(Circular) (Six-base) MAPSORT of: pFN21AE3509.seq Check: 353 from: 1 to: 6445 pFN21AE3509 6445 bp With 182 enzymes: SgfI * July 6, 2012 17:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2913 3483 2913 Size: 570 5875 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4255 278 Size: 53 83 186 936 2719 2468 Fragments arranged by size: 2719 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3788 1937 Size: 1851 4594 Acc65I G'GTAC_C Cuts at: 2645 3766 2645 Size: 1121 5324 AclI AA'CG_TT Cuts at: 1563 3596 4572 4945 1563 Size: 2033 976 373 3063 Fragments arranged by size: 3063 2033 976 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3723 4508 5892 6160 1632 Size: 2091 785 1384 268 1917 Fragments arranged by size: 2091 1917 1384 785 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6248 AflIII A'CryG_T Cuts at: 1203 5344 6321 1203 Size: 4141 977 1327 Fragments arranged by size: 4141 1327 977 AgeI A'CCGG_T Cuts at: 5276 5276 Size: 6445 AhdI GACnn_n'nnGTC Cuts at: 5174 5174 Size: 6445 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6445 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3314 1184 Size: 2130 4315 AlwNI CAG_nnn'CTG Cuts at: 3737 4188 5760 3737 Size: 451 1572 4422 Fragments arranged by size: 4422 1572 451 ApaI G_GGCC'C Cuts at: 3729 3729 Size: 6445 ApaLI G'TGCA_C Cuts at: 4502 5658 4502 Size: 1156 5289 ApoI r'AATT_y Cuts at: 1493 3125 3754 3980 4132 6241 1493 Size: 1632 629 226 152 2109 1697 Fragments arranged by size: 2109 1697 1632 629 226 152 AseI AT'TA_AT Cuts at: 160 1032 4999 160 Size: 872 3967 1606 Fragments arranged by size: 3967 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6445 AvaI C'yCGr_G Cuts at: 1870 1943 2622 3539 3770 1870 Size: 73 679 917 231 4545 Fragments arranged by size: 4545 917 679 231 73 BamHI G'GATC_C Cuts at: 3775 4142 3775 Size: 367 6078 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2645 3766 5221 618 Size: 325 217 627 858 1121 1455 1842 Fragments arranged by size: 1842 1455 1121 858 627 325 217 BanII G_rGCy'C Cuts at: 1391 2782 3281 3510 3729 3764 1391 Size: 1391 499 229 219 35 4072 Fragments arranged by size: 4072 1391 499 229 219 35 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2434 2701 928 Size: 971 535 267 4672 Fragments arranged by size: 4672 971 535 267 BbvCI CC'TCA_GC Cuts at: 3572 3572 Size: 6445 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4636 4670 1903 Size: 34 2699 34 3678 Fragments arranged by size: 3678 2699 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4337 5547 4337 Size: 1210 5235 BclI T'GATC_A Cuts at: 1548 1548 Size: 6445 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3336 5056 136 Size: 107 122 71 631 605 1664 1720 1525 Fragments arranged by size: 1720 1664 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2808 6440 2808 Size: 3632 2813 BlpI GC'TnA_GC Cuts at: 3269 3862 3269 Size: 593 5852 Bme1580I G_kGCm'C Cuts at: 1790 3403 3729 4506 5662 1790 Size: 1613 326 777 1156 2573 Fragments arranged by size: 2573 1613 1156 777 326 BmgBI CAC'GTC Cuts at: 1318 2440 1318 Size: 1122 5323 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2162 4176 5134 Size: 416 53 83 399 24 745 2014 958 Cuts at: 5134 442 Size: 1753 Fragments arranged by size: 2014 1753 958 745 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2402 1056 Size: 1346 5099 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2310 5105 1239 Size: 1071 2795 2579 Fragments arranged by size: 2795 2579 1071 Bpu10I CC'TnA_GC Cuts at: 2030 3504 3572 2030 Size: 1474 68 4903 Fragments arranged by size: 4903 1474 68 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4570 5435 5733 4570 Size: 865 298 5282 Fragments arranged by size: 5282 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2169 2497 2762 2825 3734 5108 882 Size: 617 670 328 265 63 909 1374 2219 Fragments arranged by size: 2219 1374 909 670 617 328 265 63 BsaAI yAC'GTr Cuts at: 493 1204 2458 4236 493 Size: 711 1254 1778 2702 Fragments arranged by size: 2702 1778 1254 711 BsaBI GATnn'nnATC Cuts at: 2117 6439 2117 Size: 4322 2123 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4252 4634 5269 275 Size: 53 83 186 936 2719 382 635 1451 Fragments arranged by size: 2719 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4878 5276 5550 5697 6108 4878 Size: 398 274 147 411 5215 Fragments arranged by size: 5215 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6220 Fragments arranged by size: 6220 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2050 2780 3578 1186 Size: 864 730 798 4053 Fragments arranged by size: 4053 864 798 730 BseYI C'CCAG_C Cuts at: 2772 2835 3301 