(Linear) (Six-base) MAPSORT of: KIEE3507.seq Check: 2070 from: 1 to: 2787 KIEE3507.orf With 182 enzymes: SgfI * July 3, 2012 18:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1710 1785 2787 Size: 1710 75 1002 Fragments arranged by size: 1710 1002 75 AatII G_ACGT'C Cuts at: 0 1705 2787 Size: 1705 1082 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1129 1338 2787 Size: 1129 209 1449 Fragments arranged by size: 1449 1129 209 AflIII A'CryG_T Cuts at: 0 311 2787 Size: 311 2476 AgeI A'CCGG_T Cuts at: 0 1322 2787 Size: 1322 1465 AhdI GACnn_n'nnGTC Cuts at: 0 45 1751 2787 Size: 45 1706 1036 Fragments arranged by size: 1706 1036 45 AleI CACnn'nnGTG Cuts at: 0 1864 2029 2787 Size: 1864 165 758 Fragments arranged by size: 1864 758 165 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1861 2372 2497 2787 Size: 1861 511 125 290 Fragments arranged by size: 1861 511 290 125 AlwNI CAG_nnn'CTG Cuts at: 0 192 1294 1560 1601 2133 2427 2787 Size: 192 1102 266 41 532 294 360 Fragments arranged by size: 1102 532 360 294 266 192 41 ApaI G_GGCC'C Cuts at: 0 222 1001 2787 Size: 222 779 1786 Fragments arranged by size: 1786 779 222 ApaLI G'TGCA_C Cuts at: 0 412 1030 2787 Size: 412 618 1757 Fragments arranged by size: 1757 618 412 ApoI r'AATT_y Cuts at: 0 447 1420 2787 Size: 447 973 1367 Fragments arranged by size: 1367 973 447 AvaI C'yCGr_G Cuts at: 0 22 91 135 1282 1400 1678 2787 Size: 22 69 44 1147 118 278 1109 Fragments arranged by size: 1147 1109 278 118 69 44 22 BamHI G'GATC_C Cuts at: 0 734 1043 1079 2787 Size: 734 309 36 1708 Fragments arranged by size: 1708 734 309 36 BanI G'GyrC_C Cuts at: 0 105 515 1312 1609 1920 2787 Size: 105 410 797 297 311 867 Fragments arranged by size: 867 797 410 311 297 105 BanII G_rGCy'C Cuts at: 0 222 1001 1277 1655 1811 2060 2096 2199 Size: 222 779 276 378 156 249 36 103 Cuts at: 2199 2787 Size: 588 Fragments arranged by size: 779 588 378 276 249 222 156 103 36 BbeI G_GCGC'C Cuts at: 0 109 1316 2787 Size: 109 1207 1471 Fragments arranged by size: 1471 1207 109 BbsI GAAGACnn'nnnn_ Cuts at: 0 969 1332 2665 2787 Size: 969 363 1333 122 Fragments arranged by size: 1333 969 363 122 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 371 405 1821 1855 2787 Size: 371 34 1416 34 932 Fragments arranged by size: 1416 932 371 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 545 1659 2787 Size: 545 1114 1128 Fragments arranged by size: 1128 1114 545 BlpI GC'TnA_GC Cuts at: 0 1271 2787 Size: 1271 1516 Bme1580I G_kGCm'C Cuts at: 0 158 222 416 1001 1034 1612 2026 2070 Size: 158 64 194 585 33 578 414 44 Cuts at: 2070 2787 Size: 717 Fragments arranged by size: 717 585 578 414 194 158 64 44 33 BmgBI CAC'GTC Cuts at: 0 1377 2787 Size: 1377 1410 BmrI ACTGGGnnnn_n' Cuts at: 0 531 1185 2787 Size: 531 654 1602 Fragments arranged by size: 1602 654 531 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 708 2787 Size: 708 2079 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 169 285 457 468 861 1033 1405 2068 Size: 169 116 172 11 393 172 372 663 Cuts at: 2068 2126 2404 2752 2787 Size: 58 278 348 35 Fragments arranged by size: 663 393 372 348 278 172 172 169 116 58 35 11 Bpu10I CC'TnA_GC Cuts at: 0 985 2258 2705 2787 Size: 985 