925 4626 794 Size: 2249 882 701 3660 Fragments arranged by size: 3660 2249 882 701 BstBI TT'CG_AA Cuts at: 6296 6296 Size: 7492 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7492 BstXI CCAn_nnnn'nTGG Cuts at: 3505 3505 Size: 7492 BstYI r'GATC_y Cuts at: 1972 2250 2739 3048 3084 3216 3555 4554 4822 Size: 278 489 309 36 132 339 999 268 Cuts at: 4822 5189 5574 5591 7043 7129 7141 7487 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 999 489 385 367 346 339 309 278 268 132 86 36 17 12 Bsu36I CC'TnA_GG Cuts at: 2832 3300 2832 Size: 468 7024 BtgI C'CryG_G Cuts at: 513 1065 2004 2012 2114 2718 3867 513 Size: 552 939 8 102 604 1149 4138 Fragments arranged by size: 4138 1149 939 604 552 102 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3829 487 Size: 683 56 2603 4150 Fragments arranged by size: 4150 2603 683 56 BtsI GCAGTG_nn' Cuts at: 3761 4170 4349 5008 5802 5822 3761 Size: 409 179 659 794 20 5431 Fragments arranged by size: 5431 794 659 409 179 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7492 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3166 3201 529 Size: 35 2602 35 4820 Fragments arranged by size: 4820 2602 35 35 DraI TTT'AAA Cuts at: 4796 4796 Size: 7492 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2317 3380 6499 809 Size: 507 1001 1063 3119 1802 Fragments arranged by size: 3119 1802 1063 1001 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2616 3507 3714 Size: 54 1425 50 275 18 786 891 207 Cuts at: 3714 4725 4939 5828 7457 8 Size: 1011 214 889 1629 43 Fragments arranged by size: 1629 1425 1011 891 889 786 275 214 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4939 1537 Size: 275 3127 4090 Fragments arranged by size: 4090 3127 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2159 2786 4377 5422 1353 Size: 806 627 1591 1045 3423 Fragments arranged by size: 3423 1591 1045 806 627 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2050 2199 3747 3904 6079 6453 Size: 1064 138 461 149 1548 157 2175 374 Cuts at: 6453 6599 387 Size: 146 1280 Fragments arranged by size: 2175 1548 1280 1064 461 374 157 149 146 138 EcoICRI GAG'CTC Cuts at: 4063 4063 Size: 7492 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2174 2290 2462 2473 2866 3038 3134 Size: 393 542 116 172 11 393 172 96 Cuts at: 3134 3343 3410 4073 4131 4409 4757 5555 6152 Size: 209 67 663 58 278 348 798 597 Cuts at: 6152 6939 7207 1239 Size: 787 268 1524 Fragments arranged by size: 1524 798 787 663 597 542 393 393 348 278 268 209 172 172 116 96 67 58 11 EcoNI CCTnn'n_nnAGG Cuts at: 2806 2993 2806 Size: 187 7305 EcoO109I rG'GnC_Cy Cuts at: 2223 2224 2757 2828 3002 3265 3296 3502 3562 Size: 1 533 71 174 263 31 206 60 Cuts at: 3562 3979 4650 2223 Size: 417 671 5065 Fragments arranged by size: 5065 671 533 417 263 206 174 71 60 31 1 EcoRI G'AATT_C Cuts at: 4801 5179 4801 Size: 378 7114 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2152 2488 2611 4171 2152 Size: 336 123 1560 5473 Fragments arranged by size: 5473 1560 336 123 FspI TGC'GCA Cuts at: 2398 3854 3941 5998 2398 Size: 1456 87 2057 3892 Fragments arranged by size: 3892 2057 1456 87 HaeII r_GCGC'y Cuts at: 1678 2114 2137 3017 3321 3897 6639 1678 Size: 