3320 3509 3730 5648 6144 2772 Size: 63 466 19 189 221 1918 496 3073 Fragments arranged by size: 3073 1918 496 466 221 189 63 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5872 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3895 4656 5684 1540 Size: 275 186 1894 761 1028 2301 Fragments arranged by size: 2301 1894 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3094 3281 3529 4506 4591 5662 3094 Size: 187 248 977 85 1071 3877 Fragments arranged by size: 3877 1071 977 248 187 85 BsmI GAATG_Cn' Cuts at: 3956 4049 3956 Size: 93 6352 BsmBI CGTCTCn'nnnn_ Cuts at: 2236 2236 Size: 6445 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2782 3094 3281 3403 3510 3529 3729 Size: 399 992 312 187 122 107 19 200 Cuts at: 3729 3764 4506 4591 5662 1391 Size: 35 742 85 1071 2174 Fragments arranged by size: 2174 1071 992 742 399 312 200 187 122 107 85 35 19 BspEI T'CCGG_A Cuts at: 6108 6108 Size: 6445 BspHI T'CATG_A Cuts at: 4334 6064 4334 Size: 1730 4715 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2207 2904 2913 3228 3483 3800 844 Size: 1363 697 9 315 255 317 3489 Fragments arranged by size: 3489 1363 697 317 315 255 9 BsrBI CCG'CTC Cuts at: 1394 1823 4332 1394 Size: 429 2509 3507 Fragments arranged by size: 3507 2509 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4940 5114 65 Size: 1183 3692 174 1396 Fragments arranged by size: 3692 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5089 5276 1700 Size: 110 3279 187 2869 Fragments arranged by size: 3279 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6445 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6445 BssSI C'ACGA_G Cuts at: 4505 5517 4505 Size: 1012 5433 BstAPI GCAn_nnn'nTGC Cuts at: 794 2752 794 Size: 1958 4487 BstBI TT'CG_AA Cuts at: 5249 5249 Size: 6445 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6445 BstXI CCAn_nnnn'nTGG Cuts at: 2354 3490 2354 Size: 1136 5309 BstYI r'GATC_y Cuts at: 1972 2808 3078 3579 3775 4142 4527 4544 5996 Size: 836 270 501 196 367 385 17 1452 Cuts at: 5996 6082 6094 6440 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 836 501 385 367 346 270 196 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2876 2876 Size: 6445 BtgI C'CryG_G Cuts at: 513 1065 2004 2404 513 Size: 552 939 400 4554 Fragments arranged by size: 4554 939 552 400 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2238 487 Size: 683 56 1012 4694 Fragments arranged by size: 4694 1012 683 56 BtsI GCAGTG_nn' Cuts at: 2519 3065 3961 4755 4775 2519 Size: 546 896 794 20 4189 Fragments arranged by size: 4189 896 794 546 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6445 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6410 DraI TTT'AAA Cuts at: 3749 3749 Size: 6445 DraIII CAC_nnn'GTG Cuts at: 2218 3240 2218 Size: 1022 5423 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2250 5452 809 Size: 507 934 3202 1802 Fragments arranged by size: 3202 1802 934 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2191 2503 2616 Size: 54 1425 50 275 18 361 312 113 Cuts at: 2616 3130 3651 3892 4781 6410 8 Size: 514 521 241 889 1629 43 Fragments arranged by size: 1629 1425 889 521 514 361 312 275 241 113 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3892 1537 Size: 275 2080 4090 Fragments arranged by size: 4090 2080 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2042 2082 2087 2300 2474 4375 1353 Size: 689 40 5 213 174 1901 3423 Fragments arranged by size: 3423 1901 689 213 174 40 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5032 5406 5552 387 Size: 1064 138 3443 374 146 1280 Fragments arranged by size: 3443 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3279 3279 Size: 6445 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2310 3723 4508 5105 5892 6160 1239 Size: 393 678 1413 785 597 787 268 1524 Fragments arranged by size: 1524 1413 787 785 678 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2795 2879 2795 Size: 84 6361 EcoO109I rG'GnC_Cy Cuts at: 2335 2463 3015 3382 3535 3679 3725 3726 2335 Size: 128 552 367 153 144 46 1 5054 Fragments arranged by size: 5054 552 367 153 144 128 46 1 EcoRI G'AATT_C Cuts at: 3754 4132 3754 Size: 378 6067 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2076 3685 2076 Size: 1609 4836 FspI TGC'GCA Cuts at: 4951 4951 Size: 6445 HaeII r_GCGC'y Cuts at: 1678 5592 1678 Size: 3914 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2552 2584 5086 5118 5160 Size: 32 135 32 980 32 2502 32 42 Cuts at: 5160 5192 1373 Size: 32 2626 Fragments arranged by size: 2626 2502 980 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3789 4035 669 Size: 988 281 