1273 447 82 Fragments arranged by size: 1273 985 447 82 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1984 2354 2787 Size: 1984 370 433 Fragments arranged by size: 1984 433 370 BsaI GGTCTCn'nnnn_ Cuts at: 0 298 837 2572 2787 Size: 298 539 1735 215 Fragments arranged by size: 1735 539 298 215 BsaAI yAC'GTr Cuts at: 0 1224 2787 Size: 1224 1563 BsaBI GATnn'nnATC Cuts at: 0 2245 2787 Size: 2245 542 BsaHI Gr'CG_yC Cuts at: 0 83 106 537 1313 1370 1702 2159 2787 Size: 83 23 431 776 57 332 457 628 Fragments arranged by size: 776 628 457 431 332 83 57 23 BsaWI w'CCGG_w Cuts at: 0 248 1322 2787 Size: 248 1074 1465 Fragments arranged by size: 1465 1074 248 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 697 844 1243 2787 Size: 697 147 399 1544 Fragments arranged by size: 1544 697 399 147 BseYI C'CCAG_C Cuts at: 0 696 1256 1655 2019 2265 2437 2787 Size: 696 560 399 364 246 172 350 Fragments arranged by size: 696 560 399 364 350 246 172 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 246 1813 1885 1888 1963 2345 2413 2467 Size: 246 1567 72 3 75 382 68 54 Cuts at: 2467 2787 Size: 320 Fragments arranged by size: 1567 382 320 246 75 72 68 54 3 BsiEI CG_ry'CG Cuts at: 0 1323 2193 2787 Size: 1323 870 594 Fragments arranged by size: 1323 870 594 BsiHKAI G_wGCw'C Cuts at: 0 293 416 694 1034 1640 1769 2060 2207 Size: 293 123 278 340 606 129 291 147 Cuts at: 2207 2787 Size: 580 Fragments arranged by size: 606 580 340 293 291 278 147 129 123 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2453 2770 2787 Size: 2453 317 17 Fragments arranged by size: 2453 317 17 Bsp1286I G_dGCh'C Cuts at: 0 158 222 293 416 694 1001 1034 1277 Size: 158 64 71 123 278 307 33 243 Cuts at: 1277 1612 1640 1655 1769 1811 2026 2060 2070 Size: 335 28 15 114 42 215 34 10 Cuts at: 2070 2096 2199 2207 2787 Size: 26 103 8 580 Fragments arranged by size: 580 335 307 278 243 215 158 123 114 103 71 64 42 34 33 28 26 15 10 8 BspEI T'CCGG_A Cuts at: 0 248 2787 Size: 248 2539 BspHI T'CATG_A Cuts at: 0 878 2654 2787 Size: 878 1776 133 Fragments arranged by size: 1776 878 133 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1046 1710 1785 2534 2787 Size: 1046 664 75 749 253 Fragments arranged by size: 1046 749 664 253 75 BsrBI CCG'CTC Cuts at: 0 240 876 1740 2787 Size: 240 636 864 1047 Fragments arranged by size: 1047 864 636 240 BsrFI r'CCGG_y Cuts at: 0 85 1322 1917 2738 2787 Size: 85 1237 595 821 49 Fragments arranged by size: 1237 821 595 85 49 BssSI C'ACGA_G Cuts at: 0 624 1393 2787 Size: 624 769 1394 Fragments arranged by size: 1394 769 624 BstAPI GCAn_nnn'nTGC Cuts at: 0 1038 1920 2621 2787 Size: 1038 882 701 166 Fragments arranged by size: 1038 882 701 166 BstXI CCAn_nnnn'nTGG Cuts at: 0 1500 2787 Size: 1500 1287 BstYI r'GATC_y Cuts at: 0 245 734 1043 1079 1211 1550 2549 2787 Size: 245 489 309 36 132 339 999 238 Fragments arranged by size: 999 489 339 309 245 238 132 36 Bsu36I CC'TnA_GG Cuts at: 0 827 1295 2787 Size: 827 468 1492 Fragments arranged by size: 1492 827 468 BtgI C'CryG_G Cuts at: 0 7 109 713 1862 2787 Size: 7 102 604 1149 925 Fragments arranged by size: 1149 925 604 102 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1824 2787 Size: 1824 963 BtsI