436 23 880 304 576 2742 2531 Fragments arranged by size: 2742 2531 880 576 436 304 23 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2036 2068 2841 2873 3194 Size: 32 135 32 464 32 773 32 321 Cuts at: 3194 3226 3742 3774 6133 6165 6207 6239 1373 Size: 32 516 32 2359 32 42 32 2626 Fragments arranged by size: 2626 2359 773 516 464 321 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4836 5082 669 Size: 988 281 2898 246 3079 Fragments arranged by size: 3079 2898 988 281 246 HindIII A'AGCT_T Cuts at: 748 3821 748 Size: 3073 4419 HpaI GTT'AAC Cuts at: 5082 5082 Size: 7492 KasI G'GCGC_C Cuts at: 2110 3317 2110 Size: 1207 6285 KpnI G_GTAC'C Cuts at: 4817 4817 Size: 7492 MfeI C'AATT_G Cuts at: 5091 5091 Size: 7492 MluI A'CGCG_T Cuts at: 7368 7368 Size: 7492 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6606 6790 1305 Size: 5301 184 2007 Fragments arranged by size: 5301 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2618 3509 10 Size: 54 1425 343 786 891 3993 Fragments arranged by size: 3993 1425 891 786 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3521 3794 3869 3947 3972 4034 5450 Size: 648 2355 273 75 78 25 62 1416 Cuts at: 5450 5809 5968 518 Size: 359 159 2042 Fragments arranged by size: 2355 2042 1416 648 359 273 159 78 75 62 25 MspA1I CmG'CkG Cuts at: 1606 2014 2073 2116 2440 2460 2625 2844 3253 Size: 408 59 43 324 20 165 219 409 Cuts at: 3253 3261 3664 4024 4604 4696 4768 4908 5585 Size: 8 403 360 580 92 72 140 677 Cuts at: 5585 6733 6978 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 580 409 408 403 360 324 245 219 165 140 92 72 59 43 20 8 NarI GG'CG_CC Cuts at: 2111 3318 2111 Size: 1207 6285 NcoI C'CATG_G Cuts at: 513 1065 2004 3867 513 Size: 552 939 1863 4138 Fragments arranged by size: 4138 1863 939 552 NdeI CA'TA_TG Cuts at: 387 2287 387 Size: 1900 5592 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7492 NotI GC'GGCC_GC Cuts at: 4939 4939 Size: 7492 NruI TCG'CGA Cuts at: 3762 3762 Size: 7492 NspI r_CATG'y Cuts at: 1132 1229 3967 4131 4850 6395 1132 Size: 97 2738 164 719 1545 2229 Fragments arranged by size: 2738 2229 1545 719 164 97 PasI CC'CwG_GG Cuts at: 1348 2219 3145 3624 3635 4609 1348 Size: 871 926 479 11 974 4231 Fragments arranged by size: 4231 974 926 871 479 11 PciI A'CATG_T Cuts at: 6391 6391 Size: 7492 PflMI CCAn_nnn'nTGG Cuts at: 1108 2197 3624 1108 Size: 1089 1427 4976 Fragments arranged by size: 4976 1427 1089 PfoI T'CCnGG_A Cuts at: 2489 3558 4158 4254 4425 2489 Size: 1069 600 96 171 5556 Fragments arranged by size: 5556 1069 600 171 96 PmeI GTTT'AAAC Cuts at: 4796 4796 Size: 7492 PmlI CAC'GTG Cuts at: 3229 3229 Size: 7492 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5561 5593 7074 7106 5561 Size: 32 1481 32 5947 Fragments arranged by size: 5947 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3265 3502 3562 3265 Size: 237 60 7195 Fragments arranged by size: 7195 237 60 PshAI GACnn'nnGTC Cuts at: 1613 2091 1613 Size: 478 7014 PsiI TTA'TAA Cuts at: 5062 5062 Size: 7492 PspOMI G'GGCC_C Cuts at: 2223 3002 2223 Size: 779 6713 PspXI vC'TCGA_Gb Cuts at: 3683 3683 Size: 7492 PstI C_TGCA'G Cuts at: 830 2373 2679 3726 4844 830 Size: 1543 306 1047 1118 3478 