Restriction map for
pFN21AE3509
(Linear) (Six-base) MAPSORT of: KIEE3509.seq Check: 5964 from: 1 to: 1740
KIEE3509.orf
With 182 enzymes: SgfI *
July 3, 2012 18:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 908 1478 1740
Size: 908 570 262
Fragments arranged by size:
908 570 262
Acc65I G'GTAC_C
Cuts at: 0 640 1740
Size: 640 1100
AclI AA'CG_TT
Cuts at: 0 1591 1740
Size: 1591 149
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1718 1740
Size: 1718 22
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1309 1740
Size: 1309 431
AlwNI CAG_nnn'CTG
Cuts at: 0 1732 1740
Size: 1732 8
ApaI G_GGCC'C
Cuts at: 0 1724 1740
Size: 1724 16
ApoI r'AATT_y
Cuts at: 0 1120 1740
Size: 1120 620
AvaI C'yCGr_G
Cuts at: 0 617 1534 1740
Size: 617 917 206
Fragments arranged by size:
917 617 206
BanI G'GyrC_C
Cuts at: 0 640 1740
Size: 640 1100
BanII G_rGCy'C
Cuts at: 0 777 1276 1505 1724 1740
Size: 777 499 229 219 16
Fragments arranged by size:
777 499 229 219 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 429 696 1740
Size: 429 267 1044
Fragments arranged by size:
1044 429 267
BbvCI CC'TCA_GC
Cuts at: 0 1567 1740
Size: 1567 173
BglI GCCn_nnn'nGGC
Cuts at: 0 1331 1740
Size: 1331 409
BglII A'GATC_T
Cuts at: 0 803 1740
Size: 803 937
BlpI GC'TnA_GC
Cuts at: 0 1264 1740
Size: 1264 476
Bme1580I G_kGCm'C
Cuts at: 0 1398 1724 1740
Size: 1398 326 16
Fragments arranged by size:
1398 326 16
BmgBI CAC'GTC
Cuts at: 0 435 1740
Size: 435 1305
BmrI ACTGGGnnnn_n'
Cuts at: 0 157 1740
Size: 157 1583
BmtI G_CTAG'C
Cuts at: 0 397 1740
Size: 397 1343
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 1740
Size: 305 1435
Bpu10I CC'TnA_GC
Cuts at: 0 25 1499 1567 1740
Size: 25 1474 68 173
Fragments arranged by size:
1474 173 68 25
BsaI GGTCTCn'nnnn_
Cuts at: 0 164 492 757 820 1729 1740
Size: 164 328 265 63 909 11
Fragments arranged by size:
909 328 265 164 63 11
BsaAI yAC'GTr
Cuts at: 0 453 1740
Size: 453 1287
BsaBI GATnn'nnATC
Cuts at: 0 112 1740
Size: 112 1628
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 45 775 1573 1740
Size: 45 730 798 167
Fragments arranged by size:
798 730 167 45
BseYI C'CCAG_C
Cuts at: 0 767 830 1296 1315 1504 1725 1740
Size: 767 63 466 19 189 221 15
Fragments arranged by size:
767 466 221 189 63 19 15
BsiHKAI G_wGCw'C
Cuts at: 0 1089 1276 1524 1740
Size: 1089 187 248 216
Fragments arranged by size:
1089 248 216 187
BsmBI CGTCTCn'nnnn_
Cuts at: 0 231 1740
Size: 231 1509
Bsp1286I G_dGCh'C
Cuts at: 0 777 1089 1276 1398 1505 1524 1724 1740
Size: 777 312 187 122 107 19 200 16
Fragments arranged by size:
777 312 200 187 122 107 19 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 202 899 908 1223 1478 1740
Size: 202 697 9 315 255 262
Fragments arranged by size:
697 315 262 255 202 9
BstAPI GCAn_nnn'nTGC
Cuts at: 0 747 1740
Size: 747 993
BstXI CCAn_nnnn'nTGG
Cuts at: 0 349 1485 1740
Size: 349 1136 255
Fragments arranged by size:
1136 349 255
BstYI r'GATC_y
Cuts at: 0 803 1073 1574 1740
Size: 803 270 501 166
Fragments arranged by size:
803 501 270 166
Bsu36I CC'TnA_GG
Cuts at: 0 871 1740
Size: 871 869
BtgI C'CryG_G
Cuts at: 0 399 1740
Size: 399 1341
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 233 1740
Size: 233 1507
BtsI GCAGTG_nn'
Cuts at: 0 514 1060 1740
Size: 514 546 680
Fragments arranged by size:
680 546 514
DraIII CAC_nnn'GTG
Cuts at: 0 213 1235 1740
Size: 213 1022 505
Fragments arranged by size:
1022 505 213
DrdI GACnn_nn'nnGTC
Cuts at: 0 245 1740
Size: 245 1495
EaeI y'GGCC_r
Cuts at: 0 186 498 611 1125 1646 1740
Size: 186 312 113 514 521 94
Fragments arranged by size:
521 514 312 186 113 94