GCAGTG_nn' Cuts at: 0 1756 2165 2344 2787 Size: 1756 409 179 443 Fragments arranged by size: 1756 443 409 179 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1161 1196 2787 Size: 1161 35 1591 Fragments arranged by size: 1591 1161 35 DrdI GACnn_nn'nnGTC Cuts at: 0 312 1375 2787 Size: 312 1063 1412 Fragments arranged by size: 1412 1063 312 EaeI y'GGCC_r Cuts at: 0 611 1502 1709 2720 2787 Size: 611 891 207 1011 67 Fragments arranged by size: 1011 891 611 207 67 EarI CTCTTCn'nnn_ Cuts at: 0 154 781 2372 2787 Size: 154 627 1591 415 Fragments arranged by size: 1591 627 415 154 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 45 194 1742 1899 2787 Size: 45 149 1548 157 888 Fragments arranged by size: 1548 888 157 149 45 EcoICRI GAG'CTC Cuts at: 0 2058 2787 Size: 2058 729 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 169 285 457 468 861 1033 1129 1338 Size: 169 116 172 11 393 172 96 209 Cuts at: 1338 1405 2068 2126 2404 2752 2787 Size: 67 663 58 278 348 35 Fragments arranged by size: 663 393 348 278 209 172 172 169 116 96 67 58 35 11 EcoNI CCTnn'n_nnAGG Cuts at: 0 801 988 2787 Size: 801 187 1799 Fragments arranged by size: 1799 801 187 EcoO109I rG'GnC_Cy Cuts at: 0 218 219 752 823 997 1260 1291 1497 Size: 218 1 533 71 174 263 31 206 Cuts at: 1497 1557 1974 2645 2787 Size: 60 417 671 142 Fragments arranged by size: 671 533 417 263 218 206 174 142 71 60 31 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 147 483 606 2166 2787 Size: 147 336 123 1560 621 Fragments arranged by size: 1560 621 336 147 123 FspI TGC'GCA Cuts at: 0 393 1849 1936 2787 Size: 393 1456 87 851 Fragments arranged by size: 1456 851 393 87 HaeII r_GCGC'y Cuts at: 0 109 132 1012 1316 1892 2787 Size: 109 23 880 304 576 895 Fragments arranged by size: 895 880 576 304 109 23 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 31 63 836 868 1189 1221 1737 1769 Size: 31 32 773 32 321 32 516 32 Cuts at: 1769 2787 Size: 1018 Fragments arranged by size: 1018 773 516 321 32 32 32 32 31 HindIII A'AGCT_T Cuts at: 0 1816 2787 Size: 1816 971 KasI G'GCGC_C Cuts at: 0 105 1312 2787 Size: 105 1207 1475 Fragments arranged by size: 1475 1207 105 MscI TGG'CCA Cuts at: 0 613 1504 2787 Size: 613 891 1283 Fragments arranged by size: 1283 891 613 MslI CAynn'nnrTG Cuts at: 0 1516 1789 1864 1942 1967 2029 2787 Size: 1516 273 75 78 25 62 758 Fragments arranged by size: 1516 758 273 78 75 62 25 MspA1I CmG'CkG Cuts at: 0 9 68 111 435 455 620 839 1248 Size: 9 59 43 324 20 165 219 409 Cuts at: 1248 1256 1659 2019 2599 2691 2763 2787 Size: 8 403 360 580 92 72 24 Fragments arranged by size: 580 409 403 360 324 219 165 92 72 59 43 24 20 9 8 NarI GG'CG_CC Cuts at: 0 106 1313 2787 Size: 106 1207 1474 Fragments arranged by size: 1474 1207 106 NcoI C'CATG_G Cuts at: 0 1862 2787 Size: 1862 925 NdeI CA'TA_TG Cuts at: 0 282 2787 Size: 282 2505 NruI TCG'CGA Cuts at: 0 1757 2787 Size: 1757 1030 NspI r_CATG'y Cuts at: 0 1962 2126 2787 Size: 1962 164 661 Fragments arranged by size: 1962 661 164 PasI CC'CwG_GG Cuts at: 0 214 1140 1619 1630 2604 2787 Size: 214 926 479 11 974 183 Fragments arranged by size: 974 926 479 214 183 11 PflMI CCAn_nnn'nTGG Cuts at: 0 192 1619 2787 Size: 192 1427 1168 Fragments arranged by