1851 246 3079 Fragments arranged by size: 3079 1851 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6445 HpaI GTT'AAC Cuts at: 4035 4035 Size: 6445 KpnI G_GTAC'C Cuts at: 2649 3770 2649 Size: 1121 5324 MfeI C'AATT_G Cuts at: 4044 4044 Size: 6445 MluI A'CGCG_T Cuts at: 6321 6321 Size: 6445 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2952 5559 5743 1305 Size: 1647 2607 184 2007 Fragments arranged by size: 2607 2007 1647 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2618 10 Size: 54 1425 343 786 3837 Fragments arranged by size: 3837 1425 786 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4403 4762 4921 518 Size: 648 3237 359 159 2042 Fragments arranged by size: 3237 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2704 2776 2869 3226 3861 4538 5686 5931 Size: 1098 72 93 357 635 677 1148 245 Cuts at: 5931 1606 Size: 2120 Fragments arranged by size: 2120 1148 1098 677 635 357 245 93 72 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4954 Fragments arranged by size: 4954 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6445 NheI G'CTAG_C Cuts at: 1052 2398 1052 Size: 1346 5099 NotI GC'GGCC_GC Cuts at: 3892 3892 Size: 6445 NruI TCG'CGA Cuts at: 3003 3003 Size: 6445 NspI r_CATG'y Cuts at: 1132 1229 3691 3803 5348 1132 Size: 97 2462 112 1545 2229 Fragments arranged by size: 2462 2229 1545 112 97 PasI CC'CwG_GG Cuts at: 1348 2990 1348 Size: 1642 4803 PciI A'CATG_T Cuts at: 5344 5344 Size: 6445 PflMI CCAn_nnn'nTGG Cuts at: 1108 3262 1108 Size: 2154 4291 PmeI GTTT'AAAC Cuts at: 3749 3749 Size: 6445 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4514 4546 6027 6059 4514 Size: 32 1481 32 4900 Fragments arranged by size: 4900 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2463 3015 3382 3535 2463 Size: 552 367 153 5373 Fragments arranged by size: 5373 552 367 153 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6445 PsiI TTA'TAA Cuts at: 4015 4015 Size: 6445 PspOMI G'GGCC_C Cuts at: 3725 3725 Size: 6445 PstI C_TGCA'G Cuts at: 830 2691 3225 3696 3797 830 Size: 1861 534 471 101 3478 Fragments arranged by size: 3478 1861 534 471 101 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6445 PvuII CAG'CTG Cuts at: 2869 2869 Size: 6445 SacI G_AGCT'C Cuts at: 3281 3281 Size: 6445 SalI G'TCGA_C Cuts at: 1936 3787 1936 Size: 1851 4594 SanDI GG'GwC_CC Cuts at: 2463 3015 2463 Size: 552 5893 SbfI CC_TGCA'GG Cuts at: 3797 3797 Size: 6445 ScaI AGT'ACT Cuts at: 4693 6405 4693 Size: 1712 4733 SfcI C'TryA_G Cuts at: 826 1045 2687 3221 3692 3793 4928 5609 5800 Size: 219 1642 534 471 101 1135 681 191 Cuts at: 5800 826 Size: 1471 Fragments arranged by size: 1642 1471 1135 681 534 471 219 191 101 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6445 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6445 SmaI CCC'GGG Cuts at: 1872 3541 3772 1872 Size: 1669 231 4545 Fragments arranged by size: 4545 1669 231 SmlI C'TyrA_G Cuts at: 820 1017 1943 4549 5450 5712 820 Size: 197 926 2606 901 262 1553 Fragments arranged by size: 2606 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4236 493 Size: 3743 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6445 SphI G_CATG'C Cuts at: 1132 3803 1132 Size: 2671 3774 SspI AAT'ATT Cuts at: 5 52 4369 5 Size: 47 4317 2081 Fragments arranged by size: 4317 2081 47 StuI AGG'CCT Cuts at: 1517 3557 1517 Size: 2040 4405 StyI C'CwwG_G Cuts at: 513 1065 2004 2430 2556 2576 3109 3345 3884 Size: 552 939 426 126 20 533 236 539 Cuts at: 3884 513 Size: 3074 Fragments arranged by size: 3074 939 552 539 533 426 236 126 20 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3207 4472 4489 4642 4827 5272 325 Size: 1255 1627 1265 17 153 185 445 1498 Fragments arranged by size: 1627 1498 1265 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4691 6403 96 Size: 274 80 33 51 1443 2714 1712 138 Fragments arranged by size: 2714 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2222 2254 2564 2596 6405 6437 2222 Size: 32 310 32 3809 32 2230 Fragments arranged by size: 3809 2230 310 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2262 1316 Size: 946 5499 XbaI T'CTAG_A Cuts at: 3781 3781 Size: 6445 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3490 3490 Size: 6445 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6445 XmaI C'CCGG_G Cuts at: 1870 3539 3770 1870 Size: 1669 231 4545 Fragments arranged by size: 4545 1669 231 XmnI GAAnn'nnTTC Cuts at: 3665 4574 3665 Size: 909 5536 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4253 276 Size: 53 83 186 936 2719 2468 Fragments arranged by size: 2719 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI