Restriction map for
pFN21AB6875
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# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AflIII AgeI Alw44I AlwI
AocI ApaI ApaLI AscI AseI
AsnI AspHI AspI AvaI AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbvCI BbvI BcgI BclI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BsaAI BsaBI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
EaeI EagI EarI EclXI Eco52I
Eco81I EcoO109I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HindIII
KasI Ksp632I KspI MaeII MamI
MfeI MflI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TseI Tth111I TthHB8I Van91I
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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Acc65I 165 GGTACC G^GTACC
AcsI 454 AAATTC [GA]^AATT[CT]
AcyI 439 GGCGTC G[GA]^CG[CT]C
AfaI 166 GTAC GT^AC
AhdI 131 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 60 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 308 AGCGCT AGC^GCT
ApoI 454 AAATTC [GA]^AATT[CT]
Asp700I 81 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 165 GGTACC G^GTACC
AspEI 131 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 130 GGACC G^G[AT]CC
BanI 165 GGTACC G^G[CT][GA]CC
BbsI 81 GAAGAC GAAGAC(2/6)
BciVI 118 GGATAC GTATCC(6/5)
BcnI 370 CCCGG CC^[GC]GG
BfaI 109 CTAG C^TAG
BpuAI 81 GAAGAC GAAGAC(2/6)
BsaHI 439 GGCGTC G[GA]^CG[CT]C
BsaI 244 GAGACC GGTCTC(1/5)
BsgI 384 CTGCAC GTGCAG(16/14)
BsmBI 10 GAGACG CGTCTC(1/5)
BsrBI 324 CCGCTC CCGCTC(-3/-3)
BsrI 347 CCAGT ACTGG(1/-1)
BstEII 447 GGTCACC G^GT[ACGT]ACC
BstNI 465 CCAGG CC^[AT]GG
BstPI 447 GGTCACC G^GT[ACGT]ACC
CfoI 309 GCGC GCG^C
DpnII 15 GATC ^GATC
DsaI 201 CCATGG C^C[GA][CT]GG
Eam1105I 131 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 308 AGCGCT AGC^GCT
EcoNI 430 CCTGACGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 447 GGTCACC G^GT[ACGT]ACC
EcoRII 465 CCAGG ^CC[AT]GG
EcoT14I 201 CCATGG C^C[AT][AT]GG
Fnu4HI 361 GCCGC GC^[ACGT]GC
HaeII 308 AGCGCT [GA]GCGC^[CT]
HaeIII 337 GGCC GG^CC
HgaI 440 GCGTC GACGC(5/10)
HhaI 309 GCGC GCG^C
Hin1I 439 GGCGTC G[GA]^CG[CT]C
HinP1I 309 GCGC G^CGC
ItaI 361 GCCGC GC^[ACGT]GC
KpnI 165 GGTACC GGTAC^C
MaeI 109 CTAG C^TAG
MaeIII 448 GTCAC ^GT[ACGT]AC
MboI 15 GATC ^GATC
MspA1I 256 CAGCTG C[AC]G^C[GT]G
MvaI 465 CCAGG CC^[AT]GG
NciI 370 CCCGG CC^[GC]GG
NcoI 201 CCATGG C^CATGG
NdeII 15 GATC ^GATC
PvuII 256 CAGCTG CAG^CTG
RsaI 166 GTAC GT^AC
Sau3AI 15 GATC ^GATC
SfaNI 48 GATGC GCATC(5/9)
SpeI 108 ACTAGT A^CTAGT
StyI 201 CCATGG C^C[AT][AT]GG
Tsp45I 448 GTCAC ^GT[GC]AC
XcmI 248 CCAGACTCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 81 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 324 CCGC CCGC(-3/-1)
362 CCGC
AluI 257 AGCT AG^CT
399 AGCT
BpmI 253 CTCCAG CTGGAG(16/14)
259 CTGGAG
BsaJI 201 CCATGG C^C[ACGT][ACGT]GG
369 CCCCGG
BsiYI 255 CCAGCTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
430 CCTGACGAAGG
BslI 255 CCAGCTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
430 CCTGACGAAGG
BsmAI 10 GAGAC GTCTC(1/5)
244 GAGAC
BssKI 370 CCCGG ^CC[ACGT]GG
465 CCAGG
BstF5I 321 CATCC GGATG(2/0)
69 GGATG
Cac8I 51 GCAAGC GC[ACGT]^[ACGT]GC
364 GCCTGC
Cfr13I 130 GGACC G^G[ACGT]CC
337 GGCCC
DrdI 403 GACCAGCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
433 GACGAAGGCGTC
FokI 321 CATCC GGATG(9/13)
69 GGATG
HapII 116 CCGG C^CGG
371 CCGG
HincII 73 GTTAAC GT[CT]^[GA]AC
268 GTTAAC
HindII 73 GTTAAC GT[CT]^[GA]AC
268 GTTAAC
HpaI 73 GTTAAC GTT^AAC
268 GTTAAC
HpaII 116 CCGG C^CGG
371 CCGG
MseI 74 TTAA T^TAA
269 TTAA
MslI 387 CACAGCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
420 CACCAATATG
MspI 116 CCGG C^CGG
371 CCGG
MwoI 300 GCAGGACAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
391 GCGATGGAAGC
PleI 251 GACTC GAGTC(4/5)
355 GAGTC
Sau96I 130 GGACC G^G[ACGT]CC
337 GGCCC
ScrFI 370 CCCGG CC^[ACGT]GG
465 CCAGG
TfiI 62 GAATC G^A[AT]TC
197 GAATC
Tru9I 74 TTAA T^TAA
269 TTAA
Tsp509I 142 AATT ^AATT
455 AATT
TspRI 276 CACTG CA[GC]TG[ACGT][ACGT]^
333 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
#########################################
DdeI HinfI HphI MboII MnlI
NlaIII NlaIV