EarI CTCTTCn'nnn_
Cuts at: 0 37 77 82 295 469 1740
Size: 37 40 5 213 174 1271
Fragments arranged by size:
1271 213 174 40 37 5
EcoICRI GAG'CTC
Cuts at: 0 1274 1740
Size: 1274 466
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 1718 1740
Size: 305 1413 22
Fragments arranged by size:
1413 305 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 790 874 1740
Size: 790 84 866
Fragments arranged by size:
866 790 84
EcoO109I rG'GnC_Cy
Cuts at: 0 330 458 1010 1377 1530 1674 1720 1721
Size: 330 128 552 367 153 144 46 1
Cuts at: 1721 1740
Size: 19
Fragments arranged by size:
552 367 330 153 144 128 46 19
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 71 1680 1740
Size: 71 1609 60
Fragments arranged by size:
1609 71 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 547 579 1740
Size: 547 32 1161
Fragments arranged by size:
1161 547 32
KpnI G_GTAC'C
Cuts at: 0 644 1740
Size: 644 1096
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 947 1740
Size: 947 793
MscI TGG'CCA
Cuts at: 0 613 1740
Size: 613 1127
MspA1I CmG'CkG
Cuts at: 0 699 771 864 1221 1740
Size: 699 72 93 357 519
Fragments arranged by size:
699 519 357 93 72
NheI G'CTAG_C
Cuts at: 0 393 1740
Size: 393 1347
NruI TCG'CGA
Cuts at: 0 998 1740
Size: 998 742
NspI r_CATG'y
Cuts at: 0 1686 1740
Size: 1686 54
PasI CC'CwG_GG
Cuts at: 0 985 1740
Size: 985 755
PflMI CCAn_nnn'nTGG
Cuts at: 0 1257 1740
Size: 1257 483
PpuMI rG'GwC_Cy
Cuts at: 0 458 1010 1377 1530 1740
Size: 458 552 367 153 210
Fragments arranged by size:
552 458 367 210 153
PspOMI G'GGCC_C
Cuts at: 0 1720 1740
Size: 1720 20
PstI C_TGCA'G
Cuts at: 0 686 1220 1691 1740
Size: 686 534 471 49
Fragments arranged by size:
686 534 471 49
PvuII CAG'CTG
Cuts at: 0 864 1740
Size: 864 876
SacI G_AGCT'C
Cuts at: 0 1276 1740
Size: 1276 464
SanDI GG'GwC_CC
Cuts at: 0 458 1010 1740
Size: 458 552 730
Fragments arranged by size:
730 552 458
SfcI C'TryA_G
Cuts at: 0 682 1216 1687 1740
Size: 682 534 471 53
Fragments arranged by size:
682 534 471 53
SmaI CCC'GGG
Cuts at: 0 1536 1740
Size: 1536 204
StuI AGG'CCT
Cuts at: 0 1552 1740
Size: 1552 188
StyI C'CwwG_G
Cuts at: 0 425 551 571 1104 1340 1740
Size: 425 126 20 533 236 400
Fragments arranged by size:
533 425 400 236 126 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1202 1740
Size: 1202 538
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 217 249 559 591 1740
Size: 217 32 310 32 1149
Fragments arranged by size:
1149 310 217 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 257 1740
Size: 257 1483
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1485 1740
Size: 1485 255
XmaI C'CCGG_G
Cuts at: 0 1534 1740
Size: 1534 206
XmnI GAAnn'nnTTC
Cuts at: 0 1660 1740
Size: 1660 80
Enzymes that do cut:
AarI Acc65I AclI AcuI AlfI AlwNI ApaI ApoI
AvaI BanI BanII BbsI BbvCI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BsaI BsaAI
BsaBI BseRI BseYI BsiHKAI BsmBI Bsp1286I BspMI BstAPI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I
KpnI MmeI MscI MspA1I NheI NruI NspI PasI
PflMI PpuMI PspOMI PstI PvuII SacI SanDI SfcI
SmaI StuI StyI TaqII TstI Tth111I XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AccI AfeI AflII AflIII AgeI AhdI AleI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BcgI BciVI BclI BfrBI BplI BpuEI BsaHI
BsaWI BsaXI BsgI BsiEI BsiWI BsmI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstZ17I ClaI CspCI DraI EagI EciI EcoRI EcoRV
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
MfeI MluI MslI NaeI NarI NcoI NdeI NgoMIV
NotI NsiI PacI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI
XbaI XhoI ZraI