Fragments arranged by size: 3478 1543 1118 1047 306 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7492 PvuII CAG'CTG Cuts at: 2440 2625 3253 2440 Size: 185 628 6679 Fragments arranged by size: 6679 628 185 SacI G_AGCT'C Cuts at: 4065 4065 Size: 7492 SacII CC_GC'GG Cuts at: 2015 2117 2015 Size: 102 7390 SalI G'TCGA_C Cuts at: 1936 4834 1936 Size: 2898 4594 SapI GCTCTTCn'nnn_ Cuts at: 2159 4377 2159 Size: 2218 5274 SbfI CC_TGCA'GG Cuts at: 4844 4844 Size: 7492 ScaI AGT'ACT Cuts at: 2351 5740 7452 2351 Size: 3389 1712 2391 Fragments arranged by size: 3389 2391 1712 SfcI C'TryA_G Cuts at: 826 1045 2369 2675 3722 4186 4840 5975 6656 Size: 219 1324 306 1047 464 654 1135 681 Cuts at: 6656 6847 826 Size: 191 1471 Fragments arranged by size: 1471 1324 1135 1047 681 654 464 306 219 191 SfoI GGC'GCC Cuts at: 2112 3319 2112 Size: 1207 6285 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7492 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7492 SmaI CCC'GGG Cuts at: 1872 2142 4819 1872 Size: 270 2677 4545 Fragments arranged by size: 4545 2677 270 SmlI C'TyrA_G Cuts at: 820 1017 1943 3405 3683 4004 4374 5596 6497 Size: 197 926 1462 278 321 370 1222 901 Cuts at: 6497 6759 820 Size: 262 1553 Fragments arranged by size: 1553 1462 1222 926 901 370 321 278 262 197 SnaBI TAC'GTA Cuts at: 493 5283 493 Size: 4790 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7492 SphI G_CATG'C Cuts at: 1132 3967 4850 1132 Size: 2835 883 3774 Fragments arranged by size: 3774 2835 883 SspI AAT'ATT Cuts at: 5 52 5416 5 Size: 47 5364 2081 Fragments arranged by size: 5364 2081 47 StuI AGG'CCT Cuts at: 1517 4013 1517 Size: 2496 4996 StyI C'CwwG_G Cuts at: 513 1065 2004 3006 3498 3783 3867 4931 513 Size: 552 939 1002 492 285 84 1064 3074 Fragments arranged by size: 3074 1064 1002 939 552 492 285 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3321 4212 4351 5519 5536 5689 5874 Size: 1255 1741 891 139 1168 17 153 185 Cuts at: 5874 6319 325 Size: 445 1498 Fragments arranged by size: 1741 1498 1255 1168 891 445 185 153 139 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2258 2349 5738 Size: 274 80 33 51 1443 281 91 3389 Cuts at: 5738 7450 96 Size: 1712 138 Fragments arranged by size: 3389 1712 1443 281 274 138 91 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2540 2572 7452 7484 2540 Size: 32 4880 32 2548 Fragments arranged by size: 4880 2548 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7492 XbaI T'CTAG_A Cuts at: 4828 4828 Size: 7492 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3013 3142 3709 3013 Size: 129 567 6796 Fragments arranged by size: 6796 567 129 XhoI C'TCGA_G Cuts at: 1943 3405 3683 1943 Size: 1462 278 5752 Fragments arranged by size: 5752 1462 278 XmaI C'CCGG_G Cuts at: 1870 2140 4817 1870 Size: 270 2677 4545 Fragments arranged by size: 4545 2677 270 XmnI GAAnn'nnTTC Cuts at: 2576 4412 5621 2576 Size: 1836 1209 4447 Fragments arranged by size: 4447 1836 1209 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3708 5300 276 Size: 53 83 186 936 2174 1592 2468 Fragments arranged by size: 2468 2174 1592 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NsiI PacI PsrI RsrII SanDI SexAI SfiI SrfI SwaI