size: 1427 1168 192 PfoI T'CCnGG_A Cuts at: 0 484 1553 2153 2249 2420 2787 Size: 484 1069 600 96 171 367 Fragments arranged by size: 1069 600 484 367 171 96 PmlI CAC'GTG Cuts at: 0 1224 2787 Size: 1224 1563 PpuMI rG'GwC_Cy Cuts at: 0 1260 1497 1557 2787 Size: 1260 237 60 1230 Fragments arranged by size: 1260 1230 237 60 PshAI GACnn'nnGTC Cuts at: 0 86 2787 Size: 86 2701 PspOMI G'GGCC_C Cuts at: 0 218 997 2787 Size: 218 779 1790 Fragments arranged by size: 1790 779 218 PspXI vC'TCGA_Gb Cuts at: 0 1678 2787 Size: 1678 1109 PstI C_TGCA'G Cuts at: 0 368 674 1721 2787 Size: 368 306 1047 1066 Fragments arranged by size: 1066 1047 368 306 PvuII CAG'CTG Cuts at: 0 435 620 1248 2787 Size: 435 185 628 1539 Fragments arranged by size: 1539 628 435 185 SacI G_AGCT'C Cuts at: 0 2060 2787 Size: 2060 727 SacII CC_GC'GG Cuts at: 0 10 112 2787 Size: 10 102 2675 Fragments arranged by size: 2675 102 10 SapI GCTCTTCn'nnn_ Cuts at: 0 154 2372 2787 Size: 154 2218 415 Fragments arranged by size: 2218 415 154 ScaI AGT'ACT Cuts at: 0 346 2787 Size: 346 2441 SfcI C'TryA_G Cuts at: 0 364 670 1717 2181 2787 Size: 364 306 1047 464 606 Fragments arranged by size: 1047 606 464 364 306 SfoI GGC'GCC Cuts at: 0 107 1314 2787 Size: 107 1207 1473 Fragments arranged by size: 1473 1207 107 SmaI CCC'GGG Cuts at: 0 137 2787 Size: 137 2650 SmlI C'TyrA_G Cuts at: 0 1400 1678 1999 2369 2787 Size: 1400 278 321 370 418 Fragments arranged by size: 1400 418 370 321 278 SphI G_CATG'C Cuts at: 0 1962 2787 Size: 1962 825 StuI AGG'CCT Cuts at: 0 2008 2787 Size: 2008 779 StyI C'CwwG_G Cuts at: 0 1001 1493 1778 1862 2787 Size: 1001 492 285 84 925 Fragments arranged by size: 1001 925 492 285 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1316 2207 2346 2787 Size: 1316 891 139 441 Fragments arranged by size: 1316 891 441 139 TatI w'GTAC_w Cuts at: 0 253 344 2787 Size: 253 91 2443 Fragments arranged by size: 2443 253 91 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 535 567 2787 Size: 535 32 2220 Fragments arranged by size: 2220 535 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1008 1137 1704 2787 Size: 1008 129 567 1083 Fragments arranged by size: 1083 1008 567 129 XhoI C'TCGA_G Cuts at: 0 1400 1678 2787 Size: 1400 278 1109 Fragments arranged by size: 1400 1109 278 XmaI C'CCGG_G Cuts at: 0 135 2787 Size: 135 2652 XmnI GAAnn'nnTTC Cuts at: 0 571 2407 2787 Size: 571 1836 380 Fragments arranged by size: 1836 571 380 ZraI GAC'GTC Cuts at: 0 1703 2787 Size: 1703 1084 Enzymes that do cut: AarI AatII AcuI AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BcgI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HindIII KasI MscI MslI MspA1I NarI NcoI NdeI NruI NspI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI PstI PvuII SacI SacII SapI ScaI SfcI SfoI SmaI SmlI SphI StuI StyI TaqII TatI TstI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflII AloI AscI AseI AsiSI AvrII BaeI BbvCI BciVI BclI BfrBI BglII BmtI BsaXI BsiWI BsmI BsrDI BsrGI BssHII BstBI BstEII BstZ17I ClaI DraI DraIII EagI EcoRI EcoRV FseI FspAI HincII HpaI KpnI MfeI MluI MmeI NaeI NgoMIV NheI NotI NsiI PacI PciI PmeI PpiI PsiI PsrI PvuI RsrII SalI SanDI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI